Restriction map for
pFN21AA0185
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# NO CUT ENZYMES ( 60 enzymes) #
##################################
AccI AclI AgeI AscI AseI
AsnI AvrII BbrPI BclI BglII
BlnI BsaAI BsaBI BsiEI BsiWI
Bsp1407I BspDI BsrGI BssHII BssSI
Bst1107I BstZ17I ClaI CpoI DraI
EagI EclXI Eco52I EcoT22I FbaI
HpaI MamI MfeI MluI MunI
NheI NotI NruI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SalI
SgrAI SmaI SnaBI SpeI SspBI
SspI SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 5437 GACGTC GACGT^C
AccIII 1476 TCCGGA T^CCGGA
AflII 4936 CTTAAG C^TTAAG
Aor51HI 5507 AGCGCT AGC^GCT
ApaI 4931 GGGCCC GGGCC^C
AviII 2474 TGCGCA TGC^GCA
BbeI 5194 GGCGCC GGCGC^C
BfrI 4936 CTTAAG C^TTAAG
BseAI 1476 TCCGGA T^CCGGA
BspEI 1476 TCCGGA T^CCGGA
BspLU11I 5006 ACATGT A^CATGT
BstBI 3335 TTCGAA TT^CGAA
BstEII 2346 GGTGACC G^GT[ACGT]ACC
BstPI 2346 GGTGACC G^GT[ACGT]ACC
DrdI 3123 GACTGTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5507 AGCGCT AGC^GCT
EcoO65I 2346 GGTGACC G^GT[ACGT]ACC
EcoRI 3530 GAATTC G^AATTC
EcoRV 1337 GATATC GAT^ATC
FseI 3441 GGCCGGCC GGCCGG^CC
FspI 2474 TGCGCA TGC^GCA
KasI 5194 GGCGCC G^GCGCC
KspI 4811 CCGCGG CCGC^GG
MroI 1476 TCCGGA T^CCGGA
NarI 5194 GGCGCC GG^CGCC
NdeI 1394 CATATG CA^TATG
NspV 3335 TTCGAA TT^CGAA
SacII 4811 CCGCGG CCGC^GG
SapI 2208 GAAGAGC GCTCTTC(1/4)
ScaI 1723 AGTACT AGT^ACT
SfiI 3603 GGCCCTGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 5194 GGCGCC GGC^GCC
SfuI 3335 TTCGAA TT^CGAA
SphI 560 GCATGC GCATG^C
StuI 1226 AGGCCT AGG^CCT
XbaI 3204 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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Acc65I 574 GGTACC G^GTACC
1437 GGTACC
AhdI 842 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2918 GACAGGGTGTC
Alw44I 1021 GTGCAC G^TGCAC
2716 GTGCAC
ApaLI 1021 GTGCAC G^TGCAC
2716 GTGCAC
Asp718I 574 GGTACC G^GTACC
1437 GGTACC
AspEI 842 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2918 GACAGGGTGTC
AvaI 26 CCCGAG C^[CT]CG[GA]G
3269 CCCGAG
BcgI 1072 CGACTCTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4541 CGAATGTGCTGC
BciVI 580 GTATCC GTATCC(6/5)
3185 GTATCC
BglI 314 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3604 GCCCTGAGGGC
BsaI 4280 GAGACC GGTCTC(1/5)
2130 GGTCTC
BsaWI 1476 TCCGGA [AT]^CCGG[AT]
5144 ACCGGA
BsoBI 26 CCCGAG C^[CT]CG[GA]G
3269 CCCGAG
BspHI 1341 TCATGA T^CATGA
2279 TCATGA
BsrBI 5332 GAGCGG CCGCTC(-3/-3)
5455 GAGCGG
Eam1105I 842 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2918 GACAGGGTGTC
HincII 523 GTCAAC GT[CT]^[GA]AC
922 GTTGAC
HindII 523 GTCAAC GT[CT]^[GA]AC
922 GTTGAC
KpnI 574 GGTACC GGTAC^C
1437 GGTACC
PshAI 2401 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5031 GACCAAGGTC
PvuII 4594 CAGCTG CAG^CTG
5317 CAGCTG
RcaI 1341 TCATGA T^CATGA
2279 TCATGA
SbfI 1864 CCTGCAGG CCTGCA^GG
4887 CCTGCAGG
SexAI 2612 ACCTGGT A^CC[AT]GGT
4166 ACCTGGT
Sse8387I 1864 CCTGCAGG CCTGCA^GG
4887 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI Asp700I AspHI AspI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmnI