(Circular) (Six-base) MAPSORT of: pFN21AA0113.seq Check: 4874 from: 1 to: 6613 pFN21AA0113 6613 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2493 3037 3388 2493 Size: 544 351 5718 Fragments arranged by size: 5718 544 351 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4423 278 Size: 53 83 186 936 2887 2468 Fragments arranged by size: 2887 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3802 3956 1937 Size: 1865 154 4594 Fragments arranged by size: 4594 1865 154 Acc65I G'GTAC_C Cuts at: 3934 3934 Size: 6613 AclI AA'CG_TT Cuts at: 1563 4740 5113 1563 Size: 3177 373 3063 Fragments arranged by size: 3177 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2129 2690 3494 4676 6060 6328 1632 Size: 497 561 804 1182 1384 268 1917 Fragments arranged by size: 1917 1384 1182 804 561 497 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6416 AflIII A'CryG_T Cuts at: 1203 5512 6489 1203 Size: 4309 977 1327 Fragments arranged by size: 4309 1327 977 AgeI A'CCGG_T Cuts at: 5444 5444 Size: 6613 AhdI GACnn_n'nnGTC Cuts at: 5342 5342 Size: 6613 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6613 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2492 2957 1184 Size: 1308 465 4840 Fragments arranged by size: 4840 1308 465 AlwNI CAG_nnn'CTG Cuts at: 2312 2494 2635 2837 3028 3367 4356 5928 2312 Size: 182 141 202 191 339 989 1572 2997 Fragments arranged by size: 2997 1572 989 339 202 191 182 141 ApaI G_GGCC'C Cuts at: 2450 3349 2450 Size: 899 5714 ApaLI G'TGCA_C Cuts at: 2597 4670 5826 2597 Size: 2073 1156 3384 Fragments arranged by size: 3384 2073 1156 ApoI r'AATT_y Cuts at: 1493 2098 2375 2404 2734 3922 4148 4300 6409 Size: 605 277 29 330 1188 226 152 2109 Cuts at: 6409 1493 Size: 1697 Fragments arranged by size: 2109 1697 1188 605 330 277 226 152 29 AseI AT'TA_AT Cuts at: 160 1032 5167 160 Size: 872 4135 1606 Fragments arranged by size: 4135 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6613 AvaI C'yCGr_G Cuts at: 1870 1943 3938 1870 Size: 73 1995 4545 Fragments arranged by size: 4545 1995 73 BamHI G'GATC_C Cuts at: 3943 4310 3943 Size: 367 6246 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2363 3679 3934 5389 618 Size: 325 217 627 576 1316 255 1455 1842 Fragments arranged by size: 1842 1455 1316 627 576 325 255 217 BanII G_rGCy'C Cuts at: 1391 2249 2271 2450 2745 3215 3332 3349 3621 Size: 858 22 179 295 470 117 17 272 Cuts at: 3621 3839 3932 1391 Size: 218 93 4072 Fragments arranged by size: 4072 858 470 295 272 218 179 117 93 22 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5642 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4804 4838 1903 Size: 34 2867 34 3678 Fragments arranged by size: 3678 2867 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4505 5715 4505 Size: 1210 5403 BclI T'GATC_A Cuts at: 1548 1548 Size: 6613 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5224 136 Size: 107 122 71 631 605 3552 1525 Fragments arranged by size: 3552 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3405 6608 3405 Size: 3203 3410 BlpI GC'TnA_GC Cuts at: 2265 3209 4030 2265 Size: 944 821 4848 Fragments arranged by size: 4848 944 821 Bme1580I G_kGCm'C Cuts at: 1790 2055 2450 2601 3349 4674 5830 1790 Size: 265 395 151 748 1325 1156 2573 Fragments arranged by size: 2573 1325 1156 748 395 265 151 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6613 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2316 4344 5302 Size: 416 53 83 399 24 899 2028 958 Cuts at: 5302 442 Size: 1753 Fragments arranged by size: 2028 1753 958 899 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6613 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3581 3581 Size: 6613 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2516 2591 2942 3080 3293 5273 1239 Size: 1277 75 351 138 213 1980 2579 Fragments arranged by size: 2579 1980 1277 351 213 138 75 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2729 4738 5603 5901 2729 Size: 2009 865 298 3441 Fragments arranged by size: 3441 2009 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2492 3131 5276 882 Size: 617 993 639 2145 2219 Fragments arranged by size: 2219 2145 993 639 617 BsaAI yAC'GTr Cuts at: 493 1204 4404 493 Size: 711 3200 2702 Fragments arranged by size: 3200 2702 711 BsaBI GATnn'nnATC Cuts at: 6607 6607 Size: 6613 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3799 4420 4802 5437 Size: 53 83 186 936 2266 621 382 635 Cuts at: 5437 275 Size: 1451 Fragments arranged by size: 2266 1451 936 635 621 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2025 2976 5046 5444 5718 5865 6276 2025 Size: 951 2070 398 274 147 411 2362 Fragments arranged by size: 2362 2070 951 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2284 2314 1178 Size: 30 165 30 881 30 5477 Fragments arranged by size: 5477 881 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2210 2359 2411 2537 3095 3149 3428 3473 Size: 1024 149 52 126 558 54 279 45 Cuts at: 3473 3560 1186 Size: 87 4239 Fragments arranged by size: 4239 1024 558 279 149 126 87 54 52 45 BseYI C'CCAG_C Cuts at: 2443 3522 5816 6312 2443 Size: 1079 2294 496 2744 Fragments arranged by size: 2744 2294 1079 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2465 1163 Size: 573 729 5311 Fragments arranged by size: 5311 729 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4063 4824 5852 1540 Size: 275 186 2062 761 1028 2301 Fragments arranged by size: 2301 2062 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2271 2601 2745 3747 3839 4674 4759 5830 2271 Size: 330 144 1002 92 835 85 1071 3054 Fragments arranged by size: 3054 1071 1002 835 330 144 92 85 BsmI GAATG_Cn' Cuts at: 3533 4124 4217 3533 Size: 591 93 5929 Fragments arranged by size: 5929 591 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2249 2271 2450 2601 2745 3215 Size: 399 265 194 22 179 151 144 470 Cuts at: 3215 3332 3349 3621 3747 3839 3932 4674 4759 Size: 117 17 272 126 92 93 742 85 Cuts at: 4759 5830 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 470 399 272 265 194 179 151 144 126 117 93 92 85 22 17 BspEI T'CCGG_A Cuts at: 6276 6276 Size: 6613 BspHI T'CATG_A Cuts at: 4502 6232 4502 Size: 1730 4883 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2493 2855 3037 3204 3388 3968 844 Size: 1649 362 182 167 184 580 3489 Fragments arranged by size: 3489 1649 580 362 184 182 167 BsrBI CCG'CTC Cuts at: 1394 1823 3070 4500 1394 Size: 429 1247 1430 3507 Fragments arranged by size: 3507 1430 1247 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2770 5108 5282 65 Size: 1183 1522 2338 174 1396 Fragments arranged by size: 2338 1522 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5257 5444 1700 Size: 110 3447 187 2869 Fragments arranged by size: 3447 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6613 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6613 BssSI C'ACGA_G Cuts at: 4673 5685 4673 Size: 1012 5601 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6613 BstBI TT'CG_AA Cuts at: 3820 5417 3820 Size: 1597 5016 BstEII G'GTnAC_C Cuts at: 1184 3505 1184 Size: 2321 4292 BstXI CCAn_nnnn'nTGG Cuts at: 2437 3739 2437 Size: 1302 5311 BstYI r'GATC_y Cuts at: 1972 2028 3249 3405 3711 3943 4310 4695 4712 Size: 56 1221 156 306 232 367 385 17 Cuts at: 4712 6164 6250 6262 6608 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1221 385 367 346 306 232 156 86 56 17 12 Bsu36I CC'TnA_GG Cuts at: 2056 2394 3353 3595 3909 2056 Size: 338 959 242 314 4760 Fragments arranged by size: 4760 959 338 314 242 BtgI C'CryG_G Cuts at: 513 1065 2004 2533 3675 3688 3732 3738 513 Size: 552 939 529 1142 13 44 6 3388 Fragments arranged by size: 3388 1142 939 552 529 44 13 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2450 487 Size: 683 56 1224 4650 Fragments arranged by size: 4650 1224 683 56 BtsI GCAGTG_nn' Cuts at: 2838 2941 2966 3442 4129 4923 4943 2838 Size: 103 25 476 687 794 20 4508 Fragments arranged by size: 4508 794 687 476 103 25 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6613 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6578 DraI TTT'AAA Cuts at: 3917 3917 Size: 6613 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2288 5620 809 Size: 507 972 3332 1802 Fragments arranged by size: 3332 1802 972 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2536 2792 3111 Size: 54 1425 50 275 18 706 256 319 Cuts at: 3111 3735 4060 4949 6578 8 Size: 624 325 889 1629 43 Fragments arranged by size: 1629 1425 889 706 624 325 319 275 256 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4060 1537 Size: 275 2248 4090 Fragments arranged by size: 4090 2248 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2148 2549 3462 3572 3779 4543 1353 Size: 795 401 913 110 207 764 3423 Fragments arranged by size: 3423 913 795 764 401 207 110 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5200 5574 5720 387 Size: 1064 138 3611 374 146 1280 Fragments arranged by size: 3611 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2269 2743 3837 2269 Size: 474 1094 5045 Fragments arranged by size: 5045 1094 474 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2516 2591 2690 2942 3080 3293 Size: 393 497 387 75 99 252 138 213 Cuts at: 3293 3494 4676 5273 6060 6328 1239 Size: 201 1182 597 787 268 1524 Fragments arranged by size: 1524 1182 787 597 497 393 387 268 252 213 201 138 99 75 EcoNI CCTnn'n_nnAGG Cuts at: 2090 2090 Size: 6613 EcoO109I rG'GnC_Cy Cuts at: 2447 2689 2872 3268 3345 3346 3642 3905 2447 Size: 242 183 396 77 1 296 263 5155 Fragments arranged by size: 5155 396 296 263 242 183 77 1 EcoRI G'AATT_C Cuts at: 2098 2404 3922 4300 2098 Size: 306 1518 378 4411 Fragments arranged by size: 4411 1518 378 306 FspI TGC'GCA Cuts at: 5119 5119 Size: 6613 HaeII r_GCGC'y Cuts at: 1678 2086 2444 2494 5760 1678 Size: 408 358 50 3266 2531 Fragments arranged by size: 3266 2531 408 358 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2768 2800 3689 3721 3838 Size: 32 135 32 1196 32 889 32 117 Cuts at: 3838 3870 5254 5286 5328 5360 1373 Size: 32 1384 32 42 32 2626 Fragments arranged by size: 2626 1384 1196 889 135 117 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3957 4203 669 Size: 988 281 2019 246 3079 Fragments arranged by size: 3079 2019 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6613 HpaI GTT'AAC Cuts at: 4203 4203 Size: 6613 KpnI G_GTAC'C Cuts at: 3938 3938 Size: 6613 MfeI C'AATT_G Cuts at: 4212 4212 Size: 6613 MluI A'CGCG_T Cuts at: 6489 6489 Size: 6613 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2661 5727 5911 1305 Size: 1356 3066 184 2007 Fragments arranged by size: 3066 2007 1356 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3113 3737 10 Size: 54 1425 343 1281 624 2886 Fragments arranged by size: 2886 1425 1281 624 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2430 2604 3737 4571 4930 5089 518 Size: 648 1264 174 1133 834 359 159 2042 Fragments arranged by size: 2042 1264 1133 834 648 359 174 159 MspA1I CmG'CkG Cuts at: 1606 2701 3154 3208 3256 4029 4706 5854 6099 Size: 1095 453 54 48 773 677 1148 245 Cuts at: 6099 1606 Size: 2120 Fragments arranged by size: 2120 1148 1095 773 677 453 245 54 48 NcoI C'CATG_G Cuts at: 513 1065 2004 3675 3688 3732 3738 513 Size: 552 939 1671 13 44 6 3388 Fragments arranged by size: 3388 1671 939 552 44 13 6 NdeI CA'TA_TG Cuts at: 387 387 Size: 6613 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6613 NotI GC'GGCC_GC Cuts at: 4060 4060 Size: 6613 NspI r_CATG'y Cuts at: 1132 1229 2319 3971 5516 1132 Size: 97 1090 1652 1545 2229 Fragments arranged by size: 2229 1652 1545 1090 97 PasI CC'CwG_GG Cuts at: 1348 2039 1348 Size: 691 5922 PciI A'CATG_T Cuts at: 5512 5512 Size: 6613 PflMI CCAn_nnn'nTGG Cuts at: 1108 2360 3689 3847 1108 Size: 1252 1329 158 3874 Fragments arranged by size: 3874 1329 1252 158 PmeI GTTT'AAAC Cuts at: 3917 3917 Size: 6613 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4682 4714 6195 6227 4682 Size: 32 1481 32 5068 Fragments arranged by size: 5068 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2872 2872 Size: 6613 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6613 PsiI TTA'TAA Cuts at: 4183 4183 Size: 6613 PspOMI G'GGCC_C Cuts at: 2446 3345 2446 Size: 899 5714 PstI C_TGCA'G Cuts at: 830 2490 2631 3201 3498 3965 830 Size: 1660 141 570 297 467 3478 Fragments arranged by size: 3478 1660 570 467 297 141 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6613 PvuII CAG'CTG Cuts at: 3154 3208 3154 Size: 54 6559 SacI G_AGCT'C Cuts at: 2271 2745 3839 2271 Size: 474 1094 5045 Fragments arranged by size: 5045 1094 474 SalI G'TCGA_C Cuts at: 1936 3955 1936 Size: 2019 4594 SapI GCTCTTCn'nnn_ Cuts at: 2549 3462 2549 Size: 913 5700 SbfI CC_TGCA'GG Cuts at: 3201 3965 3201 Size: 764 5849 ScaI AGT'ACT Cuts at: 4861 6573 4861 Size: 1712 4901 SfcI C'TryA_G Cuts at: 826 1045 2486 2627 3197 3494 3870 3961 5096 Size: 219 1441 141 570 297 376 91 1135 Cuts at: 5096 5777 5968 826 Size: 681 191 1471 Fragments arranged by size: 1471 1441 1135 681 570 376 297 219 191 141 91 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6613 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6613 SmaI CCC'GGG Cuts at: 1872 3940 1872 Size: 2068 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2744 4717 5618 5880 820 Size: 197 926 801 1973 901 262 1553 Fragments arranged by size: 1973 1553 926 901 801 262 197 SnaBI TAC'GTA Cuts at: 493 4404 493 Size: 3911 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6613 SphI G_CATG'C Cuts at: 1132 2319 3971 1132 Size: 1187 1652 3774 Fragments arranged by size: 3774 1652 1187 SspI AAT'ATT Cuts at: 5 52 4537 5 Size: 47 4485 2081 Fragments arranged by size: 4485 2081 47 StuI AGG'CCT Cuts at: 1517 3593 3868 1517 Size: 2076 275 4262 Fragments arranged by size: 4262 2076 275 StyI C'CwwG_G Cuts at: 513 1065 2004 2241 2592 2889 3168 3675 3688 Size: 552 939 237 351 297 279 507 13 Cuts at: 3688 3732 3738 4052 513 Size: 44 6 314 3074 Fragments arranged by size: 3074 939 552 507 351 314 297 279 237 44 13 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2564 3155 3639 4640 4657 4810 4995 Size: 1255 984 591 484 1001 17 153 185 Cuts at: 4995 5440 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1001 984 591 484 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4859 6571 96 Size: 274 80 33 51 1443 2882 1712 138 Fragments arranged by size: 2882 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2284 2316 3273 3305 6573 6605 2284 Size: 32 957 32 3268 32 2292 Fragments arranged by size: 3268 2292 957 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6613 XbaI T'CTAG_A Cuts at: 3949 3949 Size: 6613 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3127 3673 3127 Size: 546 6067 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6613 XmaI C'CCGG_G Cuts at: 1870 3938 1870 Size: 2068 4545 XmnI GAAnn'nnTTC Cuts at: 3407 4742 3407 Size: 1335 5278 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4421 276 Size: 53 83 186 936 2887 2468 Fragments arranged by size: 2887 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI