(Circular) (Six-base) MAPSORT of: pFN21AA0354.seq Check: 6500 from: 1 to: 6736 pFN21AA0354 6736 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2328 3359 2328 Size: 1031 5705 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4546 278 Size: 53 83 186 936 3010 2468 Fragments arranged by size: 3010 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2258 4079 1937 Size: 321 1821 4594 Fragments arranged by size: 4594 1821 321 Acc65I G'GTAC_C Cuts at: 4057 4057 Size: 6736 AclI AA'CG_TT Cuts at: 1563 4863 5236 1563 Size: 3300 373 3063 Fragments arranged by size: 3300 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2658 3468 3956 4799 6183 6451 1632 Size: 1026 810 488 843 1384 268 1917 Fragments arranged by size: 1917 1384 1026 843 810 488 268 AfeI AGC'GCT Cuts at: 2429 2429 Size: 6736 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6539 AflIII A'CryG_T Cuts at: 1203 5635 6612 1203 Size: 4432 977 1327 Fragments arranged by size: 4432 1327 977 AgeI A'CCGG_T Cuts at: 5567 5567 Size: 6736 AhdI GACnn_n'nnGTC Cuts at: 5465 5465 Size: 6736 AleI CACnn'nnGTG Cuts at: 1166 3900 3912 1166 Size: 2734 12 3990 Fragments arranged by size: 3990 2734 12 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6736 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2019 2051 2019 Size: 32 6704 AlwNI CAG_nnn'CTG Cuts at: 2044 2449 3359 4479 6051 2044 Size: 405 910 1120 1572 2729 Fragments arranged by size: 2729 1572 1120 910 405 ApaI G_GGCC'C Cuts at: 3378 3378 Size: 6736 ApaLI G'TGCA_C Cuts at: 4793 5949 4793 Size: 1156 5580 ApoI r'AATT_y Cuts at: 1493 3235 4045 4271 4423 6532 1493 Size: 1742 810 226 152 2109 1697 Fragments arranged by size: 2109 1742 1697 810 226 152 AseI AT'TA_AT Cuts at: 160 1032 5290 160 Size: 872 4258 1606 Fragments arranged by size: 4258 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6736 AvaI C'yCGr_G Cuts at: 1870 1943 2688 4061 1870 Size: 73 745 1373 4545 Fragments arranged by size: 4545 1373 745 73 BamHI G'GATC_C Cuts at: 4066 4433 4066 Size: 367 6369 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2818 4057 5512 618 Size: 325 217 627 1031 1239 1455 1842 Fragments arranged by size: 1842 1455 1239 1031 627 325 217 BanII G_rGCy'C Cuts at: 1391 2158 2471 2522 3011 3378 3656 3835 4055 Size: 767 313 51 489 367 278 179 220 Cuts at: 4055 1391 Size: 4072 Fragments arranged by size: 4072 767 489 367 313 278 220 179 51 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5765 BbvCI CC'TCA_GC Cuts at: 3113 3113 Size: 6736 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4015 4049 4927 4961 1903 Size: 34 2078 34 878 34 3678 Fragments arranged by size: 3678 2078 878 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4628 5838 4628 Size: 1210 5526 BclI T'GATC_A Cuts at: 1548 2785 2902 1548 Size: 1237 117 5382 Fragments arranged by size: 5382 1237 117 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2493 5347 136 Size: 107 122 71 631 605 821 2854 1525 Fragments arranged by size: 2854 1525 821 631 605 122 107 71 BglII A'GATC_T Cuts at: 6731 6731 Size: 6736 BlpI GC'TnA_GC Cuts at: 2470 4153 2470 Size: 1683 5053 Bme1580I G_kGCm'C Cuts at: 1790 2823 3378 4797 5953 1790 Size: 1033 555 1419 1156 2573 Fragments arranged by size: 2573 1419 1156 1033 555 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6736 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2385 3145 4467 Size: 416 53 83 399 24 968 760 1322 Cuts at: 4467 5425 442 Size: 958 1753 Fragments arranged by size: 1753 1322 968 958 760 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6736 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3344 3541 5396 1239 Size: 2105 197 1855 2579 Fragments arranged by size: 2579 2105 1855 197 Bpu10I CC'TnA_GC Cuts at: 3015 3113 3015 Size: 98 6638 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2787 4861 5726 6024 2787 Size: 2074 865 298 3499 Fragments arranged by size: 3499 2074 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5399 882 Size: 617 3900 2219 Fragments arranged by size: 3900 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4527 493 Size: 711 3323 2702 Fragments arranged by size: 3323 2702 711 BsaBI GATnn'nnATC Cuts at: 6730 6730 Size: 6736 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2569 4543 4925 5560 Size: 53 83 186 936 1036 1974 382 635 Cuts at: 5560 275 Size: 1451 Fragments arranged by size: 1974 1451 1036 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2659 5169 5567 5841 5988 6399 2659 Size: 2510 398 274 147 411 2996 Fragments arranged by size: 2996 2510 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3075 3105 1178 Size: 30 165 30 1672 30 4809 Fragments arranged by size: 4809 1672 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2513 2612 2708 3370 3513 3538 3600 1186 Size: 1327 99 96 662 143 25 62 4322 Fragments arranged by size: 4322 1327 662 143 99 96 62 25 BseYI C'CCAG_C Cuts at: 2275 2449 2822 3359 3735 4022 5939 6435 2275 Size: 174 373 537 376 287 1917 496 2576 Fragments arranged by size: 2576 1917 537 496 376 373 287 174 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2223 2898 3497 1163 Size: 573 487 675 599 4402 Fragments arranged by size: 4402 675 599 573 487 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4186 4947 5975 1540 Size: 275 186 2185 761 1028 2301 Fragments arranged by size: 2301 2185 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2471 2522 2976 3011 3656 3810 3835 4797 4882 Size: 51 454 35 645 154 25 962 85 Cuts at: 4882 5953 2471 Size: 1071 3254 Fragments arranged by size: 3254 1071 962 645 454 154 85 51 35 25 BsmI GAATG_Cn' Cuts at: 4247 4340 4247 Size: 93 6643 BsmBI CGTCTCn'nnnn_ Cuts at: 2560 2560 Size: 6736 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2158 2471 2522 2823 2976 3011 3378 Size: 399 368 313 51 301 153 35 367 Cuts at: 3378 3656 3810 3835 4055 4797 4882 5953 1391 Size: 278 154 25 220 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 368 367 313 301 278 220 154 153 85 51 35 25 BspEI T'CCGG_A Cuts at: 6399 6399 Size: 6736 BspHI T'CATG_A Cuts at: 4625 6355 4625 Size: 1730 5006 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2328 3359 3430 4001 4091 844 Size: 1484 1031 71 571 90 3489 Fragments arranged by size: 3489 1484 1031 571 90 71 BsrBI CCG'CTC Cuts at: 1394 1823 2121 2602 4623 1394 Size: 429 298 481 2021 3507 Fragments arranged by size: 3507 2021 481 429 298 BsrDI GCAATG_nn' Cuts at: 65 1248 2293 5231 5405 65 Size: 1183 1045 2938 174 1396 Fragments arranged by size: 2938 1396 1183 1045 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5380 5567 1700 Size: 110 3570 187 2869 Fragments arranged by size: 3570 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6736 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6736 BssSI C'ACGA_G Cuts at: 2874 4796 5808 2874 Size: 1922 1012 3802 Fragments arranged by size: 3802 1922 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3855 794 Size: 3061 3675 BstBI TT'CG_AA Cuts at: 3233 5540 3233 Size: 2307 4429 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6736 BstYI r'GATC_y Cuts at: 1972 3145 3660 4066 4433 4818 4835 6287 6373 Size: 1173 515 406 367 385 17 1452 86 Cuts at: 6373 6385 6731 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1173 515 406 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2259 2259 Size: 6736 Bsu36I CC'TnA_GG Cuts at: 2949 3021 2949 Size: 72 6664 BtgI C'CryG_G Cuts at: 513 1065 2004 2865 513 Size: 552 939 861 4384 Fragments arranged by size: 4384 939 861 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3051 487 Size: 683 56 1825 4172 Fragments arranged by size: 4172 1825 683 56 BtsI GCAGTG_nn' Cuts at: 3133 4252 5046 5066 3133 Size: 1119 794 20 4803 Fragments arranged by size: 4803 1119 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6736 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6701 DraI TTT'AAA Cuts at: 2110 4040 2110 Size: 1930 4806 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5743 809 Size: 507 4427 1802 Fragments arranged by size: 4427 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2065 3724 4183 Size: 54 1425 50 275 18 235 1659 459 Cuts at: 4183 5072 6701 8 Size: 889 1629 43 Fragments arranged by size: 1659 1629 1425 889 459 275 235 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4183 1537 Size: 275 2371 4090 Fragments arranged by size: 4090 2371 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2527 4666 1353 Size: 1174 2139 3423 Fragments arranged by size: 3423 2139 1174 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5323 5697 5843 387 Size: 1064 138 3734 374 146 1280 Fragments arranged by size: 3734 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2469 2520 3009 3654 3833 2469 Size: 51 489 645 179 5372 Fragments arranged by size: 5372 645 489 179 51 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2658 3344 3468 3541 3956 4799 5396 Size: 393 1026 686 124 73 415 843 597 Cuts at: 5396 6183 6451 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1026 843 787 686 597 415 393 268 124 73 EcoNI CCTnn'n_nnAGG Cuts at: 3556 3556 Size: 6736 EcoO109I rG'GnC_Cy Cuts at: 3334 3375 3334 Size: 41 6695 EcoRI G'AATT_C Cuts at: 4045 4423 4045 Size: 378 6358 EcoRV GAT'ATC Cuts at: 3178 3178 Size: 6736 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3814 3814 Size: 6736 FspI TGC'GCA Cuts at: 2531 5242 2531 Size: 2711 4025 HaeII r_GCGC'y Cuts at: 1678 2418 2431 2617 5883 1678 Size: 740 13 186 3266 2531 Fragments arranged by size: 3266 2531 740 186 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2445 2477 3029 3061 3075 Size: 32 135 32 873 32 552 32 14 Cuts at: 3075 3107 3137 3169 3200 3232 3764 3796 3940 Size: 32 30 32 31 32 532 32 144 Cuts at: 3940 3972 4000 4032 5377 5409 5451 5483 1373 Size: 32 28 32 1345 32 42 32 2626 Fragments arranged by size: 2626 1345 873 552 532 144 135 42 32 32 32 32 32 32 32 32 32 32 32 32 31 30 28 14 HincII GTy'rAC Cuts at: 669 1657 1938 3756 4080 4326 669 Size: 988 281 1818 324 246 3079 Fragments arranged by size: 3079 1818 988 324 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6736 HpaI GTT'AAC Cuts at: 4326 4326 Size: 6736 KpnI G_GTAC'C Cuts at: 4061 4061 Size: 6736 MfeI C'AATT_G Cuts at: 4335 4335 Size: 6736 MluI A'CGCG_T Cuts at: 6612 6612 Size: 6736 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2644 5850 6034 1305 Size: 1339 3206 184 2007 Fragments arranged by size: 3206 2007 1339 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2067 3726 10 Size: 54 1425 343 235 1659 3020 Fragments arranged by size: 3020 1659 1425 343 235 54 MslI CAynn'nnrTG Cuts at: 518 1166 2090 2192 2270 2981 3900 3912 4694 Size: 648 924 102 78 711 919 12 782 Cuts at: 4694 5053 5212 518 Size: 359 159 2042 Fragments arranged by size: 2042 924 919 782 711 648 359 159 102 78 12 MspA1I CmG'CkG Cuts at: 1606 2044 2203 2449 4152 4829 5977 6222 1606 Size: 438 159 246 1703 677 1148 245 2120 Fragments arranged by size: 2120 1703 1148 677 438 246 245 159 NcoI C'CATG_G Cuts at: 513 1065 2004 2865 513 Size: 552 939 861 4384 Fragments arranged by size: 4384 939 861 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6736 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6736 NotI GC'GGCC_GC Cuts at: 4183 4183 Size: 6736 NspI r_CATG'y Cuts at: 1132 1229 2139 2349 2425 4094 5639 1132 Size: 97 910 210 76 1669 1545 2229 Fragments arranged by size: 2229 1669 1545 910 210 97 76 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6736 PciI A'CATG_T Cuts at: 5635 5635 Size: 6736 PflMI CCAn_nnn'nTGG Cuts at: 1108 3187 3359 1108 Size: 2079 172 4485 Fragments arranged by size: 4485 2079 172 PmeI GTTT'AAAC Cuts at: 4040 4040 Size: 6736 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4805 4837 6318 6350 4805 Size: 32 1481 32 5191 Fragments arranged by size: 5191 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6736 PsiI TTA'TAA Cuts at: 4306 4306 Size: 6736 PspOMI G'GGCC_C Cuts at: 3374 3374 Size: 6736 PstI C_TGCA'G Cuts at: 830 2596 3320 3370 3427 3772 4088 830 Size: 1766 724 50 57 345 316 3478 Fragments arranged by size: 3478 1766 724 345 316 57 50 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6736 PvuII CAG'CTG Cuts at: 2044 2203 2449 2044 Size: 159 246 6331 Fragments arranged by size: 6331 246 159 SacI G_AGCT'C Cuts at: 2471 2522 3011 3656 3835 2471 Size: 51 489 645 179 5372 Fragments arranged by size: 5372 645 489 179 51 SalI G'TCGA_C Cuts at: 1936 4078 1936 Size: 2142 4594 SapI GCTCTTCn'nnn_ Cuts at: 2527 2527 Size: 6736 SbfI CC_TGCA'GG Cuts at: 3370 4088 3370 Size: 718 6018 ScaI AGT'ACT Cuts at: 4984 6696 4984 Size: 1712 5024 SfcI C'TryA_G Cuts at: 826 1045 2441 2592 3316 3366 3423 3768 4084 Size: 219 1396 151 724 50 57 345 316 Cuts at: 4084 5219 5900 6091 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1396 1135 724 681 345 316 219 191 151 57 50 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6736 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6736 SmaI CCC'GGG Cuts at: 1872 4063 1872 Size: 2191 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2802 4840 5741 6003 820 Size: 197 926 859 2038 901 262 1553 Fragments arranged by size: 2038 1553 926 901 859 262 197 SnaBI TAC'GTA Cuts at: 493 4527 493 Size: 4034 2702 SpeI A'CTAG_T Cuts at: 152 2894 3308 152 Size: 2742 414 3580 Fragments arranged by size: 3580 2742 414 SphI G_CATG'C Cuts at: 1132 2139 2425 4094 1132 Size: 1007 286 1669 3774 Fragments arranged by size: 3774 1669 1007 286 SspI AAT'ATT Cuts at: 5 52 3295 4660 5 Size: 47 3243 1365 2081 Fragments arranged by size: 3243 2081 1365 47 StuI AGG'CCT Cuts at: 1517 2231 3445 3797 1517 Size: 714 1214 352 4456 Fragments arranged by size: 4456 1214 714 352 StyI C'CwwG_G Cuts at: 513 1065 2004 2865 2959 3493 3578 4175 513 Size: 552 939 861 94 534 85 597 3074 Fragments arranged by size: 3074 939 861 597 552 534 94 85 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4763 4780 4933 5118 5563 325 Size: 1255 3183 17 153 185 445 1498 Fragments arranged by size: 3183 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4982 6694 96 Size: 274 80 33 51 1443 3005 1712 138 Fragments arranged by size: 3005 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6696 6728 6696 Size: 32 6704 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6736 XbaI T'CTAG_A Cuts at: 4072 4072 Size: 6736 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2060 2272 3532 2060 Size: 212 1260 5264 Fragments arranged by size: 5264 1260 212 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6736 XmaI C'CCGG_G Cuts at: 1870 4061 1870 Size: 2191 4545 XmnI GAAnn'nnTTC Cuts at: 4865 4865 Size: 6736 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4544 276 Size: 53 83 186 936 3010 2468 Fragments arranged by size: 3010 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI