Restriction map for
pFN21AA0131
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# NO CUT ENZYMES ( 82 enzymes) #
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Acc65I AclI AflII AhdI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BciVI
BclI BfrI BlnI BsaBI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII DrdI
Eam1105I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HpaI KpnI
KspI MamI MfeI MluI MluNI
MscI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PmeI PshBI Psp1406I RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 790 GACGTC GACGT^C
AccI 2113 GTCTAC GT^[AC][GT]AC
AcsI 1459 AAATTC [GA]^AATT[CT]
AflIII 2589 ACACGT A^C[GA][CT]GT
AgeI 1995 ACCGGT A^CCGGT
Alw44I 714 GTGCAC G^TGCAC
AocI 2527 CCTGAGG CC^T[ACGT]AGG
Aor51HI 215 AGCGCT AGC^GCT
ApaLI 714 GTGCAC G^TGCAC
ApoI 1459 AAATTC [GA]^AATT[CT]
BamHI 936 GGATCC G^GATCC
BglII 1040 AGATCT A^GATCT
BsmBI 52 GAGACG CGTCTC(1/5)
BsrDI 402 CATTGC GCAATG(2/0)
BssHII 724 GCGCGC G^CGCGC
Bsu36I 2527 CCTGAGG CC^T[ACGT]AGG
EagI 863 CGGCCG C^GGCCG
EclXI 863 CGGCCG C^GGCCG
Eco47III 215 AGCGCT AGC^GCT
Eco52I 863 CGGCCG C^GGCCG
Eco81I 2527 CCTGAGG CC^T[ACGT]AGG
HincII 757 GTCAAC GT[CT]^[GA]AC
HindII 757 GTCAAC GT[CT]^[GA]AC
HindIII 264 AAGCTT A^AGCTT
MseI 2636 TTAA T^TAA
MslI 1012 CACCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1268 GCCGGC GCC^GGC
NcoI 2534 CCATGG C^CATGG
NgoMIV 1268 GCCGGC G^CCGGC
PflMI 1713 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1995 ACCGGT A^CCGGT
PvuI 2073 CGATCG CGAT^CG
SgrAI 1877 CGCCGGTG C[GA]^CCGG[CT]G
Tru9I 2636 TTAA T^TAA
Tsp509I 1460 AATT ^AATT
Van91I 1713 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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BbeI 161 GGCGCC GGCGC^C
419 GGCGCC
BbrPI 1846 CACGTG CAC^GTG
2590 CACGTG
BbvCI 2792 CCTCAGC CCTCAGC(-5/-2)
169 GCTGAGG
BcgI 966 CGAGGTGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2067 GCAGCCCGATCG
BfaI 752 CTAG C^TAG
2815 CTAG
BsaAI 1846 CACGTG [CT]AC^GT[GA]
2590 CACGTG
BsiEI 863 CGGCCG CG[GA][CT]^CG
2073 CGATCG
BsmI 537 GAATGC GAATGC(1/-1)
1580 GCATTC
BssSI 1381 CACGAG CACGAG(-5/-1)
2326 CACGAG
BstAPI 450 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1161 GCAGGCCCTGC
DsaI 228 CCGTGG C^C[GA][CT]GG
2534 CCATGG
KasI 161 GGCGCC G^GCGCC
419 GGCGCC
MaeI 752 CTAG C^TAG
2815 CTAG
NarI 161 GGCGCC GG^CGCC
419 GGCGCC
NspI 972 GCATGC [GA]CATG^[CT]
2369 GCATGC
PaeR7I 964 CTCGAG C^TCGAG
2337 CTCGAG
PmaCI 1846 CACGTG CAC^GTG
2590 CACGTG
PmlI 1846 CACGTG CAC^GTG
2590 CACGTG
PshAI 54 GACGCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2655 GACCTCTGTC
SapI 432 GAAGAGC GCTCTTC(1/4)
1467 GAAGAGC
SfoI 161 GGCGCC GGC^GCC
419 GGCGCC
SmaI 191 CCCGGG CCC^GGG
2765 CCCGGG
SphI 972 GCATGC GCATG^C
2369 GCATGC
TfiI 1071 GATTC G^A[AT]TC
1207 GATTC
XhoI 964 CTCGAG C^TCGAG
2337 CTCGAG
XmaCI 191 CCCGGG C^CCGGG
2765 CCCGGG
XmaI 191 CCCGGG C^CCGGG
2765 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MroI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XcmI XhoII