Restriction map for
pFN21AA0247
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BciVI BclI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BseAI BsgI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
HphI KasI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MseI MunI MvnI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp509I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AlwI 84 GATCC GGATC(4/5)
AlwNI 740 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 613 GGGCCC GGGCC^C
Asp700I 438 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 27 GAGCAC G[AT]GC[AT]^C
AspI 481 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 731 CTCGGG C^[CT]CG[GA]G
BcgI 200 GCAGTGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 170 CCCGG CC^[GC]GG
BfaI 79 CTAG C^TAG
BsaWI 135 ACCGGA [AT]^CCGG[AT]
BsiHKAI 27 GAGCAC G[AT]GC[AT]^C
BsoBI 731 CTCGGG C^[CT]CG[GA]G
BspLU11I 233 ACATGT A^CATGT
BsrFI 784 GCCGGC [GA]^CCGG[CT]
BssSI 403 CTCGTG CACGAG(-5/-1)
BstAPI 642 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 501 GGATG GGATG(2/0)
CfoI 388 GCGC GCG^C
Cfr10I 784 GCCGGC [GA]^CCGG[CT]
DpnII 84 GATC ^GATC
DraIII 559 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 782 TGGCCG [CT]^GGCC[GA]
EarI 771 CTCTTC CTCTTC(1/4)
FokI 501 GGATG GGATG(9/13)
HaeII 387 GGCGCT [GA]GCGC^[CT]
HhaI 388 GCGC GCG^C
HinP1I 388 GCGC G^CGC
Ksp632I 771 CTCTTC CTCTTC(1/4)
MaeI 79 CTAG C^TAG
MboI 84 GATC ^GATC
MspA1I 646 CAGCTG C[AC]G^C[GT]G
NaeI 784 GCCGGC GCC^GGC
NciI 170 CCCGG CC^[GC]GG
NdeII 84 GATC ^GATC
NgoMIV 784 GCCGGC G^CCGGC
NspI 233 ACATGT [GA]CATG^[CT]
PflFI 481 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 142 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 506 GAGTC GAGTC(4/5)
PpuMI 344 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 646 CAGCTG CAG^CTG
Sau3AI 84 GATC ^GATC
SexAI 482 ACCAGGT A^CC[AT]GGT
SfaNI 756 GCATC GCATC(5/9)
TaqI 209 TCGA T^CGA
TfiI 826 GATTC G^A[AT]TC
Tth111I 481 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 209 TCGA T^CGA
Van91I 142 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 438 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AciI 76 CCGC CCGC(-3/-1)
798 CCGC
AfaI 586 GTAC GT^AC
876 GTAC
AflIII 233 ACATGT A^C[GA][CT]GT
262 ACGTGT
BanII 613 GGGCCC G[GA]GC[CT]^C
726 GGGCTC
BsaI 182 GAGACC GGTCTC(1/5)
486 GGTCTC
BsmBI 307 CGTCTC CGTCTC(1/5)
104 GAGACG
BspMI 570 ACCTGC ACCTGC(4/8)
676 GCAGGT
BstXI 377 CCAGCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
491 CCATCATGGTGG
HinfI 506 GAGTC G^A[ACGT]TC
826 GATTC
MaeII 262 ACGT A^CGT
865 ACGT
MboII 685 GAAGA GAAGA(8/7)
772 TCTTC
MslI 30 CACCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
492 CATCATGGTG
NcoI 6 CCATGG C^CATGG
290 CCATGG
RsaI 586 GTAC GT^AC
876 GTAC
XcmI 330 CCACACATCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
465 CCAGGGGGTATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AluI AvaII BbvI BmyI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI Cac8I
Cfr13I DdeI DraII DsaI EcoO109I
EcoRII EcoT14I Fnu4HI HaeIII HapII
HpaII ItaI MaeIII MnlI MspI
MvaI MwoI NlaIII NlaIV PstI
Sau96I ScrFI SfcI StyI TseI
Tsp45I TspRI