Restriction map for
pFN21AA0369
(Linear) (Six-base) MAPSORT of: KIAA0369.seq Check: 2843 from: 1 to: 2187
KIAA0369.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1708 1803 2187
Size: 1708 95 384
Fragments arranged by size:
1708 384 95
AccI GT'mk_AC
Cuts at: 0 1619 2187
Size: 1619 568
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 718 1606 2187
Size: 503 215 888 581
Fragments arranged by size:
888 581 503 215
AflIII A'CryG_T
Cuts at: 0 704 2187
Size: 704 1483
AgeI A'CCGG_T
Cuts at: 0 236 2187
Size: 236 1951
AlwNI CAG_nnn'CTG
Cuts at: 0 1684 1984 2038 2187
Size: 1684 300 54 149
Fragments arranged by size:
1684 300 149 54
ApoI r'AATT_y
Cuts at: 0 297 1197 2187
Size: 297 900 990
Fragments arranged by size:
990 900 297
AvaI C'yCGr_G
Cuts at: 0 479 681 2187
Size: 479 202 1506
Fragments arranged by size:
1506 479 202
BanI G'GyrC_C
Cuts at: 0 1645 2187
Size: 1645 542
BanII G_rGCy'C
Cuts at: 0 97 341 407 895 1039 1868 2187
Size: 97 244 66 488 144 829 319
Fragments arranged by size:
829 488 319 244 144 97 66
BbsI GAAGACnn'nnnn_
Cuts at: 0 482 2177 2187
Size: 482 1695 10
Fragments arranged by size:
1695 482 10
BciVI GTATCCnnnnn_n'
Cuts at: 0 1681 2187
Size: 1681 506
BclI T'GATC_A
Cuts at: 0 1794 2187
Size: 1794 393
BglI GCCn_nnn'nGGC
Cuts at: 0 262 2187
Size: 262 1925
BlpI GC'TnA_GC
Cuts at: 0 148 2187
Size: 148 2039
BmrI ACTGGGnnnn_n'
Cuts at: 0 1845 2187
Size: 1845 342
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1055 2187
Size: 1055 1132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 439 1133 1649 2060 2187
Size: 46 393 694 516 411 127
Fragments arranged by size:
694 516 411 393 127 46
Bpu10I CC'TnA_GC
Cuts at: 0 978 2187
Size: 978 1209
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 1681 1945 2187
Size: 325 1356 264 242
Fragments arranged by size:
1356 325 264 242
BsaI GGTCTCn'nnnn_
Cuts at: 0 1406 2187
Size: 1406 781
BsaAI yAC'GTr
Cuts at: 0 1659 2187
Size: 1659 528
BsaBI GATnn'nnATC
Cuts at: 0 1895 2187
Size: 1895 292
BsaHI Gr'CG_yC
Cuts at: 0 1460 2187
Size: 1460 727
BsaWI w'CCGG_w
Cuts at: 0 236 777 2187
Size: 236 541 1410
Fragments arranged by size:
1410 541 236
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1106 1136 2033 2063 2187
Size: 1106 30 897 30 124
Fragments arranged by size:
1106 897 124 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1044 1047 1372 2187
Size: 1044 3 325 815
Fragments arranged by size:
1044 815 325 3
BseYI C'CCAG_C
Cuts at: 0 1590 2187
Size: 1590 597
BsiHKAI G_wGCw'C
Cuts at: 0 32 883 1039 1292 1565 1868 2187
Size: 32 851 156 253 273 303 319
Fragments arranged by size:
851 319 303 273 253 156 32
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1451 2187
Size: 1451 736
Bsp1286I G_dGCh'C
Cuts at: 0 32 97 341 407 883 895 1039 1292
Size: 32 65 244 66 476 12 144 253
Cuts at: 1292 1565 1868 2187
Size: 273 303 319
Fragments arranged by size:
476 319 303 273 253 244 144 66
65 32 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 633 1511 1556 1708 1803 2147 2187
Size: 633 878 45 152 95 344 40
Fragments arranged by size:
878 633 344 152 95 45 40
BsrBI CCG'CTC
Cuts at: 0 42 2139 2187
Size: 42 2097 48
Fragments arranged by size:
2097 48 42
BsrDI GCAATG_nn'
Cuts at: 0 1673 2187
Size: 1673 514
BsrFI r'CCGG_y
Cuts at: 0 236 2187
Size: 236 1951
BsrGI T'GTAC_A
Cuts at: 0 701 1475 1631 2187
Size: 701 774 156 556
Fragments arranged by size:
774 701 556 156
BstEII G'GTnAC_C
Cuts at: 0 561 2187
Size: 561 1626
BstXI CCAn_nnnn'nTGG
Cuts at: 0 675 2187
Size: 675 1512
BtgI C'CryG_G
Cuts at: 0 589 1761 2064 2187
Size: 589 1172 303 123
Fragments arranged by size:
1172 589 303 123
BtsI GCAGTG_nn'
Cuts at: 0 111 491 500 2187
Size: 111 380 9 1687
Fragments arranged by size:
1687 380 111 9
ClaI AT'CG_AT
Cuts at: 0 198 2187
Size: 198 1989
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1640 1675 2123 2158 2187
Size: 1640 35 448 35 29
Fragments arranged by size:
1640 448 35 35 29
EaeI y'GGCC_r
Cuts at: 0 497 1607 2187
Size: 497 1110 580
Fragments arranged by size:
1110 580 497
EarI CTCTTCn'nnn_
Cuts at: 0 1317 2187
Size: 1317 870
EcoICRI GAG'CTC
Cuts at: 0 1037 1866 2187
Size: 1037 829 321
Fragments arranged by size:
1037 829 321
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 439 503 718 1133 1606 1649 2060
Size: 46 393 64 215 415 473 43 411
Cuts at: 2060 2187
Size: 127
Fragments arranged by size:
473 415 411 393 215 127 64 46
43
EcoO109I rG'GnC_Cy
Cuts at: 0 255 645 2014 2187
Size: 255 390 1369 173
Fragments arranged by size:
1369 390 255 173
EcoRV GAT'ATC
Cuts at: 0 657 1533 2187
Size: 657 876 654
Fragments arranged by size:
876 657 654
HaeII r_GCGC'y
Cuts at: 0 110 1493 2187
Size: 110 1383 694
Fragments arranged by size:
1383 694 110
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 264 296 1157 1189 1679 1711 2033 2065
Size: 264 32 861 32 490 32 322 32
Cuts at: 2065 2123 2155 2187
Size: 58 32 32
Fragments arranged by size:
861 490 322 264 58 32 32 32
32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 689 1106 1155 2187
Size: 266 423 417 49 1032
Fragments arranged by size:
1032 423 417 266 49
MscI TGG'CCA
Cuts at: 0 499 1609 2187
Size: 499 1110 578
Fragments arranged by size:
1110 578 499
MslI CAynn'nnrTG
Cuts at: 0 1568 1873 2187
Size: 1568 305 314
Fragments arranged by size:
1568 314 305
MspA1I CmG'CkG
Cuts at: 0 1974 2038 2187
Size: 1974 64 149
Fragments arranged by size:
1974 149 64
NspI r_CATG'y
Cuts at: 0 773 2187
Size: 773 1414
PasI CC'CwG_GG
Cuts at: 0 306 1934 2187
Size: 306 1628 253
Fragments arranged by size:
1628 306 253
PflMI CCAn_nnn'nTGG
Cuts at: 0 369 1762 2187
Size: 369 1393 425
Fragments arranged by size:
1393 425 369
PpuMI rG'GwC_Cy
Cuts at: 0 645 2187
Size: 645 1542
PstI C_TGCA'G
Cuts at: 0 118 143 1087 2187
Size: 118 25 944 1100
Fragments arranged by size:
1100 944 118 25
PvuII CAG'CTG
Cuts at: 0 1974 2038 2187
Size: 1974 64 149
Fragments arranged by size:
1974 149 64
SacI G_AGCT'C
Cuts at: 0 1039 1868 2187
Size: 1039 829 319
Fragments arranged by size:
1039 829 319
ScaI AGT'ACT
Cuts at: 0 1922 2187
Size: 1922 265
SfcI C'TryA_G
Cuts at: 0 114 139 1083 2187
Size: 114 25 944 1104
Fragments arranged by size:
1104 944 114 25
SmlI C'TyrA_G
Cuts at: 0 340 1696 1924 2187
Size: 340 1356 228 263
Fragments arranged by size:
1356 340 263 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1017 1582 2187
Size: 1017 565 605
Fragments arranged by size:
1017 605 565
TatI w'GTAC_w
Cuts at: 0 422 701 1475 1631 1920 2187
Size: 422 279 774 156 289 267
Fragments arranged by size:
774 422 289 279 267 156
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1106 1138 2187
Size: 1106 32 1049
Fragments arranged by size:
1106 1049 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1881 2120 2187
Size: 1881 239 67
Fragments arranged by size:
1881 239 67
XmnI GAAnn'nnTTC
Cuts at: 0 516 2002 2187
Size: 516 1486 185
Fragments arranged by size:
1486 516 185
Enzymes that do cut:
AarI AccI AcuI AflIII AgeI AlwNI ApoI AvaI
BanI BanII BbsI BciVI BclI BglI BlpI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BstEII BstXI BtgI BtsI
ClaI CspCI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV
HaeII Hin4I MmeI MscI MslI MspA1I NspI PasI
PflMI PpuMI PstI PvuII SacI ScaI SfcI SmlI
TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AhdI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BfrBI BglII Bme1580I BmgBI
BmtI BsgI BsiEI BsiWI BsmI BspEI BspHI BssHII
BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI DraI
DraIII DrdI EagI EciI EcoNI EcoRI FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI Tth111I XbaI XhoI XmaI ZraI