Restriction map for
pFN21AA0459
(Linear) (Six-base) MAPSORT of: KIAA0459.seq Check: 5441 from: 1 to: 1194
KIAA0459.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 594 1194
Size: 594 600
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 692 892 1194
Size: 214 478 200 302
Fragments arranged by size:
478 302 214 200
AleI CACnn'nnGTG
Cuts at: 0 1165 1194
Size: 1165 29
AlwNI CAG_nnn'CTG
Cuts at: 0 186 762 1194
Size: 186 576 432
Fragments arranged by size:
576 432 186
ApaI G_GGCC'C
Cuts at: 0 96 1194
Size: 96 1098
AscI GG'CGCG_CC
Cuts at: 0 85 1194
Size: 85 1109
BanI G'GyrC_C
Cuts at: 0 207 582 910 1082 1194
Size: 207 375 328 172 112
Fragments arranged by size:
375 328 207 172 112
BanII G_rGCy'C
Cuts at: 0 96 240 857 1194
Size: 96 144 617 337
Fragments arranged by size:
617 337 144 96
BbsI GAAGACnn'nnnn_
Cuts at: 0 680 1177 1194
Size: 680 497 17
Fragments arranged by size:
680 497 17
BclI T'GATC_A
Cuts at: 0 246 1194
Size: 246 948
BglI GCCn_nnn'nGGC
Cuts at: 0 32 379 1194
Size: 32 347 815
Fragments arranged by size:
815 347 32
Bme1580I G_kGCm'C
Cuts at: 0 96 1194
Size: 96 1098
BmrI ACTGGGnnnn_n'
Cuts at: 0 851 1194
Size: 851 343
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 279 1194
Size: 279 915
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 1120 1194
Size: 580 540 74
Fragments arranged by size:
580 540 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 871 1194
Size: 262 609 323
Fragments arranged by size:
609 323 262
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 166 910 1090 1194
Size: 163 3 744 180 104
Fragments arranged by size:
744 180 163 104 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 1194
Size: 572 622
BsiEI CG_ry'CG
Cuts at: 0 31 121 1194
Size: 31 90 1073
Fragments arranged by size:
1073 90 31
BsiHKAI G_wGCw'C
Cuts at: 0 240 545 1194
Size: 240 305 649
Fragments arranged by size:
649 305 240
Bsp1286I G_dGCh'C
Cuts at: 0 96 240 545 857 1194
Size: 96 144 305 312 337
Fragments arranged by size:
337 312 305 144 96
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 594 1146 1194
Size: 594 552 48
Fragments arranged by size:
594 552 48
BsrBI CCG'CTC
Cuts at: 0 78 108 1183 1194
Size: 78 30 1075 11
Fragments arranged by size:
1075 78 30 11
BsrDI GCAATG_nn'
Cuts at: 0 411 1194
Size: 411 783
BsrFI r'CCGG_y
Cuts at: 0 26 1194
Size: 26 1168
BssHII G'CGCG_C
Cuts at: 0 85 1194
Size: 85 1109
BstAPI GCAn_nnn'nTGC
Cuts at: 0 799 1194
Size: 799 395
BstEII G'GTnAC_C
Cuts at: 0 1158 1194
Size: 1158 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 507 1194
Size: 507 687
BtsI GCAGTG_nn'
Cuts at: 0 883 912 1054 1194
Size: 883 29 142 140
Fragments arranged by size:
883 142 140 29
EaeI y'GGCC_r
Cuts at: 0 28 98 118 371 1194
Size: 28 70 20 253 823
Fragments arranged by size:
823 253 70 28 20
EagI C'GGCC_G
Cuts at: 0 28 118 1194
Size: 28 90 1076
Fragments arranged by size:
1076 90 28
EarI CTCTTCn'nnn_
Cuts at: 0 16 820 836 1194
Size: 16 804 16 358
Fragments arranged by size:
804 358 16 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 126 1194
Size: 126 1068
EcoICRI GAG'CTC
Cuts at: 0 238 1194
Size: 238 956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 580 692 892 1120 1194
Size: 214 366 112 200 228 74
Fragments arranged by size:
366 228 214 200 112 74
EcoNI CCTnn'n_nnAGG
Cuts at: 0 100 1194
Size: 100 1094
EcoO109I rG'GnC_Cy
Cuts at: 0 93 183 1194
Size: 93 90 1011
Fragments arranged by size:
1011 93 90
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 865 1194
Size: 865 329
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 603 632 635 664 1194
Size: 603 29 3 29 530
Fragments arranged by size:
603 530 29 29 3
MfeI C'AATT_G
Cuts at: 0 222 250 413 1194
Size: 222 28 163 781
Fragments arranged by size:
781 222 163 28
MscI TGG'CCA
Cuts at: 0 100 373 1194
Size: 100 273 821
Fragments arranged by size:
821 273 100
MslI CAynn'nnrTG
Cuts at: 0 623 1165 1194
Size: 623 542 29
Fragments arranged by size:
623 542 29
MspA1I CmG'CkG
Cuts at: 0 38 177 318 1194
Size: 38 139 141 876
Fragments arranged by size:
876 141 139 38
NaeI GCC'GGC
Cuts at: 0 28 1194
Size: 28 1166
NgoMIV G'CCGG_C
Cuts at: 0 26 1194
Size: 26 1168
NspI r_CATG'y
Cuts at: 0 370 1194
Size: 370 824
PasI CC'CwG_GG
Cuts at: 0 186 1194
Size: 186 1008
PflMI CCAn_nnn'nTGG
Cuts at: 0 762 1194
Size: 762 432
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 139 171 1194
Size: 139 32 1023
Fragments arranged by size:
1023 139 32
PsiI TTA'TAA
Cuts at: 0 779 1194
Size: 779 415
PspOMI G'GGCC_C
Cuts at: 0 92 1194
Size: 92 1102
PstI C_TGCA'G
Cuts at: 0 182 1194
Size: 182 1012
PvuII CAG'CTG
Cuts at: 0 177 1194
Size: 177 1017
SacI G_AGCT'C
Cuts at: 0 240 1194
Size: 240 954
SapI GCTCTTCn'nnn_
Cuts at: 0 16 1194
Size: 16 1178
SfcI C'TryA_G
Cuts at: 0 178 1194
Size: 178 1016
SmlI C'TyrA_G
Cuts at: 0 277 850 1194
Size: 277 573 344
Fragments arranged by size:
573 344 277
SspI AAT'ATT
Cuts at: 0 783 1194
Size: 783 411
StyI C'CwwG_G
Cuts at: 0 101 819 1163 1194
Size: 101 718 344 31
Fragments arranged by size:
718 344 101 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 967 1194
Size: 967 227
XmnI GAAnn'nnTTC
Cuts at: 0 1174 1194
Size: 1174 20
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaI AscI BanI BanII
BbsI BclI BglI Bme1580I BmrI BplI BpmI BpuEI
BseRI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssHII BstAPI BstEII BtgZI BtsI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I
MfeI MscI MslI MspA1I NaeI NgoMIV NspI PasI
PflMI PpiI PsiI PspOMI PstI PvuII SacI SapI
SfcI SmlI SspI StyI TaqII XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI
BglII BlpI BmgBI BmtI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiWI BsmI BsmBI BspEI
BspHI BsrGI BssSI BstBI BstXI BstYI BstZ17I Bsu36I
BtgI ClaI CspCI DraI DraIII DrdI EcoRI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MluI MmeI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PfoI PmeI PmlI PpuMI
PshAI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI