(Circular) (Six-base) MAPSORT of: pFN21AA0394.seq Check: 2284 from: 1 to: 5941 pFN21AA0394 5941 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3751 278 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3284 1937 Size: 1347 4594 Acc65I G'GTAC_C Cuts at: 3262 3262 Size: 5941 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2516 4004 5388 5656 1632 Size: 884 1488 1384 268 1917 Fragments arranged by size: 1917 1488 1384 884 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 3114 4840 5817 1203 Size: 1911 1726 977 1327 Fragments arranged by size: 1911 1726 1327 977 AgeI A'CCGG_T Cuts at: 2270 4772 2270 Size: 2502 3439 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 2253 1166 Size: 1087 4854 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2525 1184 Size: 1341 4600 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2276 2308 2466 2498 2276 Size: 32 158 32 5719 Fragments arranged by size: 5719 158 32 32 AlwNI CAG_nnn'CTG Cuts at: 2243 2287 2386 2389 2881 3079 3684 5256 2243 Size: 44 99 3 492 198 605 1572 2928 Fragments arranged by size: 2928 1572 605 492 198 99 44 3 ApaI G_GGCC'C Cuts at: 2641 2641 Size: 5941 ApaLI G'TGCA_C Cuts at: 3998 5154 3998 Size: 1156 4785 ApoI r'AATT_y Cuts at: 1493 2528 3250 3476 3628 5737 1493 Size: 1035 722 226 152 2109 1697 Fragments arranged by size: 2109 1697 1035 722 226 152 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 3266 1870 Size: 73 1323 4545 Fragments arranged by size: 4545 1323 73 BamHI G'GATC_C Cuts at: 2291 3271 3638 2291 Size: 980 367 4594 Fragments arranged by size: 4594 980 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3262 4717 618 Size: 325 217 627 1475 1455 1842 Fragments arranged by size: 1842 1475 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2183 2641 3141 3260 1391 Size: 792 458 500 119 4072 Fragments arranged by size: 4072 792 500 458 119 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2565 2876 928 Size: 971 666 311 3993 Fragments arranged by size: 3993 971 666 311 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2779 2813 4132 4166 1903 Size: 34 842 34 1319 34 3678 Fragments arranged by size: 3678 1319 842 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2198 3833 5043 2198 Size: 1635 1210 3096 Fragments arranged by size: 3096 1635 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4552 136 Size: 107 122 71 631 605 2880 1525 Fragments arranged by size: 2880 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5936 5936 Size: 5941 BlpI GC'TnA_GC Cuts at: 2580 2894 3358 2580 Size: 314 464 5163 Fragments arranged by size: 5163 464 314 Bme1580I G_kGCm'C Cuts at: 1790 2641 4002 5158 1790 Size: 851 1361 1156 2573 Fragments arranged by size: 2573 1361 1156 851 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5941 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2986 3672 4630 Size: 416 53 83 399 24 1569 686 958 Cuts at: 4630 442 Size: 1753 Fragments arranged by size: 1753 1569 958 686 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2635 2975 2635 Size: 340 5601 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2220 2468 2882 2903 2970 3065 4601 1239 Size: 981 248 414 21 67 95 1536 2579 Fragments arranged by size: 2579 1536 981 414 248 95 67 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2666 4066 4931 5229 2666 Size: 1400 865 298 3378 Fragments arranged by size: 3378 1400 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2111 2850 4604 882 Size: 617 612 739 1754 2219 Fragments arranged by size: 2219 1754 739 617 612 BsaAI yAC'GTr Cuts at: 493 1204 3732 493 Size: 711 2528 2702 Fragments arranged by size: 2702 2528 711 BsaBI GATnn'nnATC Cuts at: 2296 5935 2296 Size: 3639 2302 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3748 4130 4765 275 Size: 53 83 186 936 2215 382 635 1451 Fragments arranged by size: 2215 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2270 4374 4772 5046 5193 5604 2270 Size: 2104 398 274 147 411 2607 Fragments arranged by size: 2607 2104 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2466 2496 1178 Size: 30 165 30 1063 30 4623 Fragments arranged by size: 4623 1063 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2924 1186 Size: 1738 4203 BseYI C'CCAG_C Cuts at: 2135 2224 2465 2874 5144 5640 2135 Size: 89 241 409 2270 496 2436 Fragments arranged by size: 2436 2270 496 409 241 89 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5368 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3391 4152 5180 1540 Size: 275 186 1390 761 1028 2301 Fragments arranged by size: 2301 1390 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2183 3204 4002 4087 5158 2183 Size: 1021 798 85 1071 2966 Fragments arranged by size: 2966 1071 1021 798 85 BsmI GAATG_Cn' Cuts at: 3452 3545 3452 Size: 93 5848 BsmBI CGTCTCn'nnnn_ Cuts at: 2946 2946 Size: 5941 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2183 2641 3141 3204 3260 4002 4087 Size: 399 393 458 500 63 56 742 85 Cuts at: 4087 5158 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 500 458 399 393 85 63 56 BspEI T'CCGG_A Cuts at: 5604 5604 Size: 5941 BspHI T'CATG_A Cuts at: 3830 5560 3830 Size: 1730 4211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3296 844 Size: 2452 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2848 3040 3828 1394 Size: 429 1025 192 788 3507 Fragments arranged by size: 3507 1025 788 429 192 BsrDI GCAATG_nn' Cuts at: 65 1248 2775 4436 4610 65 Size: 1183 1527 1661 174 1396 Fragments arranged by size: 1661 1527 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2085 2270 4585 4772 1700 Size: 110 275 185 2315 187 2869 Fragments arranged by size: 2869 2315 275 187 185 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5941 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 4001 5013 4001 Size: 1012 4929 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5941 BstBI TT'CG_AA Cuts at: 3157 4745 3157 Size: 1588 4353 BstEII G'GTnAC_C Cuts at: 1184 3016 1184 Size: 1832 4109 BstXI CCAn_nnnn'nTGG Cuts at: 2058 2403 3028 2058 Size: 345 625 4971 Fragments arranged by size: 4971 625 345 BstYI r'GATC_y Cuts at: 1972 2291 2974 3271 3638 4023 4040 5492 5578 Size: 319 683 297 367 385 17 1452 86 Cuts at: 5578 5590 5936 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 683 385 367 346 319 297 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2079 2079 Size: 5941 BtgI C'CryG_G Cuts at: 513 1065 2004 3141 513 Size: 552 939 1137 3313 Fragments arranged by size: 3313 1137 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2283 487 Size: 683 56 1057 4145 Fragments arranged by size: 4145 1057 683 56 BtsI GCAGTG_nn' Cuts at: 3457 4251 4271 3457 Size: 794 20 5127 Fragments arranged by size: 5127 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3245 3245 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4948 809 Size: 507 3632 1802 Fragments arranged by size: 3632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3171 3388 4277 Size: 54 1425 50 275 18 1341 217 889 Cuts at: 4277 5906 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1341 889 275 217 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3388 1537 Size: 275 1576 4090 Fragments arranged by size: 4090 1576 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2645 3003 3871 1353 Size: 1292 358 868 3423 Fragments arranged by size: 3423 1292 868 358 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2007 2675 3209 4528 4902 5048 Size: 1064 138 418 668 534 1319 374 146 Cuts at: 5048 387 Size: 1280 Fragments arranged by size: 1319 1280 1064 668 534 418 374 146 138 EcoICRI GAG'CTC Cuts at: 2181 2181 Size: 5941 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2220 2468 2516 2882 2903 2970 3065 Size: 393 588 248 48 366 21 67 95 Cuts at: 3065 4004 4601 5388 5656 1239 Size: 939 597 787 268 1524 Fragments arranged by size: 1524 939 787 597 588 393 366 268 248 95 67 48 21 EcoO109I rG'GnC_Cy Cuts at: 2220 2440 2220 Size: 220 5721 EcoRI G'AATT_C Cuts at: 2528 3250 3628 2528 Size: 722 378 4841 Fragments arranged by size: 4841 722 378 FspI TGC'GCA Cuts at: 4447 4447 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 5088 1678 Size: 3410 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2341 2373 4582 4614 4656 Size: 32 135 32 769 32 2209 32 42 Cuts at: 4656 4688 1373 Size: 32 2626 Fragments arranged by size: 2626 2209 769 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2101 3285 3531 669 Size: 988 281 163 1184 246 3079 Fragments arranged by size: 3079 1184 988 281 246 163 HindIII A'AGCT_T Cuts at: 748 2696 748 Size: 1948 3993 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KpnI G_GTAC'C Cuts at: 3266 3266 Size: 5941 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2216 5055 5239 1305 Size: 911 2839 184 2007 Fragments arranged by size: 2839 2007 911 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4119 Fragments arranged by size: 4119 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2253 3899 4258 4417 518 Size: 648 1087 1646 359 159 2042 Fragments arranged by size: 2042 1646 1087 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2386 2494 2881 3097 3151 3357 4034 5182 Size: 780 108 387 216 54 206 677 1148 Cuts at: 5182 5427 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 780 677 387 245 216 206 108 54 NaeI GCC'GGC Cuts at: 2087 2087 Size: 5941 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4450 Fragments arranged by size: 4450 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NgoMIV G'CCGG_C Cuts at: 2085 2085 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 3118 3299 4844 1132 Size: 97 1889 181 1545 2229 Fragments arranged by size: 2229 1889 1545 181 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5941 PciI A'CATG_T Cuts at: 3114 4840 3114 Size: 1726 4215 PflMI CCAn_nnn'nTGG Cuts at: 1108 2287 2881 1108 Size: 1179 594 4168 Fragments arranged by size: 4168 1179 594 PfoI T'CCnGG_A Cuts at: 2535 2535 Size: 5941 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2466 2498 4010 4042 5523 5555 2466 Size: 32 1512 32 1481 32 2852 Fragments arranged by size: 2852 1512 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2220 2440 2220 Size: 220 5721 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5941 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PspOMI G'GGCC_C Cuts at: 2637 2637 Size: 5941 PstI C_TGCA'G Cuts at: 830 3293 830 Size: 2463 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 PvuII CAG'CTG Cuts at: 2386 2494 2881 3097 3151 2386 Size: 108 387 216 54 5176 Fragments arranged by size: 5176 387 216 108 54 SacI G_AGCT'C Cuts at: 2183 2183 Size: 5941 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SanDI GG'GwC_CC Cuts at: 2220 2220 Size: 5941 SapI GCTCTTCn'nnn_ Cuts at: 2645 2645 Size: 5941 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 4189 5901 4189 Size: 1712 4229 SfcI C'TryA_G Cuts at: 826 1045 3289 4424 5105 5296 826 Size: 219 2244 1135 681 191 1471 Fragments arranged by size: 2244 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 2270 1700 Size: 570 5371 SmaI CCC'GGG Cuts at: 1872 3268 1872 Size: 1396 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2681 4045 4946 5208 820 Size: 197 926 738 1364 901 262 1553 Fragments arranged by size: 1553 1364 926 901 738 262 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5941 StyI C'CwwG_G Cuts at: 513 1065 2004 2446 2598 2622 3380 513 Size: 552 939 442 152 24 758 3074 Fragments arranged by size: 3074 939 758 552 442 152 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2376 3968 3985 4138 4323 4768 325 Size: 1255 796 1592 17 153 185 445 1498 Fragments arranged by size: 1592 1498 1255 796 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3087 4187 5899 Size: 274 80 33 51 1443 1110 1100 1712 Cuts at: 5899 96 Size: 138 Fragments arranged by size: 1712 1443 1110 1100 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5901 5933 5901 Size: 32 5909 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5941 XmaI C'CCGG_G Cuts at: 1870 3266 1870 Size: 1396 4545 XmnI GAAnn'nnTTC Cuts at: 4070 4070 Size: 5941 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3749 276 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI