Restriction map for
pFN21AA0329
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# NO CUT ENZYMES ( 67 enzymes) #
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Acc65I AccI AflII AgeI Aor51HI
AscI AseI AsnI Asp718I AvrII
BciVI BclI BfrI BglII BlnI
BsiEI BsiWI BsmI Bsp1407I BspHI
BspLU11I BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I DraI
EagI EclXI Eco47III Eco52I EcoO65I
EcoT22I FbaI FseI HpaI KpnI
KspI MluI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI PvuI RcaI
SacII SalI SbfI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 2247 GACGTC GACGT^C
AccIII 2898 TCCGGA T^CCGGA
AclI 1635 AACGTT AA^CGTT
AflIII 2145 ACGTGT A^C[GA][CT]GT
AhdI 1507 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1507 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3194 TGCGCA TGC^GCA
BamHI 973 GGATCC G^GATCC
BbeI 2515 GGCGCC GGCGC^C
BsaBI 1070 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2898 TCCGGA T^CCGGA
BspDI 6 ATCGAT AT^CGAT
BspEI 2898 TCCGGA T^CCGGA
BsrBI 93 CCGCTC CCGCTC(-3/-3)
BsrDI 326 CATTGC GCAATG(2/0)
BssHII 3513 GCGCGC G^CGCGC
ClaI 6 ATCGAT AT^CGAT
CpoI 3877 CGGACCG CG^G[AT]CCG
DraIII 3142 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2241 GACATTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1507 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3286 GAATTC G^AATTC
EcoRV 9 GATATC GAT^ATC
FspI 3194 TGCGCA TGC^GCA
HincII 943 GTCAAC GT[CT]^[GA]AC
HindII 943 GTCAAC GT[CT]^[GA]AC
KasI 2515 GGCGCC G^GCGCC
MamI 1070 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 956 CAATTG C^AATTG
MroI 2898 TCCGGA T^CCGGA
MunI 956 CAATTG C^AATTG
NarI 2515 GGCGCC GG^CGCC
PflMI 2553 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1635 AACGTT AA^CGTT
RsrII 3877 CGGACCG CG^G[AT]CCG
SacI 2317 GAGCTC GAGCT^C
ScaI 637 AGTACT AGT^ACT
SexAI 269 ACCTGGT A^CC[AT]GGT
SfoI 2515 GGCGCC GGC^GCC
SmaI 712 CCCGGG CCC^GGG
SspI 1014 AATATT AAT^ATT
Van91I 2553 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 471 TCTAGA T^CTAGA
XmaCI 712 CCCGGG C^CCGGG
XmaI 712 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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Alw44I 999 GTGCAC G^TGCAC
3868 GTGCAC
AocI 2573 CCTCAGG CC^T[ACGT]AGG
4201 CCTGAGG
ApaLI 999 GTGCAC G^TGCAC
3868 GTGCAC
BbrPI 1126 CACGTG CAC^GTG
3871 CACGTG
BcgI 152 GCAGCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2960 GCATAACGCTCG
BlpI 252 GCTGAGC GC^T[ACGT]AGC
3954 GCTGAGC
Bpu1102I 252 GCTGAGC GC^T[ACGT]AGC
3954 GCTGAGC
BsaAI 1126 CACGTG [CT]AC^GT[GA]
3871 CACGTG
BsmBI 1168 GAGACG CGTCTC(1/5)
2302 GAGACG
BspMI 2556 GCAGGT ACCTGC(4/8)
3857 GCAGGT
BsrFI 182 GCCGGC [GA]^CCGG[CT]
2351 GCCGGC
Bsu36I 2573 CCTCAGG CC^T[ACGT]AGG
4201 CCTGAGG
CelII 252 GCTGAGC GC^T[ACGT]AGC
3954 GCTGAGC
Cfr10I 182 GCCGGC [GA]^CCGG[CT]
2351 GCCGGC
EarI 1185 CTCTTC CTCTTC(1/4)
4173 GAAGAG
Eco81I 2573 CCTCAGG CC^T[ACGT]AGG
4201 CCTGAGG
Ksp632I 1185 CTCTTC CTCTTC(1/4)
4173 GAAGAG
NaeI 182 GCCGGC GCC^GGC
2351 GCCGGC
NcoI 1190 CCATGG C^CATGG
3003 CCATGG
NgoMIV 182 GCCGGC G^CCGGC
2351 GCCGGC
PmaCI 1126 CACGTG CAC^GTG
3871 CACGTG
PmlI 1126 CACGTG CAC^GTG
3871 CACGTG
PshAI 2113 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2175 GACTCCGGTC
SapI 4173 GAAGAGC GCTCTTC(1/4)
1184 GCTCTTC
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# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
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AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XcmI
XhoII XmnI