(Circular) (Six-base) MAPSORT of: pFN21AA0363.seq Check: 333 from: 1 to: 9112 pFN21AA0363 9112 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6922 278 Size: 53 83 186 936 5386 2468 Fragments arranged by size: 5386 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6455 1937 Size: 4518 4594 Acc65I G'GTAC_C Cuts at: 5423 6433 5423 Size: 1010 8102 AclI AA'CG_TT Cuts at: 1563 7239 7612 1563 Size: 5676 373 3063 Fragments arranged by size: 5676 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2472 3636 3756 3876 3996 4116 4236 4356 Size: 840 1164 120 120 120 120 120 120 Cuts at: 4356 4476 4997 5175 7175 8559 8827 1632 Size: 120 521 178 2000 1384 268 1917 Fragments arranged by size: 2000 1917 1384 1164 840 521 268 178 120 120 120 120 120 120 120 AfeI AGC'GCT Cuts at: 2139 2139 Size: 9112 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8915 AflIII A'CryG_T Cuts at: 1203 8011 8988 1203 Size: 6808 977 1327 Fragments arranged by size: 6808 1327 977 AgeI A'CCGG_T Cuts at: 7943 7943 Size: 9112 AhdI GACnn_n'nnGTC Cuts at: 2794 7841 2794 Size: 5047 4065 AleI CACnn'nnGTG Cuts at: 1166 3599 3719 3839 3959 4079 4199 4319 4439 Size: 2433 120 120 120 120 120 120 120 Cuts at: 4439 4905 5384 6107 1166 Size: 466 479 723 4171 Fragments arranged by size: 4171 2433 723 479 466 120 120 120 120 120 120 120 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5354 1184 Size: 4170 4942 AlwNI CAG_nnn'CTG Cuts at: 2038 2234 2330 2818 4604 4661 4751 5223 5596 Size: 196 96 488 1786 57 90 472 373 Cuts at: 5596 6855 8427 2038 Size: 1259 1572 2723 Fragments arranged by size: 2723 1786 1572 1259 488 472 373 196 96 90 57 ApaI G_GGCC'C Cuts at: 2304 5405 5727 5812 2304 Size: 3101 322 85 5604 Fragments arranged by size: 5604 3101 322 85 ApaLI G'TGCA_C Cuts at: 6152 7169 8325 6152 Size: 1017 1156 6939 Fragments arranged by size: 6939 1156 1017 ApoI r'AATT_y Cuts at: 1493 6421 6647 6799 8908 1493 Size: 4928 226 152 2109 1697 Fragments arranged by size: 4928 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7666 160 Size: 872 6634 1606 Fragments arranged by size: 6634 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9112 AvaI C'yCGr_G Cuts at: 1870 1943 2183 2374 5762 5805 5818 6228 6437 Size: 73 240 191 3388 43 13 410 209 Cuts at: 6437 1870 Size: 4545 Fragments arranged by size: 4545 3388 410 240 209 191 73 43 13 BamHI G'GATC_C Cuts at: 2746 6442 6809 2746 Size: 3696 367 5049 Fragments arranged by size: 5049 3696 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5330 5423 6181 6433 7888 Size: 325 217 627 3543 93 758 252 1455 Cuts at: 7888 618 Size: 1842 Fragments arranged by size: 3543 1842 1455 758 627 325 252 217 93 BanII G_rGCy'C Cuts at: 1391 2076 2304 2381 2544 2952 5090 5136 5405 Size: 685 228 77 163 408 2138 46 269 Cuts at: 5405 5460 5517 5727 5735 5754 5812 5819 6092 Size: 55 57 210 8 19 58 7 273 Cuts at: 6092 6195 6361 6431 1391 Size: 103 166 70 4072 Fragments arranged by size: 4072 2138 685 408 273 269 228 210 166 163 103 77 70 58 57 55 46 19 8 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2019 2996 5181 5499 5511 5802 6073 Size: 971 120 977 2185 318 12 291 271 Cuts at: 6073 928 Size: 3967 Fragments arranged by size: 3967 2185 977 971 318 291 271 120 12 BbvCI CC'TCA_GC Cuts at: 2432 3300 5146 5642 6196 2432 Size: 868 1846 496 554 5348 Fragments arranged by size: 5348 1846 868 554 496 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2840 2861 2874 2895 7303 Size: 34 50 34 819 21 13 21 4408 Cuts at: 7303 7337 1903 Size: 34 3678 Fragments arranged by size: 4408 3678 819 50 34 34 34 21 21 13 BciVI GTATCCnnnnn_n' Cuts at: 7004 8214 7004 Size: 1210 7902 BclI T'GATC_A Cuts at: 1548 1548 Size: 9112 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2598 3062 4616 Size: 107 122 71 631 605 926 464 1554 Cuts at: 4616 5115 5162 5739 5981 6356 7723 136 Size: 499 47 577 242 375 1367 1525 Fragments arranged by size: 1554 1525 1367 926 631 605 577 499 464 375 242 122 107 71 47 BglII A'GATC_T Cuts at: 3327 3559 3799 3919 4039 4159 4279 4399 5688 Size: 232 240 120 120 120 120 120 1289 Cuts at: 5688 9107 3327 Size: 3419 3332 Fragments arranged by size: 3419 3332 1289 240 232 120 120 120 120 120 BlpI GC'TnA_GC Cuts at: 2331 4620 4917 6529 2331 Size: 2289 297 1612 4914 Fragments arranged by size: 4914 2289 1612 297 Bme1580I G_kGCm'C Cuts at: 1790 2163 2304 4591 5405 5727 5812 6156 6186 Size: 373 141 2287 814 322 85 344 30 Cuts at: 6186 7173 8329 1790 Size: 987 1156 2573 Fragments arranged by size: 2573 2287 1156 987 814 373 344 322 141 85 30 BmgBI CAC'GTC Cuts at: 1318 2100 2796 5247 1318 Size: 782 696 2451 5183 Fragments arranged by size: 5183 2451 782 696 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5291 5313 5613 Size: 416 53 83 399 24 3874 22 300 Cuts at: 5613 6843 7801 442 Size: 1230 958 1753 Fragments arranged by size: 3874 1753 1230 958 416 399 300 83 53 24 22 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9112 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4843 5636 5815 4843 Size: 793 179 8140 Fragments arranged by size: 8140 793 179 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 2957 3191 4967 5474 5810 6260 7772 Size: 851 867 234 1776 507 336 450 1512 Cuts at: 7772 1239 Size: 2579 Fragments arranged by size: 2579 1776 1512 867 851 507 450 336 234 Bpu10I CC'TnA_GC Cuts at: 2141 2432 3033 3300 4818 5084 5146 5642 6196 Size: 291 601 267 1518 266 62 496 554 Cuts at: 6196 2141 Size: 5057 Fragments arranged by size: 5057 1518 601 554 496 291 267 266 62 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5730 7237 8102 8400 5730 Size: 1507 865 298 6442 Fragments arranged by size: 6442 1507 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4934 5927 7775 882 Size: 617 3435 993 1848 2219 Fragments arranged by size: 3435 2219 1848 993 617 BsaAI yAC'GTr Cuts at: 493 1204 6903 493 Size: 711 5699 2702 Fragments arranged by size: 5699 2702 711 BsaBI GATnn'nnATC Cuts at: 6032 9106 6032 Size: 3074 6038 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2313 6919 7301 7936 Size: 53 83 186 936 780 4606 382 635 Cuts at: 7936 275 Size: 1451 Fragments arranged by size: 4606 1451 936 780 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7545 7943 8217 8364 8775 7545 Size: 398 274 147 411 7882 Fragments arranged by size: 7882 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2035 2065 2564 2594 2891 Size: 30 165 30 632 30 499 30 297 Cuts at: 2891 2921 1178 Size: 30 7369 Fragments arranged by size: 7369 632 499 297 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2000 2043 2108 2572 2747 2798 2878 3011 Size: 814 43 65 464 175 51 80 133 Cuts at: 3011 3014 3060 3399 4679 5525 5630 5703 5874 Size: 3 46 339 1280 846 105 73 171 Cuts at: 5874 5945 6275 6278 6281 1186 Size: 71 330 3 3 4017 Fragments arranged by size: 4017 1280 846 814 464 339 330 175 171 133 105 80 73 71 65 51 46 43 3 3 3 BseYI C'CCAG_C Cuts at: 2406 2475 2524 2771 3242 3606 3726 3846 3966 Size: 69 49 247 471 364 120 120 120 Cuts at: 3966 4086 4206 4326 4446 4751 4835 5089 5262 Size: 120 120 120 120 305 84 254 173 Cuts at: 5262 5375 5432 5461 5734 5914 5995 6091 6144 Size: 113 57 29 273 180 81 96 53 Cuts at: 6144 8315 8811 2406 Size: 2171 496 2707 Fragments arranged by size: 2707 2171 496 471 364 305 273 254 247 180 173 120 120 120 120 120 120 120 113 96 84 81 69 57 53 49 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2504 5235 5915 1163 Size: 573 768 2731 680 4360 Fragments arranged by size: 4360 2731 768 680 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2394 6562 7323 8351 1540 Size: 275 186 393 4168 761 1028 2301 Fragments arranged by size: 4168 2301 1028 761 393 275 186 BsiHKAI G_wGCw'C Cuts at: 6156 7173 7258 8329 6156 Size: 1017 85 1071 6939 Fragments arranged by size: 6939 1071 1017 85 BsmI GAATG_Cn' Cuts at: 4678 5827 6623 6716 4678 Size: 1149 796 93 7074 Fragments arranged by size: 7074 1149 796 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2090 2090 Size: 9112 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2163 2304 2381 2544 2952 4591 Size: 399 286 87 141 77 163 408 1639 Cuts at: 4591 5090 5136 5405 5460 5517 5727 5735 5754 Size: 499 46 269 55 57 210 8 19 Cuts at: 5754 5812 5819 6092 6156 6186 6195 6361 6431 Size: 58 7 273 64 30 9 166 70 Cuts at: 6431 7173 7258 8329 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1639 1071 742 499 408 399 286 273 269 210 166 163 141 87 85 77 70 64 58 57 55 46 30 19 9 8 7 BspEI T'CCGG_A Cuts at: 8775 8775 Size: 9112 BspHI T'CATG_A Cuts at: 7001 8731 7001 Size: 1730 7382 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3181 6467 844 Size: 2337 3286 3489 Fragments arranged by size: 3489 3286 2337 BsrBI CCG'CTC Cuts at: 1394 1823 3006 6999 1394 Size: 429 1183 3993 3507 Fragments arranged by size: 3993 3507 1183 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3300 5991 7607 7781 65 Size: 1183 2052 2691 1616 174 1396 Fragments arranged by size: 2691 2052 1616 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4822 7756 7943 1700 Size: 110 3012 2934 187 2869 Fragments arranged by size: 3012 2934 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2937 96 Size: 2841 6271 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9112 BssSI C'ACGA_G Cuts at: 7172 8184 7172 Size: 1012 8100 BstAPI GCAn_nnn'nTGC Cuts at: 794 4586 5692 794 Size: 3792 1106 4214 Fragments arranged by size: 4214 3792 1106 BstBI TT'CG_AA Cuts at: 7916 7916 Size: 9112 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9112 BstXI CCAn_nnnn'nTGG Cuts at: 2156 5677 2156 Size: 3521 5591 BstYI r'GATC_y Cuts at: 1972 2746 3327 3559 3799 3919 4039 4159 4279 Size: 774 581 232 240 120 120 120 120 Cuts at: 4279 4399 5688 6442 6809 7194 7211 8663 8749 Size: 120 1289 754 367 385 17 1452 86 Cuts at: 8749 8761 9107 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1289 774 754 581 385 367 346 240 232 120 120 120 120 120 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2016 2470 4528 4642 4662 5061 2016 Size: 454 2058 114 20 399 6067 Fragments arranged by size: 6067 2058 454 399 114 20 BtgI C'CryG_G Cuts at: 513 1065 2043 2305 2499 3057 3288 4614 5385 Size: 552 978 262 194 558 231 1326 771 Cuts at: 5385 5661 5839 6408 513 Size: 276 178 569 3217 Fragments arranged by size: 3217 1326 978 771 569 558 552 276 262 231 194 178 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5923 6049 487 Size: 683 56 4697 126 3550 Fragments arranged by size: 4697 3550 683 126 56 BtsI GCAGTG_nn' Cuts at: 2700 3162 3348 5299 5903 5932 5968 6628 7422 Size: 462 186 1951 604 29 36 660 794 Cuts at: 7422 7442 2700 Size: 20 4370 Fragments arranged by size: 4370 1951 794 660 604 462 186 36 29 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9112 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3564 3599 3684 3719 3804 3839 3924 Size: 35 3000 35 85 35 85 35 85 Cuts at: 3924 3959 4044 4079 4164 4199 4284 4319 4404 Size: 35 85 35 85 35 85 35 85 Cuts at: 4404 4439 529 Size: 35 5202 Fragments arranged by size: 5202 3000 85 85 85 85 85 85 85 35 35 35 35 35 35 35 35 35 DraI TTT'AAA Cuts at: 6416 6416 Size: 9112 DraIII CAC_nnn'GTG Cuts at: 3396 3396 Size: 9112 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8119 809 Size: 507 6803 1802 Fragments arranged by size: 6803 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 2391 2625 Size: 54 1425 50 275 18 231 330 234 Cuts at: 2625 4551 4608 5154 6559 7448 9077 8 Size: 1926 57 546 1405 889 1629 43 Fragments arranged by size: 1926 1629 1425 1405 889 546 330 275 234 231 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2391 6559 1537 Size: 275 579 4168 4090 Fragments arranged by size: 4168 4090 579 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2419 2724 3129 3612 3732 3852 3972 4092 Size: 1066 305 405 483 120 120 120 120 Cuts at: 4092 4212 4332 4452 6246 6252 6267 7042 1353 Size: 120 120 120 1794 6 15 775 3423 Fragments arranged by size: 3423 1794 1066 775 483 405 305 120 120 120 120 120 120 120 15 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2759 7699 8073 8219 387 Size: 1064 138 1170 4940 374 146 1280 Fragments arranged by size: 4940 1280 1170 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2472 2957 3191 3636 3756 3876 Size: 393 458 382 485 234 445 120 120 Cuts at: 3876 3996 4116 4236 4356 4476 4967 4997 5175 Size: 120 120 120 120 120 491 30 178 Cuts at: 5175 5474 5810 6260 7175 7772 8559 8827 1239 Size: 299 336 450 915 597 787 268 1524 Fragments arranged by size: 1524 915 787 597 491 485 458 450 445 393 382 336 299 268 234 178 120 120 120 120 120 120 120 30 EcoNI CCTnn'n_nnAGG Cuts at: 2015 5363 5477 2015 Size: 3348 114 5650 Fragments arranged by size: 5650 3348 114 EcoO109I rG'GnC_Cy Cuts at: 2020 2107 2301 2479 2532 2764 3090 3139 3502 Size: 87 194 178 53 232 326 49 363 Cuts at: 3502 3622 3742 3862 3982 4102 4222 4342 4462 Size: 120 120 120 120 120 120 120 120 Cuts at: 4462 4520 4646 4804 5032 5394 5402 5482 5724 Size: 58 126 158 228 362 8 80 242 Cuts at: 5724 5809 5911 6136 6225 2020 Size: 85 102 225 89 4907 Fragments arranged by size: 4907 363 362 326 242 232 228 225 194 178 158 126 120 120 120 120 120 120 120 120 102 89 87 85 80 58 53 49 8 EcoRI G'AATT_C Cuts at: 6421 6799 6421 Size: 378 8734 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3158 6016 3158 Size: 2858 6254 FspI TGC'GCA Cuts at: 7618 7618 Size: 9112 HaeII r_GCGC'y Cuts at: 1678 2141 8259 1678 Size: 463 6118 2531 Fragments arranged by size: 6118 2531 463 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2009 2041 2453 2485 2531 Size: 32 135 32 437 32 412 32 46 Cuts at: 2531 2563 2564 2596 2780 2812 2861 2885 2891 Size: 32 1 32 184 32 49 24 6 Cuts at: 2891 2893 2917 2923 3431 3463 3523 3551 3555 Size: 2 24 6 508 32 60 28 4 Cuts at: 3555 3583 3643 3675 3763 3791 3795 3823 3883 Size: 28 60 32 88 28 4 28 60 Cuts at: 3883 3911 3915 3943 4003 4031 4035 4063 4123 Size: 28 4 28 60 28 4 28 60 Cuts at: 4123 4151 4155 4183 4243 4271 4275 4303 4363 Size: 28 4 28 60 28 4 28 60 Cuts at: 4363 4391 4395 4423 4483 4515 6125 6157 7753 Size: 28 4 28 60 32 1610 32 1596 Cuts at: 7753 7785 7827 7859 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1610 1596 508 437 412 184 135 88 60 60 60 60 60 60 60 60 49 46 42 32 32 32 32 32 32 32 32 32 32 32 32 32 28 28 28 28 28 28 28 28 28 28 28 28 28 28 24 24 6 6 4 4 4 4 4 4 4 2 1 HincII GTy'rAC Cuts at: 669 1657 1938 6388 6456 6702 669 Size: 988 281 4450 68 246 3079 Fragments arranged by size: 4450 3079 988 281 246 68 HindIII A'AGCT_T Cuts at: 748 748 Size: 9112 HpaI GTT'AAC Cuts at: 6702 6702 Size: 9112 KpnI G_GTAC'C Cuts at: 5427 6437 5427 Size: 1010 8102 MfeI C'AATT_G Cuts at: 6711 6711 Size: 9112 MluI A'CGCG_T Cuts at: 8988 8988 Size: 9112 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3389 4744 8226 8410 1305 Size: 2084 1355 3482 184 2007 Fragments arranged by size: 3482 2084 2007 1355 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2627 4553 4610 5156 10 Size: 54 1425 343 795 1926 57 546 3966 Fragments arranged by size: 3966 1926 1425 795 546 343 57 54 MslI CAynn'nnrTG Cuts at: 518 1166 3293 3599 3719 3839 3959 4079 4199 Size: 648 2127 306 120 120 120 120 120 Cuts at: 4199 4319 4439 4905 5384 6107 7070 7429 7588 Size: 120 120 466 479 723 963 359 159 Cuts at: 7588 518 Size: 2042 Fragments arranged by size: 2127 2042 963 723 648 479 466 359 306 159 120 120 120 120 120 120 120 MspA1I CmG'CkG Cuts at: 1606 2406 2943 3269 4742 5150 5567 5738 6167 Size: 800 537 326 1473 408 417 171 429 Cuts at: 6167 6528 7205 8353 8598 1606 Size: 361 677 1148 245 2120 Fragments arranged by size: 2120 1473 1148 800 677 537 429 417 408 361 326 245 171 NaeI GCC'GGC Cuts at: 4824 4824 Size: 9112 NcoI C'CATG_G Cuts at: 513 1065 2499 3288 4614 5661 6408 513 Size: 552 1434 789 1326 1047 747 3217 Fragments arranged by size: 3217 1434 1326 1047 789 747 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9112 NgoMIV G'CCGG_C Cuts at: 4822 4822 Size: 9112 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9112 NotI GC'GGCC_GC Cuts at: 6559 6559 Size: 9112 NspI r_CATG'y Cuts at: 1132 1229 4884 6470 8015 1132 Size: 97 3655 1586 1545 2229 Fragments arranged by size: 3655 2229 1586 1545 97 PasI CC'CwG_GG Cuts at: 1348 4592 5398 5812 1348 Size: 3244 806 414 4648 Fragments arranged by size: 4648 3244 806 414 PciI A'CATG_T Cuts at: 8011 8011 Size: 9112 PflMI CCAn_nnn'nTGG Cuts at: 1108 3242 1108 Size: 2134 6978 PfoI T'CCnGG_A Cuts at: 2865 2973 4692 2865 Size: 108 1719 7285 Fragments arranged by size: 7285 1719 108 PmeI GTTT'AAAC Cuts at: 6416 6416 Size: 9112 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7181 7213 8694 8726 7181 Size: 32 1481 32 7567 Fragments arranged by size: 7567 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2532 2764 3502 5032 5482 6136 2532 Size: 232 738 1530 450 654 5508 Fragments arranged by size: 5508 1530 738 654 450 232 PshAI GACnn'nnGTC Cuts at: 1613 5005 1613 Size: 3392 5720 PsiI TTA'TAA Cuts at: 6682 6682 Size: 9112 PspOMI G'GGCC_C Cuts at: 2300 5401 5723 5808 2300 Size: 3101 322 85 5604 Fragments arranged by size: 5604 3101 322 85 PstI C_TGCA'G Cuts at: 830 2572 2814 2884 3030 3192 3355 3472 3592 Size: 1742 242 70 146 162 163 117 120 Cuts at: 3592 3712 3832 3952 4072 4192 4312 4432 4705 Size: 120 120 120 120 120 120 120 273 Cuts at: 4705 4747 5346 6464 830 Size: 42 599 1118 3478 Fragments arranged by size: 3478 1742 1118 599 273 242 163 162 146 120 120 120 120 120 120 120 120 117 70 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9112 PvuII CAG'CTG Cuts at: 2406 4742 5150 5738 6167 2406 Size: 2336 408 588 429 5351 Fragments arranged by size: 5351 2336 588 429 408 SalI G'TCGA_C Cuts at: 1936 6454 1936 Size: 4518 4594 SanDI GG'GwC_CC Cuts at: 2532 2532 Size: 9112 SbfI CC_TGCA'GG Cuts at: 6464 6464 Size: 9112 ScaI AGT'ACT Cuts at: 7360 9072 7360 Size: 1712 7400 SexAI A'CCwGG_T Cuts at: 4713 4713 Size: 9112 SfcI C'TryA_G Cuts at: 826 1045 2568 2810 2880 3026 3188 3351 3462 Size: 219 1523 242 70 146 162 163 111 Cuts at: 3462 3468 3588 3708 3828 3948 4068 4188 4308 Size: 6 120 120 120 120 120 120 120 Cuts at: 4308 4428 4686 4701 4743 5342 6460 7595 8276 Size: 120 258 15 42 599 1118 1135 681 Cuts at: 8276 8467 826 Size: 191 1471 Fragments arranged by size: 1523 1471 1135 1118 681 599 258 242 219 191 163 162 146 120 120 120 120 120 120 120 120 111 70 42 15 6 SfiI GGCCn_nnn'nGGCC Cuts at: 2598 5162 2598 Size: 2564 6548 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9112 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9112 SmaI CCC'GGG Cuts at: 1872 6230 6439 1872 Size: 4358 209 4545 Fragments arranged by size: 4545 4358 209 SmlI C'TyrA_G Cuts at: 820 1017 1943 5745 7216 8117 8379 820 Size: 197 926 3802 1471 901 262 1553 Fragments arranged by size: 3802 1553 1471 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6903 493 Size: 6410 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9112 SphI G_CATG'C Cuts at: 1132 6470 1132 Size: 5338 3774 SspI AAT'ATT Cuts at: 5 52 7036 5 Size: 47 6984 2081 Fragments arranged by size: 6984 2081 47 StuI AGG'CCT Cuts at: 1517 3204 4640 4867 4974 5109 1517 Size: 1687 1436 227 107 135 5520 Fragments arranged by size: 5520 1687 1436 227 135 107 StyI C'CwwG_G Cuts at: 513 1065 2499 3018 3288 4614 4707 4760 5326 Size: 552 1434 519 270 1326 93 53 566 Cuts at: 5326 5364 5649 5661 6201 6345 6408 6551 513 Size: 38 285 12 540 144 63 143 3074 Fragments arranged by size: 3074 1434 1326 566 552 540 519 285 270 144 143 93 63 53 38 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2134 2538 6235 7139 7156 7309 7494 Size: 1255 554 404 3697 904 17 153 185 Cuts at: 7494 7939 325 Size: 445 1498 Fragments arranged by size: 3697 1498 1255 904 554 445 404 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2937 5278 7358 Size: 274 80 33 51 1443 960 2341 2080 Cuts at: 7358 9070 96 Size: 1712 138 Fragments arranged by size: 2341 2080 1712 1443 960 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2035 2067 3382 3414 4516 4548 9072 9104 2035 Size: 32 1315 32 1102 32 4524 32 2043 Fragments arranged by size: 4524 2043 1315 1102 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2981 3046 4715 6383 1316 Size: 1665 65 1669 1668 4045 Fragments arranged by size: 4045 1669 1668 1665 65 XbaI T'CTAG_A Cuts at: 6448 6448 Size: 9112 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4795 5383 5593 5735 4795 Size: 588 210 142 8172 Fragments arranged by size: 8172 588 210 142 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9112 XmaI C'CCGG_G Cuts at: 1870 6228 6437 1870 Size: 4358 209 4545 Fragments arranged by size: 4545 4358 209 XmnI GAAnn'nnTTC Cuts at: 3360 7241 3360 Size: 3881 5231 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6920 276 Size: 53 83 186 936 5386 2468 Fragments arranged by size: 5386 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SfoI SrfI SwaI