(Circular) (Six-base) MAPSORT of: pFN21AA0459.seq Check: 2573 from: 1 to: 5899 pFN21AA0459 5899 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2599 2599 Size: 5899 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3709 278 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3242 1937 Size: 1305 4594 Acc65I G'GTAC_C Cuts at: 3220 3220 Size: 5899 AclI AA'CG_TT Cuts at: 1563 4026 4399 1563 Size: 2463 373 3063 Fragments arranged by size: 3063 2463 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2219 2697 2897 3962 5346 5614 1632 Size: 587 478 200 1065 1384 268 1917 Fragments arranged by size: 1917 1384 1065 587 478 268 200 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5702 AflIII A'CryG_T Cuts at: 1203 4798 5775 1203 Size: 3595 977 1327 Fragments arranged by size: 3595 1327 977 AgeI A'CCGG_T Cuts at: 4730 4730 Size: 5899 AhdI GACnn_n'nnGTC Cuts at: 4628 4628 Size: 5899 AleI CACnn'nnGTG Cuts at: 1166 3170 1166 Size: 2004 3895 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5899 AlwNI CAG_nnn'CTG Cuts at: 2191 2767 3642 5214 2191 Size: 576 875 1572 2876 Fragments arranged by size: 2876 1572 875 576 ApaI G_GGCC'C Cuts at: 2101 2101 Size: 5899 ApaLI G'TGCA_C Cuts at: 3956 5112 3956 Size: 1156 4743 ApoI r'AATT_y Cuts at: 1493 3208 3434 3586 5695 1493 Size: 1715 226 152 2109 1697 Fragments arranged by size: 2109 1715 1697 226 152 AscI GG'CGCG_CC Cuts at: 2090 2090 Size: 5899 AseI AT'TA_AT Cuts at: 160 1032 4453 160 Size: 872 3421 1606 Fragments arranged by size: 3421 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 AvaI C'yCGr_G Cuts at: 1870 1943 3224 1870 Size: 73 1281 4545 Fragments arranged by size: 4545 1281 73 BamHI G'GATC_C Cuts at: 3229 3596 3229 Size: 367 5532 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2212 2587 2915 3087 3220 Size: 325 217 627 425 375 328 172 133 Cuts at: 3220 4675 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 425 375 328 325 217 172 133 BanII G_rGCy'C Cuts at: 1391 2101 2245 2862 3218 1391 Size: 710 144 617 356 4072 Fragments arranged by size: 4072 710 617 356 144 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2685 3182 928 Size: 971 786 497 3645 Fragments arranged by size: 3645 971 786 497 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4090 4124 1903 Size: 34 2153 34 3678 Fragments arranged by size: 3678 2153 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3791 5001 3791 Size: 1210 4689 BclI T'GATC_A Cuts at: 1548 2251 1548 Size: 703 5196 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2037 2384 4510 Size: 107 122 71 631 605 365 347 2126 Cuts at: 4510 136 Size: 1525 Fragments arranged by size: 2126 1525 631 605 365 347 122 107 71 BglII A'GATC_T Cuts at: 5894 5894 Size: 5899 BlpI GC'TnA_GC Cuts at: 3316 3316 Size: 5899 Bme1580I G_kGCm'C Cuts at: 1790 2101 3960 5116 1790 Size: 311 1859 1156 2573 Fragments arranged by size: 2573 1859 1156 311 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5899 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2856 3630 4588 Size: 416 53 83 399 24 1439 774 958 Cuts at: 4588 442 Size: 1753 Fragments arranged by size: 1753 1439 958 774 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5899 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2284 2284 Size: 5899 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2585 3125 4559 1239 Size: 1346 540 1434 2579 Fragments arranged by size: 2579 1434 1346 540 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2267 2876 4024 4889 5187 2267 Size: 609 1148 865 298 2979 Fragments arranged by size: 2979 1148 865 609 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4562 882 Size: 617 3063 2219 Fragments arranged by size: 3063 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3690 493 Size: 711 2486 2702 Fragments arranged by size: 2702 2486 711 BsaBI GATnn'nnATC Cuts at: 5893 5893 Size: 5899 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3706 4088 4723 275 Size: 53 83 186 936 2173 382 635 1451 Fragments arranged by size: 2173 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4332 4730 5004 5151 5562 4332 Size: 398 274 147 411 4669 Fragments arranged by size: 4669 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5674 Fragments arranged by size: 5674 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2168 2171 2915 3095 1186 Size: 982 3 744 180 3990 Fragments arranged by size: 3990 982 744 180 3 BseYI C'CCAG_C Cuts at: 5102 5598 5102 Size: 496 5403 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2577 1163 Size: 573 841 4485 Fragments arranged by size: 4485 841 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2036 2126 3349 4110 5138 1540 Size: 275 186 35 90 1223 761 1028 2301 Fragments arranged by size: 2301 1223 1028 761 275 186 90 35 BsiHKAI G_wGCw'C Cuts at: 2245 2550 3960 4045 5116 2245 Size: 305 1410 85 1071 3028 Fragments arranged by size: 3028 1410 1071 305 85 BsmI GAATG_Cn' Cuts at: 3410 3503 3410 Size: 93 5806 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2101 2245 2550 2862 3218 3960 4045 Size: 399 311 144 305 312 356 742 85 Cuts at: 4045 5116 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 356 312 311 305 144 85 BspEI T'CCGG_A Cuts at: 5562 5562 Size: 5899 BspHI T'CATG_A Cuts at: 3788 5518 3788 Size: 1730 4169 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2599 3151 3254 844 Size: 1755 552 103 3489 Fragments arranged by size: 3489 1755 552 103 BsrBI CCG'CTC Cuts at: 1394 1823 2083 2113 3188 3786 1394 Size: 429 260 30 1075 598 3507 Fragments arranged by size: 3507 1075 598 429 260 30 BsrDI GCAATG_nn' Cuts at: 65 1248 2416 4394 4568 65 Size: 1183 1168 1978 174 1396 Fragments arranged by size: 1978 1396 1183 1168 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2031 4543 4730 1700 Size: 110 221 2512 187 2869 Fragments arranged by size: 2869 2512 221 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5899 BssHII G'CGCG_C Cuts at: 1925 2090 1925 Size: 165 5734 BssSI C'ACGA_G Cuts at: 3959 4971 3959 Size: 1012 4887 BstAPI GCAn_nnn'nTGC Cuts at: 794 2804 794 Size: 2010 3889 BstBI TT'CG_AA Cuts at: 4703 4703 Size: 5899 BstEII G'GTnAC_C Cuts at: 1184 3163 1184 Size: 1979 3920 BstYI r'GATC_y Cuts at: 1972 3229 3596 3981 3998 5450 5536 5548 5894 Size: 1257 367 385 17 1452 86 12 346 Cuts at: 5894 1972 Size: 1977 Fragments arranged by size: 1977 1452 1257 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5347 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2512 487 Size: 683 56 1286 3874 Fragments arranged by size: 3874 1286 683 56 BtsI GCAGTG_nn' Cuts at: 2888 2917 3059 3415 4209 4229 2888 Size: 29 142 356 794 20 4558 Fragments arranged by size: 4558 794 356 142 29 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5899 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5864 DraI TTT'AAA Cuts at: 3203 3203 Size: 5899 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4906 809 Size: 507 3590 1802 Fragments arranged by size: 3590 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2033 2103 2123 Size: 54 1425 50 275 18 203 70 20 Cuts at: 2123 2376 3346 4235 5864 8 Size: 253 970 889 1629 43 Fragments arranged by size: 1629 1425 970 889 275 253 203 70 54 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 2033 2123 3346 1537 Size: 275 221 90 1223 4090 Fragments arranged by size: 4090 1223 275 221 90 EarI CTCTTCn'nnn_ Cuts at: 1353 2021 2825 2841 3829 1353 Size: 668 804 16 988 3423 Fragments arranged by size: 3423 988 804 668 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2131 4486 4860 5006 387 Size: 1064 138 542 2355 374 146 1280 Fragments arranged by size: 2355 1280 1064 542 374 146 138 EcoICRI GAG'CTC Cuts at: 2243 2243 Size: 5899 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2219 2585 2697 2897 3125 3962 4559 Size: 393 587 366 112 200 228 837 597 Cuts at: 4559 5346 5614 1239 Size: 787 268 1524 Fragments arranged by size: 1524 837 787 597 587 393 366 268 228 200 112 EcoNI CCTnn'n_nnAGG Cuts at: 2105 2105 Size: 5899 EcoO109I rG'GnC_Cy Cuts at: 2098 2188 2098 Size: 90 5809 EcoRI G'AATT_C Cuts at: 3208 3586 3208 Size: 378 5521 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2870 2870 Size: 5899 FspI TGC'GCA Cuts at: 4405 4405 Size: 5899 HaeII r_GCGC'y Cuts at: 1678 5046 1678 Size: 3368 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2608 2637 2640 2669 4540 Size: 32 135 32 1036 29 3 29 1871 Cuts at: 4540 4572 4614 4646 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1871 1036 135 42 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 3243 3489 669 Size: 988 281 1305 246 3079 Fragments arranged by size: 3079 1305 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5899 HpaI GTT'AAC Cuts at: 3489 3489 Size: 5899 KpnI G_GTAC'C Cuts at: 3224 3224 Size: 5899 MfeI C'AATT_G Cuts at: 2227 2255 2418 3498 2227 Size: 28 163 1080 4628 Fragments arranged by size: 4628 1080 163 28 MluI A'CGCG_T Cuts at: 5775 5775 Size: 5899 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5013 5197 1305 Size: 3708 184 2007 Fragments arranged by size: 3708 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2105 2378 10 Size: 54 1425 343 273 273 3531 Fragments arranged by size: 3531 1425 343 273 273 54 MslI CAynn'nnrTG Cuts at: 518 1166 2628 3170 3857 4216 4375 518 Size: 648 1462 542 687 359 159 2042 Fragments arranged by size: 2042 1462 687 648 542 359 159 MspA1I CmG'CkG Cuts at: 1606 2043 2182 2323 3315 3992 5140 5385 1606 Size: 437 139 141 992 677 1148 245 2120 Fragments arranged by size: 2120 1148 992 677 437 245 141 139 NaeI GCC'GGC Cuts at: 2033 2033 Size: 5899 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5347 NdeI CA'TA_TG Cuts at: 387 387 Size: 5899 NgoMIV G'CCGG_C Cuts at: 2031 2031 Size: 5899 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5899 NotI GC'GGCC_GC Cuts at: 3346 3346 Size: 5899 NspI r_CATG'y Cuts at: 1132 1229 2375 3257 4802 1132 Size: 97 1146 882 1545 2229 Fragments arranged by size: 2229 1545 1146 882 97 PasI CC'CwG_GG Cuts at: 1348 2191 1348 Size: 843 5056 PciI A'CATG_T Cuts at: 4798 4798 Size: 5899 PflMI CCAn_nnn'nTGG Cuts at: 1108 2767 1108 Size: 1659 4240 PmeI GTTT'AAAC Cuts at: 3203 3203 Size: 5899 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2144 2176 3968 4000 5481 5513 2144 Size: 32 1792 32 1481 32 2530 Fragments arranged by size: 2530 1792 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5899 PsiI TTA'TAA Cuts at: 2784 3469 2784 Size: 685 5214 PspOMI G'GGCC_C Cuts at: 2097 2097 Size: 5899 PstI C_TGCA'G Cuts at: 830 2187 3251 830 Size: 1357 1064 3478 Fragments arranged by size: 3478 1357 1064 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5899 PvuII CAG'CTG Cuts at: 2182 2182 Size: 5899 SacI G_AGCT'C Cuts at: 2245 2245 Size: 5899 SalI G'TCGA_C Cuts at: 1936 3241 1936 Size: 1305 4594 SapI GCTCTTCn'nnn_ Cuts at: 2021 2021 Size: 5899 SbfI CC_TGCA'GG Cuts at: 3251 3251 Size: 5899 ScaI AGT'ACT Cuts at: 4147 5859 4147 Size: 1712 4187 SfcI C'TryA_G Cuts at: 826 1045 2183 3247 4382 5063 5254 826 Size: 219 1138 1064 1135 681 191 1471 Fragments arranged by size: 1471 1138 1135 1064 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5899 SmaI CCC'GGG Cuts at: 1872 3226 1872 Size: 1354 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2282 2855 4003 4904 5166 820 Size: 197 926 339 573 1148 901 262 1553 Fragments arranged by size: 1553 1148 926 901 573 339 262 197 SnaBI TAC'GTA Cuts at: 493 3690 493 Size: 3197 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5899 SphI G_CATG'C Cuts at: 1132 3257 1132 Size: 2125 3774 SspI AAT'ATT Cuts at: 5 52 2788 3823 5 Size: 47 2736 1035 2081 Fragments arranged by size: 2736 2081 1035 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5899 StyI C'CwwG_G Cuts at: 513 1065 2106 2824 3168 3338 513 Size: 552 1041 718 344 170 3074 Fragments arranged by size: 3074 1041 718 552 344 170 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2972 3926 3943 4096 4281 4726 325 Size: 1255 1392 954 17 153 185 445 1498 Fragments arranged by size: 1498 1392 1255 954 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4145 5857 96 Size: 274 80 33 51 1443 2168 1712 138 Fragments arranged by size: 2168 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5859 5891 5859 Size: 32 5867 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5899 XbaI T'CTAG_A Cuts at: 3235 3235 Size: 5899 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5899 XmaI C'CCGG_G Cuts at: 1870 3224 1870 Size: 1354 4545 XmnI GAAnn'nnTTC Cuts at: 3179 4028 3179 Size: 849 5050 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3707 276 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI