(Circular) (Six-base) MAPSORT of: pFN21AA0100.seq Check: 783 from: 1 to: 11410 pFN21AA0100 11410 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2597 3076 3183 5622 6255 2597 Size: 479 107 2439 633 7752 Fragments arranged by size: 7752 2439 633 479 107 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3830 9220 278 Size: 53 83 186 936 2294 5390 2468 Fragments arranged by size: 5390 2468 2294 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5134 8753 1937 Size: 3197 3619 4594 Fragments arranged by size: 4594 3619 3197 Acc65I G'GTAC_C Cuts at: 5097 8731 5097 Size: 3634 7776 AclI AA'CG_TT Cuts at: 1563 9537 9910 1563 Size: 7974 373 3063 Fragments arranged by size: 7974 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2353 2933 3578 3765 4113 4568 6219 6488 Size: 721 580 645 187 348 455 1651 269 Cuts at: 6488 6550 7218 7562 7957 8573 9473 10857 11125 Size: 62 668 344 395 616 900 1384 268 Cuts at: 11125 1632 Size: 1917 Fragments arranged by size: 1917 1651 1384 900 721 668 645 616 580 455 395 348 344 269 268 187 62 AfeI AGC'GCT Cuts at: 2341 3861 2341 Size: 1520 9890 AflII C'TTAA_G Cuts at: 820 1017 2174 820 Size: 197 1157 10056 Fragments arranged by size: 10056 1157 197 AflIII A'CryG_T Cuts at: 1203 4029 6606 10309 11286 1203 Size: 2826 2577 3703 977 1327 Fragments arranged by size: 3703 2826 2577 1327 977 AgeI A'CCGG_T Cuts at: 10241 10241 Size: 11410 AhdI GACnn_n'nnGTC Cuts at: 10139 10139 Size: 11410 AleI CACnn'nnGTG Cuts at: 1166 2864 5288 5679 1166 Size: 1698 2424 391 6897 Fragments arranged by size: 6897 2424 1698 391 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2673 3023 6128 6924 7463 8208 1184 Size: 1489 350 3105 796 539 745 4386 Fragments arranged by size: 4386 3105 1489 796 745 539 350 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7620 7652 7620 Size: 32 11378 AlwNI CAG_nnn'CTG Cuts at: 2827 2929 3535 4735 5650 7015 8570 9153 10725 Size: 102 606 1200 915 1365 1555 583 1572 Cuts at: 10725 2827 Size: 3512 Fragments arranged by size: 3512 1572 1555 1365 1200 915 606 583 102 ApaI G_GGCC'C Cuts at: 2738 5300 5698 8222 2738 Size: 2562 398 2524 5926 Fragments arranged by size: 5926 2562 2524 398 ApaLI G'TGCA_C Cuts at: 9467 10623 9467 Size: 1156 10254 ApoI r'AATT_y Cuts at: 1493 3155 5246 5826 7287 8719 8945 9097 11206 Size: 1662 2091 580 1461 1432 226 152 2109 Cuts at: 11206 1493 Size: 1697 Fragments arranged by size: 2109 2091 1697 1662 1461 1432 580 226 152 AseI AT'TA_AT Cuts at: 160 1032 9964 160 Size: 872 8932 1606 Fragments arranged by size: 8932 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11410 AvaI C'yCGr_G Cuts at: 1870 1943 6072 8735 1870 Size: 73 4129 2663 4545 Fragments arranged by size: 4545 4129 2663 73 BamHI G'GATC_C Cuts at: 2157 5308 5470 6325 8740 9107 2157 Size: 3151 162 855 2415 367 4460 Fragments arranged by size: 4460 3151 2415 855 367 162 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2895 3147 3451 5097 5144 Size: 325 217 627 1108 252 304 1646 47 Cuts at: 5144 5716 6117 7758 8035 8731 10186 618 Size: 572 401 1641 277 696 1455 1842 Fragments arranged by size: 1842 1646 1641 1455 1108 696 627 572 401 325 304 277 252 217 47 BanII G_rGCy'C Cuts at: 1391 2738 4019 4806 5300 5698 6309 7937 8222 Size: 1347 1281 787 494 398 611 1628 285 Cuts at: 8222 8729 1391 Size: 507 4072 Fragments arranged by size: 4072 1628 1347 1281 787 611 507 494 398 285 BbeI G_GCGC'C Cuts at: 3455 6121 7762 3455 Size: 2666 1641 7103 Fragments arranged by size: 7103 2666 1641 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3747 5396 6058 928 Size: 971 1848 1649 662 6280 Fragments arranged by size: 6280 1848 1649 971 662 BbvCI CC'TCA_GC Cuts at: 4239 5359 6297 6535 7080 4239 Size: 1120 938 238 545 8569 Fragments arranged by size: 8569 1120 938 545 238 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6269 6303 9601 9635 1903 Size: 34 50 34 4248 34 3298 34 3678 Fragments arranged by size: 4248 3678 3298 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2443 7249 9302 10512 2443 Size: 4806 2053 1210 3341 Fragments arranged by size: 4806 3341 2053 1210 BclI T'GATC_A Cuts at: 1548 5671 6211 1548 Size: 4123 540 6747 Fragments arranged by size: 6747 4123 540 BfrBI ATG'CAT Cuts at: 3472 3472 Size: 11410 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 10021 136 Size: 107 122 71 631 605 8349 1525 Fragments arranged by size: 8349 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2388 5020 5184 7225 7410 11405 2388 Size: 2632 164 2041 185 3995 2393 Fragments arranged by size: 3995 2632 2393 2041 185 164 BlpI GC'TnA_GC Cuts at: 2051 3929 4906 6251 8827 2051 Size: 1878 977 1345 2576 4634 Fragments arranged by size: 4634 2576 1878 1345 977 Bme1580I G_kGCm'C Cuts at: 1790 2738 3622 4162 4891 5147 5300 5698 5721 Size: 948 884 540 729 256 153 398 23 Cuts at: 5721 8222 9471 10627 1790 Size: 2501 1249 1156 2573 Fragments arranged by size: 2573 2501 1249 1156 948 884 729 540 398 256 153 23 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 11410 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3547 3917 5509 Size: 416 53 83 399 24 2130 370 1592 Cuts at: 5509 8225 8425 9141 10099 442 Size: 2716 200 716 958 1753 Fragments arranged by size: 2716 2130 1753 1592 958 716 416 399 370 200 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3182 7961 1056 Size: 2126 4779 4505 Fragments arranged by size: 4779 4505 2126 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4651 6325 4651 Size: 1674 9736 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2600 2804 3998 4223 7517 7883 8468 10070 Size: 1361 204 1194 225 3294 366 585 1602 Cuts at: 10070 1239 Size: 2579 Fragments arranged by size: 3294 2579 1602 1361 1194 585 366 225 204 Bpu10I CC'TnA_GC Cuts at: 2237 4164 4239 4386 4956 5359 6297 6535 7080 Size: 1927 75 147 570 403 938 238 545 Cuts at: 7080 7359 7746 8546 2237 Size: 279 387 800 5101 Fragments arranged by size: 5101 1927 938 800 570 545 403 387 279 238 147 75 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2426 3056 5033 6081 7226 7427 9535 10400 10698 Size: 630 1977 1048 1145 201 2108 865 298 Cuts at: 10698 2426 Size: 3138 Fragments arranged by size: 3138 2108 1977 1145 1048 865 630 298 201 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4065 10073 882 Size: 617 2566 6008 2219 Fragments arranged by size: 6008 2566 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 9201 493 Size: 711 7997 2702 Fragments arranged by size: 7997 2702 711 BsaBI GATnn'nnATC Cuts at: 2946 6324 11404 2946 Size: 3378 5080 2952 Fragments arranged by size: 5080 3378 2952 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3393 3452 3827 5179 Size: 53 83 186 936 1860 59 375 1352 Cuts at: 5179 6118 7759 9217 9599 10234 275 Size: 939 1641 1458 382 635 1451 Fragments arranged by size: 1860 1641 1458 1451 1352 939 936 635 382 375 186 83 59 53 BsaWI w'CCGG_w Cuts at: 2085 4410 4440 9843 10241 10515 10662 11073 2085 Size: 2325 30 5403 398 274 147 411 2422 Fragments arranged by size: 5403 2422 2325 411 398 274 147 30 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3971 4001 1178 Size: 30 165 30 2568 30 8587 Fragments arranged by size: 8587 2568 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4448 4814 6314 6317 6992 7442 7883 8636 Size: 3262 366 1500 3 675 450 441 753 Cuts at: 8636 8642 8645 1186 Size: 6 3 3951 Fragments arranged by size: 3951 3262 1500 753 675 450 441 366 6 3 3 BseYI C'CCAG_C Cuts at: 2342 5304 5369 5817 5933 5991 6024 6560 7095 Size: 2962 65 448 116 58 33 536 535 Cuts at: 7095 7300 7783 10613 11109 2342 Size: 205 483 2830 496 2643 Fragments arranged by size: 2962 2830 2643 536 535 496 483 448 205 116 65 58 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3104 3155 3840 4014 4394 5435 6070 Size: 573 1368 51 685 174 380 1041 635 Cuts at: 6070 6335 7028 1163 Size: 265 693 5545 Fragments arranged by size: 5545 1368 1041 693 685 635 573 380 265 174 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8860 9621 10649 1540 Size: 275 186 6859 761 1028 2301 Fragments arranged by size: 6859 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3002 3930 4214 4632 4806 6309 6984 7937 8295 Size: 928 284 418 174 1503 675 953 358 Cuts at: 8295 9471 9556 10627 3002 Size: 1176 85 1071 3785 Fragments arranged by size: 3785 1503 1176 1071 953 928 675 418 358 284 174 85 BsmI GAATG_Cn' Cuts at: 5049 6211 8921 9014 5049 Size: 1162 2710 93 7445 Fragments arranged by size: 7445 2710 1162 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3818 4865 3818 Size: 1047 10363 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2738 3002 3622 3930 4019 4162 4214 Size: 399 948 264 620 308 89 143 52 Cuts at: 4214 4632 4806 4891 5147 5300 5698 5721 6309 Size: 418 174 85 256 153 398 23 588 Cuts at: 6309 6984 7937 8222 8295 8729 9471 9556 10627 Size: 675 953 285 73 434 742 85 1071 Cuts at: 10627 1391 Size: 2174 Fragments arranged by size: 2174 1071 953 948 742 675 620 588 434 418 399 398 308 285 264 256 174 153 143 89 85 85 73 52 23 BspEI T'CCGG_A Cuts at: 4440 11073 4440 Size: 6633 4777 BspHI T'CATG_A Cuts at: 4675 5734 7518 9299 11029 4675 Size: 1059 1784 1781 1730 5056 Fragments arranged by size: 5056 1784 1781 1730 1059 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2597 3076 3183 5457 5622 5649 5989 6255 Size: 1753 479 107 2274 165 27 340 266 Cuts at: 6255 7347 7617 8765 844 Size: 1092 270 1148 3489 Fragments arranged by size: 3489 2274 1753 1148 1092 479 340 270 266 165 107 27 BsrBI CCG'CTC Cuts at: 1394 1823 3972 9297 1394 Size: 429 2149 5325 3507 Fragments arranged by size: 5325 3507 2149 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5062 6396 6690 9905 10079 65 Size: 1183 3814 1334 294 3215 174 1396 Fragments arranged by size: 3814 3215 1396 1334 1183 294 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2070 3144 4284 6984 10054 10241 1700 Size: 110 260 1074 1140 2700 3070 187 2869 Fragments arranged by size: 3070 2869 2700 1140 1074 260 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 11410 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11410 BssSI C'ACGA_G Cuts at: 4569 6315 6546 9470 10482 4569 Size: 1746 231 2924 1012 5497 Fragments arranged by size: 5497 2924 1746 1012 231 BstAPI GCAn_nnn'nTGC Cuts at: 794 3057 794 Size: 2263 9147 BstBI TT'CG_AA Cuts at: 2962 10214 2962 Size: 7252 4158 BstEII G'GTnAC_C Cuts at: 1184 3088 6169 7048 8420 1184 Size: 1904 3081 879 1372 4174 Fragments arranged by size: 4174 3081 1904 1372 879 BstXI CCAn_nnnn'nTGG Cuts at: 2342 5785 6686 7321 2342 Size: 3443 901 635 6431 Fragments arranged by size: 6431 3443 901 635 BstYI r'GATC_y Cuts at: 1972 2157 2388 5020 5109 5184 5308 5470 5788 Size: 185 231 2632 89 75 124 162 318 Cuts at: 5788 6325 7225 7410 8740 9107 9492 9509 10961 Size: 537 900 185 1330 367 385 17 1452 Cuts at: 10961 11047 11059 11405 1972 Size: 86 12 346 1977 Fragments arranged by size: 2632 1977 1452 1330 900 537 385 367 346 318 231 185 185 162 124 89 86 75 17 12 Bsu36I CC'TnA_GG Cuts at: 3365 5010 5712 3365 Size: 1645 702 9063 Fragments arranged by size: 9063 1645 702 BtgI C'CryG_G Cuts at: 513 1065 4948 5093 5204 5413 5656 8487 513 Size: 552 3883 145 111 209 243 2831 3436 Fragments arranged by size: 3883 3436 2831 552 243 209 145 111 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 10671 Fragments arranged by size: 10671 683 56 BtsI GCAGTG_nn' Cuts at: 2655 3436 3823 4889 5226 5651 6830 7041 8926 Size: 781 387 1066 337 425 1179 211 1885 Cuts at: 8926 9720 9740 2655 Size: 794 20 4325 Fragments arranged by size: 4325 1885 1179 1066 794 781 425 387 337 211 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 11410 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 11375 DraI TTT'AAA Cuts at: 8714 8714 Size: 11410 DraIII CAC_nnn'GTG Cuts at: 2100 2260 6548 8299 8381 2100 Size: 160 4288 1751 82 5129 Fragments arranged by size: 5129 4288 1751 160 82 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3734 10417 809 Size: 507 2418 6683 1802 Fragments arranged by size: 6683 2418 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 4053 4749 Size: 54 1425 50 275 18 261 1962 696 Cuts at: 4749 5329 5460 6943 8098 8857 9746 11375 8 Size: 580 131 1483 1155 759 889 1629 43 Fragments arranged by size: 1962 1629 1483 1425 1155 889 759 696 580 275 261 131 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8857 1537 Size: 275 7045 4090 Fragments arranged by size: 7045 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2714 3694 4389 5921 6195 6621 6651 7976 Size: 1361 980 695 1532 274 426 30 1325 Cuts at: 7976 8127 8619 9340 1353 Size: 151 492 721 3423 Fragments arranged by size: 3423 1532 1361 1325 980 721 695 492 426 274 151 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2138 7955 9997 10371 10517 387 Size: 1064 138 549 5817 2042 374 146 1280 Fragments arranged by size: 5817 2042 1280 1064 549 374 146 138 EcoICRI GAG'CTC Cuts at: 4804 6307 7935 4804 Size: 1503 1628 8279 Fragments arranged by size: 8279 1628 1503 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2353 2600 2804 2933 3578 3765 3998 Size: 393 721 247 204 129 645 187 233 Cuts at: 3998 4113 4223 4568 6219 6488 6550 7218 7517 Size: 115 110 345 1651 269 62 668 299 Cuts at: 7517 7562 7883 7957 8468 8573 9473 10070 10857 Size: 45 321 74 511 105 900 597 787 Cuts at: 10857 11125 1239 Size: 268 1524 Fragments arranged by size: 1651 1524 900 787 721 668 645 597 511 393 345 321 299 269 268 247 233 204 187 129 115 110 105 74 62 45 EcoNI CCTnn'n_nnAGG Cuts at: 5711 6099 6164 7766 5711 Size: 388 65 1602 9355 Fragments arranged by size: 9355 1602 388 65 EcoO109I rG'GnC_Cy Cuts at: 2704 2734 3904 5296 5695 6463 8219 2704 Size: 30 1170 1392 399 768 1756 5895 Fragments arranged by size: 5895 1756 1392 1170 768 399 30 EcoRI G'AATT_C Cuts at: 3155 5826 8719 9097 3155 Size: 2671 2893 378 5468 Fragments arranged by size: 5468 2893 2671 378 EcoRV GAT'ATC Cuts at: 5566 5566 Size: 11410 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3745 5575 8710 3745 Size: 1830 3135 6445 Fragments arranged by size: 6445 3135 1830 FspI TGC'GCA Cuts at: 9916 9916 Size: 11410 HaeII r_GCGC'y Cuts at: 1678 2058 2343 3455 3863 6121 7762 10557 1678 Size: 380 285 1112 408 2258 1641 2795 2531 Fragments arranged by size: 2795 2531 2258 1641 1112 408 380 285 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2893 2925 4259 4291 5249 Size: 32 135 32 1321 32 1334 32 958 Cuts at: 5249 5281 6572 6604 8126 8158 10051 10083 10125 Size: 32 1291 32 1522 32 1893 32 42 Cuts at: 10125 10157 1373 Size: 32 2626 Fragments arranged by size: 2626 1893 1522 1334 1321 1291 958 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3289 5135 6553 6748 7333 8754 Size: 988 281 1351 1846 1418 195 585 1421 Cuts at: 8754 9000 669 Size: 246 3079 Fragments arranged by size: 3079 1846 1421 1418 1351 988 585 281 246 195 HindIII A'AGCT_T Cuts at: 748 2965 7574 748 Size: 2217 4609 4584 Fragments arranged by size: 4609 4584 2217 HpaI GTT'AAC Cuts at: 6553 9000 6553 Size: 2447 8963 KasI G'GCGC_C Cuts at: 3451 6117 7758 3451 Size: 2666 1641 7103 Fragments arranged by size: 7103 2666 1641 KpnI G_GTAC'C Cuts at: 5101 8735 5101 Size: 3634 7776 MfeI C'AATT_G Cuts at: 9009 9009 Size: 11410 MluI A'CGCG_T Cuts at: 11286 11286 Size: 11410 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7687 10524 10708 1305 Size: 6382 2837 184 2007 Fragments arranged by size: 6382 2837 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2093 4055 5331 5462 10 Size: 54 1425 343 261 1962 1276 131 5958 Fragments arranged by size: 5958 1962 1425 1276 343 261 131 54 MslI CAynn'nnrTG Cuts at: 518 1166 2418 2864 2940 3715 4877 5288 5679 Size: 648 1252 446 76 775 1162 411 391 Cuts at: 5679 5783 6110 8470 9368 9727 9886 518 Size: 104 327 2360 898 359 159 2042 Fragments arranged by size: 2360 2042 1252 1162 898 775 648 446 411 391 359 327 159 104 76 MspA1I CmG'CkG Cuts at: 1606 2110 2578 2827 2953 3388 3448 3674 4378 Size: 504 468 249 126 435 60 226 704 Cuts at: 4378 5363 5458 5995 6085 6296 6349 6434 6803 Size: 985 95 537 90 211 53 85 369 Cuts at: 6803 7079 7108 7495 7615 7672 7783 8053 8188 Size: 276 29 387 120 57 111 270 135 Cuts at: 8188 8826 9503 10651 10896 1606 Size: 638 677 1148 245 2120 Fragments arranged by size: 2120 1148 985 704 677 638 537 504 468 435 387 369 276 270 249 245 226 211 135 126 120 111 95 90 85 60 57 53 29 NaeI GCC'GGC Cuts at: 4286 4286 Size: 11410 NarI GG'CG_CC Cuts at: 3452 6118 7759 3452 Size: 2666 1641 7103 Fragments arranged by size: 7103 2666 1641 NcoI C'CATG_G Cuts at: 513 1065 4948 5413 5656 8487 513 Size: 552 3883 465 243 2831 3436 Fragments arranged by size: 3883 3436 2831 552 465 243 NdeI CA'TA_TG Cuts at: 387 387 Size: 11410 NgoMIV G'CCGG_C Cuts at: 4284 4284 Size: 11410 NheI G'CTAG_C Cuts at: 1052 3178 7957 1052 Size: 2126 4779 4505 Fragments arranged by size: 4779 4505 2126 NotI GC'GGCC_GC Cuts at: 8857 8857 Size: 11410 NsiI A_TGCA'T Cuts at: 3474 3474 Size: 11410 NspI r_CATG'y Cuts at: 1132 1229 3476 4033 4049 4964 5236 6610 6901 Size: 97 2247 557 16 915 272 1374 291 Cuts at: 6901 7478 8614 8768 10313 1132 Size: 577 1136 154 1545 2229 Fragments arranged by size: 2247 2229 1545 1374 1136 915 577 557 291 272 154 97 16 PasI CC'CwG_GG Cuts at: 1348 8222 1348 Size: 6874 4536 PciI A'CATG_T Cuts at: 4029 6606 10309 4029 Size: 2577 3703 5130 Fragments arranged by size: 5130 3703 2577 PflMI CCAn_nnn'nTGG Cuts at: 1108 2100 3250 3535 3662 5338 7065 7282 1108 Size: 992 1150 285 127 1676 1727 217 5236 Fragments arranged by size: 5236 1727 1676 1150 992 285 217 127 PfoI T'CCnGG_A Cuts at: 6374 6875 7320 6374 Size: 501 445 10464 Fragments arranged by size: 10464 501 445 PmeI GTTT'AAAC Cuts at: 8714 8714 Size: 11410 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4232 4264 9479 9511 10992 11024 4232 Size: 32 5215 32 1481 32 4618 Fragments arranged by size: 5215 4618 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2622 5885 6162 1613 Size: 1009 3263 277 6861 Fragments arranged by size: 6861 3263 1009 277 PsiI TTA'TAA Cuts at: 8980 8980 Size: 11410 PspOMI G'GGCC_C Cuts at: 2734 5296 5694 8218 2734 Size: 2562 398 2524 5926 Fragments arranged by size: 5926 2562 2524 398 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4959 4991 4959 Size: 32 11378 PstI C_TGCA'G Cuts at: 830 2121 2925 4267 4305 4434 5646 5906 6000 Size: 1291 804 1342 38 129 1212 260 94 Cuts at: 6000 6825 7614 7671 7809 8762 830 Size: 825 789 57 138 953 3478 Fragments arranged by size: 3478 1342 1291 1212 953 825 804 789 260 138 129 94 57 38 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11410 PvuII CAG'CTG Cuts at: 2578 2827 2953 3674 4378 5363 5458 5995 6296 Size: 249 126 721 704 985 95 537 301 Cuts at: 6296 6349 6434 6803 7079 7495 7615 7783 8053 Size: 53 85 369 276 416 120 168 270 Cuts at: 8053 8188 2578 Size: 135 5800 Fragments arranged by size: 5800 985 721 704 537 416 369 301 276 270 249 168 135 126 120 95 85 53 SacI G_AGCT'C Cuts at: 4806 6309 7937 4806 Size: 1503 1628 8279 Fragments arranged by size: 8279 1628 1503 SalI G'TCGA_C Cuts at: 1936 5133 8752 1936 Size: 3197 3619 4594 Fragments arranged by size: 4594 3619 3197 SapI GCTCTTCn'nnn_ Cuts at: 5921 5921 Size: 11410 SbfI CC_TGCA'GG Cuts at: 4305 8762 4305 Size: 4457 6953 ScaI AGT'ACT Cuts at: 6905 7920 9658 11370 6905 Size: 1015 1738 1712 6945 Fragments arranged by size: 6945 1738 1712 1015 SexAI A'CCwGG_T Cuts at: 2835 5533 8430 2835 Size: 2698 2897 5815 Fragments arranged by size: 5815 2897 2698 SfcI C'TryA_G Cuts at: 826 1045 2117 2300 2921 3512 4061 4263 4301 Size: 219 1072 183 621 591 549 202 38 Cuts at: 4301 4430 4727 5642 5902 5996 6821 7610 7667 Size: 129 297 915 260 94 825 789 57 Cuts at: 7667 7805 7819 8758 9893 10574 10765 826 Size: 138 14 939 1135 681 191 1471 Fragments arranged by size: 1471 1135 1072 939 915 825 789 681 621 591 549 297 260 219 202 191 183 138 129 94 57 38 14 SfoI GGC'GCC Cuts at: 3453 6119 7760 3453 Size: 2666 1641 7103 Fragments arranged by size: 7103 2666 1641 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11410 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11410 SmaI CCC'GGG Cuts at: 1872 8737 1872 Size: 6865 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2174 2441 3071 5048 6096 7205 Size: 197 926 231 267 630 1977 1048 1109 Cuts at: 7205 7406 9514 10415 10677 820 Size: 201 2108 901 262 1553 Fragments arranged by size: 2108 1977 1553 1109 1048 926 901 630 267 262 231 201 197 SnaBI TAC'GTA Cuts at: 493 9201 493 Size: 8708 2702 SpeI A'CTAG_T Cuts at: 152 6527 152 Size: 6375 5035 SphI G_CATG'C Cuts at: 1132 6901 8768 1132 Size: 5769 1867 3774 Fragments arranged by size: 5769 3774 1867 SspI AAT'ATT Cuts at: 5 52 5274 9334 5 Size: 47 5222 4060 2081 Fragments arranged by size: 5222 4060 2081 47 StuI AGG'CCT Cuts at: 1517 5896 1517 Size: 4379 7031 StyI C'CwwG_G Cuts at: 513 1065 3193 3327 3768 4948 5413 5656 5721 Size: 552 2128 134 441 1180 465 243 65 Cuts at: 5721 6228 8487 8849 513 Size: 507 2259 362 3074 Fragments arranged by size: 3074 2259 2128 1180 552 507 465 441 362 243 134 65 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3474 3478 3566 4891 6208 9437 9454 Size: 1255 1894 4 88 1325 1317 3229 17 Cuts at: 9454 9607 9792 10237 325 Size: 153 185 445 1498 Fragments arranged by size: 3229 1894 1498 1325 1317 1255 445 185 153 88 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5280 6436 6903 Size: 274 80 33 51 1443 3303 1156 467 Cuts at: 6903 7137 7918 9656 11368 96 Size: 234 781 1738 1712 138 Fragments arranged by size: 3303 1738 1712 1443 1156 781 467 274 234 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3363 3395 3582 3614 3971 4003 4078 4110 5455 Size: 32 187 32 357 32 75 32 1345 Cuts at: 5455 5487 5630 5662 8154 8186 11370 11402 3363 Size: 32 143 32 2492 32 3184 32 3371 Fragments arranged by size: 3371 3184 2492 1345 357 187 143 75 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2837 7328 1316 Size: 1521 4491 5398 Fragments arranged by size: 5398 4491 1521 XbaI T'CTAG_A Cuts at: 4516 8746 4516 Size: 4230 7180 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5382 7612 5382 Size: 2230 9180 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 11410 XmaI C'CCGG_G Cuts at: 1870 8735 1870 Size: 6865 4545 XmnI GAAnn'nnTTC Cuts at: 2390 4138 4418 4974 7857 9539 2390 Size: 1748 280 556 2883 1682 4261 Fragments arranged by size: 4261 2883 1748 1682 556 280 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3828 9218 276 Size: 53 83 186 936 2294 5390 2468 Fragments arranged by size: 5390 2468 2294 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BsiWI BstZ17I FseI FspAI NruI PacI PmlI PpuMI PspXI RsrII SacII SanDI SfiI SrfI SwaI