(Circular) (Six-base) MAPSORT of: pFN21AA0159.seq Check: 8964 from: 1 to: 8908 pFN21AA0159 8908 bp With 182 enzymes: SgfI * August 20, 2012 11:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2688 4435 2688 Size: 1747 7161 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3241 6718 278 Size: 53 83 186 936 1705 3477 2468 Fragments arranged by size: 3477 2468 1705 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3467 3758 6251 1937 Size: 1530 291 2493 4594 Fragments arranged by size: 4594 2493 1530 291 Acc65I G'GTAC_C Cuts at: 4006 5976 6229 4006 Size: 1970 253 6685 Fragments arranged by size: 6685 1970 253 AclI AA'CG_TT Cuts at: 1563 7035 7408 1563 Size: 5472 373 3063 Fragments arranged by size: 5472 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2498 2519 2871 3564 3634 5767 6971 8355 Size: 866 21 352 693 70 2133 1204 1384 Cuts at: 8355 8623 1632 Size: 268 1917 Fragments arranged by size: 2133 1917 1384 1204 866 693 352 268 70 21 AfeI AGC'GCT Cuts at: 4316 4316 Size: 8908 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8711 AflIII A'CryG_T Cuts at: 1203 2932 3203 7807 8784 1203 Size: 1729 271 4604 977 1327 Fragments arranged by size: 4604 1729 1327 977 271 AgeI A'CCGG_T Cuts at: 7739 7739 Size: 8908 AhdI GACnn_n'nnGTC Cuts at: 3785 5764 7637 3785 Size: 1979 1873 5056 Fragments arranged by size: 5056 1979 1873 AleI CACnn'nnGTG Cuts at: 1166 4391 1166 Size: 3225 5683 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4698 1184 Size: 3514 5394 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4769 4801 4769 Size: 32 8876 AlwNI CAG_nnn'CTG Cuts at: 2746 3286 3413 3874 4018 4270 4696 4816 5035 Size: 540 127 461 144 252 426 120 219 Cuts at: 5035 5986 6651 8223 2746 Size: 951 665 1572 3431 Fragments arranged by size: 3431 1572 951 665 540 461 426 252 219 144 127 120 ApaI G_GGCC'C Cuts at: 2163 3391 4649 2163 Size: 1228 1258 6422 Fragments arranged by size: 6422 1258 1228 ApaLI G'TGCA_C Cuts at: 6965 8121 6965 Size: 1156 7752 ApoI r'AATT_y Cuts at: 1493 2252 2582 3977 4215 5855 6217 6443 6595 Size: 759 330 1395 238 1640 362 226 152 Cuts at: 6595 8704 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1640 1395 759 362 330 238 226 152 AseI AT'TA_AT Cuts at: 160 1032 2637 7462 160 Size: 872 1605 4825 1606 Fragments arranged by size: 4825 1606 1605 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8908 AvaI C'yCGr_G Cuts at: 1870 1943 2661 3078 3612 3750 5426 5534 6233 Size: 73 718 417 534 138 1676 108 699 Cuts at: 6233 1870 Size: 4545 Fragments arranged by size: 4545 1676 718 699 534 417 138 108 73 BamHI G'GATC_C Cuts at: 3814 4596 6238 6605 3814 Size: 782 1642 367 6117 Fragments arranged by size: 6117 1642 782 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2767 4006 4857 5976 6229 Size: 325 217 627 980 1239 851 1119 253 Cuts at: 6229 7684 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1239 1119 980 851 627 325 253 217 BanII G_rGCy'C Cuts at: 1391 2155 2163 3391 4649 4851 4922 5472 6030 Size: 764 8 1228 1258 202 71 550 558 Cuts at: 6030 6227 1391 Size: 197 4072 Fragments arranged by size: 4072 1258 1228 764 558 550 202 197 71 8 BbeI G_GCGC'C Cuts at: 4861 4861 Size: 8908 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3985 5168 6088 6153 928 Size: 971 2086 1183 920 65 3683 Fragments arranged by size: 3683 2086 1183 971 920 65 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2195 2229 5852 5886 7099 7133 1903 Size: 34 258 34 3623 34 1213 34 3678 Fragments arranged by size: 3678 3623 1213 258 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2281 3097 6800 8010 2281 Size: 816 3703 1210 3179 Fragments arranged by size: 3703 3179 1210 816 BclI T'GATC_A Cuts at: 1548 5142 1548 Size: 3594 5314 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7519 136 Size: 107 122 71 631 605 5847 1525 Fragments arranged by size: 5847 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6124 8903 6124 Size: 2779 6129 BlpI GC'TnA_GC Cuts at: 5319 6325 5319 Size: 1006 7902 Bme1580I G_kGCm'C Cuts at: 1790 2163 3391 4649 4666 6969 8125 1790 Size: 373 1228 1258 17 2303 1156 2573 Fragments arranged by size: 2573 2303 1258 1228 1156 373 17 BmgBI CAC'GTC Cuts at: 1318 5819 1318 Size: 4501 4407 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2497 5250 6639 Size: 416 53 83 399 24 1080 2753 1389 Cuts at: 6639 7597 442 Size: 958 1753 Fragments arranged by size: 2753 1753 1389 1080 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8908 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2642 4331 4718 7568 1239 Size: 1403 1689 387 2850 2579 Fragments arranged by size: 2850 2579 1689 1403 387 Bpu10I CC'TnA_GC Cuts at: 2154 3516 2154 Size: 1362 7546 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4229 5351 5933 6085 7033 7898 8196 4229 Size: 1122 582 152 948 865 298 4941 Fragments arranged by size: 4941 1122 948 865 582 298 152 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2393 2874 3084 3356 3674 4919 5670 Size: 617 894 481 210 272 318 1245 751 Cuts at: 5670 7571 882 Size: 1901 2219 Fragments arranged by size: 2219 1901 1245 894 751 617 481 318 272 210 BsaAI yAC'GTr Cuts at: 493 1204 6699 493 Size: 711 5495 2702 Fragments arranged by size: 5495 2702 711 BsaBI GATnn'nnATC Cuts at: 8902 8902 Size: 8908 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3238 4858 6715 7097 Size: 53 83 186 936 1705 1620 1857 382 Cuts at: 7097 7732 275 Size: 635 1451 Fragments arranged by size: 1857 1705 1620 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5217 7341 7739 8013 8160 8571 5217 Size: 2124 398 274 147 411 5554 Fragments arranged by size: 5554 2124 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2823 2853 3878 3908 1178 Size: 30 165 30 1420 30 1025 30 6178 Fragments arranged by size: 6178 1420 1025 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 3446 3449 3518 3521 3683 3692 4952 Size: 877 1383 3 69 3 162 9 1260 Cuts at: 4952 4955 5277 5573 1186 Size: 3 322 296 4521 Fragments arranged by size: 4521 1383 1260 877 322 296 162 69 9 3 3 3 BseYI C'CCAG_C Cuts at: 2938 3721 4263 4440 4793 4945 5166 5539 6060 Size: 783 542 177 353 152 221 373 521 Cuts at: 6060 8111 8607 2938 Size: 2051 496 3239 Fragments arranged by size: 3239 2051 783 542 521 496 373 353 221 177 152 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3983 4253 1163 Size: 573 2247 270 5818 Fragments arranged by size: 5818 2247 573 270 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6358 7119 8147 1540 Size: 275 186 4357 761 1028 2301 Fragments arranged by size: 4357 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2155 4851 4887 4922 5472 6969 7054 8125 2155 Size: 2696 36 35 550 1497 85 1071 2938 Fragments arranged by size: 2938 2696 1497 1071 550 85 36 35 BsmI GAATG_Cn' Cuts at: 2909 6419 6512 2909 Size: 3510 93 5305 Fragments arranged by size: 5305 3510 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2387 2387 Size: 8908 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2155 2163 3391 4649 4666 4851 4887 Size: 399 365 8 1228 1258 17 185 36 Cuts at: 4887 4922 5472 6030 6227 6969 7054 8125 1391 Size: 35 550 558 197 742 85 1071 2174 Fragments arranged by size: 2174 1258 1228 1071 742 558 550 399 365 197 185 85 36 35 17 8 BspEI T'CCGG_A Cuts at: 8571 8571 Size: 8908 BspHI T'CATG_A Cuts at: 5571 6797 8527 5571 Size: 1226 1730 5952 Fragments arranged by size: 5952 1730 1226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2688 3034 4435 4788 5383 5918 6263 844 Size: 1844 346 1401 353 595 535 345 3489 Fragments arranged by size: 3489 1844 1401 595 535 353 346 345 BsrBI CCG'CTC Cuts at: 1394 1823 4279 5665 6795 1394 Size: 429 2456 1386 1130 3507 Fragments arranged by size: 3507 2456 1386 1130 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4458 4758 7403 7577 65 Size: 1183 3210 300 2645 174 1396 Fragments arranged by size: 3210 2645 1396 1183 300 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4854 7552 7739 1700 Size: 110 3044 2698 187 2869 Fragments arranged by size: 3044 2869 2698 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8908 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8908 BssSI C'ACGA_G Cuts at: 4350 6968 7980 4350 Size: 2618 1012 5278 Fragments arranged by size: 5278 2618 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3413 3859 5136 794 Size: 2619 446 1277 4566 Fragments arranged by size: 4566 2619 1277 446 BstBI TT'CG_AA Cuts at: 2215 7712 2215 Size: 5497 3411 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8908 BstXI CCAn_nnnn'nTGG Cuts at: 5272 5893 5272 Size: 621 8287 BstYI r'GATC_y Cuts at: 1972 2224 3814 4596 4897 5245 6124 6203 6238 Size: 252 1590 782 301 348 879 79 35 Cuts at: 6238 6605 6990 7007 8459 8545 8557 8903 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1590 1452 879 782 385 367 348 346 301 252 86 79 35 17 12 Bsu36I CC'TnA_GG Cuts at: 3819 4524 5832 3819 Size: 705 1308 6895 Fragments arranged by size: 6895 1308 705 BtgI C'CryG_G Cuts at: 513 1065 2004 2403 2476 3024 3118 4932 4993 Size: 552 939 399 73 548 94 1814 61 Cuts at: 4993 513 Size: 4428 Fragments arranged by size: 4428 1814 939 552 548 399 94 73 61 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8169 Fragments arranged by size: 8169 683 56 BtsI GCAGTG_nn' Cuts at: 3222 3350 3423 4263 4623 4842 6424 7218 7238 Size: 128 73 840 360 219 1582 794 20 Cuts at: 7238 3222 Size: 4892 Fragments arranged by size: 4892 1582 840 794 360 219 128 73 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8908 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2406 2441 3939 3974 529 Size: 35 1842 35 1498 35 5463 Fragments arranged by size: 5463 1842 1498 35 35 35 DraI TTT'AAA Cuts at: 6212 6212 Size: 8908 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2859 7915 809 Size: 507 1543 5056 1802 Fragments arranged by size: 5056 1802 1543 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2406 3121 3288 Size: 54 1425 50 275 18 576 715 167 Cuts at: 3288 5250 5448 5920 6355 7244 8873 8 Size: 1962 198 472 435 889 1629 43 Fragments arranged by size: 1962 1629 1425 889 715 576 472 435 275 198 167 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6355 1537 Size: 275 4543 4090 Fragments arranged by size: 4543 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2490 2813 3170 4704 4709 5547 6838 1353 Size: 1137 323 357 1534 5 838 1291 3423 Fragments arranged by size: 3423 1534 1291 1137 838 357 323 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3044 7495 7869 8015 387 Size: 1064 138 1455 4451 374 146 1280 Fragments arranged by size: 4451 1455 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2153 4849 4920 5470 2153 Size: 2696 71 550 5591 Fragments arranged by size: 5591 2696 550 71 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2498 2519 2642 2871 3564 3634 4331 Size: 393 866 21 123 229 693 70 697 Cuts at: 4331 4718 5767 6971 7568 8355 8623 1239 Size: 387 1049 1204 597 787 268 1524 Fragments arranged by size: 1524 1204 1049 866 787 697 693 597 393 387 268 229 123 70 21 EcoO109I rG'GnC_Cy Cuts at: 2159 2160 3387 4015 4645 4646 4693 4732 5311 Size: 1 1227 628 630 1 47 39 579 Cuts at: 5311 2159 Size: 5756 Fragments arranged by size: 5756 1227 630 628 579 47 39 1 1 EcoRI G'AATT_C Cuts at: 2252 3977 6217 6595 2252 Size: 1725 2240 378 4565 Fragments arranged by size: 4565 2240 1725 378 EcoRV GAT'ATC Cuts at: 5512 5512 Size: 8908 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4100 5476 4100 Size: 1376 7532 FspI TGC'GCA Cuts at: 7414 7414 Size: 8908 HaeII r_GCGC'y Cuts at: 1678 4318 4861 5945 8055 1678 Size: 2640 543 1084 2110 2531 Fragments arranged by size: 2640 2531 2110 1084 543 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2084 2116 3119 3151 3449 Size: 32 135 32 512 32 1003 32 298 Cuts at: 3449 3481 3771 3803 4144 4176 4708 4740 4882 Size: 32 290 32 341 32 532 32 142 Cuts at: 4882 4914 5275 5307 5750 5782 7549 7581 7623 Size: 32 361 32 443 32 1767 32 42 Cuts at: 7623 7655 1373 Size: 32 2626 Fragments arranged by size: 2626 1767 1003 532 512 443 361 341 298 290 142 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3133 6252 6498 669 Size: 988 281 1195 3119 246 3079 Fragments arranged by size: 3119 3079 1195 988 281 246 HindIII A'AGCT_T Cuts at: 748 3323 5867 748 Size: 2575 2544 3789 Fragments arranged by size: 3789 2575 2544 HpaI GTT'AAC Cuts at: 6498 6498 Size: 8908 KasI G'GCGC_C Cuts at: 4857 4857 Size: 8908 KpnI G_GTAC'C Cuts at: 4010 5980 6233 4010 Size: 1970 253 6685 Fragments arranged by size: 6685 1970 253 MfeI C'AATT_G Cuts at: 6507 6507 Size: 8908 MluI A'CGCG_T Cuts at: 8784 8784 Size: 8908 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2520 3792 4998 8022 8206 1305 Size: 1215 1272 1206 3024 184 2007 Fragments arranged by size: 3024 2007 1272 1215 1206 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2408 3123 3290 5252 5450 Size: 54 1425 343 576 715 167 1962 198 Cuts at: 5450 5922 10 Size: 472 2996 Fragments arranged by size: 2996 1962 1425 715 576 472 343 198 167 54 MslI CAynn'nnrTG Cuts at: 518 1166 2346 2741 2982 3123 4391 5295 5570 Size: 648 1180 395 241 141 1268 904 275 Cuts at: 5570 6866 7225 7384 518 Size: 1296 359 159 2042 Fragments arranged by size: 2042 1296 1268 1180 904 648 395 359 275 241 159 141 MspA1I CmG'CkG Cuts at: 1606 3283 4267 4816 5111 5339 5641 5701 6324 Size: 1677 984 549 295 228 302 60 623 Cuts at: 6324 7001 8149 8394 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1677 1148 984 677 623 549 302 295 245 228 60 NaeI GCC'GGC Cuts at: 4856 4856 Size: 8908 NarI GG'CG_CC Cuts at: 4858 4858 Size: 8908 NcoI C'CATG_G Cuts at: 513 1065 2004 2403 2476 3024 3118 4932 513 Size: 552 939 399 73 548 94 1814 4489 Fragments arranged by size: 4489 1814 939 552 548 399 94 73 NdeI CA'TA_TG Cuts at: 387 2712 387 Size: 2325 6583 NgoMIV G'CCGG_C Cuts at: 4854 4854 Size: 8908 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8908 NotI GC'GGCC_GC Cuts at: 6355 6355 Size: 8908 NspI r_CATG'y Cuts at: 1132 1229 2962 6266 7811 1132 Size: 97 1733 3304 1545 2229 Fragments arranged by size: 3304 2229 1733 1545 97 PasI CC'CwG_GG Cuts at: 1348 3496 1348 Size: 2148 6760 PciI A'CATG_T Cuts at: 7807 7807 Size: 8908 PflMI CCAn_nnn'nTGG Cuts at: 1108 3100 3217 3286 5966 5986 1108 Size: 1992 117 69 2680 20 4030 Fragments arranged by size: 4030 2680 1992 117 69 20 PfoI T'CCnGG_A Cuts at: 2301 2385 3633 3896 4071 4869 5190 5307 2301 Size: 84 1248 263 175 798 321 117 5902 Fragments arranged by size: 5902 1248 798 321 263 175 117 84 PmeI GTTT'AAAC Cuts at: 6212 6212 Size: 8908 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6977 7009 8490 8522 6977 Size: 32 1481 32 7363 Fragments arranged by size: 7363 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4732 5311 4732 Size: 579 8329 PshAI GACnn'nnGTC Cuts at: 1613 2547 1613 Size: 934 7974 PsiI TTA'TAA Cuts at: 2706 6478 2706 Size: 3772 5136 PspOMI G'GGCC_C Cuts at: 2159 3387 4645 2159 Size: 1228 1258 6422 Fragments arranged by size: 6422 1258 1228 PstI C_TGCA'G Cuts at: 830 2178 2751 3045 3357 3406 3418 3477 3879 Size: 1348 573 294 312 49 12 59 402 Cuts at: 3879 4812 5110 6260 830 Size: 933 298 1150 3478 Fragments arranged by size: 3478 1348 1150 933 573 402 312 298 294 59 49 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8908 PvuII CAG'CTG Cuts at: 3283 4267 4816 5111 5701 3283 Size: 984 549 295 590 6490 Fragments arranged by size: 6490 984 590 549 295 SacI G_AGCT'C Cuts at: 2155 4851 4922 5472 2155 Size: 2696 71 550 5591 Fragments arranged by size: 5591 2696 550 71 SalI G'TCGA_C Cuts at: 1936 6250 1936 Size: 4314 4594 SanDI GG'GwC_CC Cuts at: 5311 5311 Size: 8908 SapI GCTCTTCn'nnn_ Cuts at: 2813 3170 5547 2813 Size: 357 2377 6174 Fragments arranged by size: 6174 2377 357 SbfI CC_TGCA'GG Cuts at: 6260 6260 Size: 8908 ScaI AGT'ACT Cuts at: 7156 8868 7156 Size: 1712 7196 SfcI C'TryA_G Cuts at: 826 1045 2174 2194 2724 2747 3041 3353 3402 Size: 219 1129 20 530 23 294 312 49 Cuts at: 3402 3414 3473 3485 3759 3875 3886 3954 4429 Size: 12 59 12 274 116 11 68 475 Cuts at: 4429 4808 5086 5106 5994 6256 7391 8072 8263 Size: 379 278 20 888 262 1135 681 191 Cuts at: 8263 826 Size: 1471 Fragments arranged by size: 1471 1135 1129 888 681 530 475 379 312 294 278 274 262 219 191 116 68 59 49 23 20 20 12 12 11 SfoI GGC'GCC Cuts at: 4859 4859 Size: 8908 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8908 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8908 SmaI CCC'GGG Cuts at: 1872 3752 6235 1872 Size: 1880 2483 4545 Fragments arranged by size: 4545 2483 1880 SmlI C'TyrA_G Cuts at: 820 1017 1943 3612 4208 5366 5912 6100 7012 Size: 197 926 1669 596 1158 546 188 912 Cuts at: 7012 7913 8175 820 Size: 901 262 1553 Fragments arranged by size: 1669 1553 1158 926 912 901 596 546 262 197 188 SnaBI TAC'GTA Cuts at: 493 6699 493 Size: 6206 2702 SpeI A'CTAG_T Cuts at: 152 3844 152 Size: 3692 5216 SphI G_CATG'C Cuts at: 1132 6266 1132 Size: 5134 3774 SspI AAT'ATT Cuts at: 5 52 6832 5 Size: 47 6780 2081 Fragments arranged by size: 6780 2081 47 StuI AGG'CCT Cuts at: 1517 5082 5721 1517 Size: 3565 639 4704 Fragments arranged by size: 4704 3565 639 StyI C'CwwG_G Cuts at: 513 1065 2004 2403 2437 2466 2476 3024 3118 Size: 552 939 399 34 29 10 548 94 Cuts at: 3118 4137 4902 4932 5125 5598 6347 513 Size: 1019 765 30 193 473 749 3074 Fragments arranged by size: 3074 1019 939 765 749 552 548 473 399 193 94 34 30 29 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4596 6074 6172 6935 6952 7105 7290 Size: 1255 3016 1478 98 763 17 153 185 Cuts at: 7290 7735 325 Size: 445 1498 Fragments arranged by size: 3016 1498 1478 1255 763 445 185 153 98 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2773 7154 8866 Size: 274 80 33 51 1443 796 4381 1712 Cuts at: 8866 96 Size: 138 Fragments arranged by size: 4381 1712 1443 796 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4581 4613 5618 5650 8868 8900 4581 Size: 32 1005 32 3218 32 4589 Fragments arranged by size: 4589 3218 1005 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 6014 1316 Size: 4698 4210 XbaI T'CTAG_A Cuts at: 6244 6244 Size: 8908 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3031 3283 3435 4019 4590 5245 3031 Size: 252 152 584 571 655 6694 Fragments arranged by size: 6694 655 584 571 252 152 XhoI C'TCGA_G Cuts at: 1943 3612 1943 Size: 1669 7239 XmaI C'CCGG_G Cuts at: 1870 3750 6233 1870 Size: 1880 2483 4545 Fragments arranged by size: 4545 2483 1880 XmnI GAAnn'nnTTC Cuts at: 4486 5459 7037 4486 Size: 973 1578 6357 Fragments arranged by size: 6357 1578 973 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3239 6716 276 Size: 53 83 186 936 1705 3477 2468 Fragments arranged by size: 3477 2468 1705 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoNI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI