(Circular) (Six-base) MAPSORT of: pFN21AA0412.seq Check: 9736 from: 1 to: 6586 pFN21AA0412 6586 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4396 278 Size: 53 83 186 936 2860 2468 Fragments arranged by size: 2860 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3929 1937 Size: 1992 4594 Acc65I G'GTAC_C Cuts at: 3907 3907 Size: 6586 AclI AA'CG_TT Cuts at: 1563 4713 5086 1563 Size: 3150 373 3063 Fragments arranged by size: 3150 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2315 2407 3409 3631 4649 6033 6301 1632 Size: 683 92 1002 222 1018 1384 268 1917 Fragments arranged by size: 1917 1384 1018 1002 683 268 222 92 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6389 AflIII A'CryG_T Cuts at: 1203 5485 6462 1203 Size: 4282 977 1327 Fragments arranged by size: 4282 1327 977 AgeI A'CCGG_T Cuts at: 5417 5417 Size: 6586 AhdI GACnn_n'nnGTC Cuts at: 5315 5315 Size: 6586 AleI CACnn'nnGTG Cuts at: 1166 3018 1166 Size: 1852 4734 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6586 AlwNI CAG_nnn'CTG Cuts at: 4329 5901 4329 Size: 1572 5014 ApaI G_GGCC'C Cuts at: 2035 3349 2035 Size: 1314 5272 ApaLI G'TGCA_C Cuts at: 2953 4643 5799 2953 Size: 1690 1156 3740 Fragments arranged by size: 3740 1690 1156 ApoI r'AATT_y Cuts at: 1493 2673 3895 4121 4273 6382 1493 Size: 1180 1222 226 152 2109 1697 Fragments arranged by size: 2109 1697 1222 1180 226 152 AseI AT'TA_AT Cuts at: 160 1032 5140 160 Size: 872 4108 1606 Fragments arranged by size: 4108 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6586 AvaI C'yCGr_G Cuts at: 1870 1943 3209 3911 1870 Size: 73 1266 702 4545 Fragments arranged by size: 4545 1266 702 73 BamHI G'GATC_C Cuts at: 3916 4283 3916 Size: 367 6219 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3084 3252 3907 5362 618 Size: 325 217 627 1297 168 655 1455 1842 Fragments arranged by size: 1842 1455 1297 655 627 325 217 168 BanII G_rGCy'C Cuts at: 1391 2035 2151 2471 3349 3905 1391 Size: 644 116 320 878 556 4072 Fragments arranged by size: 4072 878 644 556 320 116 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2241 3049 3133 3730 3870 928 Size: 971 342 808 84 597 140 3644 Fragments arranged by size: 3644 971 808 597 342 140 84 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4777 4811 1903 Size: 34 2840 34 3678 Fragments arranged by size: 3678 2840 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4478 5688 4478 Size: 1210 5376 BclI T'GATC_A Cuts at: 1548 3844 1548 Size: 2296 4290 BfrBI ATG'CAT Cuts at: 3685 3685 Size: 6586 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5197 136 Size: 107 122 71 631 605 337 3188 1525 Fragments arranged by size: 3188 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6581 6581 Size: 6586 BlpI GC'TnA_GC Cuts at: 2106 2184 4003 2106 Size: 78 1819 4689 Fragments arranged by size: 4689 1819 78 Bme1580I G_kGCm'C Cuts at: 1790 2035 2957 3349 4647 5803 1790 Size: 245 922 392 1298 1156 2573 Fragments arranged by size: 2573 1298 1156 922 392 245 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6586 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2775 3279 3699 Size: 416 53 83 399 24 1358 504 420 Cuts at: 3699 4317 5275 442 Size: 618 958 1753 Fragments arranged by size: 1753 1358 958 618 504 420 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6586 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2341 2548 2341 Size: 207 6379 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2520 2703 2955 3207 3375 3459 3589 3627 Size: 1281 183 252 252 168 84 130 38 Cuts at: 3627 5246 1239 Size: 1619 2579 Fragments arranged by size: 2579 1619 1281 252 252 183 168 130 84 38 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3230 4711 5576 5874 3230 Size: 1481 865 298 3942 Fragments arranged by size: 3942 1481 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5249 882 Size: 617 3750 2219 Fragments arranged by size: 3750 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4377 493 Size: 711 3173 2702 Fragments arranged by size: 3173 2702 711 BsaBI GATnn'nnATC Cuts at: 6580 6580 Size: 6586 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4393 4775 5410 275 Size: 53 83 186 936 2860 382 635 1451 Fragments arranged by size: 2860 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5019 5417 5691 5838 6249 5019 Size: 398 274 147 411 5356 Fragments arranged by size: 5356 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2532 2562 1178 Size: 30 165 30 1129 30 5202 Fragments arranged by size: 5202 1129 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 1186 Size: 910 5676 BseYI C'CCAG_C Cuts at: 2829 5789 6285 2829 Size: 2960 496 3130 Fragments arranged by size: 3130 2960 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3142 3174 3310 1163 Size: 573 1406 32 136 4439 Fragments arranged by size: 4439 1406 573 136 32 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4036 4797 5825 1540 Size: 275 186 2035 761 1028 2301 Fragments arranged by size: 2301 2035 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2735 2957 3575 4647 4732 5803 2735 Size: 222 618 1072 85 1071 3518 Fragments arranged by size: 3518 1072 1071 618 222 85 BsmI GAATG_Cn' Cuts at: 2705 2882 4097 4190 2705 Size: 177 1215 93 5101 Fragments arranged by size: 5101 1215 177 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2151 2471 2735 2957 3349 3575 Size: 399 245 116 320 264 222 392 226 Cuts at: 3575 3905 4647 4732 5803 1391 Size: 330 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 392 330 320 264 245 226 222 116 85 BspEI T'CCGG_A Cuts at: 6249 6249 Size: 6586 BspHI T'CATG_A Cuts at: 4475 6205 4475 Size: 1730 4856 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3941 844 Size: 3097 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2901 4473 1394 Size: 429 1078 1572 3507 Fragments arranged by size: 3507 1572 1078 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2878 5081 5255 65 Size: 1183 1630 2203 174 1396 Fragments arranged by size: 2203 1630 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5230 5417 1700 Size: 110 3420 187 2869 Fragments arranged by size: 3420 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6586 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6586 BssSI C'ACGA_G Cuts at: 3499 4646 5658 3499 Size: 1147 1012 4427 Fragments arranged by size: 4427 1147 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2877 794 Size: 2083 4503 BstBI TT'CG_AA Cuts at: 5390 5390 Size: 6586 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6586 BstYI r'GATC_y Cuts at: 1972 2437 2581 3916 4283 4668 4685 6137 6223 Size: 465 144 1335 367 385 17 1452 86 Cuts at: 6223 6235 6581 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1335 465 385 367 346 144 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2219 513 Size: 552 939 215 4880 Fragments arranged by size: 4880 939 552 215 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5847 Fragments arranged by size: 5847 683 56 BtsI GCAGTG_nn' Cuts at: 2626 3130 3298 3316 4102 4896 4916 2626 Size: 504 168 18 786 794 20 4296 Fragments arranged by size: 4296 794 786 504 168 20 18 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6586 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3364 3399 529 Size: 35 2800 35 3716 Fragments arranged by size: 3716 2800 35 35 DraI TTT'AAA Cuts at: 2614 3890 2614 Size: 1276 5310 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5593 809 Size: 507 4277 1802 Fragments arranged by size: 4277 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2965 4033 4922 Size: 54 1425 50 275 18 1135 1068 889 Cuts at: 4922 6551 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1135 1068 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4033 1537 Size: 275 2221 4090 Fragments arranged by size: 4090 2221 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2592 4516 1353 Size: 1239 1924 3423 Fragments arranged by size: 3423 1924 1239 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5173 5547 5693 387 Size: 1064 138 3584 374 146 1280 Fragments arranged by size: 3584 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2315 2407 2520 2703 2955 3207 3375 Size: 393 683 92 113 183 252 252 168 Cuts at: 3375 3409 3459 3589 3627 3631 4649 5246 6033 Size: 34 50 130 38 4 1018 597 787 Cuts at: 6033 6301 1239 Size: 268 1524 Fragments arranged by size: 1524 1018 787 683 597 393 268 252 252 183 168 130 113 92 50 38 34 4 EcoNI CCTnn'n_nnAGG Cuts at: 2120 2120 Size: 6586 EcoO109I rG'GnC_Cy Cuts at: 2775 3345 3409 2775 Size: 570 64 5952 Fragments arranged by size: 5952 570 64 EcoRI G'AATT_C Cuts at: 3895 4273 3895 Size: 378 6208 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3160 3160 Size: 6586 FspI TGC'GCA Cuts at: 5092 5092 Size: 6586 HaeII r_GCGC'y Cuts at: 1678 5733 1678 Size: 4055 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2384 2416 2532 2564 2722 Size: 32 135 32 812 32 116 32 158 Cuts at: 2722 2754 3228 3260 3812 3844 5227 5259 5301 Size: 32 474 32 552 32 1383 32 42 Cuts at: 5301 5333 1373 Size: 32 2626 Fragments arranged by size: 2626 1383 812 552 474 158 135 116 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3930 4176 669 Size: 988 281 1992 246 3079 Fragments arranged by size: 3079 1992 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6586 HpaI GTT'AAC Cuts at: 4176 4176 Size: 6586 KpnI G_GTAC'C Cuts at: 3911 3911 Size: 6586 MfeI C'AATT_G Cuts at: 4185 4185 Size: 6586 MluI A'CGCG_T Cuts at: 6462 6462 Size: 6586 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5700 5884 1305 Size: 4395 184 2007 Fragments arranged by size: 4395 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2967 10 Size: 54 1425 343 1135 3629 Fragments arranged by size: 3629 1425 1135 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3018 3171 3485 4544 4903 5062 518 Size: 648 1852 153 314 1059 359 159 2042 Fragments arranged by size: 2042 1852 1059 648 359 314 159 153 MspA1I CmG'CkG Cuts at: 1606 2015 2095 2110 2495 2839 3259 4002 4679 Size: 409 80 15 385 344 420 743 677 Cuts at: 4679 5827 6072 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 743 677 420 409 385 344 245 80 15 NcoI C'CATG_G Cuts at: 513 1065 2004 2219 513 Size: 552 939 215 4880 Fragments arranged by size: 4880 939 552 215 NdeI CA'TA_TG Cuts at: 387 387 Size: 6586 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6586 NotI GC'GGCC_GC Cuts at: 4033 4033 Size: 6586 NsiI A_TGCA'T Cuts at: 3687 3687 Size: 6586 NspI r_CATG'y Cuts at: 1132 1229 3490 3593 3944 5489 1132 Size: 97 2261 103 351 1545 2229 Fragments arranged by size: 2261 2229 1545 351 103 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6586 PciI A'CATG_T Cuts at: 5485 5485 Size: 6586 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6586 PfoI T'CCnGG_A Cuts at: 2536 2536 Size: 6586 PmeI GTTT'AAAC Cuts at: 3890 3890 Size: 6586 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4655 4687 6168 6200 4655 Size: 32 1481 32 5041 Fragments arranged by size: 5041 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3409 3409 Size: 6586 PshAI GACnn'nnGTC Cuts at: 1613 2253 1613 Size: 640 5946 PsiI TTA'TAA Cuts at: 4156 4156 Size: 6586 PspOMI G'GGCC_C Cuts at: 2031 3345 2031 Size: 1314 5272 PspXI vC'TCGA_Gb Cuts at: 3209 3209 Size: 6586 PstI C_TGCA'G Cuts at: 830 3938 830 Size: 3108 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6586 PvuII CAG'CTG Cuts at: 2095 2495 2095 Size: 400 6186 RsrII CG'GwC_CG Cuts at: 2026 2026 Size: 6586 SalI G'TCGA_C Cuts at: 1936 3928 1936 Size: 1992 4594 SbfI CC_TGCA'GG Cuts at: 3938 3938 Size: 6586 ScaI AGT'ACT Cuts at: 4834 6546 4834 Size: 1712 4874 SfcI C'TryA_G Cuts at: 826 1045 3934 5069 5750 5941 826 Size: 219 2889 1135 681 191 1471 Fragments arranged by size: 2889 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6586 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6586 SmaI CCC'GGG Cuts at: 1872 3913 1872 Size: 2041 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3209 3245 4690 5591 5853 820 Size: 197 926 1266 36 1445 901 262 1553 Fragments arranged by size: 1553 1445 1266 926 901 262 197 36 SnaBI TAC'GTA Cuts at: 493 4377 493 Size: 3884 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6586 SphI G_CATG'C Cuts at: 1132 3944 1132 Size: 2812 3774 SspI AAT'ATT Cuts at: 5 52 3811 4510 5 Size: 47 3759 699 2081 Fragments arranged by size: 3759 2081 699 47 StuI AGG'CCT Cuts at: 1517 2804 2888 3308 3476 3644 1517 Size: 1287 84 420 168 168 4459 Fragments arranged by size: 4459 1287 420 168 168 84 StyI C'CwwG_G Cuts at: 513 1065 2004 2076 2219 4025 513 Size: 552 939 72 143 1806 3074 Fragments arranged by size: 3074 1806 939 552 143 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2909 3329 4613 4630 4783 4968 5413 Size: 1255 1329 420 1284 17 153 185 445 Cuts at: 5413 325 Size: 1498 Fragments arranged by size: 1498 1329 1284 1255 445 420 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4832 6544 96 Size: 274 80 33 51 1443 2855 1712 138 Fragments arranged by size: 2855 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2343 2375 2833 2865 3505 3537 6546 6578 2343 Size: 32 458 32 640 32 3009 32 2351 Fragments arranged by size: 3009 2351 640 458 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6586 XbaI T'CTAG_A Cuts at: 3922 3922 Size: 6586 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2204 2204 Size: 6586 XhoI C'TCGA_G Cuts at: 1943 3209 1943 Size: 1266 5320 XmaI C'CCGG_G Cuts at: 1870 3911 1870 Size: 2041 4545 XmnI GAAnn'nnTTC Cuts at: 3140 3224 3281 4715 3140 Size: 84 57 1434 5011 Fragments arranged by size: 5011 1434 84 57 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4394 276 Size: 53 83 186 936 2860 2468 Fragments arranged by size: 2860 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PsrI SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI