(Circular) (Six-base) MAPSORT of: pFN21AA0369.seq Check: 9283 from: 1 to: 6892 pFN21AA0369 6892 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3713 3808 3713 Size: 95 6797 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4702 278 Size: 53 83 186 936 3166 2468 Fragments arranged by size: 3166 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3624 4235 1937 Size: 1687 611 4594 Fragments arranged by size: 4594 1687 611 Acc65I G'GTAC_C Cuts at: 4213 4213 Size: 6892 AclI AA'CG_TT Cuts at: 1563 5019 5392 1563 Size: 3456 373 3063 Fragments arranged by size: 3456 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2508 2723 3611 4955 6339 6607 1632 Size: 876 215 888 1344 1384 268 1917 Fragments arranged by size: 1917 1384 1344 888 876 268 215 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6695 AflIII A'CryG_T Cuts at: 1203 2709 5791 6768 1203 Size: 1506 3082 977 1327 Fragments arranged by size: 3082 1506 1327 977 AgeI A'CCGG_T Cuts at: 2241 5723 2241 Size: 3482 3410 AhdI GACnn_n'nnGTC Cuts at: 5621 5621 Size: 6892 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6892 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6892 AlwNI CAG_nnn'CTG Cuts at: 3689 3989 4043 4635 6207 3689 Size: 300 54 592 1572 4374 Fragments arranged by size: 4374 1572 592 300 54 ApaLI G'TGCA_C Cuts at: 4949 6105 4949 Size: 1156 5736 ApoI r'AATT_y Cuts at: 1493 2302 3202 4201 4427 4579 6688 1493 Size: 809 900 999 226 152 2109 1697 Fragments arranged by size: 2109 1697 999 900 809 226 152 AseI AT'TA_AT Cuts at: 160 1032 5446 160 Size: 872 4414 1606 Fragments arranged by size: 4414 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6892 AvaI C'yCGr_G Cuts at: 1870 1943 2484 2686 4217 1870 Size: 73 541 202 1531 4545 Fragments arranged by size: 4545 1531 541 202 73 BamHI G'GATC_C Cuts at: 4222 4589 4222 Size: 367 6525 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3650 4213 5668 618 Size: 325 217 627 1863 563 1455 1842 Fragments arranged by size: 1863 1842 1455 627 563 325 217 BanII G_rGCy'C Cuts at: 1391 2102 2346 2412 2900 3044 3873 4211 1391 Size: 711 244 66 488 144 829 338 4072 Fragments arranged by size: 4072 829 711 488 338 244 144 66 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2487 4182 928 Size: 971 588 1695 3638 Fragments arranged by size: 3638 1695 971 588 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5083 5117 1903 Size: 34 3146 34 3678 Fragments arranged by size: 3678 3146 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3686 4784 5994 3686 Size: 1098 1210 4584 Fragments arranged by size: 4584 1210 1098 BclI T'GATC_A Cuts at: 1548 3799 1548 Size: 2251 4641 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2267 5503 136 Size: 107 122 71 631 605 595 3236 1525 Fragments arranged by size: 3236 1525 631 605 595 122 107 71 BglII A'GATC_T Cuts at: 6887 6887 Size: 6892 BlpI GC'TnA_GC Cuts at: 2153 4309 2153 Size: 2156 4736 Bme1580I G_kGCm'C Cuts at: 1790 4953 6109 1790 Size: 3163 1156 2573 Fragments arranged by size: 3163 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6892 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3850 4623 5581 Size: 416 53 83 399 24 2433 773 958 Cuts at: 5581 442 Size: 1753 Fragments arranged by size: 2433 1753 958 773 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6892 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3060 3060 Size: 6892 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2444 3138 3654 4065 5552 1239 Size: 812 393 694 516 411 1487 2579 Fragments arranged by size: 2579 1487 812 694 516 411 393 Bpu10I CC'TnA_GC Cuts at: 2983 2983 Size: 6892 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2330 3686 3950 5017 5882 6180 2330 Size: 1356 264 1067 865 298 3042 Fragments arranged by size: 3042 1356 1067 865 298 264 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3411 5555 882 Size: 617 1912 2144 2219 Fragments arranged by size: 2219 2144 1912 617 BsaAI yAC'GTr Cuts at: 493 1204 3664 4683 493 Size: 711 2460 1019 2702 Fragments arranged by size: 2702 2460 1019 711 BsaBI GATnn'nnATC Cuts at: 3900 6886 3900 Size: 2986 3906 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3465 4699 5081 5716 Size: 53 83 186 936 1932 1234 382 635 Cuts at: 5716 275 Size: 1451 Fragments arranged by size: 1932 1451 1234 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2241 2782 5325 5723 5997 6144 6555 2241 Size: 541 2543 398 274 147 411 2578 Fragments arranged by size: 2578 2543 541 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3111 3141 4038 4068 1178 Size: 30 165 30 1708 30 897 30 4002 Fragments arranged by size: 4002 1708 897 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3049 3052 3377 1186 Size: 1863 3 325 4701 Fragments arranged by size: 4701 1863 325 3 BseYI C'CCAG_C Cuts at: 3595 6095 6591 3595 Size: 2500 496 3896 Fragments arranged by size: 3896 2500 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6319 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4342 5103 6131 1540 Size: 275 186 2341 761 1028 2301 Fragments arranged by size: 2341 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2037 2888 3044 3297 3570 3873 4953 5038 6109 Size: 851 156 253 273 303 1080 85 1071 Cuts at: 6109 2037 Size: 2820 Fragments arranged by size: 2820 1080 1071 851 303 273 253 156 85 BsmI GAATG_Cn' Cuts at: 4403 4496 4403 Size: 93 6799 BsmBI CGTCTCn'nnnn_ Cuts at: 3456 3456 Size: 6892 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2102 2346 2412 2888 2900 3044 Size: 399 247 65 244 66 476 12 144 Cuts at: 3044 3297 3570 3873 4211 4953 5038 6109 1391 Size: 253 273 303 338 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 476 399 338 303 273 253 247 244 144 85 66 65 12 BspEI T'CCGG_A Cuts at: 6555 6555 Size: 6892 BspHI T'CATG_A Cuts at: 4781 6511 4781 Size: 1730 5162 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2638 3516 3561 3713 3808 4152 4247 844 Size: 1794 878 45 152 95 344 95 3489 Fragments arranged by size: 3489 1794 878 344 152 95 95 45 BsrBI CCG'CTC Cuts at: 1394 1823 2047 4144 4779 1394 Size: 429 224 2097 635 3507 Fragments arranged by size: 3507 2097 635 429 224 BsrDI GCAATG_nn' Cuts at: 65 1248 3678 5387 5561 65 Size: 1183 2430 1709 174 1396 Fragments arranged by size: 2430 1709 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2241 5536 5723 1700 Size: 110 431 3295 187 2869 Fragments arranged by size: 3295 2869 431 187 110 BsrGI T'GTAC_A Cuts at: 96 2706 3480 3636 96 Size: 2610 774 156 3352 Fragments arranged by size: 3352 2610 774 156 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6892 BssSI C'ACGA_G Cuts at: 4952 5964 4952 Size: 1012 5880 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6892 BstBI TT'CG_AA Cuts at: 5696 5696 Size: 6892 BstEII G'GTnAC_C Cuts at: 1184 2566 1184 Size: 1382 5510 BstXI CCAn_nnnn'nTGG Cuts at: 2680 2680 Size: 6892 BstYI r'GATC_y Cuts at: 1972 4222 4589 4974 4991 6443 6529 6541 6887 Size: 2250 367 385 17 1452 86 12 346 Cuts at: 6887 1972 Size: 1977 Fragments arranged by size: 2250 