Restriction map for
pFN21AA0041
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AccII AclI
AflII AgeI AhdI Aor51HI ApaI
AscI Asp718I AspEI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BclI BcnI BfrI BlnI BsaAI
BsaBI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstXI BstZ17I
Cfr10I ClaI CpoI DraII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoT22I
FbaI FseI FspI HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MscI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI StuI SwaI Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 1312 TCCGGA T^CCGGA
AcyI 1836 GGCGTC G[GA]^CG[CT]C
AflIII 1966 ACGTGT A^C[GA][CT]GT
Alw44I 662 GTGCAC G^TGCAC
AlwNI 855 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 936 CCTCAGG CC^T[ACGT]AGG
ApaLI 662 GTGCAC G^TGCAC
AseI 529 ATTAAT AT^TAAT
AsnI 529 ATTAAT AT^TAAT
Asp700I 372 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1642 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 2089 GGTGCC G^G[CT][GA]CC
BbvCI 1867 GCTGAGG CCTCAGC(-5/-2)
BcgI 976 CGACGATTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1220 GTATCC GTATCC(6/5)
BglI 592 GCCCATTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 345 AGATCT A^GATCT
BlpI 1614 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1614 GCTGAGC GC^T[ACGT]AGC
BsaHI 1836 GGCGTC G[GA]^CG[CT]C
BseAI 1312 TCCGGA T^CCGGA
BseRI 22 GAGGAG GAGGAG(10/8)
BsgI 1886 GTGCAG GTGCAG(16/14)
BspEI 1312 TCCGGA T^CCGGA
BspHI 176 TCATGA T^CATGA
BspMI 2064 GCAGGT ACCTGC(4/8)
BstAPI 2149 GCAGCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 379 TTCGAA TT^CGAA
Bsu36I 936 CCTCAGG CC^T[ACGT]AGG
CelII 1614 GCTGAGC GC^T[ACGT]AGC
DraI 1389 TTTAAA TTT^AAA
DraIII 1293 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 936 CCTCAGG CC^T[ACGT]AGG
EcoRV 812 GATATC GAT^ATC
HaeII 857 GGCGCT [GA]GCGC^[CT]
HgaI 1837 GCGTC GACGC(5/10)
Hin1I 1836 GGCGTC G[GA]^CG[CT]C
HincII 1999 GTCAAC GT[CT]^[GA]AC
HindII 1999 GTCAAC GT[CT]^[GA]AC
MroI 1312 TCCGGA T^CCGGA
NdeI 1841 CATATG CA^TATG
NspV 379 TTCGAA TT^CGAA
PflFI 1642 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 529 ATTAAT AT^TAAT
PvuII 1236 CAGCTG CAG^CTG
RcaI 176 TCATGA T^CATGA
SacI 1701 GAGCTC GAGCT^C
SfcI 1981 CTACAG C^T[GA][CT]AG
SfuI 379 TTCGAA TT^CGAA
SphI 1016 GCATGC GCATG^C
SspI 1544 AATATT AAT^ATT
Tth111I 1642 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 372 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 430 GTAC GT^AC
1351 GTAC
BanII 1701 GAGCTC G[GA]GC[CT]^C
1953 GGGCTC
BfaI 209 CTAG C^TAG
1153 CTAG
BpmI 750 CTCCAG CTGGAG(16/14)
1021 CTCCAG
BsaI 191 GAGACC GGTCTC(1/5)
996 GGTCTC
BsaWI 1312 TCCGGA [AT]^CCGG[AT]
1322 ACCGGA
BsmFI 1640 GGGAC GGGAC(10/14)
2025 GGGAC
DsaI 1264 CCACGG C^C[GA][CT]GG
1733 CCACGG
EarI 1715 GAAGAG CTCTTC(1/4)
2197 GAAGAG
EcoRI 1316 GAATTC G^AATTC
1691 GAATTC
EcoT14I 1329 CCTTGG C^C[AT][AT]GG
2016 CCAAGG
HapII 1313 CCGG C^CGG
1323 CCGG
HindIII 109 AAGCTT A^AGCTT
1393 AAGCTT
HpaII 1313 CCGG C^CGG
1323 CCGG
Ksp632I 1715 GAAGAG CTCTTC(1/4)
2197 GAAGAG
MaeI 209 CTAG C^TAG
1153 CTAG
MslI 798 CATAGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2136 CATAGCTGTG
MspA1I 1207 CAGCGG C[AC]G^C[GT]G
1236 CAGCTG
MspI 1313 CCGG C^CGG
1323 CCGG
NspI 1016 GCATGC [GA]CATG^[CT]
2100 ACATGC
RsaI 430 GTAC GT^AC
1351 GTAC
StyI 1329 CCTTGG C^C[AT][AT]GG
2016 CCAAGG
XcmI 1215 CCAGTGTATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1274 CCAGCATCAACCTGG
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI AvaII BbsI BbvI BmyI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII