Restriction map for
pFN21AA0159
(Linear) (Six-base) MAPSORT of: KIAA0159.seq Check: 731 from: 1 to: 4203
KIAA0159.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 683 2430 4203
Size: 683 1747 1773
Fragments arranged by size:
1773 1747 683
AatII G_ACGT'C
Cuts at: 0 1236 4203
Size: 1236 2967
AccI GT'mk_AC
Cuts at: 0 1462 1753 4203
Size: 1462 291 2450
Fragments arranged by size:
2450 1462 291
Acc65I G'GTAC_C
Cuts at: 0 2001 3971 4203
Size: 2001 1970 232
Fragments arranged by size:
2001 1970 232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 514 866 1559 1629 3762 4203
Size: 493 21 352 693 70 2133 441
Fragments arranged by size:
2133 693 493 441 352 70 21
AfeI AGC'GCT
Cuts at: 0 2311 4203
Size: 2311 1892
AflIII A'CryG_T
Cuts at: 0 927 1198 4203
Size: 927 271 3005
Fragments arranged by size:
3005 927 271
AhdI GACnn_n'nnGTC
Cuts at: 0 1780 3759 4203
Size: 1780 1979 444
Fragments arranged by size:
1979 1780 444
AleI CACnn'nnGTG
Cuts at: 0 2386 4203
Size: 2386 1817
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2693 4203
Size: 2693 1510
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2764 2796 4203
Size: 2764 32 1407
Fragments arranged by size:
2764 1407 32
AlwNI CAG_nnn'CTG
Cuts at: 0 741 1281 1408 1869 2013 2265 2691 2811
Size: 741 540 127 461 144 252 426 120
Cuts at: 2811 3030 3981 4203
Size: 219 951 222
Fragments arranged by size:
951 741 540 461 426 252 222 219
144 127 120
ApaI G_GGCC'C
Cuts at: 0 158 1386 2644 4203
Size: 158 1228 1258 1559
Fragments arranged by size:
1559 1258 1228 158
ApoI r'AATT_y
Cuts at: 0 247 577 1972 2210 3850 4203
Size: 247 330 1395 238 1640 353
Fragments arranged by size:
1640 1395 353 330 247 238
AseI AT'TA_AT
Cuts at: 0 632 4203
Size: 632 3571
AvaI C'yCGr_G
Cuts at: 0 656 1073 1607 1745 3421 3529 4203
Size: 656 417 534 138 1676 108 674
Fragments arranged by size:
1676 674 656 534 417 138 108
BamHI G'GATC_C
Cuts at: 0 1809 2591 4203
Size: 1809 782 1612
Fragments arranged by size:
1809 1612 782
BanI G'GyrC_C
Cuts at: 0 762 2001 2852 3971 4203
Size: 762 1239 851 1119 232
Fragments arranged by size:
1239 1119 851 762 232
BanII G_rGCy'C
Cuts at: 0 150 158 1386 2644 2846 2917 3467 4025
Size: 150 8 1228 1258 202 71 550 558
Cuts at: 4025 4203
Size: 178
Fragments arranged by size:
1258 1228 558 550 202 178 150 71
8
BbeI G_GCGC'C
Cuts at: 0 2856 4203
Size: 2856 1347
BbsI GAAGACnn'nnnn_
Cuts at: 0 1980 3163 4083 4148 4203
Size: 1980 1183 920 65 55
Fragments arranged by size:
1980 1183 920 65 55
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 190 224 3847 3881 4203
Size: 190 34 3623 34 322
Fragments arranged by size:
3623 322 190 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 276 1092 4203
Size: 276 816 3111
Fragments arranged by size:
3111 816 276
BclI T'GATC_A
Cuts at: 0 3137 4203
Size: 3137 1066
BglII A'GATC_T
Cuts at: 0 4119 4203
Size: 4119 84
BlpI GC'TnA_GC
Cuts at: 0 3314 4203
Size: 3314 889
Bme1580I G_kGCm'C
Cuts at: 0 158 1386 2644 2661 4203
Size: 158 1228 1258 17 1542
Fragments arranged by size:
1542 1258 1228 158 17
BmgBI CAC'GTC
Cuts at: 0 3814 4203
Size: 3814 389
BmrI ACTGGGnnnn_n'
Cuts at: 0 492 3245 4203
Size: 492 2753 958
Fragments arranged by size:
2753 958 492
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 2326 2713 4203
Size: 637 1689 387 1490
Fragments arranged by size:
1689 1490 637 387
Bpu10I CC'TnA_GC
Cuts at: 0 149 1511 4203
Size: 149 1362 2692
Fragments arranged by size:
2692 1362 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2224 3346 3928 4080 4203
Size: 2224 1122 582 152 123
Fragments arranged by size:
2224 1122 582 152 123
BsaI GGTCTCn'nnnn_
Cuts at: 0 388 869 1079 1351 1669 2914 3665 4203
Size: 388 481 210 272 318 1245 751 538
Fragments arranged by size:
1245 751 538 481 388 318 272 210
BsaHI Gr'CG_yC
Cuts at: 0 1233 2853 4203
Size: 1233 1620 1350
Fragments arranged by size:
1620 1350 1233
BsaWI w'CCGG_w
Cuts at: 0 3212 4203
Size: 3212 991
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 818 848 1873 1903 4203
Size: 818 30 1025 30 2300
Fragments arranged by size:
2300 1025 818 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 1441 1444 1513 1516 1678 1687 2947
Size: 58 1383 3 69 3 162 9 1260
Cuts at: 2947 2950 3272 3568 4203
Size: 3 322 296 635
Fragments arranged by size:
1383 1260 635 322 296 162 69 58
9 3 3 3
BseYI C'CCAG_C
Cuts at: 0 933 1716 2258 2435 2788 2940 3161 3534
Size: 933 783 542 177 353 152 221 373
Cuts at: 3534 4055 4203
Size: 521 148
Fragments arranged by size:
933 783 542 521 373 353 221 177
152 148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1978 2248 4203
Size: 1978 270 1955
Fragments arranged by size:
1978 1955 270
BsiHKAI G_wGCw'C
Cuts at: 0 150 2846 2882 2917 3467 4203
Size: 150 2696 36 35 550 736
Fragments arranged by size:
2696 736 550 150 36 35
BsmI GAATG_Cn'
Cuts at: 0 904 4203
Size: 904 3299
BsmBI CGTCTCn'nnnn_
Cuts at: 0 382 4203
Size: 382 3821
Bsp1286I G_dGCh'C
Cuts at: 0 150 158 1386 2644 2661 2846 2882 2917
Size: 150 8 1228 1258 17 185 36 35
Cuts at: 2917 3467 4025 4203
Size: 550 558 178
Fragments arranged by size:
1258 1228 558 550 185 178 150 36
35 17 8
BspHI T'CATG_A
Cuts at: 0 3566 4203
Size: 3566 637
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 683 1029 2430 2783 3378 3913 4203
Size: 683 346 1401 353 595 535 290
Fragments arranged by size:
1401 683 595 535 353 346 290
BsrBI CCG'CTC
Cuts at: 0 2274 3660 4203
Size: 2274 1386 543
Fragments arranged by size:
2274 1386 543
BsrDI GCAATG_nn'
Cuts at: 0 2453 2753 4203
Size: 2453 300 1450
Fragments arranged by size:
2453 1450 300
BsrFI r'CCGG_y
Cuts at: 0 2849 4203
Size: 2849 1354
BssSI C'ACGA_G
Cuts at: 0 2345 4203
Size: 2345 1858
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1408 1854 3131 4203
Size: 1408 446 1277 1072
Fragments arranged by size:
1408 1277 1072 446
BstBI TT'CG_AA
Cuts at: 0 210 4203
Size: 210 3993
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3267 3888 4203
Size: 3267 621 315
Fragments arranged by size:
3267 621 315
BstYI r'GATC_y
Cuts at: 0 219 1809 2591 2892 3240 4119 4198 4203
Size: 219 1590 782 301 348 879 79 5
Fragments arranged by size:
1590 879 782 348 301 219 79 5
Bsu36I CC'TnA_GG
Cuts at: 0 1814 2519 3827 4203
Size: 1814 705 1308 376
Fragments arranged by size:
1814 1308 705 376
BtgI C'CryG_G
Cuts at: 0 398 471 1019 1113 2927 2988 4203
Size: 398 73 548 94 1814 61 1215
Fragments arranged by size:
1814 1215 548 398 94 73 61
BtsI GCAGTG_nn'
Cuts at: 0 1217 1345 1418 2258 2618 2837 4203
Size: 1217 128 73 840 360 219 1366
Fragments arranged by size:
1366 1217 840 360 219 128 73
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 401 436 1934 1969 4203
Size: 401 35 1498 35 2234
Fragments arranged by size:
2234 1498 401 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 854 4203
Size: 854 3349
EaeI y'GGCC_r
Cuts at: 0 401 1116 1283 3245 3443 3915 4203
Size: 401 715 167 1962 198 472 288
Fragments arranged by size:
1962 715 472 401 288 198 167
EarI CTCTTCn'nnn_
Cuts at: 0 485 808 1165 2699 2704 3542 4203
Size: 485 323 357 1534 5 838 661
Fragments arranged by size:
1534 838 661 485 357 323 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1039 4203
Size: 1039 3164
EcoICRI GAG'CTC
Cuts at: 0 148 2844 2915 3465 4203
Size: 148 2696 71 550 738
Fragments arranged by size:
2696 738 550 148 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 514 637 866 1559 1629 2326 2713
Size: 493 21 123 229 693 70 697 387
Cuts at: 2713 3762 4203
Size: 1049 441
Fragments arranged by size:
1049 697 693 493 441 387 229 123
70 21
EcoO109I rG'GnC_Cy
Cuts at: 0 154 155 1382 2010 2640 2641 2688 2727
Size: 154 1 1227 628 630 1 47 39
Cuts at: 2727 3306 4203
Size: 579 897
Fragments arranged by size:
1227 897 630 628 579 154 47 39
1 1
EcoRI G'AATT_C
Cuts at: 0 247 1972 4203
Size: 247 1725 2231
Fragments arranged by size:
2231 1725 247
EcoRV GAT'ATC
Cuts at: 0 3507 4203
Size: 3507 696
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2095 3471 4203
Size: 2095 1376 732
Fragments arranged by size:
2095 1376 732
HaeII r_GCGC'y
Cuts at: 0 2313 2856 3940 4203
Size: 2313 543 1084 263
Fragments arranged by size:
2313 1084 543 263
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 79 111 1114 1146 1444 1476 1766 1798
Size: 79 32 1003 32 298 32 290 32
Cuts at: 1798 2139 2171 2703 2735 2877 2909 3270 3302
Size: 341 32 532 32 142 32 361 32
Cuts at: 3302 3745 3777 4203
Size: 443 32 426
Fragments arranged by size:
1003 532 443 426 361 341 298 290
142 79 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1128 4203
Size: 1128 3075
HindIII A'AGCT_T
Cuts at: 0 1318 3862 4203
Size: 1318 2544 341
Fragments arranged by size:
2544 1318 341
KasI G'GCGC_C
Cuts at: 0 2852 4203
Size: 2852 1351
KpnI G_GTAC'C
Cuts at: 0 2005 3975 4203
Size: 2005 1970 228
Fragments arranged by size:
2005 1970 228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 1787 2993 4203
Size: 515 1272 1206 1210
Fragments arranged by size:
1272 1210 1206 515
MscI TGG'CCA
Cuts at: 0 403 1118 1285 3247 3445 3917 4203
Size: 403 715 167 1962 198 472 286
Fragments arranged by size:
1962 715 472 403 286 198 167
MslI CAynn'nnrTG
Cuts at: 0 341 736 977 1118 2386 3290 3565 4203
Size: 341 395 241 141 1268 904 275 638
Fragments arranged by size:
1268 904 638 395 341 275 241 141
MspA1I CmG'CkG
Cuts at: 0 1278 2262 2811 3106 3334 3636 3696 4203
Size: 1278 984 549 295 228 302 60 507
Fragments arranged by size:
1278 984 549 507 302 295 228 60
NaeI GCC'GGC
Cuts at: 0 2851 4203
Size: 2851 1352
NarI GG'CG_CC
Cuts at: 0 2853 4203
Size: 2853 1350
NcoI C'CATG_G
Cuts at: 0 398 471 1019 1113 2927 4203
Size: 398 73 548 94 1814 1276
Fragments arranged by size:
1814 1276 548 398 94 73
NdeI CA'TA_TG
Cuts at: 0 707 4203
Size: 707 3496
NgoMIV G'CCGG_C
Cuts at: 0 2849 4203
Size: 2849 1354
NspI r_CATG'y
Cuts at: 0 957 4203
Size: 957 3246
PasI CC'CwG_GG
Cuts at: 0 1491 4203
Size: 1491 2712
PflMI CCAn_nnn'nTGG
Cuts at: 0 1095 1212 1281 3961 3981 4203
Size: 1095 117 69 2680 20 222
Fragments arranged by size:
2680 1095 222 117 69 20
PfoI T'CCnGG_A
Cuts at: 0 296 380 1628 1891 2066 2864 3185 3302
Size: 296 84 1248 263 175 798 321 117
Cuts at: 3302 4203
Size: 901
Fragments arranged by size:
1248 901 798 321 296 263 175 117
84
PpuMI rG'GwC_Cy
Cuts at: 0 2727 3306 4203
Size: 2727 579 897
Fragments arranged by size:
2727 897 579
PshAI GACnn'nnGTC
Cuts at: 0 542 4203
Size: 542 3661
PsiI TTA'TAA
Cuts at: 0 701 4203
Size: 701 3502
PspOMI G'GGCC_C
Cuts at: 0 154 1382 2640 4203
Size: 154 1228 1258 1563
Fragments arranged by size:
1563 1258 1228 154
PstI C_TGCA'G
Cuts at: 0 173 746 1040 1352 1401 1413 1472 1874
Size: 173 573 294 312 49 12 59 402
Cuts at: 1874 2807 3105 4203
Size: 933 298 1098
Fragments arranged by size:
1098 933 573 402 312 298 294 173
59 49 12
PvuII CAG'CTG
Cuts at: 0 1278 2262 2811 3106 3696 4203
Size: 1278 984 549 295 590 507
Fragments arranged by size:
1278 984 590 549 507 295
SacI G_AGCT'C
Cuts at: 0 150 2846 2917 3467 4203
Size: 150 2696 71 550 736
Fragments arranged by size:
2696 736 550 150 71
SanDI GG'GwC_CC
Cuts at: 0 3306 4203
Size: 3306 897
SapI GCTCTTCn'nnn_
Cuts at: 0 808 1165 3542 4203
Size: 808 357 2377 661
Fragments arranged by size:
2377 808 661 357
SfcI C'TryA_G
Cuts at: 0 169 189 719 742 1036 1348 1397 1409
Size: 169 20 530 23 294 312 49 12
Cuts at: 1409 1468 1480 1754 1870 1881 1949 2424 2803
Size: 59 12 274 116 11 68 475 379
Cuts at: 2803 3081 3101 3989 4203
Size: 278 20 888 214
Fragments arranged by size:
888 530 475 379 312 294 278 274
214 169 116 68 59 49 23 20
20 12 12 11
SfoI GGC'GCC
Cuts at: 0 2854 4203
Size: 2854 1349
SmaI CCC'GGG
Cuts at: 0 1747 4203
Size: 1747 2456
SmlI C'TyrA_G
Cuts at: 0 1607 2203 3361 3907 4095 4203
Size: 1607 596 1158 546 188 108
Fragments arranged by size:
1607 1158 596 546 188 108
SpeI A'CTAG_T
Cuts at: 0 1839 4203
Size: 1839 2364
StuI AGG'CCT
Cuts at: 0 3077 3716 4203
Size: 3077 639 487
Fragments arranged by size:
3077 639 487
StyI C'CwwG_G
Cuts at: 0 398 432 461 471 1019 1113 2132 2897
Size: 398 34 29 10 548 94 1019 765
Cuts at: 2897 2927 3120 3593 4203
Size: 30 193 473 610
Fragments arranged by size:
1019 765 610 548 473 398 193 94
34 30 29 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2591 4069 4167 4203
Size: 2591 1478 98 36
Fragments arranged by size:
2591 1478 98 36
TatI w'GTAC_w
Cuts at: 0 768 4203
Size: 768 3435
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2576 2608 3613 3645 4203
Size: 2576 32 1005 32 558
Fragments arranged by size:
2576 1005 558 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4009 4203
Size: 4009 194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1026 1278 1430 2014 2585 3240 4203
Size: 1026 252 152 584 571 655 963
Fragments arranged by size:
1026 963 655 584 571 252 152
XhoI C'TCGA_G
Cuts at: 0 1607 4203
Size: 1607 2596
XmaI C'CCGG_G
Cuts at: 0 1745 4203
Size: 1745 2458
XmnI GAAnn'nnTTC
Cuts at: 0 2481 3454 4203
Size: 2481 973 749
Fragments arranged by size:
2481 973 749
ZraI GAC'GTC
Cuts at: 0 1234 4203
Size: 1234 2969
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApoI AseI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtsI
CspCI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HincII HindIII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PasI PflMI PfoI PpuMI PshAI
PsiI PspOMI PstI PvuII SacI SanDI SapI SfcI
SfoI SmaI SmlI SpeI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII AgeI ApaLI AscI AsiSI AvrII BaeI
BbvCI BfrBI BglI BmtI BplI BsaAI BsaBI BsiEI
BsiWI BspEI BsrGI BssHII BstEII BstZ17I BtgZI ClaI
DraI DraIII EagI EcoNI FseI FspI FspAI HpaI
MfeI MluI NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PspXI PsrI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SphI SrfI SspI SwaI XbaI