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2594 3766 4069 513 Size: 552 1529 1172 303 3336 Fragments arranged by size: 3336 1529 1172 552 303 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6153 Fragments arranged by size: 6153 683 56 BtsI GCAGTG_nn' Cuts at: 2116 2496 2505 4408 5202 5222 2116 Size: 380 9 1903 794 20 3786 Fragments arranged by size: 3786 1903 794 380 20 9 ClaI AT'CG_AT Cuts at: 1554 2203 1554 Size: 649 6243 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3645 3680 4128 4163 529 Size: 35 3081 35 448 35 3258 Fragments arranged by size: 3258 3081 448 35 35 35 DraI TTT'AAA Cuts at: 4196 4196 Size: 6892 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5899 809 Size: 507 4583 1802 Fragments arranged by size: 4583 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2502 3612 4339 Size: 54 1425 50 275 18 672 1110 727 Cuts at: 4339 5228 6857 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1110 889 727 672 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4339 1537 Size: 275 2527 4090 Fragments arranged by size: 4090 2527 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3322 4822 1353 Size: 1969 1500 3423 Fragments arranged by size: 3423 1969 1500 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5479 5853 5999 387 Size: 1064 138 3890 374 146 1280 Fragments arranged by size: 3890 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3042 3871 3042 Size: 829 6063 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2444 2508 2723 3138 3611 3654 Size: 393 419 393 64 215 415 473 43 Cuts at: 3654 4065 4955 5552 6339 6607 1239 Size: 411 890 597 787 268 1524 Fragments arranged by size: 1524 890 787 597 473 419 415 411 393 393 268 215 64 43 EcoO109I rG'GnC_Cy Cuts at: 2260 2650 4019 2260 Size: 390 1369 5133 Fragments arranged by size: 5133 1369 390 EcoRI G'AATT_C Cuts at: 4201 4579 4201 Size: 378 6514 EcoRV GAT'ATC Cuts at: 2662 3538 2662 Size: 876 6016 FspI TGC'GCA Cuts at: 5398 5398 Size: 6892 HaeII r_GCGC'y Cuts at: 1678 2115 3498 6039 1678 Size: 437 1383 2541 2531 Fragments arranged by size: 2541 2531 1383 437 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2269 2301 3162 3194 3684 Size: 32 135 32 697 32 861 32 490 Cuts at: 3684 3716 4038 4070 4128 4160 5533 5565 5607 Size: 32 322 32 58 32 1373 32 42 Cuts at: 5607 5639 1373 Size: 32 2626 Fragments arranged by size: 2626 1373 861 697 490 322 135 58 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4236 4482 669 Size: 988 281 2298 246 3079 Fragments arranged by size: 3079 2298 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6892 HpaI GTT'AAC Cuts at: 4482 4482 Size: 6892 KpnI G_GTAC'C Cuts at: 4217 4217 Size: 6892 MfeI C'AATT_G Cuts at: 4491 4491 Size: 6892 MluI A'CGCG_T Cuts at: 6768 6768 Size: 6892 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2271 2694 3111 3160 4194 6006 6190 1305 Size: 966 423 417 49 1034 1812 184 2007 Fragments arranged by size: 2007 1812 1034 966 423 417 184 49 MscI TGG'CCA Cuts at: 10 64 1489 1832 2504 3614 10 Size: 54 1425 343 672 1110 3288 Fragments arranged by size: 3288 1425 1110 672 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3573 3878 4850 5209 5368 518 Size: 648 2407 305 972 359 159 2042 Fragments arranged by size: 2407 2042 972 648 359 305 159 MspA1I CmG'CkG Cuts at: 1606 3979 4043 4308 4985 6133 6378 1606 Size: 2373 64 265 677 1148 245 2120 Fragments arranged by size: 2373 2120 1148 677 265 245 64 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6340 NdeI CA'TA_TG Cuts at: 387 387 Size: 6892 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6892 NotI GC'GGCC_GC Cuts at: 4339 4339 Size: 6892 NspI r_CATG'y Cuts at: 1132 1229 2778 4250 5795 1132 Size: 97 1549 1472 1545 2229 Fragments arranged by size: 2229 1549 1545 1472 97 PasI CC'CwG_GG Cuts at: 1348 2311 3939 1348 Size: 963 1628 4301 Fragments arranged by size: 4301 1628 963 PciI A'CATG_T Cuts at: 5791 5791 Size: 6892 PflMI CCAn_nnn'nTGG Cuts at: 1108 2374 3767 1108 Size: 1266 1393 4233 Fragments arranged by size: 4233 1393 1266 PmeI GTTT'AAAC Cuts at: 4196 4196 Size: 6892 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4961 4993 6474 6506 4961 Size: 32 1481 32 5347 Fragments arranged by size: 5347 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2650 2650 Size: 6892 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6892 PsiI TTA'TAA Cuts at: 4462 4462 Size: 6892 PstI C_TGCA'G Cuts at: 830 2123 2148 3092 4244 830 Size: 1293 25 944 1152 3478 Fragments arranged by size: 3478 1293 1152 944 25 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6892 PvuII CAG'CTG Cuts at: 3979 4043 3979 Size: 64 6828 SacI G_AGCT'C Cuts at: 3044 3873 3044 Size: 829 6063 SalI G'TCGA_C Cuts at: 1936 4234 1936 Size: 2298 4594 SbfI CC_TGCA'GG Cuts at: 4244 4244 Size: 6892 ScaI AGT'ACT Cuts at: 3927 5140 6852 3927 Size: 1213 1712 3967 Fragments arranged by size: 3967 1712 1213 SfcI C'TryA_G Cuts at: 826 1045 2119 2144 3088 4240 5375 6056 6247 Size: 219 1074 25 944 1152 1135 681 191 Cuts at: 6247 826 Size: 1471 Fragments arranged by size: 1471 1152 1135 1074 944 681 219 191 25 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6892 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6892 SmaI CCC'GGG Cuts at: 1872 4219 1872 Size: 2347 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2345 3701 3929 4996 5897 6159 Size: 197 926 402 1356 228 1067 901 262 Cuts at: 6159 820 Size: 1553 Fragments arranged by size: 1553 1356 1067 926 901 402 262 228 197 SnaBI TAC'GTA Cuts at: 493 4683 493 Size: 4190 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6892 SphI G_CATG'C Cuts at: 1132 4250 1132 Size: 3118 3774 SspI AAT'ATT Cuts at: 5 52 4816 5 Size: 47 4764 2081 Fragments arranged by size: 4764 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6892 StyI C'CwwG_G Cuts at: 513 1065 4331 513 Size: 552 3266 3074 Fragments arranged by size: 3266 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3022 3587 4919 4936 5089 5274 5719 Size: 1255 1442 565 1332 17 153 185 445 Cuts at: 5719 325 Size: 1498 Fragments arranged by size: 1498 1442 1332 1255 565 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2427 2706 3480 Size: 274 80 33 51 1443 450 279 774 Cuts at: 3480 3636 3925 5138 6850 96 Size: 156 289 1213 1712 138 Fragments arranged by size: 1712 1443 1213 774 450 289 279 274 156 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3111 3143 6852 6884 3111 Size: 32 3709 32 3119 Fragments arranged by size: 3709 3119 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6892 XbaI T'CTAG_A Cuts at: 4228 4228 Size: 6892 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3886 4125 3886 Size: 239 6653 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6892 XmaI C'CCGG_G Cuts at: 1870 4217 1870 Size: 2347 4545 XmnI GAAnn'nnTTC Cuts at: 2521 4007 5021 2521 Size: 1486 1014 4392 Fragments arranged by size: 4392 1486 1014 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4700 276 Size: 53 83 186 936 3166 2468 Fragments arranged by size: 3166 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI