(Circular) (Six-base) MAPSORT of: pFN21AA0424.seq Check: 6765 from: 1 to: 6253 pFN21AA0424 6253 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2106 4063 278 Size: 53 83 186 936 570 1957 2468 Fragments arranged by size: 2468 1957 936 570 186 83 53 AccI GT'mk_AC Cuts at: 1937 2780 3596 1937 Size: 843 816 4594 Fragments arranged by size: 4594 843 816 Acc65I G'GTAC_C Cuts at: 3574 3574 Size: 6253 AclI AA'CG_TT Cuts at: 1563 4380 4753 1563 Size: 2817 373 3063 Fragments arranged by size: 3063 2817 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2414 2459 3511 4316 5700 5968 1632 Size: 782 45 1052 805 1384 268 1917 Fragments arranged by size: 1917 1384 1052 805 782 268 45 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6056 AflIII A'CryG_T Cuts at: 1203 2418 5152 6129 1203 Size: 1215 2734 977 1327 Fragments arranged by size: 2734 1327 1215 977 AgeI A'CCGG_T Cuts at: 5084 5084 Size: 6253 AhdI GACnn_n'nnGTC Cuts at: 4982 4982 Size: 6253 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6253 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6253 AlwNI CAG_nnn'CTG Cuts at: 3445 3996 5568 3445 Size: 551 1572 4130 Fragments arranged by size: 4130 1572 551 ApaI G_GGCC'C Cuts at: 2232 2232 Size: 6253 ApaLI G'TGCA_C Cuts at: 4310 5466 4310 Size: 1156 5097 ApoI r'AATT_y Cuts at: 1493 3312 3562 3788 3940 6049 1493 Size: 1819 250 226 152 2109 1697 Fragments arranged by size: 2109 1819 1697 250 226 152 AseI AT'TA_AT Cuts at: 160 1032 4807 160 Size: 872 3775 1606 Fragments arranged by size: 3775 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6253 AvaI C'yCGr_G Cuts at: 1870 1943 3578 1870 Size: 73 1635 4545 Fragments arranged by size: 4545 1635 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3200 3233 3200 Size: 33 6220 BamHI G'GATC_C Cuts at: 3583 3950 3583 Size: 367 5886 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3574 5029 618 Size: 325 217 627 1787 1455 1842 Fragments arranged by size: 1842 1787 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2232 2628 2772 2954 3572 1391 Size: 841 396 144 182 618 4072 Fragments arranged by size: 4072 841 618 396 182 144 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3013 928 Size: 971 1114 4168 Fragments arranged by size: 4168 1114 971 BbvCI CC'TCA_GC Cuts at: 2043 2534 2043 Size: 491 5762 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4444 4478 1903 Size: 34 2507 34 3678 Fragments arranged by size: 3678 2507 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2760 4145 5355 2760 Size: 1385 1210 3658 Fragments arranged by size: 3658 1385 1210 BclI T'GATC_A Cuts at: 1548 2016 1548 Size: 468 5785 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4864 136 Size: 107 122 71 631 605 3192 1525 Fragments arranged by size: 3192 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2739 6248 2739 Size: 3509 2744 BlpI GC'TnA_GC Cuts at: 2766 3670 2766 Size: 904 5349 Bme1580I G_kGCm'C Cuts at: 1790 2232 4314 5470 1790 Size: 442 2082 1156 2573 Fragments arranged by size: 2573 2082 1156 442 BmgBI CAC'GTC Cuts at: 1318 2062 1318 Size: 744 5509 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2932 2979 3984 Size: 416 53 83 399 24 1515 47 1005 Cuts at: 3984 4942 442 Size: 958 1753 Fragments arranged by size: 1753 1515 1005 958 416 399 83 53 47 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6253 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2043 2522 2712 3254 4913 1239 Size: 804 479 190 542 1659 2579 Fragments arranged by size: 2579 1659 804 542 479 190 Bpu10I CC'TnA_GC Cuts at: 2043 2534 3254 3363 2043 Size: 491 720 109 4933 Fragments arranged by size: 4933 720 491 109 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2354 4378 5243 5541 2354 Size: 2024 865 298 3066 Fragments arranged by size: 3066 2024 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2490 4916 882 Size: 617 991 2426 2219 Fragments arranged by size: 2426 2219 991 617 BsaAI yAC'GTr Cuts at: 493 1204 4044 493 Size: 711 2840 2702 Fragments arranged by size: 2840 2702 711 BsaBI GATnn'nnATC Cuts at: 6247 6247 Size: 6253 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 4060 4442 5077 Size: 53 83 186 936 570 1957 382 635 Cuts at: 5077 275 Size: 1451 Fragments arranged by size: 1957 1451 936 635 570 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3069 4686 5084 5358 5505 5916 3069 Size: 1617 398 274 147 411 3406 Fragments arranged by size: 3406 1617 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2156 2186 2495 2525 1178 Size: 30 165 30 753 30 309 30 4906 Fragments arranged by size: 4906 753 309 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2177 2237 3218 1186 Size: 991 60 981 4221 Fragments arranged by size: 4221 991 981 60 BseYI C'CCAG_C Cuts at: 2231 5456 5952 2231 Size: 3225 496 2532 Fragments arranged by size: 3225 2532 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2261 2753 1163 Size: 573 525 492 4663 Fragments arranged by size: 4663 573 525 492 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3004 3703 4464 5492 1540 Size: 275 186 1003 699 761 1028 2301 Fragments arranged by size: 2301 1028 1003 761 699 275 186 BsiHKAI G_wGCw'C Cuts at: 2345 2565 2628 2772 2954 4314 4399 5470 2345 Size: 220 63 144 182 1360 85 1071 3128 Fragments arranged by size: 3128 1360 1071 220 182 144 85 63 BsmI GAATG_Cn' Cuts at: 3178 3764 3857 3178 Size: 586 93 5574 Fragments arranged by size: 5574 586 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2232 2345 2565 2628 2772 2954 3572 Size: 399 442 113 220 63 144 182 618 Cuts at: 3572 4314 4399 5470 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 618 442 399 220 182 144 113 85 63 BspEI T'CCGG_A Cuts at: 3069 5916 3069 Size: 2847 3406 BspHI T'CATG_A Cuts at: 2832 4142 5872 2832 Size: 1310 1730 3213 Fragments arranged by size: 3213 1730 1310 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3463 3522 3608 844 Size: 2619 59 86 3489 Fragments arranged by size: 3489 2619 86 59 BsrBI CCG'CTC Cuts at: 1394 1823 3390 4140 1394 Size: 429 1567 750 3507 Fragments arranged by size: 3507 1567 750 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2700 3348 4748 4922 65 Size: 1183 1452 648 1400 174 1396 Fragments arranged by size: 1452 1400 1396 1183 648 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4897 5084 1700 Size: 110 3087 187 2869 Fragments arranged by size: 3087 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6253 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6253 BssSI C'ACGA_G Cuts at: 4313 5325 4313 Size: 1012 5241 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6253 BstBI TT'CG_AA Cuts at: 5057 5057 Size: 6253 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6253 BstXI CCAn_nnnn'nTGG Cuts at: 3232 3232 Size: 6253 BstYI r'GATC_y Cuts at: 1972 2739 2985 3583 3950 4335 4352 5804 5890 Size: 767 246 598 367 385 17 1452 86 Cuts at: 5890 5902 6248 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 767 598 385 367 346 246 86 17 12 BstZ17I GTA'TAC Cuts at: 2781 2781 Size: 6253 BtgI C'CryG_G Cuts at: 513 1065 2067 3433 513 Size: 552 1002 1366 3333 Fragments arranged by size: 3333 1366 1002 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2250 3445 487 Size: 683 56 1024 1195 3295 Fragments arranged by size: 3295 1195 1024 683 56 BtsI GCAGTG_nn' Cuts at: 2405 3769 4563 4583 2405 Size: 1364 794 20 4075 Fragments arranged by size: 4075 1364 794 20 ClaI AT'CG_AT Cuts at: 1554 2709 1554 Size: 1155 5098 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6218 DraI TTT'AAA Cuts at: 2092 3557 2092 Size: 1465 4788 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3158 5260 809 Size: 507 1842 2102 1802 Fragments arranged by size: 2102 1842 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 2288 3001 Size: 54 1425 50 275 18 240 218 713 Cuts at: 3001 3436 3700 4589 6218 8 Size: 435 264 889 1629 43 Fragments arranged by size: 1629 1425 889 713 435 275 264 240 218 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3001 3700 1537 Size: 275 1189 699 4090 Fragments arranged by size: 4090 1189 699 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2402 3264 3323 4183 1353 Size: 1049 862 59 860 3423 Fragments arranged by size: 3423 1049 862 860 59 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4840 5214 5360 387 Size: 1064 138 3251 374 146 1280 Fragments arranged by size: 3251 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2626 2770 2952 2626 Size: 144 182 5927 Fragments arranged by size: 5927 182 144 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2043 2414 2459 2522 2712 3254 3511 Size: 393 411 371 45 63 190 542 257 Cuts at: 3511 4316 4913 5700 5968 1239 Size: 805 597 787 268 1524 Fragments arranged by size: 1524 805 787 597 542 411 393 371 268 257 190 63 45 EcoO109I rG'GnC_Cy Cuts at: 2228 2546 3061 3421 2228 Size: 318 515 360 5060 Fragments arranged by size: 5060 515 360 318 EcoRI G'AATT_C Cuts at: 3562 3940 3562 Size: 378 5875 EcoRV GAT'ATC Cuts at: 2467 2467 Size: 6253 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2464 2770 3193 2464 Size: 306 423 5524 Fragments arranged by size: 5524 423 306 FspI TGC'GCA Cuts at: 4759 4759 Size: 6253 HaeII r_GCGC'y Cuts at: 1678 5400 1678 Size: 3722 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2016 2048 2936 2968 4894 Size: 32 135 32 444 32 888 32 1926 Cuts at: 4894 4926 4968 5000 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1926 888 444 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3127 3379 3537 3597 3843 669 Size: 988 281 1189 252 158 60 246 3079 Fragments arranged by size: 3079 1189 988 281 252 246 158 60 HindIII A'AGCT_T Cuts at: 748 3182 748 Size: 2434 3819 HpaI GTT'AAC Cuts at: 3537 3843 3537 Size: 306 5947 KpnI G_GTAC'C Cuts at: 3578 3578 Size: 6253 MfeI C'AATT_G Cuts at: 3852 3852 Size: 6253 MluI A'CGCG_T Cuts at: 6129 6129 Size: 6253 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2151 5367 5551 1305 Size: 846 3216 184 2007 Fragments arranged by size: 3216 2007 846 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2072 10 Size: 54 1425 343 240 4191 Fragments arranged by size: 4191 1425 343 240 54 MslI CAynn'nnrTG Cuts at: 518 1166 2568 2610 3104 4211 4570 4729 518 Size: 648 1402 42 494 1107 359 159 2042 Fragments arranged by size: 2042 1402 1107 648 494 359 159 42 MspA1I CmG'CkG Cuts at: 1606 2819 3016 3669 4346 5494 5739 1606 Size: 1213 197 653 677 1148 245 2120 Fragments arranged by size: 2120 1213 1148 677 653 245 197 NcoI C'CATG_G Cuts at: 513 1065 2067 513 Size: 552 1002 4699 Fragments arranged by size: 4699 1002 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6253 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6253 NotI GC'GGCC_GC Cuts at: 3700 3700 Size: 6253 NspI r_CATG'y Cuts at: 1132 1229 2422 3611 5156 1132 Size: 97 1193 1189 1545 2229 Fragments arranged by size: 2229 1545 1193 1189 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6253 PciI A'CATG_T Cuts at: 2418 5152 2418 Size: 2734 3519 PflMI CCAn_nnn'nTGG Cuts at: 1108 3043 3445 1108 Size: 1935 402 3916 Fragments arranged by size: 3916 1935 402 PmeI GTTT'AAAC Cuts at: 3557 3557 Size: 6253 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4322 4354 5835 5867 4322 Size: 32 1481 32 4708 Fragments arranged by size: 4708 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2546 3061 3421 2546 Size: 515 360 5378 Fragments arranged by size: 5378 515 360 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6253 PsiI TTA'TAA Cuts at: 3823 3823 Size: 6253 PspOMI G'GGCC_C Cuts at: 2228 2228 Size: 6253 PstI C_TGCA'G Cuts at: 830 3605 830 Size: 2775 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6253 PvuII CAG'CTG Cuts at: 2819 2819 Size: 6253 SacI G_AGCT'C Cuts at: 2628 2772 2954 2628 Size: 144 182 5927 Fragments arranged by size: 5927 182 144 SalI G'TCGA_C Cuts at: 1936 3595 1936 Size: 1659 4594 SbfI CC_TGCA'GG Cuts at: 3605 3605 Size: 6253 ScaI AGT'ACT Cuts at: 4501 6213 4501 Size: 1712 4541 SexAI A'CCwGG_T Cuts at: 3444 3444 Size: 6253 SfcI C'TryA_G Cuts at: 826 1045 2294 2470 2803 3601 4736 5417 5608 Size: 219 1249 176 333 798 1135 681 191 Cuts at: 5608 826 Size: 1471 Fragments arranged by size: 1471 1249 1135 798 681 333 219 191 176 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6253 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6253 SmaI CCC'GGG Cuts at: 1872 3580 1872 Size: 1708 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2369 4357 5258 5520 820 Size: 197 926 426 1988 901 262 1553 Fragments arranged by size: 1988 1553 926 901 426 262 197 SnaBI TAC'GTA Cuts at: 493 4044 493 Size: 3551 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6253 SphI G_CATG'C Cuts at: 1132 3611 1132 Size: 2479 3774 SspI AAT'ATT Cuts at: 5 52 2884 4177 5 Size: 47 2832 1293 2081 Fragments arranged by size: 2832 2081 1293 47 StuI AGG'CCT Cuts at: 1517 2672 1517 Size: 1155 5098 StyI C'CwwG_G Cuts at: 513 1065 2067 3692 513 Size: 552 1002 1625 3074 Fragments arranged by size: 3074 1625 1002 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2188 4280 4297 4450 4635 5080 325 Size: 1255 608 2092 17 153 185 445 1498 Fragments arranged by size: 2092 1498 1255 608 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3084 4499 6211 Size: 274 80 33 51 1443 1107 1415 1712 Cuts at: 6211 96 Size: 138 Fragments arranged by size: 1712 1443 1415 1107 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2126 2158 6213 6245 2126 Size: 32 4055 32 2134 Fragments arranged by size: 4055 2134 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6253 XbaI T'CTAG_A Cuts at: 3589 3589 Size: 6253 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2080 2080 Size: 6253 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6253 XmaI C'CCGG_G Cuts at: 1870 3578 1870 Size: 1708 4545 XmnI GAAnn'nnTTC Cuts at: 4382 4382 Size: 6253 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2104 4061 276 Size: 53 83 186 936 570 1957 2468 Fragments arranged by size: 2468 1957 936 570 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI