(Circular) (Six-base) MAPSORT of: pFN21AA0143.seq Check: 4983 from: 1 to: 7168 pFN21AA0143 7168 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4978 278 Size: 53 83 186 936 3442 2468 Fragments arranged by size: 3442 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4511 1937 Size: 2574 4594 Acc65I G'GTAC_C Cuts at: 4489 4489 Size: 7168 AclI AA'CG_TT Cuts at: 1563 3633 5295 5668 1563 Size: 2070 1662 373 3063 Fragments arranged by size: 3063 2070 1662 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2100 3910 4194 5231 6615 6883 1632 Size: 468 1810 284 1037 1384 268 1917 Fragments arranged by size: 1917 1810 1384 1037 468 284 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6971 AflIII A'CryG_T Cuts at: 1203 6067 7044 1203 Size: 4864 977 1327 Fragments arranged by size: 4864 1327 977 AgeI A'CCGG_T Cuts at: 5999 5999 Size: 7168 AhdI GACnn_n'nnGTC Cuts at: 3437 5897 3437 Size: 2460 4708 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7168 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2845 1184 Size: 1661 5507 AlwNI CAG_nnn'CTG Cuts at: 2141 2956 4911 6483 2141 Size: 815 1955 1572 2826 Fragments arranged by size: 2826 1955 1572 815 ApaLI G'TGCA_C Cuts at: 5225 6381 5225 Size: 1156 6012 ApoI r'AATT_y Cuts at: 1493 2365 2378 2816 3053 3297 3546 3882 4265 Size: 872 13 438 237 244 249 336 383 Cuts at: 4265 4318 4477 4703 4855 6964 1493 Size: 53 159 226 152 2109 1697 Fragments arranged by size: 2109 1697 872 438 383 336 249 244 237 226 159 152 53 13 AseI AT'TA_AT Cuts at: 160 1032 5722 160 Size: 872 4690 1606 Fragments arranged by size: 4690 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7168 AvaI C'yCGr_G Cuts at: 1870 1943 2013 2928 4493 1870 Size: 73 70 915 1565 4545 Fragments arranged by size: 4545 1565 915 73 70 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3578 3611 3578 Size: 33 7135 BamHI G'GATC_C Cuts at: 2806 3388 4498 4865 2806 Size: 582 1110 367 5109 Fragments arranged by size: 5109 1110 582 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4489 5944 618 Size: 325 217 627 2702 1455 1842 Fragments arranged by size: 2702 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4487 1391 Size: 3096 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2403 3007 3359 3633 928 Size: 971 504 604 352 274 4463 Fragments arranged by size: 4463 971 604 504 352 274 BbvCI CC'TCA_GC Cuts at: 2577 2577 Size: 7168 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2004 2021 2038 5359 5393 1903 Size: 34 50 17 17 17 3321 34 3678 Fragments arranged by size: 3678 3321 50 34 34 17 17 17 BciVI GTATCCnnnnn_n' Cuts at: 4123 5060 6270 4123 Size: 937 1210 5021 Fragments arranged by size: 5021 1210 937 BclI T'GATC_A Cuts at: 1548 1548 Size: 7168 BfrBI ATG'CAT Cuts at: 3454 3454 Size: 7168 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5779 136 Size: 107 122 71 631 605 4107 1525 Fragments arranged by size: 4107 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3317 4453 7163 3317 Size: 1136 2710 3322 Fragments arranged by size: 3322 2710 1136 BlpI GC'TnA_GC Cuts at: 4585 4585 Size: 7168 Bme1580I G_kGCm'C Cuts at: 1790 5229 6385 1790 Size: 3439 1156 2573 Fragments arranged by size: 3439 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7168 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4368 4424 4899 Size: 416 53 83 399 24 2951 56 475 Cuts at: 4899 5857 442 Size: 958 1753 Fragments arranged by size: 2951 1753 958 475 416 399 83 56 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7168 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2130 5828 1239 Size: 891 3698 2579 Fragments arranged by size: 3698 2579 891 Bpu10I CC'TnA_GC Cuts at: 2577 2577 Size: 7168 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5293 6158 6456 5293 Size: 865 298 6005 Fragments arranged by size: 6005 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5831 882 Size: 617 4332 2219 Fragments arranged by size: 4332 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4959 493 Size: 711 3755 2702 Fragments arranged by size: 3755 2702 711 BsaBI GATnn'nnATC Cuts at: 4074 7162 4074 Size: 3088 4080 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4249 4975 5357 5992 Size: 53 83 186 936 2716 726 382 635 Cuts at: 5992 275 Size: 1451 Fragments arranged by size: 2716 1451 936 726 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3511 5601 5999 6273 6420 6831 3511 Size: 2090 398 274 147 411 3848 Fragments arranged by size: 3848 2090 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6943 Fragments arranged by size: 6943 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4359 1186 Size: 3173 3995 BseYI C'CCAG_C Cuts at: 2728 6371 6867 2728 Size: 3643 496 3029 Fragments arranged by size: 3643 3029 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3158 3351 1163 Size: 573 1422 193 4980 Fragments arranged by size: 4980 1422 573 193 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4618 5379 6407 1540 Size: 275 186 2617 761 1028 2301 Fragments arranged by size: 2617 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5229 5314 6385 5229 Size: 85 1071 6012 Fragments arranged by size: 6012 1071 85 BsmI GAATG_Cn' Cuts at: 3148 4679 4772 3148 Size: 1531 93 5544 Fragments arranged by size: 5544 1531 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3047 3047 Size: 7168 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4487 5229 5314 6385 1391 Size: 399 2697 742 85 1071 2174 Fragments arranged by size: 2697 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6831 6831 Size: 7168 BspHI T'CATG_A Cuts at: 3207 3472 3570 4327 5057 6787 3207 Size: 265 98 757 730 1730 3588 Fragments arranged by size: 3588 1730 757 730 265 98 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2930 4523 844 Size: 2086 1593 3489 Fragments arranged by size: 3489 2086 1593 BsrBI CCG'CTC Cuts at: 1394 1823 5055 1394 Size: 429 3232 3507 Fragments arranged by size: 3507 3232 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2973 3729 5663 5837 65 Size: 1183 1725 756 1934 174 1396 Fragments arranged by size: 1934 1725 1396 1183 756 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5812 5999 1700 Size: 110 4002 187 2869 Fragments arranged by size: 4002 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7168 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7168 BssSI C'ACGA_G Cuts at: 5228 6240 5228 Size: 1012 6156 BstAPI GCAn_nnn'nTGC Cuts at: 794 2141 3806 794 Size: 1347 1665 4156 Fragments arranged by size: 4156 1665 1347 BstBI TT'CG_AA Cuts at: 3057 3886 5972 3057 Size: 829 2086 4253 Fragments arranged by size: 4253 2086 829 BstEII G'GTnAC_C Cuts at: 1184 3331 1184 Size: 2147 5021 BstXI CCAn_nnnn'nTGG Cuts at: 3788 4123 4222 3788 Size: 335 99 6734 Fragments arranged by size: 6734 335 99 BstYI r'GATC_y Cuts at: 1972 2083 2806 3080 3317 3388 4453 4498 4865 Size: 111 723 274 237 71 1065 45 367 Cuts at: 4865 5250 5267 6719 6805 6817 7163 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1065 723 385 367 346 274 237 111 86 71 45 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6616 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6429 Fragments arranged by size: 6429 683 56 BtsI GCAGTG_nn' Cuts at: 2827 3097 3368 4684 5478 5498 2827 Size: 270 271 1316 794 20 4497 Fragments arranged by size: 4497 1316 794 271 270 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7168 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7133 DraI TTT'AAA Cuts at: 2836 4472 2836 Size: 1636 5532 DraIII CAC_nnn'GTG Cuts at: 2878 4304 2878 Size: 1426 5742 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6175 809 Size: 507 4859 1802 Fragments arranged by size: 4859 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3726 4615 5504 Size: 54 1425 50 275 18 1896 889 889 Cuts at: 5504 7133 8 Size: 1629 43 Fragments arranged by size: 1896 1629 1425 889 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4615 1537 Size: 275 2803 4090 Fragments arranged by size: 4090 2803 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2679 5098 1353 Size: 1326 2419 3423 Fragments arranged by size: 3423 2419 1326 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5755 6129 6275 387 Size: 1064 138 4166 374 146 1280 Fragments arranged by size: 4166 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2100 2130 3910 4194 5231 5828 6615 Size: 393 468 30 1780 284 1037 597 787 Cuts at: 6615 6883 1239 Size: 268 1524 Fragments arranged by size: 1780 1524 1037 787 597 468 393 284 268 30 EcoO109I rG'GnC_Cy Cuts at: 2651 2651 Size: 7168 EcoRI G'AATT_C Cuts at: 3053 3297 4477 4855 3053 Size: 244 1180 378 5366 Fragments arranged by size: 5366 1180 378 244 EcoRV GAT'ATC Cuts at: 3265 3265 Size: 7168 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2114 4097 2114 Size: 1983 5185 FspI TGC'GCA Cuts at: 2781 5674 2781 Size: 2893 4275 HaeII r_GCGC'y Cuts at: 1678 6315 1678 Size: 4637 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2390 2422 3423 3455 4025 Size: 32 135 32 818 32 1001 32 570 Cuts at: 4025 4057 4097 4118 4129 4150 5809 5841 5883 Size: 32 40 21 11 21 1659 32 42 Cuts at: 5883 5915 1373 Size: 32 2626 Fragments arranged by size: 2626 1659 1001 818 570 135 42 40 32 32 32 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 669 1657 1938 2545 2638 3097 3272 4512 4758 Size: 988 281 607 93 459 175 1240 246 Cuts at: 4758 669 Size: 3079 Fragments arranged by size: 3079 1240 988 607 459 281 246 175 93 HindIII A'AGCT_T Cuts at: 748 2299 2345 3488 748 Size: 1551 46 1143 4428 Fragments arranged by size: 4428 1551 1143 46 HpaI GTT'AAC Cuts at: 4758 4758 Size: 7168 KpnI G_GTAC'C Cuts at: 4493 4493 Size: 7168 MfeI C'AATT_G Cuts at: 4206 4767 4206 Size: 561 6607 MluI A'CGCG_T Cuts at: 7044 7044 Size: 7168 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3023 4161 6282 6466 1305 Size: 1718 1138 2121 184 2007 Fragments arranged by size: 2121 2007 1718 1138 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3728 10 Size: 54 1425 343 1896 3450 Fragments arranged by size: 3450 1896 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2904 3786 4121 5126 5485 5644 518 Size: 648 1738 882 335 1005 359 159 2042 Fragments arranged by size: 2042 1738 1005 882 648 359 335 159 MspA1I CmG'CkG Cuts at: 1606 3592 4584 5261 6409 6654 1606 Size: 1986 992 677 1148 245 2120 Fragments arranged by size: 2120 1986 1148 992 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6616 NdeI CA'TA_TG Cuts at: 387 2310 2583 387 Size: 1923 273 4972 Fragments arranged by size: 4972 1923 273 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7168 NotI GC'GGCC_GC Cuts at: 4615 4615 Size: 7168 NsiI A_TGCA'T Cuts at: 3456 3456 Size: 7168 NspI r_CATG'y Cuts at: 1132 1229 2620 3866 3943 4412 4526 6071 1132 Size: 97 1391 1246 77 469 114 1545 2229 Fragments arranged by size: 2229 1545 1391 1246 469 114 97 77 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7168 PciI A'CATG_T Cuts at: 6067 6067 Size: 7168 PflMI CCAn_nnn'nTGG Cuts at: 1108 2093 2568 3259 4348 1108 Size: 985 475 691 1089 3928 Fragments arranged by size: 3928 1089 985 691 475 PfoI T'CCnGG_A Cuts at: 2883 3384 2883 Size: 501 6667 PmeI GTTT'AAAC Cuts at: 4472 4472 Size: 7168 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5237 5269 6750 6782 5237 Size: 32 1481 32 5623 Fragments arranged by size: 5623 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7168 PsiI TTA'TAA Cuts at: 2843 3677 4738 2843 Size: 834 1061 5273 Fragments arranged by size: 5273 1061 834 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4344 4376 4344 Size: 32 7136 PstI C_TGCA'G Cuts at: 830 4520 830 Size: 3690 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7168 PvuII CAG'CTG Cuts at: 3592 3592 Size: 7168 SalI G'TCGA_C Cuts at: 1936 4510 1936 Size: 2574 4594 SbfI CC_TGCA'GG Cuts at: 4520 4520 Size: 7168 ScaI AGT'ACT Cuts at: 5416 7128 5416 Size: 1712 5456 SfcI C'TryA_G Cuts at: 826 1045 3084 4516 5651 6332 6523 826 Size: 219 2039 1432 1135 681 191 1471 Fragments arranged by size: 2039 1471 1432 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7168 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7168 SmaI CCC'GGG Cuts at: 1872 2930 4495 1872 Size: 1058 1565 4545 Fragments arranged by size: 4545 1565 1058 SmlI C'TyrA_G Cuts at: 820 1017 1943 5272 6173 6435 820 Size: 197 926 3329 901 262 1553 Fragments arranged by size: 3329 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4959 493 Size: 4466 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7168 SphI G_CATG'C Cuts at: 1132 3943 4412 4526 1132 Size: 2811 469 114 3774 Fragments arranged by size: 3774 2811 469 114 SspI AAT'ATT Cuts at: 5 52 2389 4347 5092 5 Size: 47 2337 1958 745 2081 Fragments arranged by size: 2337 2081 1958 745 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7168 StyI C'CwwG_G Cuts at: 513 1065 4607 513 Size: 552 3542 3074 Fragments arranged by size: 3542 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5195 5212 5365 5550 5995 325 Size: 1255 3615 17 153 185 445 1498 Fragments arranged by size: 3615 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3978 5414 7126 Size: 274 80 33 51 1443 2001 1436 1712 Cuts at: 7126 96 Size: 138 Fragments arranged by size: 2001 1712 1443 1436 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4348 4380 7128 7160 4348 Size: 32 2748 32 4356 Fragments arranged by size: 4356 2748 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7168 XbaI T'CTAG_A Cuts at: 4085 4504 4085 Size: 419 6749 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7168 XmaI C'CCGG_G Cuts at: 1870 2928 4493 1870 Size: 1058 1565 4545 Fragments arranged by size: 4545 1565 1058 XmnI GAAnn'nnTTC Cuts at: 2167 2301 2365 5297 2167 Size: 134 64 2932 4038 Fragments arranged by size: 4038 2932 134 64 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4976 276 Size: 53 83 186 936 3442 2468 Fragments arranged by size: 3442 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI