Restriction map for
pFN21AA0369
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BcnI
BfrI BglII BlnI BseAI BsgI
BsiEI BsiWI BsmI BspEI BspHI
BspLU11I BssHII BssSI Bst1107I BstAPI
BstBI BstYI BstZ17I Bsu36I CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoT14I EcoT22I FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MflI MluI
MroI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI Tth111I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 1618 GTAGAC GT^[AC][GT]AC
AcyI 1459 GACGCC G[GA]^CG[CT]C
AflIII 704 ACACGT A^C[GA][CT]GT
AgeI 236 ACCGGT A^CCGGT
AlwI 1296 GATCC GGATC(4/5)
BanI 1645 GGCACC G^G[CT][GA]CC
BciVI 1687 GGATAC GTATCC(6/5)
BclI 1794 TGATCA T^GATCA
BglI 256 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 147 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 147 GCTCAGC GC^T[ACGT]AGC
BsaAI 1657 TACGTG [CT]AC^GT[GA]
BsaBI 1891 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1459 GACGCC G[GA]^CG[CT]C
BsaI 1412 GAGACC GGTCTC(1/5)
BsmBI 1457 GAGACG CGTCTC(1/5)
BsmFI 918 GTCCC GGGAC(10/14)
BspDI 197 ATCGAT AT^CGAT
BsrDI 1674 CATTGC GCAATG(2/0)
BsrFI 236 ACCGGT [GA]^CCGG[CT]
BstEII 561 GGTCACC G^GT[ACGT]ACC
BstPI 561 GGTCACC G^GT[ACGT]ACC
BstXI 668 CCATCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 147 GCTCAGC GC^T[ACGT]AGC
Cfr10I 236 ACCGGT [GA]^CCGG[CT]
ClaI 197 ATCGAT AT^CGAT
EarI 1322 GAAGAG CTCTTC(1/4)
EcoO65I 561 GGTCACC G^GT[ACGT]ACC
FbaI 1794 TGATCA T^GATCA
Hin1I 1459 GACGCC G[GA]^CG[CT]C
Ksp632I 1322 GAAGAG CTCTTC(1/4)
MamI 1891 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI 769 GCATGT [GA]CATG^[CT]
PinAI 236 ACCGGT A^CCGGT
PleI 679 GACTC GAGTC(4/5)
PpuMI 644 AGGTCCT [GA]G^G[AT]CC[CT]
ScaI 1920 AGTACT AGT^ACT
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccII 74 CGCG CG^CG
974 CGCG
AcsI 297 GAATTT [GA]^AATT[CT]
1197 AAATTT
ApoI 297 GAATTT [GA]^AATT[CT]
1197 AAATTT
Asp700I 512 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1998 GAAGCATTTC
AvaI 479 CTCGGG C^[CT]CG[GA]G
681 CTCGGG
BalI 497 TGGCCA TGG^CCA
1607 TGGCCA
BbsI 2170 GAAGAC GAAGAC(2/6)
489 GTCTTC
BpuAI 2170 GAAGAC GAAGAC(2/6)
489 GTCTTC
BsaWI 236 ACCGGT [AT]^CCGG[AT]
777 ACCGGA
BsoBI 479 CTCGGG C^[CT]CG[GA]G
681 CTCGGG
BsrBI 2137 CCGCTC CCGCTC(-3/-3)
40 GAGCGG
BstUI 74 CGCG CG^CG
974 CGCG
EaeI 497 TGGCCA [CT]^GGCC[GA]
1607 TGGCCA
EcoRV 655 GATATC GAT^ATC
1531 GATATC
HaeII 106 AGCGCC [GA]GCGC^[CT]
1489 AGCGCC
HapII 237 CCGG C^CGG
778 CCGG
HpaII 237 CCGG C^CGG
778 CCGG
MluNI 497 TGGCCA TGG^CCA
1607 TGGCCA
MscI 497 TGGCCA TGG^CCA
1607 TGGCCA
MslI 1564 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1869 CATTACCATG
MspA1I 1972 CAGCTG C[AC]G^C[GT]G
2036 CAGCTG
MspI 237 CCGG C^CGG
778 CCGG
MvnI 74 CGCG CG^CG
974 CGCG
PflMI 363 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1756 CCATTCCGTGG
PvuII 1972 CAGCTG CAG^CTG
2036 CAGCTG
SacI 1035 GAGCTC GAGCT^C
1864 GAGCTC
Van91I 363 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1756 CCATTCCGTGG
XcmI 1874 CCATGATGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2113 CCAGGAATGTATTGG
XmnI 512 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1998 GAAGCATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BanII BbvI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HgaI HhaI
HinP1I HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I