Restriction map for
pFN21AA0284
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# NO CUT ENZYMES ( 84 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I ApaLI AscI
AseI AsnI Asp700I Asp718I BciVI
BclI BfrI BglII BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI EcoO65I EcoRI EcoRV EcoT22I
FbaI HincII HindII HindIII HpaI
KpnI KspI MfeI MluI MroI
MseI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfuI SgrAI
SnaBI Sse8387I SspBI SspI SwaI
Tru9I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 26 enzymes) #
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AcsI 4476 GAATTT [GA]^AATT[CT]
AhdI 2632 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1040 AGCGCT AGC^GCT
ApoI 4476 GAATTT [GA]^AATT[CT]
AspEI 2632 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1555 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2253 CCTAGG C^CTAGG
BamHI 2016 GGATCC G^GATCC
BbrPI 2121 CACGTG CAC^GTG
BlnI 2253 CCTAGG C^CTAGG
BsaAI 2121 CACGTG [CT]AC^GT[GA]
BspLU11I 71 ACATGT A^CATGT
DrdI 811 GACCTGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2632 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1996 CTCTTC CTCTTC(1/4)
Eco47III 1040 AGCGCT AGC^GCT
FseI 3250 GGCCGGCC GGCCGG^CC
Ksp632I 1996 CTCTTC CTCTTC(1/4)
PflFI 1555 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2121 CACGTG CAC^GTG
PmlI 2121 CACGTG CAC^GTG
SexAI 1556 ACCAGGT A^CC[AT]GGT
SpeI 3337 ACTAGT A^CTAGT
SphI 1877 GCATGC GCATG^C
TfiI 64 GATTC G^A[AT]TC
Tth111I 1555 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AflIII 71 ACATGT A^C[GA][CT]GT
3560 ACACGT
AviII 2 TGCGCA TGC^GCA
1874 TGCGCA
BbsI 1200 GAAGAC GAAGAC(2/6)
4245 GAAGAC
BcgI 758 CGAGCCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2358 CGACGGCCCTGC
BpuAI 1200 GAAGAC GAAGAC(2/6)
4245 GAAGAC
BsaBI 630 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4161 GATGAAGATC
BsmBI 2536 GAGACG CGTCTC(1/5)
2542 GAGACG
BstAPI 3352 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3751 GCAGAGATTGC
BstXI 1394 CCAGCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1557 CCAGGTCTTTGG
DraIII 1703 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3823 CACGATGTG
FspI 2 TGCGCA TGC^GCA
1874 TGCGCA
MamI 630 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4161 GATGAAGATC
MslI 72 CATGTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1524 CACGGAGGTG
NcoI 1386 CCATGG C^CATGG
3419 CCATGG
SacI 400 GAGCTC GAGCT^C
1320 GAGCTC
SmlI 118 CTCAAG C^T[CT][GA]AG
1189 CTCAAG
Tsp509I 2581 AATT ^AATT
4477 AATT
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# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI SfoI SmaI StuI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI