Restriction map for
pFN21AA0131
(Linear) (Six-base) MAPSORT of: KIAA0131.seq Check: 1065 from: 1 to: 2838
KIAA0131.orf
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 834 2415 2838
Size: 834 1581 423
Fragments arranged by size:
1581 834 423
AatII G_ACGT'C
Cuts at: 0 794 2838
Size: 794 2044
AccI GT'mk_AC
Cuts at: 0 2114 2838
Size: 2114 724
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1168 1387 1762 2838
Size: 1168 219 375 1076
Fragments arranged by size:
1168 1076 375 219
AfeI AGC'GCT
Cuts at: 0 217 2838
Size: 217 2621
AflIII A'CryG_T
Cuts at: 0 2589 2838
Size: 2589 249
AgeI A'CCGG_T
Cuts at: 0 1995 2838
Size: 1995 843
AlwNI CAG_nnn'CTG
Cuts at: 0 144 393 456 903 1167 2292 2838
Size: 144 249 63 447 264 1125 546
Fragments arranged by size:
1125 546 447 264 249 144 63
ApaI G_GGCC'C
Cuts at: 0 380 635 1985 2711 2773 2781 2838
Size: 380 255 1350 726 62 8 57
Fragments arranged by size:
1350 726 380 255 62 57 8
ApaLI G'TGCA_C
Cuts at: 0 714 2838
Size: 714 2124
ApoI r'AATT_y
Cuts at: 0 1459 2838
Size: 1459 1379
AvaI C'yCGr_G
Cuts at: 0 191 964 2337 2473 2765 2838
Size: 191 773 1373 136 292 73
Fragments arranged by size:
1373 773 292 191 136 73
BamHI G'GATC_C
Cuts at: 0 936 2838
Size: 936 1902
BanI G'GyrC_C
Cuts at: 0 161 419 616 1636 1998 2130 2838
Size: 161 258 197 1020 362 132 708
Fragments arranged by size:
1020 708 362 258 197 161 132
BanII G_rGCy'C
Cuts at: 0 380 470 491 635 649 1086 1546 1769
Size: 380 90 21 144 14 437 460 223
Cuts at: 1769 1985 2645 2711 2773 2781 2838
Size: 216 660 66 62 8 57
Fragments arranged by size:
660 460 437 380 223 216 144 90
66 62 57 21 14 8
BbeI G_GCGC'C
Cuts at: 0 165 423 2838
Size: 165 258 2415
Fragments arranged by size:
2415 258 165
BbsI GAAGACnn'nnnn_
Cuts at: 0 511 975 2073 2838
Size: 511 464 1098 765
Fragments arranged by size:
1098 765 511 464
BbvCI CC'TCA_GC
Cuts at: 0 170 2793 2838
Size: 170 2623 45
Fragments arranged by size:
2623 170 45
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 955 989 2056 2090 2838
Size: 955 34 1067 34 748
Fragments arranged by size:
1067 955 748 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 233 625 2007 2744 2838
Size: 233 392 1382 737 94
Fragments arranged by size:
1382 737 392 233 94
BglII A'GATC_T
Cuts at: 0 1040 2838
Size: 1040 1798
BlpI GC'TnA_GC
Cuts at: 0 82 742 2293 2838
Size: 82 660 1551 545
Fragments arranged by size:
1551 660 545 82
Bme1580I G_kGCm'C
Cuts at: 0 380 635 718 1562 1641 1985 2003 2711
Size: 380 255 83 844 79 344 18 708
Cuts at: 2711 2773 2781 2838
Size: 62 8 57
Fragments arranged by size:
844 708 380 344 255 83 79 62
57 18 8
BmgBI CAC'GTC
Cuts at: 0 414 2838
Size: 414 2424
BmrI ACTGGGnnnn_n'
Cuts at: 0 1437 1488 1923 2693 2838
Size: 1437 51 435 770 145
Fragments arranged by size:
1437 770 435 145 51
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 237 498 2352 2838
Size: 237 261 1854 486
Fragments arranged by size:
1854 486 261 237
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 207 466 475 493 926 1192 1315
Size: 124 83 259 9 18 433 266 123
Cuts at: 1315 1783 1840 2188 2254 2838
Size: 468 57 348 66 584
Fragments arranged by size:
584 468 433 348 266 259 124 123
83 66 57 18 9
Bpu10I CC'TnA_GC
Cuts at: 0 170 1545 2793 2838
Size: 170 1375 1248 45
Fragments arranged by size:
1375 1248 170 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 694 1225 2838
Size: 637 57 531 1613
Fragments arranged by size:
1613 637 531 57
BsaI GGTCTCn'nnnn_
Cuts at: 0 486 1297 1656 2838
Size: 486 811 359 1182
Fragments arranged by size:
1182 811 486 359
BsaAI yAC'GTr
Cuts at: 0 1848 2592 2838
Size: 1848 744 246
Fragments arranged by size:
1848 744 246
BsaHI Gr'CG_yC
Cuts at: 0 162 420 791 2159 2838
Size: 162 258 371 1368 679
Fragments arranged by size:
1368 679 371 258 162
BsaWI w'CCGG_w
Cuts at: 0 269 851 1754 1995 2838
Size: 269 582 903 241 843
Fragments arranged by size:
903 843 582 269 241
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2679 2709 2838
Size: 2679 30 129
Fragments arranged by size:
2679 129 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 589 1144 1435 2838
Size: 589 555 291 1403
Fragments arranged by size:
1403 589 555 291
BseYI C'CCAG_C
Cuts at: 0 305 386 1486 1640 2005 2162 2571 2731
Size: 305 81 1100 154 365 157 409 160
Cuts at: 2731 2781 2838
Size: 50 57
Fragments arranged by size:
1100 409 365 305 160 157 154 81
57 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 1689 2071 2086 2308 2838
Size: 916 773 382 15 222 530
Fragments arranged by size:
916 773 530 382 222 15
BsiEI CG_ry'CG
Cuts at: 0 866 2076 2838
Size: 866 1210 762
Fragments arranged by size:
1210 866 762
BsiHKAI G_wGCw'C
Cuts at: 0 260 470 491 649 704 718 851 1546
Size: 260 210 21 158 55 14 133 695
Cuts at: 1546 1847 2363 2645 2838
Size: 301 516 282 193
Fragments arranged by size:
695 516 301 282 260 210 193 158
133 55 21 14
BsmI GAATG_Cn'
Cuts at: 0 543 1580 2838
Size: 543 1037 1258
Fragments arranged by size:
1258 1037 543
BsmBI CGTCTCn'nnnn_
Cuts at: 0 46 2838
Size: 46 2792
Bsp1286I G_dGCh'C
Cuts at: 0 260 380 470 491 635 649 704 718
Size: 260 120 90 21 144 14 55 14
Cuts at: 718 851 1086 1546 1562 1641 1769 1847 1985
Size: 133 235 460 16 79 128 78 138
Cuts at: 1985 2003 2363 2645 2711 2773 2781 2838
Size: 18 360 282 66 62 8 57
Fragments arranged by size:
460 360 282 260 235 144 138 133
128 120 90 79 78 66 62 57
55 21 18 16 14 14 8
BspEI T'CCGG_A
Cuts at: 0 269 851 1754 2838
Size: 269 582 903 1084
Fragments arranged by size:
1084 903 582 269
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 746 791 834 2415 2838
Size: 746 45 43 1581 423
Fragments arranged by size:
1581 746 423 45 43
BsrBI CCG'CTC
Cuts at: 0 7 30 567 2838
Size: 7 23 537 2271
Fragments arranged by size:
2271 537 23 7
BsrDI GCAATG_nn'
Cuts at: 0 401 2838
Size: 401 2437
BsrFI r'CCGG_y
Cuts at: 0 1268 1878 1995 2504 2838
Size: 1268 610 117 509 334
Fragments arranged by size:
1268 610 509 334 117
BssHII G'CGCG_C
Cuts at: 0 724 2838
Size: 724 2114
BssSI C'ACGA_G
Cuts at: 0 1381 2326 2838
Size: 1381 945 512
Fragments arranged by size:
1381 945 512
BstAPI GCAn_nnn'nTGC
Cuts at: 0 456 1167 2838
Size: 456 711 1671
Fragments arranged by size:
1671 711 456
BstXI CCAn_nnnn'nTGG
Cuts at: 0 696 2151 2674 2838
Size: 696 1455 523 164
Fragments arranged by size:
1455 696 523 164
BstYI r'GATC_y
Cuts at: 0 441 936 1040 1658 2838
Size: 441 495 104 618 1180
Fragments arranged by size:
1180 618 495 441 104
Bsu36I CC'TnA_GG
Cuts at: 0 2528 2838
Size: 2528 310
BtgI C'CryG_G
Cuts at: 0 228 2534 2838
Size: 228 2306 304
Fragments arranged by size:
2306 304 228
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1952 2838
Size: 1952 886
BtsI GCAGTG_nn'
Cuts at: 0 300 1156 1706 2838
Size: 300 856 550 1132
Fragments arranged by size:
1132 856 550 300
EaeI y'GGCC_r
Cuts at: 0 2 209 371 497 863 1290 1730 1967
Size: 2 207 162 126 366 427 440 237
Cuts at: 1967 2506 2838
Size: 539 332
Fragments arranged by size:
539 440 427 366 332 237 207 162
126 2
EagI C'GGCC_G
Cuts at: 0 863 2838
Size: 863 1975
EarI CTCTTCn'nnn_
Cuts at: 0 427 1462 1780 1909 2838
Size: 427 1035 318 129 929
Fragments arranged by size:
1035 929 427 318 129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2130 2197 2653 2838
Size: 2130 67 456 185
Fragments arranged by size:
2130 456 185 67
EcoICRI GAG'CTC
Cuts at: 0 468 489 647 1544 2643 2838
Size: 468 21 158 897 1099 195
Fragments arranged by size:
1099 897 468 195 158 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 207 466 475 493 926 1168 1192
Size: 124 83 259 9 18 433 242 24
Cuts at: 1192 1315 1387 1762 1783 1840 2188 2254 2838
Size: 123 72 375 21 57 348 66 584
Fragments arranged by size:
584 433 375 348 259 242 124 123
83 72 66 57 24 21 18 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 361 655 841 1171 2838
Size: 361 294 186 330 1667
Fragments arranged by size:
1667 361 330 294 186
EcoO109I rG'GnC_Cy
Cuts at: 0 195 377 631 632 900 930 1164 1398
Size: 195 182 254 1 268 30 234 234
Cuts at: 1398 1686 2013 2333 2376 2478 2539 2670 2708
Size: 288 327 320 43 102 61 131 38
Cuts at: 2708 2724 2769 2770 2777 2778 2811 2838
Size: 16 45 1 7 1 33 27
Fragments arranged by size:
327 320 288 268 254 234 234 195
182 131 102 61 45 43 38 33
30 27 16 7 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1767 2661 2838
Size: 1767 894 177
Fragments arranged by size:
1767 894 177
HaeII r_GCGC'y
Cuts at: 0 165 219 393 423 2026 2838
Size: 165 54 174 30 1603 812
Fragments arranged by size:
1603 812 174 165 54 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 426 458 1340 1372 1708 1740 2838
Size: 426 32 882 32 336 32 1098
Fragments arranged by size:
1098 882 426 336 32 32 32
HincII GTy'rAC
Cuts at: 0 759 2838
Size: 759 2079
HindIII A'AGCT_T
Cuts at: 0 264 2838
Size: 264 2574
KasI G'GCGC_C
Cuts at: 0 161 419 2838
Size: 161 258 2419
Fragments arranged by size:
2419 258 161
MslI CAynn'nnrTG
Cuts at: 0 1016 2838
Size: 1016 1822
MspA1I CmG'CkG
Cuts at: 0 81 93 456 613 868 996 1831 2005
Size: 81 12 363 157 255 128 835 174
Cuts at: 2005 2046 2147 2166 2838
Size: 41 101 19 672
Fragments arranged by size:
835 672 363 255 174 157 128 101
81 41 19 12
NaeI GCC'GGC
Cuts at: 0 1270 2838
Size: 1270 1568
NarI GG'CG_CC
Cuts at: 0 162 420 2838
Size: 162 258 2418
Fragments arranged by size:
2418 258 162
NcoI C'CATG_G
Cuts at: 0 2534 2838
Size: 2534 304
NgoMIV G'CCGG_C
Cuts at: 0 1268 2838
Size: 1268 1570
NspI r_CATG'y
Cuts at: 0 976 2373 2838
Size: 976 1397 465
Fragments arranged by size:
1397 976 465
PasI CC'CwG_GG
Cuts at: 0 837 2773 2808 2838
Size: 837 1936 35 30
Fragments arranged by size:
1936 837 35 30
PflMI CCAn_nnn'nTGG
Cuts at: 0 1719 2838
Size: 1719 1119
PfoI T'CCnGG_A
Cuts at: 0 470 554 1331 2838
Size: 470 84 777 1507
Fragments arranged by size:
1507 777 470 84
PmlI CAC'GTG
Cuts at: 0 1848 2592 2838
Size: 1848 744 246
Fragments arranged by size:
1848 744 246
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2611 2643 2838
Size: 2611 32 195
Fragments arranged by size:
2611 195 32
PpuMI rG'GwC_Cy
Cuts at: 0 1686 2013 2539 2838
Size: 1686 327 526 299
Fragments arranged by size:
1686 526 327 299
PshAI GACnn'nnGTC
Cuts at: 0 58 2659 2838
Size: 58 2601 179
Fragments arranged by size:
2601 179 58
PspOMI G'GGCC_C
Cuts at: 0 376 631 1981 2707 2769 2777 2838
Size: 376 255 1350 726 62 8 61
Fragments arranged by size:
1350 726 376 255 62 61 8
PspXI vC'TCGA_Gb
Cuts at: 0 2337 2838
Size: 2337 501
PstI C_TGCA'G
Cuts at: 0 41 113 140 323 461 1163 1373 1394
Size: 41 72 27 183 138 702 210 21
Cuts at: 1394 1610 2030 2447 2454 2838
Size: 216 420 417 7 384
Fragments arranged by size:
702 420 417 384 216 210 183 138
72 41 27 21 7
PvuI CG_AT'CG
Cuts at: 0 2076 2838
Size: 2076 762
PvuII CAG'CTG
Cuts at: 0 81 93 456 2046 2147 2166 2838
Size: 81 12 363 1590 101 19 672
Fragments arranged by size:
1590 672 363 101 81 19 12
SacI G_AGCT'C
Cuts at: 0 470 491 649 1546 2645 2838
Size: 470 21 158 897 1099 193
Fragments arranged by size:
1099 897 470 193 158 21
SanDI GG'GwC_CC
Cuts at: 0 2539 2838
Size: 2539 299
SapI GCTCTTCn'nnn_
Cuts at: 0 427 1462 2838
Size: 427 1035 1376
Fragments arranged by size:
1376 1035 427
SfcI C'TryA_G
Cuts at: 0 37 109 136 319 457 1159 1369 1390
Size: 37 72 27 183 138 702 210 21
Cuts at: 1390 1606 2026 2443 2450 2838
Size: 216 420 417 7 388
Fragments arranged by size:
702 420 417 388 216 210 183 138
72 37 27 21 7
SfoI GGC'GCC
Cuts at: 0 163 421 2838
Size: 163 258 2417
Fragments arranged by size:
2417 258 163
SgrAI Cr'CCGG_yG
Cuts at: 0 1878 2838
Size: 1878 960
SmaI CCC'GGG
Cuts at: 0 193 2767 2838
Size: 193 2574 71
Fragments arranged by size:
2574 193 71
SmlI C'TyrA_G
Cuts at: 0 652 709 964 1240 2337 2838
Size: 652 57 255 276 1097 501
Fragments arranged by size:
1097 652 501 276 255 57
SphI G_CATG'C
Cuts at: 0 976 2373 2838
Size: 976 1397 465
Fragments arranged by size:
1397 976 465
StyI C'CwwG_G
Cuts at: 0 956 2534 2673 2838
Size: 956 1578 139 165
Fragments arranged by size:
1578 956 165 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 895 1124 1321 2838
Size: 895 229 197 1517
Fragments arranged by size:
1517 895 229 197
TatI w'GTAC_w
Cuts at: 0 1445 1931 2838
Size: 1445 486 907
Fragments arranged by size:
1445 907 486
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1018 1510 2739 2838
Size: 1018 492 1229 99
Fragments arranged by size:
1229 1018 492 99
XhoI C'TCGA_G
Cuts at: 0 964 2337 2838
Size: 964 1373 501
Fragments arranged by size:
1373 964 501
XmaI C'CCGG_G
Cuts at: 0 191 2765 2838
Size: 191 2574 73
Fragments arranged by size:
2574 191 73
ZraI GAC'GTC
Cuts at: 0 792 2838
Size: 792 2046
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII HindIII KasI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI
PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI
PvuII SacI SanDI SapI SfcI SfoI SgrAI SmaI
SmlI SphI StyI TaqII TatI XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
Acc65I AclI AflII AhdI AleI AlfI AloI AscI
AseI AsiSI AvrII BaeI BciVI BclI BfrBI BmtI
BsaBI BsiWI BspHI BsrGI BstBI BstEII BstZ17I ClaI
CspCI DraI DraIII DrdI EcoRI EcoRV FseI FspI
FspAI HpaI KpnI MfeI MluI MmeI MscI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PsiI
PsrI RsrII SacII SalI SbfI ScaI SexAI SfiI
SgfI SnaBI SpeI SrfI SspI StuI SwaI TstI
Tth111I XbaI XmnI