Restriction map for
pFN21AA0354
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII Acc65I AccIII AclI AfaI
AflII AflIII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AvrII
BamHI BbeI BbrPI BbsI BcgI
BciVI BcnI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsaI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII BstEII BstPI BstXI Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoT22I FseI HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SalI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SspBI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 252 GTATAC GT^[AC][GT]AC
AccII 394 CGCG CG^CG
AcsI 1230 GAATTT [GA]^AATT[CT]
AcyI 563 GACGCC G[GA]^CG[CT]C
Aor51HI 422 AGCGCT AGC^GCT
ApaI 1369 GGGCCC GGGCC^C
ApoI 1230 GAATTT [GA]^AATT[CT]
AvaI 683 CTCGGG C^[CT]CG[GA]G
AviII 524 TGCGCA TGC^GCA
BanI 813 GGTGCC G^G[CT][GA]CC
BbvCI 1107 CCTCAGC CCTCAGC(-5/-2)
BglI 482 GCCAGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 464 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 464 GCTCAGC GC^T[ACGT]AGC
BsaHI 563 GACGCC G[GA]^CG[CT]C
BsaWI 654 ACCGGA [AT]^CCGG[AT]
BsmBI 561 GAGACG CGTCTC(1/5)
BsmFI 447 GGGAC GGGAC(10/14)
BsoBI 683 CTCGGG C^[CT]CG[GA]G
BsrDI 281 GCAATG GCAATG(2/0)
BssSI 869 CTCGTG CACGAG(-5/-1)
Bst1107I 252 GTATAC GTA^TAC
BstAPI 1844 GCAAAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1227 TTCGAA TT^CGAA
BstUI 394 CGCG CG^CG
BstZ17I 252 GTATAC GTA^TAC
CelII 464 GCTCAGC GC^T[ACGT]AGC
DraI 103 TTTAAA TTT^AAA
DsaI 860 CCATGG C^C[GA][CT]GG
EarI 516 CTCTTC CTCTTC(1/4)
Eco47III 422 AGCGCT AGC^GCT
EcoNI 1547 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1171 GATATC GAT^ATC
FspI 524 TGCGCA TGC^GCA
HapII 655 CCGG C^CGG
Hin1I 563 GACGCC G[GA]^CG[CT]C
HincII 1749 GTTGAC GT[CT]^[GA]AC
HindII 1749 GTTGAC GT[CT]^[GA]AC
HpaII 655 CCGG C^CGG
Ksp632I 516 CTCTTC CTCTTC(1/4)
MaeII 1702 ACGT A^CGT
MspI 655 CCGG C^CGG
MvnI 394 CGCG CG^CG
NcoI 860 CCATGG C^CATGG
NspV 1227 TTCGAA TT^CGAA
SapI 515 GCTCTTC GCTCTTC(1/4)
SbfI 1360 CCTGCAGG CCTGCA^GG
SfuI 1227 TTCGAA TT^CGAA
SmlI 797 CTCAAG C^T[CT][GA]AG
Sse8387I 1360 CCTGCAGG CCTGCA^GG
SspI 1288 AATATT AAT^ATT
TaqI 1228 TCGA T^CGA
TfiI 706 GATTC G^A[AT]TC
TthHB8I 1228 TCGA T^CGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AocI 943 CCTGAGG CC^T[ACGT]AGG
1015 CCTCAGG
BalI 60 TGGCCA TGG^CCA
1719 TGGCCA
BclI 780 TGATCA T^GATCA
897 TGATCA
BpmI 1551 CTCCAG CTGGAG(16/14)
1318 CTGGAG
BsrBI 114 CCGCTC CCGCTC(-3/-3)
595 GAGCGG
BstYI 1140 AGATCC [GA]^GATC[CT]
1655 AGATCC
Bsu36I 943 CCTGAGG CC^T[ACGT]AGG
1015 CCTCAGG
DraII 1328 AGGCCCC [GA]G^G[ACGT]CC[CT]
1369 GGGCCCT
EaeI 60 TGGCCA [CT]^GGCC[GA]
1719 TGGCCA
Eco81I 943 CCTGAGG CC^T[ACGT]AGG
1015 CCTCAGG
EcoO109I 1328 AGGCCCC [GA]G^G[ACGT]CC[CT]
1369 GGGCCCT
FauI 413 CCCGC CCCGC(4/6)
597 GCGGG
FbaI 780 TGATCA T^GATCA
897 TGATCA
HgaI 366 GACGC GACGC(5/10)
563 GACGC
MflI 1140 AGATCC [GA]^GATC[CT]
1655 AGATCC
MluNI 60 TGGCCA TGG^CCA
1719 TGGCCA
MscI 60 TGGCCA TGG^CCA
1719 TGGCCA
PflMI 1176 CCATATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1348 CCAGAGGCTGG
SpeI 889 ACTAGT A^CTAGT
1303 ACTAGT
SphI 130 GCATGC GCATG^C
416 GCATGC
Van91I 1176 CCATATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1348 CCAGAGGCTGG
XhoII 1140 AGATCC [GA]^GATC[CT]
1655 AGATCC
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
BanII BbvI BfaI BmyI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HhaI HinP1I
HinfI HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI