(Circular) (Six-base) MAPSORT of: pFN21AA0310.seq Check: 8507 from: 1 to: 11776 pFN21AA0310 11776 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4918 6760 7639 7650 4918 Size: 1842 879 11 9044 Fragments arranged by size: 9044 1842 879 11 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7158 8592 9586 278 Size: 53 83 186 936 5622 1434 994 2468 Fragments arranged by size: 5622 2468 1434 994 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2179 5322 5577 7038 9119 1937 Size: 242 3143 255 1461 2081 4594 Fragments arranged by size: 4594 3143 2081 1461 255 242 Acc65I G'GTAC_C Cuts at: 9097 9097 Size: 11776 AclI AA'CG_TT Cuts at: 1563 9075 9903 10276 1563 Size: 7512 828 373 3063 Fragments arranged by size: 7512 3063 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2379 2475 2724 3093 3663 4113 4397 5742 Size: 747 96 249 369 570 450 284 1345 Cuts at: 5742 6393 9839 11223 11491 1632 Size: 651 3446 1384 268 1917 Fragments arranged by size: 3446 1917 1384 1345 747 651 570 450 369 284 268 249 96 AflII C'TTAA_G Cuts at: 820 1017 4996 820 Size: 197 3979 7600 Fragments arranged by size: 7600 3979 197 AflIII A'CryG_T Cuts at: 1203 3253 5025 6465 8544 10675 11652 1203 Size: 2050 1772 1440 2079 2131 977 1327 Fragments arranged by size: 2131 2079 2050 1772 1440 1327 977 AgeI A'CCGG_T Cuts at: 3841 10607 3841 Size: 6766 5010 AhdI GACnn_n'nnGTC Cuts at: 3565 4610 6598 7159 10505 3565 Size: 1045 1988 561 3346 4836 Fragments arranged by size: 4836 3346 1988 1045 561 AleI CACnn'nnGTG Cuts at: 1166 3344 4892 4925 5835 7479 7627 1166 Size: 2178 1548 33 910 1644 148 5315 Fragments arranged by size: 5315 2178 1644 1548 910 148 33 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3362 4943 5088 9028 1184 Size: 2178 1581 145 3940 3932 Fragments arranged by size: 3940 3932 2178 1581 145 AlwNI CAG_nnn'CTG Cuts at: 2453 2467 3273 3628 5185 5338 5438 5470 7378 Size: 14 806 355 1557 153 100 32 1908 Cuts at: 7378 7976 8780 9519 11091 2453 Size: 598 804 739 1572 3138 Fragments arranged by size: 3138 1908 1572 1557 806 804 739 598 355 153 100 32 14 ApaI G_GGCC'C Cuts at: 2783 3477 4149 7941 8963 2783 Size: 694 672 3792 1022 5596 Fragments arranged by size: 5596 3792 1022 694 672 ApaLI G'TGCA_C Cuts at: 3551 4014 4061 9833 10989 3551 Size: 463 47 5772 1156 4338 Fragments arranged by size: 5772 4338 1156 463 47 ApoI r'AATT_y Cuts at: 1493 3392 6335 6619 8137 8150 8428 9085 9311 Size: 1899 2943 284 1518 13 278 657 226 Cuts at: 9311 9463 11572 1493 Size: 152 2109 1697 Fragments arranged by size: 2943 2109 1899 1697 1518 657 284 278 226 152 13 AseI AT'TA_AT Cuts at: 160 1032 6540 10330 160 Size: 872 5508 3790 1606 Fragments arranged by size: 5508 3790 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11776 AvaI C'yCGr_G Cuts at: 1870 1943 2677 3018 3451 3665 3784 4115 5651 Size: 73 734 341 433 214 119 331 1536 Cuts at: 5651 5657 5673 5936 6496 6845 6981 7831 7837 Size: 6 16 263 560 349 136 850 6 Cuts at: 7837 7933 8170 8221 9101 1870 Size: 96 237 51 880 4545 Fragments arranged by size: 4545 1536 880 850 734 560 433 349 341 331 263 237 214 136 119 96 73 51 16 6 6 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5719 5752 5719 Size: 33 11743 BamHI G'GATC_C Cuts at: 2161 2500 7295 9106 9473 2161 Size: 339 4795 1811 367 4464 Fragments arranged by size: 4795 4464 1811 367 339 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2725 3346 4084 4919 4927 Size: 325 217 627 938 621 738 835 8 Cuts at: 4927 5104 5188 6221 7382 7807 8098 8179 9097 Size: 177 84 1033 1161 425 291 81 918 Cuts at: 9097 10552 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1161 1033 938 918 835 738 627 621 425 325 291 217 177 84 81 8 BanII G_rGCy'C Cuts at: 1391 2331 2550 2783 2955 3118 3429 3477 3529 Size: 940 219 233 172 163 311 48 52 Cuts at: 3529 3791 4149 4166 4627 4984 5176 5658 7702 Size: 262 358 17 461 357 192 482 2044 Cuts at: 7702 7780 7823 7838 7941 7998 8072 8536 8578 Size: 78 43 15 103 57 74 464 42 Cuts at: 8578 8793 8963 9032 9095 1391 Size: 215 170 69 63 4072 Fragments arranged by size: 4072 2044 940 482 464 461 358 357 311 262 233 219 215 192 172 170 163 103 78 74 69 63 57 52 48 43 42 17 15 BbeI G_GCGC'C Cuts at: 5108 8102 5108 Size: 2994 8782 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4974 6602 928 Size: 971 3075 1628 6102 Fragments arranged by size: 6102 3075 1628 971 BbvCI CC'TCA_GC Cuts at: 2619 4263 6113 7858 2619 Size: 1644 1850 1745 6537 Fragments arranged by size: 6537 1850 1745 1644 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5753 5787 8161 8195 8930 Size: 34 50 34 3732 34 2374 34 735 Cuts at: 8930 8964 9967 10001 1903 Size: 34 1003 34 3678 Fragments arranged by size: 3732 3678 2374 1003 735 50 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4090 4116 5720 9668 10878 4090 Size: 26 1604 3948 1210 4988 Fragments arranged by size: 4988 3948 1604 1210 26 BclI T'GATC_A Cuts at: 1548 4504 7536 1548 Size: 2956 3032 5788 Fragments arranged by size: 5788 3032 2956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2680 4353 5160 Size: 107 122 71 631 605 1008 1673 807 Cuts at: 5160 5347 5662 7936 8002 10387 136 Size: 187 315 2274 66 2385 1525 Fragments arranged by size: 2385 2274 1673 1525 1008 807 631 605 315 187 122 107 71 66 BglII A'GATC_T Cuts at: 11771 11771 Size: 11776 BlpI GC'TnA_GC Cuts at: 3610 4497 4524 8126 8997 9193 3610 Size: 887 27 3602 871 196 6193 Fragments arranged by size: 6193 3602 887 871 196 27 Bme1580I G_kGCm'C Cuts at: 1790 2730 2783 3085 3477 3498 3555 4018 4065 Size: 940 53 302 392 21 57 463 47 Cuts at: 4065 4087 4149 7941 8963 9837 10993 1790 Size: 22 62 3792 1022 874 1156 2573 Fragments arranged by size: 3792 2573 1156 1022 940 874 463 392 302 62 57 53 47 22 21 BmgBI CAC'GTC Cuts at: 1318 6468 1318 Size: 5150 6626 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2213 2436 2826 Size: 416 53 83 399 24 796 223 390 Cuts at: 2826 2947 3065 5827 7366 7558 7913 9507 10465 Size: 121 118 2762 1539 192 355 1594 958 Cuts at: 10465 442 Size: 1753 Fragments arranged by size: 2762 1753 1594 1539 958 796 416 399 390 355 223 192 121 118 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2088 1056 Size: 1032 10744 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3181 3181 Size: 11776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2037 3202 4001 4269 4467 4600 5770 5977 Size: 798 1165 799 268 198 133 1170 207 Cuts at: 5977 6174 7698 7964 8012 8039 8550 8827 10436 Size: 197 1524 266 48 27 511 277 1609 Cuts at: 10436 1239 Size: 2579 Fragments arranged by size: 2579 1609 1524 1170 1165 799 798 511 277 268 266 207 198 197 133 48 27 Bpu10I CC'TnA_GC Cuts at: 2561 2619 2954 4263 4685 6113 7824 7858 2561 Size: 58 335 1309 422 1428 1711 34 6479 Fragments arranged by size: 6479 1711 1428 1309 422 335 58 34 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4397 9016 9901 10766 11064 4397 Size: 4619 885 865 298 5109 Fragments arranged by size: 5109 4619 885 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3728 3908 3998 6006 6684 6848 7382 Size: 617 2229 180 90 2008 678 164 534 Cuts at: 7382 8266 10439 882 Size: 884 2173 2219 Fragments arranged by size: 2229 2219 2173 2008 884 678 617 534 180 164 90 BsaAI yAC'GTr Cuts at: 493 1204 3256 7077 7629 8906 9567 493 Size: 711 2052 3821 552 1277 661 2702 Fragments arranged by size: 3821 2702 2052 1277 711 661 552 BsaBI GATnn'nnATC Cuts at: 11770 11770 Size: 11776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5105 7155 8099 8589 Size: 53 83 186 936 3572 2050 944 490 Cuts at: 8589 9583 9965 10600 275 Size: 994 382 635 1451 Fragments arranged by size: 3572 2050 1451 994 944 936 635 490 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3521 3841 5539 5868 7052 10209 10607 10881 11028 Size: 320 1698 329 1184 3157 398 274 147 Cuts at: 11028 11439 3521 Size: 411 3858 Fragments arranged by size: 3858 3157 1698 1184 411 398 329 320 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2172 2202 3431 3461 4200 Size: 30 165 30 769 30 1229 30 739 Cuts at: 4200 4230 6668 6698 8037 8067 8523 8553 1178 Size: 30 2438 30 1339 30 456 30 4401 Fragments arranged by size: 4401 2438 1339 1229 769 739 456 165 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3170 4415 4418 5343 5677 6494 6794 6797 Size: 1984 1245 3 925 334 817 300 3 Cuts at: 6797 7622 8462 8830 8880 1186 Size: 825 840 368 50 4082 Fragments arranged by size: 4082 1984 1245 925 840 825 817 368 334 300 50 3 3 BseYI C'CCAG_C Cuts at: 2042 2245 2596 2717 2818 3062 3296 3771 4165 Size: 203 351 121 101 244 234 475 394 Cuts at: 4165 4794 5175 5196 5666 6798 7584 8610 8966 Size: 629 381 21 470 1132 786 1026 356 Cuts at: 8966 10979 11475 2042 Size: 2013 496 2343 Fragments arranged by size: 2343 2013 1132 1026 786 629 496 475 470 394 381 356 351 244 234 203 121 101 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2448 2678 3958 4361 4896 5424 6059 Size: 573 712 230 1280 403 535 528 635 Cuts at: 6059 7571 7797 7962 1163 Size: 1512 226 165 4977 Fragments arranged by size: 4977 1512 1280 712 635 573 535 528 403 230 226 165 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2554 9226 9987 11015 1540 Size: 275 186 553 6672 761 1028 2301 Fragments arranged by size: 6672 2301 1028 761 553 275 186 BsiHKAI G_wGCw'C Cuts at: 2362 2955 2985 3118 3341 3555 4018 4065 4855 Size: 593 30 133 223 214 463 47 790 Cuts at: 4855 5916 6060 7830 8578 9032 9837 9922 10993 Size: 1061 144 1770 748 454 805 85 1071 Cuts at: 10993 2362 Size: 3145 Fragments arranged by size: 3145 1770 1071 1061 805 790 748 593 463 454 223 214 144 133 85 47 30 BsiWI C'GTAC_G Cuts at: 3041 3041 Size: 11776 BsmI GAATG_Cn' Cuts at: 9287 9380 9287 Size: 93 11683 BsmBI CGTCTCn'nnnn_ Cuts at: 2095 2517 2838 7080 2095 Size: 422 321 4242 6791 Fragments arranged by size: 6791 4242 422 321 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2331 2362 2550 2730 2783 2955 2985 Size: 399 541 31 188 180 53 172 30 Cuts at: 2985 3085 3118 3341 3429 3477 3498 3529 3555 Size: 100 33 223 88 48 21 31 26 Cuts at: 3555 3791 4018 4065 4087 4149 4166 4627 4855 Size: 236 227 47 22 62 17 461 228 Cuts at: 4855 4984 5176 5658 5916 6060 7702 7780 7823 Size: 129 192 482 258 144 1642 78 43 Cuts at: 7823 7830 7838 7941 7998 8072 8536 8578 8793 Size: 7 8 103 57 74 464 42 215 Cuts at: 8793 8963 9032 9095 9837 9922 10993 1391 Size: 170 69 63 742 85 1071 2174 Fragments arranged by size: 2174 1642 1071 742 541 482 464 461 399 258 236 228 227 223 215 192 188 180 172 170 144 129 103 100 88 85 78 74 69 63 62 57 53 48 47 43 42 33 31 31 30 26 22 21 17 8 7 BspEI T'CCGG_A Cuts at: 7052 11439 7052 Size: 4387 7389 BspHI T'CATG_A Cuts at: 2788 4561 6987 9665 11395 2788 Size: 1773 2426 2678 1730 3169 Fragments arranged by size: 3169 2678 2426 1773 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4615 4918 5122 6760 7400 7639 7650 9131 Size: 3771 303 204 1638 640 239 11 1481 Cuts at: 9131 844 Size: 3489 Fragments arranged by size: 3771 3489 1638 1481 640 303 239 204 11 BsrBI CCG'CTC Cuts at: 1394 1823 5662 7657 8258 9663 1394 Size: 429 3839 1995 601 1405 3507 Fragments arranged by size: 3839 3507 1995 1405 601 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3307 6669 6921 10271 10445 65 Size: 1183 2059 3362 252 3350 174 1396 Fragments arranged by size: 3362 3350 2059 1396 1183 252 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2152 2985 3841 5490 6036 6164 6421 Size: 110 342 833 856 1649 546 128 257 Cuts at: 6421 8623 10420 10607 1700 Size: 2202 1797 187 2869 Fragments arranged by size: 2869 2202 1797 1649 856 833 546 342 257 187 128 110 BsrGI T'GTAC_A Cuts at: 96 5028 96 Size: 4932 6844 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11776 BssSI C'ACGA_G Cuts at: 9836 10848 9836 Size: 1012 10764 BstAPI GCAn_nnn'nTGC Cuts at: 794 3273 3550 4850 9067 794 Size: 2479 277 1300 4217 3503 Fragments arranged by size: 4217 3503 2479 1300 277 BstBI TT'CG_AA Cuts at: 3837 10580 3837 Size: 6743 5033 BstEII G'GTnAC_C Cuts at: 1184 2590 1184 Size: 1406 10370 BstXI CCAn_nnnn'nTGG Cuts at: 2531 2646 5817 7171 7633 2531 Size: 115 3171 1354 462 6674 Fragments arranged by size: 6674 3171 1354 462 115 BstYI r'GATC_y Cuts at: 1972 2161 2500 5806 7295 9106 9473 9858 9875 Size: 189 339 3306 1489 1811 367 385 17 Cuts at: 9875 11327 11413 11425 11771 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 3306 1977 1811 1489 1452 385 367 346 339 189 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5463 8850 5463 Size: 3387 8389 BtgI C'CryG_G Cuts at: 513 1065 3592 3757 4050 5420 6297 6654 7122 Size: 552 2527 165 293 1370 877 357 468 Cuts at: 7122 7179 513 Size: 57 5110 Fragments arranged by size: 5110 2527 1370 877 552 468 357 293 165 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3137 4310 7474 487 Size: 683 56 1911 1173 3164 4789 Fragments arranged by size: 4789 3164 1911 1173 683 56 BtsI GCAGTG_nn' Cuts at: 2130 3539 3941 4711 6250 6768 7718 7925 9292 Size: 1409 402 770 1539 518 950 207 1367 Cuts at: 9292 10086 10106 2130 Size: 794 20 3800 Fragments arranged by size: 3800 1539 1409 1367 950 794 770 518 402 207 20 ClaI AT'CG_AT Cuts at: 1554 3702 8393 1554 Size: 2148 4691 4937 Fragments arranged by size: 4937 4691 2148 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2217 2252 529 Size: 35 1653 35 10053 Fragments arranged by size: 10053 1653 35 35 DraI TTT'AAA Cuts at: 8433 9080 8433 Size: 647 11129 DraIII CAC_nnn'GTG Cuts at: 6157 8021 9067 6157 Size: 1864 1046 8866 Fragments arranged by size: 8866 1864 1046 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2029 2588 10783 809 Size: 507 713 559 8195 1802 Fragments arranged by size: 8195 1802 713 559 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2880 5392 5423 Size: 54 1425 50 275 18 1050 2512 31 Cuts at: 5423 5472 5696 6093 6507 6886 7458 7617 7890 Size: 49 224 397 414 379 572 159 273 Cuts at: 7890 8766 9049 9223 10112 11741 8 Size: 876 283 174 889 1629 43 Fragments arranged by size: 2512 1629 1425 1050 889 876 572 414 397 379 283 275 273 224 174 159 54 50 49 43 31 18 EagI C'GGCC_G Cuts at: 1537 1812 9223 1537 Size: 275 7411 4090 Fragments arranged by size: 7411 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2836 4115 4284 4860 6021 6771 7490 7580 Size: 1483 1279 169 576 1161 750 719 90 Cuts at: 7580 7599 8439 8551 9706 1353 Size: 19 840 112 1155 3423 Fragments arranged by size: 3423 1483 1279 1161 1155 840 750 719 576 169 112 90 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3228 3914 7460 10363 10737 10883 Size: 1064 138 1639 686 3546 2903 374 146 Cuts at: 10883 387 Size: 1280 Fragments arranged by size: 3546 2903 1639 1280 1064 686 374 146 138 EcoICRI GAG'CTC Cuts at: 2953 3116 8576 9030 2953 Size: 163 5460 454 5699 Fragments arranged by size: 5699 5460 454 163 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2037 2379 2475 2724 3093 3202 3663 Size: 393 405 342 96 249 369 109 461 Cuts at: 3663 4001 4113 4269 4397 4467 4600 5742 5770 Size: 338 112 156 128 70 133 1142 28 Cuts at: 5770 5977 6174 6393 7698 7964 8012 8039 8550 Size: 207 197 219 1305 266 48 27 511 Cuts at: 8550 8827 9839 10436 11223 11491 1239 Size: 277 1012 597 787 268 1524 Fragments arranged by size: 1524 1305 1142 1012 787 597 511 461 405 393 369 342 338 277 268 266 249 219 207 197 156 133 128 112 109 96 70 48 28 27 EcoNI CCTnn'n_nnAGG Cuts at: 2774 3228 3275 4999 6411 7646 8842 8952 2774 Size: 454 47 1724 1412 1235 1196 110 5598 Fragments arranged by size: 5598 1724 1412 1235 1196 454 110 47 EcoO109I rG'GnC_Cy Cuts at: 2318 2708 2779 2780 3280 3293 3474 3618 4137 Size: 390 71 1 500 13 181 144 519 Cuts at: 4137 4145 4408 5467 5604 5851 6178 6500 7204 Size: 8 263 1059 137 247 327 322 704 Cuts at: 7204 7937 7938 7952 7973 8194 8225 8522 8607 Size: 733 1 14 21 221 31 297 85 Cuts at: 8607 8744 8761 8959 8960 2318 Size: 137 17 198 1 5134 Fragments arranged by size: 5134 1059 733 704 519 500 390 327 322 297 263 247 221 198 181 144 137 137 85 71 31 21 17 14 13 8 1 1 1 EcoRI G'AATT_C Cuts at: 9085 9463 9085 Size: 378 11398 EcoRV GAT'ATC Cuts at: 5717 5717 Size: 11776 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5022 6339 7303 5022 Size: 1317 964 9495 Fragments arranged by size: 9495 1317 964 FspI TGC'GCA Cuts at: 10282 10282 Size: 11776 HaeII r_GCGC'y Cuts at: 1678 2189 4244 5108 8102 10923 1678 Size: 511 2055 864 2994 2821 2531 Fragments arranged by size: 2994 2821 2531 2055 864 511 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2172 2204 3200 3232 3431 Size: 32 135 32 600 32 996 32 199 Cuts at: 3431 3463 3551 3583 4200 4232 4283 4315 4596 Size: 32 88 32 617 32 51 32 281 Cuts at: 4596 4628 5950 5982 6385 6417 6584 6616 6770 Size: 32 1322 32 403 32 167 32 154 Cuts at: 6770 6802 7145 7177 8037 8069 10417 10449 10491 Size: 32 343 32 860 32 2348 32 42 Cuts at: 10491 10523 1373 Size: 32 2626 Fragments arranged by size: 2626 2348 1322 996 860 617 600 403 343 281 199 167 154 135 88 51 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5323 8587 9120 9366 669 Size: 988 281 3385 3264 533 246 3079 Fragments arranged by size: 3385 3264 3079 988 533 281 246 HindIII A'AGCT_T Cuts at: 748 3675 7280 8364 748 Size: 2927 3605 1084 4160 Fragments arranged by size: 4160 3605 2927 1084 HpaI GTT'AAC Cuts at: 9366 9366 Size: 11776 KasI G'GCGC_C Cuts at: 5104 8098 5104 Size: 2994 8782 KpnI G_GTAC'C Cuts at: 9101 9101 Size: 11776 MfeI C'AATT_G Cuts at: 9375 9375 Size: 11776 MluI A'CGCG_T Cuts at: 11652 11652 Size: 11776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2156 3637 4694 7158 7728 8086 8498 10890 Size: 851 1481 1057 2464 570 358 412 2392 Cuts at: 10890 11074 1305 Size: 184 2007 Fragments arranged by size: 2464 2392 2007 1481 1057 851 570 412 358 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2882 5425 6095 6509 7460 Size: 54 1425 343 1050 2543 670 414 951 Cuts at: 7460 7892 8768 9051 10 Size: 432 876 283 2735 Fragments arranged by size: 2735 2543 1425 1050 951 876 670 432 414 343 283 54 MslI CAynn'nnrTG Cuts at: 518 1166 2295 2529 2583 3344 4892 4925 5835 Size: 648 1129 234 54 761 1548 33 910 Cuts at: 5835 6354 7479 7627 7692 9734 10093 10252 518 Size: 519 1125 148 65 2042 359 159 2042 Fragments arranged by size: 2042 2042 1548 1129 1125 910 761 648 519 359 234 159 148 65 54 33 MspA1I CmG'CkG Cuts at: 1606 2721 3360 3491 3947 5338 5402 7588 8614 Size: 1115 639 131 456 1391 64 2186 1026 Cuts at: 8614 8970 9001 9192 9869 11017 11262 1606 Size: 356 31 191 677 1148 245 2120 Fragments arranged by size: 2186 2120 1391 1148 1115 1026 677 639 456 356 245 191 131 64 31 NaeI GCC'GGC Cuts at: 6423 8625 6423 Size: 2202 9574 NarI GG'CG_CC Cuts at: 5105 8099 5105 Size: 2994 8782 NcoI C'CATG_G Cuts at: 513 1065 6297 7122 7179 513 Size: 552 5232 825 57 5110 Fragments arranged by size: 5232 5110 825 552 57 NdeI CA'TA_TG Cuts at: 387 387 Size: 11776 NgoMIV G'CCGG_C Cuts at: 6421 8623 6421 Size: 2202 9574 NheI G'CTAG_C Cuts at: 1052 2084 1052 Size: 1032 10744 NotI GC'GGCC_GC Cuts at: 9223 9223 Size: 11776 NruI TCG'CGA Cuts at: 7493 7493 Size: 11776 NspI r_CATG'y Cuts at: 1132 1229 2300 2366 2542 4069 8548 9134 10679 Size: 97 1071 66 176 1527 4479 586 1545 Cuts at: 10679 1132 Size: 2229 Fragments arranged by size: 4479 2229 1545 1527 1071 586 176 97 66 PasI CC'CwG_GG Cuts at: 1348 3224 4141 5149 7378 7780 7970 8078 8201 Size: 1876 917 1008 2229 402 190 108 123 Cuts at: 8201 8519 8860 1348 Size: 318 341 4264 Fragments arranged by size: 4264 2229 1876 1008 917 402 341 318 190 123 108 PciI A'CATG_T Cuts at: 8544 10675 8544 Size: 2131 9645 PflMI CCAn_nnn'nTGG Cuts at: 1108 3055 3628 4205 6157 6266 6445 6529 7378 Size: 1947 573 577 1952 109 179 84 849 Cuts at: 7378 1108 Size: 5506 Fragments arranged by size: 5506 1952 1947 849 577 573 179 109 84 PfoI T'CCnGG_A Cuts at: 2491 2965 4577 4858 7163 7989 8055 8527 9023 Size: 474 1612 281 2305 826 66 472 496 Cuts at: 9023 2491 Size: 5244 Fragments arranged by size: 5244 2305 1612 826 496 474 472 281 66 PmeI GTTT'AAAC Cuts at: 9080 9080 Size: 11776 PmlI CAC'GTG Cuts at: 3256 7077 7629 8906 3256 Size: 3821 552 1277 6126 Fragments arranged by size: 6126 3821 1277 552 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4310 4342 8534 8560 8566 8592 9845 9877 11358 Size: 32 4192 26 6 26 1253 32 1481 Cuts at: 11358 11390 4310 Size: 32 4696 Fragments arranged by size: 4696 4192 1481 1253 32 32 32 26 26 6 PpuMI rG'GwC_Cy Cuts at: 2318 2708 4137 5467 5851 6500 7952 8225 8607 Size: 390 1429 1330 384 649 1452 273 382 Cuts at: 8607 2318 Size: 5487 Fragments arranged by size: 5487 1452 1429 1330 649 390 384 382 273 PshAI GACnn'nnGTC Cuts at: 1613 3920 5328 8223 1613 Size: 2307 1408 2895 5166 Fragments arranged by size: 5166 2895 2307 1408 PsiI TTA'TAA Cuts at: 9346 9346 Size: 11776 PspOMI G'GGCC_C Cuts at: 2779 3473 4145 7937 8959 2779 Size: 694 672 3792 1022 5596 Fragments arranged by size: 5596 3792 1022 694 672 PspXI vC'TCGA_Gb Cuts at: 7831 8170 7831 Size: 339 11437 PstI C_TGCA'G Cuts at: 830 2634 4234 4476 4612 6462 7032 7647 8037 Size: 1804 1600 242 136 1850 570 615 390 Cuts at: 8037 8752 8812 8956 9128 830 Size: 715 60 144 172 3478 Fragments arranged by size: 3478 1850 1804 1600 715 615 570 390 242 172 144 136 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11776 PvuII CAG'CTG Cuts at: 3491 3947 5338 5402 7588 9001 3491 Size: 456 1391 64 2186 1413 6266 Fragments arranged by size: 6266 2186 1413 1391 456 64 SacI G_AGCT'C Cuts at: 2955 3118 8578 9032 2955 Size: 163 5460 454 5699 Fragments arranged by size: 5699 5460 454 163 SalI G'TCGA_C Cuts at: 1936 5321 9118 1936 Size: 3385 3797 4594 Fragments arranged by size: 4594 3797 3385 SanDI GG'GwC_CC Cuts at: 2318 5851 6500 8225 8607 2318 Size: 3533 649 1725 382 5487 Fragments arranged by size: 5487 3533 1725 649 382 SapI GCTCTTCn'nnn_ Cuts at: 4860 7490 8551 4860 Size: 2630 1061 8085 Fragments arranged by size: 8085 2630 1061 SbfI CC_TGCA'GG Cuts at: 9128 9128 Size: 11776 ScaI AGT'ACT Cuts at: 5522 10024 11736 5522 Size: 4502 1712 5562 Fragments arranged by size: 5562 4502 1712 SexAI A'CCwGG_T Cuts at: 6992 8017 9063 6992 Size: 1025 1046 9705 Fragments arranged by size: 9705 1046 1025 SfcI C'TryA_G Cuts at: 826 1045 2630 3577 3801 4230 4472 4608 5578 Size: 219 1585 947 224 429 242 136 970 Cuts at: 5578 5731 5833 6458 7028 7643 8033 8748 8808 Size: 153 102 625 570 615 390 715 60 Cuts at: 8808 8881 8952 9124 10259 10940 11131 826 Size: 73 71 172 1135 681 191 1471 Fragments arranged by size: 1585 1471 1135 970 947 715 681 625 615 570 429 390 242 224 219 191 172 153 136 102 73 71 60 SfiI GGCCn_nnn'nGGCC Cuts at: 5160 5160 Size: 11776 SfoI GGC'GCC Cuts at: 5106 8100 5106 Size: 2994 8782 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11776 SmaI CCC'GGG Cuts at: 1872 3020 6498 7935 9103 1872 Size: 1148 3478 1437 1168 4545 Fragments arranged by size: 4545 3478 1437 1168 1148 SmlI C'TyrA_G Cuts at: 820 1017 1943 4376 4996 6845 7831 8170 9031 Size: 197 926 2433 620 1849 986 339 861 Cuts at: 9031 9880 10781 11043 820 Size: 849 901 262 1553 Fragments arranged by size: 2433 1849 1553 986 926 901 861 849 620 339 262 197 SnaBI TAC'GTA Cuts at: 493 9567 493 Size: 9074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 11776 SphI G_CATG'C Cuts at: 1132 9134 1132 Size: 8002 3774 SspI AAT'ATT Cuts at: 5 52 3810 9700 5 Size: 47 3758 5890 2081 Fragments arranged by size: 5890 3758 2081 47 StuI AGG'CCT Cuts at: 1517 4126 6452 8848 9011 1517 Size: 2609 2326 2396 163 4282 Fragments arranged by size: 4282 2609 2396 2326 163 StyI C'CwwG_G Cuts at: 513 1065 3133 4092 6297 6426 7122 7179 7614 Size: 552 2068 959 2205 129 696 57 435 Cuts at: 7614 8793 9215 513 Size: 1179 422 3074 Fragments arranged by size: 3074 2205 2068 1179 959 696 552 435 422 129 57 SwaI ATTT'AAAT Cuts at: 8433 8433 Size: 11776 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2426 2568 3066 6536 6680 6872 7370 Size: 1255 846 142 498 3470 144 192 498 Cuts at: 7370 7639 8413 9803 9820 9973 10158 10603 325 Size: 269 774 1390 17 153 185 445 1498 Fragments arranged by size: 3470 1498 1390 1255 846 774 498 498 445 269 192 185 153 144 142 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2195 2903 5028 Size: 274 80 33 51 1443 218 708 2125 Cuts at: 5028 5520 8547 10022 11734 96 Size: 492 3027 1475 1712 138 Fragments arranged by size: 3027 2125 1712 1475 1443 708 492 274 218 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2728 2760 6805 6837 7508 7540 7971 8003 8523 Size: 32 4045 32 671 32 431 32 520 Cuts at: 8523 8555 11736 11768 2728 Size: 32 3181 32 2736 Fragments arranged by size: 4045 3181 2736 671 520 431 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 6284 7764 8645 1316 Size: 4968 1480 881 4447 Fragments arranged by size: 4968 4447 1480 881 XbaI T'CTAG_A Cuts at: 8552 9112 8552 Size: 560 11216 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2864 3467 3629 3755 4427 5156 5798 7250 7798 Size: 603 162 126 672 729 642 1452 548 Cuts at: 7798 2864 Size: 6842 Fragments arranged by size: 6842 1452 729 672 642 603 548 162 126 XhoI C'TCGA_G Cuts at: 1943 6845 7831 8170 1943 Size: 4902 986 339 5549 Fragments arranged by size: 5549 4902 986 339 XmaI C'CCGG_G Cuts at: 1870 3018 6496 7933 9101 1870 Size: 1148 3478 1437 1168 4545 Fragments arranged by size: 4545 3478 1437 1168 1148 XmnI GAAnn'nnTTC Cuts at: 3396 9905 3396 Size: 6509 5267 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7156 8590 9584 276 Size: 53 83 186 936 5622 1434 994 2468 Fragments arranged by size: 5622 2468 1434 994 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BstZ17I FseI FspAI NsiI PacI PsrI RsrII SacII SrfI