(Circular) (Six-base) MAPSORT of: pFN21AA0266.seq Check: 7706 from: 1 to: 7003 pFN21AA0266 7003 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4813 278 Size: 53 83 186 936 3277 2468 Fragments arranged by size: 3277 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4346 1937 Size: 2409 4594 Acc65I G'GTAC_C Cuts at: 4324 4324 Size: 7003 AclI AA'CG_TT Cuts at: 1563 5130 5503 1563 Size: 3567 373 3063 Fragments arranged by size: 3567 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2256 2468 2666 5066 6450 6718 1632 Size: 624 212 198 2400 1384 268 1917 Fragments arranged by size: 2400 1917 1384 624 268 212 198 AflII C'TTAA_G Cuts at: 820 1017 2891 820 Size: 197 1874 4932 Fragments arranged by size: 4932 1874 197 AflIII A'CryG_T Cuts at: 1203 4277 5902 6879 1203 Size: 3074 1625 977 1327 Fragments arranged by size: 3074 1625 1327 977 AgeI A'CCGG_T Cuts at: 5834 5834 Size: 7003 AhdI GACnn_n'nnGTC Cuts at: 5732 5732 Size: 7003 AleI CACnn'nnGTG Cuts at: 1166 3053 1166 Size: 1887 5116 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7003 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3054 3086 3054 Size: 32 6971 AlwNI CAG_nnn'CTG Cuts at: 2532 3135 4746 6318 2532 Size: 603 1611 1572 3217 Fragments arranged by size: 3217 1611 1572 603 ApaLI G'TGCA_C Cuts at: 5060 6216 5060 Size: 1156 5847 ApoI r'AATT_y Cuts at: 1493 2562 3252 3266 3988 4312 4538 4690 6799 Size: 1069 690 14 722 324 226 152 2109 Cuts at: 6799 1493 Size: 1697 Fragments arranged by size: 2109 1697 1069 722 690 324 226 152 14 AseI AT'TA_AT Cuts at: 160 1032 5557 160 Size: 872 4525 1606 Fragments arranged by size: 4525 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7003 AvaI C'yCGr_G Cuts at: 1870 1943 2691 4328 1870 Size: 73 748 1637 4545 Fragments arranged by size: 4545 1637 748 73 BamHI G'GATC_C Cuts at: 4112 4333 4700 4112 Size: 221 367 6415 Fragments arranged by size: 6415 367 221 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4324 5779 618 Size: 325 217 627 2537 1455 1842 Fragments arranged by size: 2537 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2280 2692 2698 3306 4322 1391 Size: 889 412 6 608 1016 4072 Fragments arranged by size: 4072 1016 889 608 412 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4215 928 Size: 971 2316 3716 Fragments arranged by size: 3716 2316 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3073 3107 5194 5228 1903 Size: 34 1136 34 2087 34 3678 Fragments arranged by size: 3678 2087 1136 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4895 6105 4895 Size: 1210 5793 BclI T'GATC_A Cuts at: 1548 3672 1548 Size: 2124 4879 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5614 136 Size: 107 122 71 631 605 3942 1525 Fragments arranged by size: 3942 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2266 3296 6998 2266 Size: 1030 3702 2271 Fragments arranged by size: 3702 2271 1030 BlpI GC'TnA_GC Cuts at: 3318 4420 3318 Size: 1102 5901 Bme1580I G_kGCm'C Cuts at: 1790 3385 5064 6220 1790 Size: 1595 1679 1156 2573 Fragments arranged by size: 2573 1679 1595 1156 BmgBI CAC'GTC Cuts at: 1318 3871 4280 1318 Size: 2553 409 4041 Fragments arranged by size: 4041 2553 409 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3918 4734 5692 Size: 416 53 83 399 24 2501 816 958 Cuts at: 5692 442 Size: 1753 Fragments arranged by size: 2501 1753 958 816 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7003 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2573 2972 2996 5663 1239 Size: 1334 399 24 2667 2579 Fragments arranged by size: 2667 2579 1334 399 24 Bpu10I CC'TnA_GC Cuts at: 3177 3177 Size: 7003 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2104 2294 4213 5128 5993 6291 2104 Size: 190 1919 915 865 298 2816 Fragments arranged by size: 2816 1919 915 865 298 190 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3762 5666 882 Size: 617 2263 1904 2219 Fragments arranged by size: 2263 2219 1904 617 BsaAI yAC'GTr Cuts at: 493 1204 4794 493 Size: 711 3590 2702 Fragments arranged by size: 3590 2702 711 BsaBI GATnn'nnATC Cuts at: 6997 6997 Size: 7003 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3940 4810 5192 5827 Size: 53 83 186 936 2407 870 382 635 Cuts at: 5827 275 Size: 1451 Fragments arranged by size: 2407 1451 936 870 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5436 5834 6108 6255 6666 5436 Size: 398 274 147 411 5773 Fragments arranged by size: 5773 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2994 3024 3353 3383 3688 Size: 30 165 30 1591 30 329 30 305 Cuts at: 3688 3718 1178 Size: 30 4463 Fragments arranged by size: 4463 1591 329 305 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2564 3053 3758 3776 1186 Size: 1378 489 705 18 4413 Fragments arranged by size: 4413 1378 705 489 18 BseYI C'CCAG_C Cuts at: 2254 3547 3800 3891 6206 6702 2254 Size: 1293 253 91 2315 496 2555 Fragments arranged by size: 2555 2315 1293 496 253 91 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3113 3121 1163 Size: 573 1377 8 5045 Fragments arranged by size: 5045 1377 573 8 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4453 5214 6242 1540 Size: 275 186 2452 761 1028 2301 Fragments arranged by size: 2452 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3306 5064 5149 6220 3306 Size: 1758 85 1071 4089 Fragments arranged by size: 4089 1758 1071 85 BsmI GAATG_Cn' Cuts at: 2399 4514 4607 2399 Size: 2115 93 4795 Fragments arranged by size: 4795 2115 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2280 2692 2698 3306 3385 4322 5064 Size: 399 490 412 6 608 79 937 742 Cuts at: 5064 5149 6220 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 937 742 608 490 412 399 85 79 6 BspEI T'CCGG_A Cuts at: 6666 6666 Size: 7003 BspHI T'CATG_A Cuts at: 3202 4892 6622 3202 Size: 1690 1730 3583 Fragments arranged by size: 3583 1730 1690 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3481 4248 4358 844 Size: 2637 767 110 3489 Fragments arranged by size: 3489 2637 767 110 BsrBI CCG'CTC Cuts at: 1394 1823 4890 1394 Size: 429 3067 3507 Fragments arranged by size: 3507 3067 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2502 5498 5672 65 Size: 1183 1254 2996 174 1396 Fragments arranged by size: 2996 1396 1254 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5647 5834 1700 Size: 110 3837 187 2869 Fragments arranged by size: 3837 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7003 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7003 BssSI C'ACGA_G Cuts at: 5063 6075 5063 Size: 1012 5991 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7003 BstBI TT'CG_AA Cuts at: 5807 5807 Size: 7003 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7003 BstXI CCAn_nnnn'nTGG Cuts at: 2650 2875 2906 2650 Size: 225 31 6747 Fragments arranged by size: 6747 225 31 BstYI r'GATC_y Cuts at: 1972 2266 2382 2602 3296 4112 4333 4700 5085 Size: 294 116 220 694 816 221 367 385 Cuts at: 5085 5102 6554 6640 6652 6998 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 816 694 385 367 346 294 221 220 116 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3645 3963 3645 Size: 318 6685 BtgI C'CryG_G Cuts at: 513 1065 2619 513 Size: 552 1554 4897 Fragments arranged by size: 4897 1554 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6264 Fragments arranged by size: 6264 683 56 BtsI GCAGTG_nn' Cuts at: 4519 5313 5333 4519 Size: 794 20 6189 Fragments arranged by size: 6189 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7003 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2325 2360 3852 3887 529 Size: 35 1761 35 1492 35 3645 Fragments arranged by size: 3645 1761 1492 35 35 35 DraI TTT'AAA Cuts at: 4307 4307 Size: 7003 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3700 4244 6010 809 Size: 507 2384 544 1766 1802 Fragments arranged by size: 2384 1802 1766 544 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2301 2937 2982 Size: 54 1425 50 275 18 471 636 45 Cuts at: 2982 4450 5339 6968 8 Size: 1468 889 1629 43 Fragments arranged by size: 1629 1468 1425 889 636 471 275 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4450 1537 Size: 275 2638 4090 Fragments arranged by size: 4090 2638 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2649 3030 3528 3537 3753 3830 4933 1353 Size: 1296 381 498 9 216 77 1103 3423 Fragments arranged by size: 3423 1296 1103 498 381 216 77 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2191 5590 5964 6110 387 Size: 1064 138 602 3399 374 146 1280 Fragments arranged by size: 3399 1280 1064 602 374 146 138 EcoICRI GAG'CTC Cuts at: 3304 3304 Size: 7003 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2256 2468 2573 2666 2972 2996 5066 Size: 393 624 212 105 93 306 24 2070 Cuts at: 5066 5663 6450 6718 1239 Size: 597 787 268 1524 Fragments arranged by size: 2070 1524 787 624 597 393 306 268 212 105 93 24 EcoNI CCTnn'n_nnAGG Cuts at: 2486 2555 2654 2486 Size: 69 99 6835 Fragments arranged by size: 6835 99 69 EcoO109I rG'GnC_Cy Cuts at: 2419 2434 3172 3618 3968 2419 Size: 15 738 446 350 5454 Fragments arranged by size: 5454 738 446 350 15 EcoRI G'AATT_C Cuts at: 4312 4690 4312 Size: 378 6625 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2809 3083 2809 Size: 274 6729 FspI TGC'GCA Cuts at: 5509 5509 Size: 7003 HaeII r_GCGC'y Cuts at: 1678 6150 1678 Size: 4472 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2113 2145 3288 3320 3353 Size: 32 135 32 541 32 1143 32 33 Cuts at: 3353 3385 5644 5676 5718 5750 1373 Size: 32 2259 32 42 32 2626 Fragments arranged by size: 2626 2259 1143 541 135 42 33 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4347 4593 669 Size: 988 281 2409 246 3079 Fragments arranged by size: 3079 2409 988 281 246 HindIII A'AGCT_T Cuts at: 748 2135 3792 748 Size: 1387 1657 3959 Fragments arranged by size: 3959 1657 1387 HpaI GTT'AAC Cuts at: 4593 4593 Size: 7003 KpnI G_GTAC'C Cuts at: 4328 4328 Size: 7003 MfeI C'AATT_G Cuts at: 4602 4602 Size: 7003 MluI A'CGCG_T Cuts at: 6879 6879 Size: 7003 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2852 6117 6301 1305 Size: 1547 3265 184 2007 Fragments arranged by size: 3265 2007 1547 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2303 2939 2984 10 Size: 54 1425 343 471 636 45 4029 Fragments arranged by size: 4029 1425 636 471 343 54 45 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3053 4961 5320 5479 518 Size: 648 843 1044 1908 359 159 2042 Fragments arranged by size: 2042 1908 1044 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2470 4419 5096 6244 6489 1606 Size: 864 1949 677 1148 245 2120 Fragments arranged by size: 2120 1949 1148 864 677 245 NcoI C'CATG_G Cuts at: 513 1065 2619 513 Size: 552 1554 4897 Fragments arranged by size: 4897 1554 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7003 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7003 NotI GC'GGCC_GC Cuts at: 4450 4450 Size: 7003 NspI r_CATG'y Cuts at: 1132 1229 2522 4361 5906 1132 Size: 97 1293 1839 1545 2229 Fragments arranged by size: 2229 1839 1545 1293 97 PasI CC'CwG_GG Cuts at: 1348 2482 1348 Size: 1134 5869 PciI A'CATG_T Cuts at: 5902 5902 Size: 7003 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7003 PfoI T'CCnGG_A Cuts at: 3416 3416 Size: 7003 PmeI GTTT'AAAC Cuts at: 4307 4307 Size: 7003 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2508 2540 5072 5104 6585 6617 2508 Size: 32 2532 32 1481 32 2894 Fragments arranged by size: 2894 2532 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2419 2434 3172 3618 3968 2419 Size: 15 738 446 350 5454 Fragments arranged by size: 5454 738 446 350 15 PshAI GACnn'nnGTC Cuts at: 1613 3607 1613 Size: 1994 5009 PsiI TTA'TAA Cuts at: 4573 4573 Size: 7003 PstI C_TGCA'G Cuts at: 830 2703 4355 830 Size: 1873 1652 3478 Fragments arranged by size: 3478 1873 1652 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7003 PvuII CAG'CTG Cuts at: 2470 2470 Size: 7003 RsrII CG'GwC_CG Cuts at: 3110 3110 Size: 7003 SacI G_AGCT'C Cuts at: 3306 3306 Size: 7003 SalI G'TCGA_C Cuts at: 1936 4345 1936 Size: 2409 4594 SanDI GG'GwC_CC Cuts at: 2434 2434 Size: 7003 SapI GCTCTTCn'nnn_ Cuts at: 3528 3537 3528 Size: 9 6994 SbfI CC_TGCA'GG Cuts at: 4355 4355 Size: 7003 ScaI AGT'ACT Cuts at: 5251 6963 5251 Size: 1712 5291 SexAI A'CCwGG_T Cuts at: 2016 2016 Size: 7003 SfcI C'TryA_G Cuts at: 826 1045 2699 2813 3503 3680 4107 4351 5486 Size: 219 1654 114 690 177 427 244 1135 Cuts at: 5486 6167 6358 826 Size: 681 191 1471 Fragments arranged by size: 1654 1471 1135 690 681 427 244 219 191 177 114 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7003 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7003 SmaI CCC'GGG Cuts at: 1872 4330 1872 Size: 2458 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2083 2273 2891 4228 5107 6008 Size: 197 926 140 190 618 1337 879 901 Cuts at: 6008 6270 820 Size: 262 1553 Fragments arranged by size: 1553 1337 926 901 879 618 262 197 190 140 SnaBI TAC'GTA Cuts at: 493 4794 493 Size: 4301 2702 SpeI A'CTAG_T Cuts at: 152 2056 152 Size: 1904 5099 SphI G_CATG'C Cuts at: 1132 4361 1132 Size: 3229 3774 SspI AAT'ATT Cuts at: 5 52 4927 5 Size: 47 4875 2081 Fragments arranged by size: 4875 2081 47 StuI AGG'CCT Cuts at: 1517 2630 1517 Size: 1113 5890 StyI C'CwwG_G Cuts at: 513 1065 2619 2718 4158 4442 513 Size: 552 1554 99 1440 284 3074 Fragments arranged by size: 3074 1554 1440 552 284 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3127 4142 5030 5047 5200 5385 5830 Size: 1255 1547 1015 888 17 153 185 445 Cuts at: 5830 325 Size: 1498 Fragments arranged by size: 1547 1498 1255 1015 888 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3520 5249 6961 Size: 274 80 33 51 1443 1543 1729 1712 Cuts at: 6961 96 Size: 138 Fragments arranged by size: 1729 1712 1543 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2994 3026 3688 3720 6963 6995 2994 Size: 32 662 32 3243 32 3002 Fragments arranged by size: 3243 3002 662 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7003 XbaI T'CTAG_A Cuts at: 4339 4339 Size: 7003 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2947 2947 Size: 7003 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7003 XmaI C'CCGG_G Cuts at: 1870 4328 1870 Size: 2458 4545 XmnI GAAnn'nnTTC Cuts at: 5132 5132 Size: 7003 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4811 276 Size: 53 83 186 936 3277 2468 Fragments arranged by size: 3277 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI SacII SfiI SfoI SrfI SwaI