Restriction map for
pFN21AA0159
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# NO CUT ENZYMES ( 77 enzymes) #
##################################
AccII AccIII AclI AflII AgeI
Alw44I ApaLI AscI AviII AvrII
BbrPI BbvCI BfrI BglI BlnI
BsaAI BsaBI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII Bst1107I BstEII BstPI BstUI
BstZ17I ClaI CpoI DraI DraIII
EagI EclXI Eco52I EcoNI EcoO65I
EcoT22I FseI FspI HpaI KspI
MamI MfeI MluI MroI MunI
MvnI NheI NotI NruI NsiI
PacI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SgrAI
SnaBI SphI Sse8387I SspBI SspI
SwaI XbaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 1232 GACGTC GACGT^C
Aor51HI 2309 AGCGCT AGC^GCT
AseI 631 ATTAAT AT^TAAT
AsnI 631 ATTAAT AT^TAAT
AspI 4006 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2852 GGCGCC GGCGC^C
BclI 3137 TGATCA T^GATCA
BglII 4119 AGATCT A^GATCT
BlpI 3313 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3313 GCTCAGC GC^T[ACGT]AGC
BsaWI 3212 TCCGGT [AT]^CCGG[AT]
BsmBI 376 CGTCTC CGTCTC(1/5)
BsmI 904 GCATTC GAATGC(1/-1)
BspHI 3566 TCATGA T^CATGA
BsrFI 2849 GCCGGC [GA]^CCGG[CT]
BssSI 2345 CACGAG CACGAG(-5/-1)
BstBI 209 TTCGAA TT^CGAA
CelII 3313 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2849 GCCGGC [GA]^CCGG[CT]
DrdI 848 GACAAAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2309 AGCGCT AGC^GCT
EcoRV 3505 GATATC GAT^ATC
FbaI 3137 TGATCA T^GATCA
HgaI 1553 GACGC GACGC(5/10)
HincII 1126 GTCAAC GT[CT]^[GA]AC
HindII 1126 GTCAAC GT[CT]^[GA]AC
KasI 2852 GGCGCC G^GCGCC
NaeI 2849 GCCGGC GCC^GGC
NarI 2852 GGCGCC GG^CGCC
NdeI 706 CATATG CA^TATG
NgoMIV 2849 GCCGGC G^CCGGC
NspI 953 GCATGT [GA]CATG^[CT]
NspV 209 TTCGAA TT^CGAA
PaeR7I 1607 CTCGAG C^TCGAG
PflFI 4006 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 538 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 631 ATTAAT AT^TAAT
RcaI 3566 TCATGA T^CATGA
SfoI 2852 GGCGCC GGC^GCC
SfuI 209 TTCGAA TT^CGAA
SmaI 1745 CCCGGG CCC^GGG
SpeI 1839 ACTAGT A^CTAGT
Tth111I 4006 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1607 CTCGAG C^TCGAG
XmaCI 1745 CCCGGG C^CCGGG
XmaI 1745 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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Acc65I 2001 GGTACC G^GTACC
3971 GGTACC
AccI 1461 GTCTAC GT^[AC][GT]AC
1752 GTCTAC
AcyI 1232 GACGTC G[GA]^CG[CT]C
2852 GGCGCC
AflIII 927 ACGTGT A^C[GA][CT]GT
1198 ACACGT
AhdI 1775 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3754 GACAAACTGTC
Asp700I 2477 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3450 GAACTTCTTC
Asp718I 2001 GGTACC G^GTACC
3971 GGTACC
AspEI 1775 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3754 GACAAACTGTC
BamHI 1809 GGATCC G^GATCC
2591 GGATCC
BcgI 201 GCATCACTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3858 GCAGAAGCTTCG
BciVI 1098 GGATAC GTATCC(6/5)
265 GTATCC
BsaHI 1232 GACGTC G[GA]^CG[CT]C
2852 GGCGCC
BsgI 1957 GTGCAG GTGCAG(16/14)
2227 GTGCAG
BsrBI 2272 GAGCGG CCGCTC(-3/-3)
3658 GAGCGG
BsrDI 2454 CATTGC GCAATG(2/0)
2746 GCAATG
BstXI 3260 CCAATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3881 CCAGAGGTTTGG
Eam1105I 1775 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3754 GACAAACTGTC
EcoRI 247 GAATTC G^AATTC
1972 GAATTC
Hin1I 1232 GACGTC G[GA]^CG[CT]C
2852 GGCGCC
HindIII 1318 AAGCTT A^AGCTT
3862 AAGCTT
KpnI 2001 GGTACC GGTAC^C
3971 GGTACC
PpuMI 2726 AGGACCC [GA]G^G[AT]CC[CT]
3305 GGGACCC
StuI 3075 AGGCCT AGG^CCT
3714 AGGCCT
XmnI 2477 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3450 GAACTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII