Restriction map for
pFN21AA0329
(Linear) (Six-base) MAPSORT of: KIAA0329.seq Check: 2917 from: 1 to: 4233
KIAA0329.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2547 3848 4233
Size: 2547 1301 385
Fragments arranged by size:
2547 1301 385
AatII G_ACGT'C
Cuts at: 0 2251 4233
Size: 2251 1982
AclI AA'CG_TT
Cuts at: 0 1636 4233
Size: 1636 2597
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 614 1085 3205 4233
Size: 614 471 2120 1028
Fragments arranged by size:
2120 1028 614 471
AflIII A'CryG_T
Cuts at: 0 2145 4233
Size: 2145 2088
AhdI GACnn_n'nnGTC
Cuts at: 0 1512 4233
Size: 1512 2721
AleI CACnn'nnGTG
Cuts at: 0 241 4233
Size: 241 3992
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 181 2524 4233
Size: 181 2343 1709
Fragments arranged by size:
2343 1709 181
AlwNI CAG_nnn'CTG
Cuts at: 0 19 292 1165 1317 1989 2346 2894 3355
Size: 19 273 873 152 672 357 548 461
Cuts at: 3355 3840 4084 4233
Size: 485 244 149
Fragments arranged by size:
873 672 548 485 461 357 273 244
152 149 19
ApaI G_GGCC'C
Cuts at: 0 1971 2865 2994 3978 4114 4233
Size: 1971 894 129 984 136 119
Fragments arranged by size:
1971 984 894 136 129 119
ApaLI G'TGCA_C
Cuts at: 0 999 3868 4233
Size: 999 2869 365
Fragments arranged by size:
2869 999 365
ApoI r'AATT_y
Cuts at: 0 646 1049 1471 3286 4052 4233
Size: 646 403 422 1815 766 181
Fragments arranged by size:
1815 766 646 422 403 181
AvaI C'yCGr_G
Cuts at: 0 712 2418 2968 3241 3273 4233
Size: 712 1706 550 273 32 960
Fragments arranged by size:
1706 960 712 550 273 32
BamHI G'GATC_C
Cuts at: 0 973 4233
Size: 973 3260
BanI G'GyrC_C
Cuts at: 0 185 860 2471 2515 3316 4165 4233
Size: 185 675 1611 44 801 849 68
Fragments arranged by size:
1611 849 801 675 185 68 44
BanII G_rGCy'C
Cuts at: 0 409 485 719 1265 1280 1971 2030 2321
Size: 409 76 234 546 15 691 59 291
Cuts at: 2321 2583 2865 2994 3978 4114 4233
Size: 262 282 129 984 136 119
Fragments arranged by size:
984 691 546 409 291 282 262 234
136 129 119 76 59 15
BbeI G_GCGC'C
Cuts at: 0 2519 4233
Size: 2519 1714
BbsI GAAGACnn'nnnn_
Cuts at: 0 229 1417 1889 4233
Size: 229 1188 472 2344
Fragments arranged by size:
2344 1188 472 229
BbvCI CC'TCA_GC
Cuts at: 0 1682 1960 2077 2440 4233
Size: 1682 278 117 363 1793
Fragments arranged by size:
1793 1682 363 278 117
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 141 175 2949 2983 4233
Size: 141 34 2774 34 1250
Fragments arranged by size:
2774 1250 141 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1275 3602 4118 4233
Size: 1275 2327 516 115
Fragments arranged by size:
2327 1275 516 115
BlpI GC'TnA_GC
Cuts at: 0 253 3955 4233
Size: 253 3702 278
Fragments arranged by size:
3702 278 253
Bme1580I G_kGCm'C
Cuts at: 0 1003 1971 2865 2994 3872 3978 4114 4233
Size: 1003 968 894 129 878 106 136 119
Fragments arranged by size:
1003 968 894 878 136 129 119 106
BmgBI CAC'GTC
Cuts at: 0 3294 4233
Size: 3294 939
BmrI ACTGGGnnnn_n'
Cuts at: 0 650 1485 3737 4233
Size: 650 835 2252 496
Fragments arranged by size:
2252 835 650 496
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2602 4233
Size: 2602 1631
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 1117 1264 1553 1669 4231 4233
Size: 452 665 147 289 116 2562 2
Fragments arranged by size:
2562 665 452 289 147 116 2
Bpu10I CC'TnA_GC
Cuts at: 0 1292 1682 1960 2077 2347 2440 2695 3299
Size: 1292 390 278 117 270 93 255 604
Cuts at: 3299 4233
Size: 934
Fragments arranged by size:
1292 934 604 390 278 270 255 117
93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1509 2239 2365 4233
Size: 1509 730 126 1868
Fragments arranged by size:
1868 1509 730 126
BsaI GGTCTCn'nnnn_
Cuts at: 0 564 2187 2457 3986 4233
Size: 564 1623 270 1529 247
Fragments arranged by size:
1623 1529 564 270 247
BsaAI yAC'GTr
Cuts at: 0 1128 3873 4233
Size: 1128 2745 360
Fragments arranged by size:
2745 1128 360
BsaBI GATnn'nnATC
Cuts at: 0 1074 4233
Size: 1074 3159
BsaHI Gr'CG_yC
Cuts at: 0 2248 2369 2516 4013 4233
Size: 2248 121 147 1497 220
Fragments arranged by size:
2248 1497 220 147 121
BsaWI w'CCGG_w
Cuts at: 0 976 2178 2898 4233
Size: 976 1202 720 1335
Fragments arranged by size:
1335 1202 976 720
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1240 1270 1422 1452 4204 4233
Size: 1240 30 152 30 2752 29
Fragments arranged by size:
2752 1240 152 30 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 523 2275 3261 3907 4117 4233
Size: 46 477 1752 986 646 210 116
Fragments arranged by size:
1752 986 646 477 210 116 46
BseYI C'CCAG_C
Cuts at: 0 494 896 1296 1964 2029 2336 2355 3212
Size: 494 402 400 668 65 307 19 857
Cuts at: 3212 3632 4233
Size: 420 601
Fragments arranged by size:
857 668 601 494 420 402 400 307
65 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 793 1150 1580 2381 3129 3465 3508
Size: 547 246 357 430 801 748 336 43
Cuts at: 3508 4233
Size: 725
Fragments arranged by size:
801 748 725 547 430 357 336 246
43
BsiHKAI G_wGCw'C
Cuts at: 0 1003 1435 2321 3872 3961 4233
Size: 1003 432 886 1551 89 272
Fragments arranged by size:
1551 1003 886 432 272 89
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1162 2296 4233
Size: 1162 1134 1937
Fragments arranged by size:
1937 1162 1134
Bsp1286I G_dGCh'C
Cuts at: 0 409 485 719 1003 1265 1280 1435 1971
Size: 409 76 234 284 262 15 155 536
Cuts at: 1971 2030 2321 2583 2865 2994 3872 3961 3978
Size: 59 291 262 282 129 878 89 17
Cuts at: 3978 4114 4233
Size: 136 119
Fragments arranged by size:
878 536 409 291 284 282 262 262
234 155 136 129 119 89 76 59
17 15
BspEI T'CCGG_A
Cuts at: 0 2898 4233
Size: 2898 1335
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2547 3848 4233
Size: 2547 1301 385
Fragments arranged by size:
2547 1301 385
BsrBI CCG'CTC
Cuts at: 0 95 4233
Size: 95 4138
BsrDI GCAATG_nn'
Cuts at: 0 325 4233
Size: 325 3908
BsrFI r'CCGG_y
Cuts at: 0 182 2351 4233
Size: 182 2169 1882
Fragments arranged by size:
2169 1882 182
BssHII G'CGCG_C
Cuts at: 0 3513 4233
Size: 3513 720
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1203 1729 1897 4233
Size: 1203 526 168 2336
Fragments arranged by size:
2336 1203 526 168
BstYI r'GATC_y
Cuts at: 0 77 468 973 3266 4233
Size: 77 391 505 2293 967
Fragments arranged by size:
2293 967 505 391 77
Bsu36I CC'TnA_GG
Cuts at: 0 2574 4202 4233
Size: 2574 1628 31
Fragments arranged by size:
2574 1628 31
BtgI C'CryG_G
Cuts at: 0 741 1190 1202 2021 2230 2277 3003 3342
Size: 741 449 12 819 209 47 726 339
Cuts at: 3342 3482 3599 4233
Size: 140 117 634
Fragments arranged by size:
819 741 726 634 449 339 209 140
117 47 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 126 2270 2714 4233
Size: 126 2144 444 1519
Fragments arranged by size:
2144 1519 444 126
BtsI GCAGTG_nn'
Cuts at: 0 1779 2687 2917 4233
Size: 1779 908 230 1316
Fragments arranged by size:
1779 1316 908 230
ClaI AT'CG_AT
Cuts at: 0 7 4233
Size: 7 4226
DraIII CAC_nnn'GTG
Cuts at: 0 3147 4233
Size: 3147 1086
DrdI GACnn_nn'nnGTC
Cuts at: 0 2247 4233
Size: 2247 1986
EaeI y'GGCC_r
Cuts at: 0 1193 1205 2070 3149 3561 4233
Size: 1193 12 865 1079 412 672
Fragments arranged by size:
1193 1079 865 672 412 12
EarI CTCTTCn'nnn_
Cuts at: 0 1191 4168 4233
Size: 1191 2977 65
Fragments arranged by size:
2977 1191 65
EcoICRI GAG'CTC
Cuts at: 0 2319 4233
Size: 2319 1914
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 614 1085 1117 1264 1553 1669 3205
Size: 452 162 471 32 147 289 116 1536
Cuts at: 3205 4231 4233
Size: 1026 2
Fragments arranged by size:
1536 1026 471 452 289 162 147 116
32 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 294 2674 3562 4233
Size: 294 2380 888 671
Fragments arranged by size:
2380 888 671 294
EcoO109I rG'GnC_Cy
Cuts at: 0 1968 2343 2422 2733 2861 2862 2991 3438
Size: 1968 375 79 311 128 1 129 447
Cuts at: 3438 4206 4233
Size: 768 27
Fragments arranged by size:
1968 768 447 375 311 129 128 79
27 1
EcoRI G'AATT_C
Cuts at: 0 3286 4233
Size: 3286 947
EcoRV GAT'ATC
Cuts at: 0 11 4233
Size: 11 4222
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 228 2960 4233
Size: 228 2732 1273
Fragments arranged by size:
2732 1273 228
FspI TGC'GCA
Cuts at: 0 3196 4233
Size: 3196 1037
HaeII r_GCGC'y
Cuts at: 0 2519 2848 3458 3550 3955 4233
Size: 2519 329 610 92 405 278
Fragments arranged by size:
2519 610 405 329 278 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 347 379 499 531 973 1005 1077 1109
Size: 347 32 120 32 442 32 72 32
Cuts at: 1109 1240 1272 1422 1454 1498 1530 2748 2780
Size: 131 32 150 32 44 32 1218 32
Cuts at: 2780 3717 3749 4233
Size: 937 32 484
Fragments arranged by size:
1218 937 484 442 347 150 131 120
72 44 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 945 4233
Size: 945 3288
HindIII A'AGCT_T
Cuts at: 0 2942 3408 3657 4233
Size: 2942 466 249 576
Fragments arranged by size:
2942 576 466 249
KasI G'GCGC_C
Cuts at: 0 2515 4233
Size: 2515 1718
MfeI C'AATT_G
Cuts at: 0 956 4233
Size: 956 3277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 485 889 1914 4155 4233
Size: 94 391 404 1025 2241 78
Fragments arranged by size:
2241 1025 404 391 94 78
MscI TGG'CCA
Cuts at: 0 1195 1207 2072 3151 4233
Size: 1195 12 865 1079 1082
Fragments arranged by size:
1195 1082 1079 865 12
MslI CAynn'nnrTG
Cuts at: 0 145 163 241 1727 3604 4233
Size: 145 18 78 1486 1877 629
Fragments arranged by size:
1877 1486 629 145 78 18
MspA1I CmG'CkG
Cuts at: 0 498 531 1253 1964 2172 2808 3257 3492
Size: 498 33 722 711 208 636 449 235
Cuts at: 3492 3948 3980 4233
Size: 456 32 253
Fragments arranged by size:
722 711 636 498 456 449 253 235
208 33 32
NaeI GCC'GGC
Cuts at: 0 184 2353 4233
Size: 184 2169 1880
Fragments arranged by size:
2169 1880 184
NarI GG'CG_CC
Cuts at: 0 2516 4233
Size: 2516 1717
NcoI C'CATG_G
Cuts at: 0 1190 3003 4233
Size: 1190 1813 1230
Fragments arranged by size:
1813 1230 1190
NgoMIV G'CCGG_C
Cuts at: 0 182 2351 4233
Size: 182 2169 1882
Fragments arranged by size:
2169 1882 182
NspI r_CATG'y
Cuts at: 0 168 2098 3609 3668 3925 4233
Size: 168 1930 1511 59 257 308
Fragments arranged by size:
1930 1511 308 257 168 59
PasI CC'CwG_GG
Cuts at: 0 1273 4233
Size: 1273 2960
PflMI CCAn_nnn'nTGG
Cuts at: 0 2559 4233
Size: 2559 1674
PfoI T'CCnGG_A
Cuts at: 0 1646 4233
Size: 1646 2587
PmlI CAC'GTG
Cuts at: 0 1128 3873 4233
Size: 1128 2745 360
Fragments arranged by size:
2745 1128 360
PpuMI rG'GwC_Cy
Cuts at: 0 2343 2422 2733 3438 4206 4233
Size: 2343 79 311 705 768 27
Fragments arranged by size:
2343 768 705 311 79 27
PshAI GACnn'nnGTC
Cuts at: 0 2117 2179 4233
Size: 2117 62 2054
Fragments arranged by size:
2117 2054 62
PsiI TTA'TAA
Cuts at: 0 1045 4233
Size: 1045 3188
PspOMI G'GGCC_C
Cuts at: 0 1967 2861 2990 3974 4110 4233
Size: 1967 894 129 984 136 123
Fragments arranged by size:
1967 984 894 136 129 123
PstI C_TGCA'G
Cuts at: 0 1812 2503 3390 4233
Size: 1812 691 887 843
Fragments arranged by size:
1812 887 843 691
PvuII CAG'CTG
Cuts at: 0 498 531 1964 2172 3492 3948 4233
Size: 498 33 1433 208 1320 456 285
Fragments arranged by size:
1433 1320 498 456 285 208 33
RsrII CG'GwC_CG
Cuts at: 0 3878 4233
Size: 3878 355
SacI G_AGCT'C
Cuts at: 0 2321 4233
Size: 2321 1912
SanDI GG'GwC_CC
Cuts at: 0 2422 4233
Size: 2422 1811
SapI GCTCTTCn'nnn_
Cuts at: 0 1191 4168 4233
Size: 1191 2977 65
Fragments arranged by size:
2977 1191 65
ScaI AGT'ACT
Cuts at: 0 639 4233
Size: 639 3594
SexAI A'CCwGG_T
Cuts at: 0 269 4233
Size: 269 3964
SfcI C'TryA_G
Cuts at: 0 1808 2499 3386 4233
Size: 1808 691 887 847
Fragments arranged by size:
1808 887 847 691
SfoI GGC'GCC
Cuts at: 0 2517 4233
Size: 2517 1716
SmaI CCC'GGG
Cuts at: 0 714 4233
Size: 714 3519
SmlI C'TyrA_G
Cuts at: 0 1524 2218 2380 4233
Size: 1524 694 162 1853
Fragments arranged by size:
1853 1524 694 162
SphI G_CATG'C
Cuts at: 0 168 2098 3609 4233
Size: 168 1930 1511 624
Fragments arranged by size:
1930 1511 624 168
SrfI GCCC'GGGC
Cuts at: 0 714 4233
Size: 714 3519
SspI AAT'ATT
Cuts at: 0 1016 4233
Size: 1016 3217
StuI AGG'CCT
Cuts at: 0 1062 2065 3940 4233
Size: 1062 1003 1875 293
Fragments arranged by size:
1875 1062 1003 293
StyI C'CwwG_G
Cuts at: 0 628 1190 1216 2189 3003 3048 4233
Size: 628 562 26 973 814 45 1185
Fragments arranged by size:
1185 973 814 628 562 45 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 888 2347 3834 4183 4233
Size: 888 1459 1487 349 50
Fragments arranged by size:
1487 1459 888 349 50
TatI w'GTAC_w
Cuts at: 0 47 176 206 637 4233
Size: 47 129 30 431 3596
Fragments arranged by size:
3596 431 129 47 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 636 668 3526 3558 4204 4233
Size: 636 32 2858 32 646 29
Fragments arranged by size:
2858 646 636 32 32 29
Tth111I GACn'n_nGTC
Cuts at: 0 940 2926 4086 4233
Size: 940 1986 1160 147
Fragments arranged by size:
1986 1160 940 147
XbaI T'CTAG_A
Cuts at: 0 471 4233
Size: 471 3762
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 528 1293 3144 4233
Size: 528 765 1851 1089
Fragments arranged by size:
1851 1089 765 528
XmaI C'CCGG_G
Cuts at: 0 712 4233
Size: 712 3521
XmnI GAAnn'nnTTC
Cuts at: 0 88 424 3286 4233
Size: 88 336 2862 947
Fragments arranged by size:
2862 947 336 88
ZraI GAC'GTC
Cuts at: 0 2249 4233
Size: 2249 1984
Enzymes that do cut:
AarI AatII AclI AcuI AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI MfeI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PstI PvuII RsrII SacI SanDI SapI ScaI
SexAI SfcI SfoI SmaI SmlI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AfeI AflII AgeI AloI AscI AseI
AsiSI AvrII BaeI BciVI BclI BfrBI BglII BmtI
BsiEI BsiWI BsmI BspHI BsrGI BssSI BstAPI BstBI
BstEII BstZ17I CspCI DraI EagI EciI FseI FspAI
HpaI KpnI MluI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PpiI PspXI PsrI PvuI SacII
SalI SbfI SfiI SgfI SgrAI SnaBI SpeI SwaI
XhoI