(Circular) (Six-base) MAPSORT of: pFN21AA0041.seq Check: 7614 from: 1 to: 7039 pFN21AA0041 7039 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4849 278 Size: 53 83 186 936 3313 2468 Fragments arranged by size: 3313 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4382 1937 Size: 2445 4594 Acc65I G'GTAC_C Cuts at: 4360 4360 Size: 7039 AclI AA'CG_TT Cuts at: 1563 5166 5539 1563 Size: 3603 373 3063 Fragments arranged by size: 3603 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2038 2057 2315 2513 3194 3627 3789 3812 Size: 406 19 258 198 681 433 162 23 Cuts at: 3812 5102 6486 6754 1632 Size: 1290 1384 268 1917 Fragments arranged by size: 1917 1384 1290 681 433 406 268 258 198 162 23 19 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6842 AflIII A'CryG_T Cuts at: 1203 3971 5938 6915 1203 Size: 2768 1967 977 1327 Fragments arranged by size: 2768 1967 1327 977 AgeI A'CCGG_T Cuts at: 5870 5870 Size: 7039 AhdI GACnn_n'nnGTC Cuts at: 5768 5768 Size: 7039 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7039 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7039 AlwNI CAG_nnn'CTG Cuts at: 2865 4782 6354 2865 Size: 1917 1572 3550 Fragments arranged by size: 3550 1917 1572 ApaLI G'TGCA_C Cuts at: 2667 5096 6252 2667 Size: 2429 1156 3454 Fragments arranged by size: 3454 2429 1156 ApoI r'AATT_y Cuts at: 1493 2278 2360 2906 3321 3536 3632 3696 4334 Size: 785 82 546 415 215 96 64 638 Cuts at: 4334 4348 4574 4726 6835 1493 Size: 14 226 152 2109 1697 Fragments arranged by size: 2109 1697 785 638 546 415 226 215 152 96 82 64 14 AseI AT'TA_AT Cuts at: 160 1032 2535 5593 160 Size: 872 1503 3058 1606 Fragments arranged by size: 3058 1606 1503 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7039 AvaI C'yCGr_G Cuts at: 1870 1943 4364 1870 Size: 73 2421 4545 Fragments arranged by size: 4545 2421 73 BamHI G'GATC_C Cuts at: 4369 4736 4369 Size: 367 6672 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4094 4360 5815 618 Size: 325 217 627 2307 266 1455 1842 Fragments arranged by size: 2307 1842 1455 627 325 266 217 BanII G_rGCy'C Cuts at: 1391 3710 3962 4358 1391 Size: 2319 252 396 4072 Fragments arranged by size: 4072 2319 396 252 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2255 2943 2976 928 Size: 971 356 688 33 4991 Fragments arranged by size: 4991 971 688 356 33 BbvCI CC'TCA_GC Cuts at: 3873 3873 Size: 7039 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2970 3004 5230 5264 1903 Size: 34 1033 34 2226 34 3678 Fragments arranged by size: 3678 2226 1033 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3236 4931 6141 3236 Size: 1695 1210 4134 Fragments arranged by size: 4134 1695 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7039 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2603 5650 136 Size: 107 122 71 631 605 931 3047 1525 Fragments arranged by size: 3047 1525 931 631 605 122 107 71 BglII A'GATC_T Cuts at: 2350 7034 2350 Size: 4684 2355 BlpI GC'TnA_GC Cuts at: 3620 4456 3620 Size: 836 6203 Bme1580I G_kGCm'C Cuts at: 1790 2671 4062 5100 6256 1790 Size: 881 1391 1038 1156 2573 Fragments arranged by size: 2573 1391 1156 1038 881 BmgBI CAC'GTC Cuts at: 1318 3898 3972 1318 Size: 2580 74 4385 Fragments arranged by size: 4385 2580 74 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3913 4770 5728 Size: 416 53 83 399 24 2496 857 958 Cuts at: 5728 442 Size: 1753 Fragments arranged by size: 2496 1753 958 857 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7039 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3019 3019 Size: 7039 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2740 3011 5699 1239 Size: 1501 271 2688 2579 Fragments arranged by size: 2688 2579 1501 271 Bpu10I CC'TnA_GC Cuts at: 3573 3873 3573 Size: 300 6739 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5164 6029 6327 5164 Size: 865 298 5876 Fragments arranged by size: 5876 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2190 3007 5702 882 Size: 617 691 817 2695 2219 Fragments arranged by size: 2695 2219 817 691 617 BsaAI yAC'GTr Cuts at: 493 1204 4830 493 Size: 711 3626 2702 Fragments arranged by size: 3626 2702 711 BsaBI GATnn'nnATC Cuts at: 7033 7033 Size: 7039 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3842 4846 5228 5863 Size: 53 83 186 936 2309 1004 382 635 Cuts at: 5863 275 Size: 1451 Fragments arranged by size: 2309 1451 1004 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3317 3327 5472 5870 6144 6291 6702 3317 Size: 10 2145 398 274 147 411 3654 Fragments arranged by size: 3654 2145 411 398 274 147 10 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6814 Fragments arranged by size: 6814 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 1186 Size: 856 6183 BseYI C'CCAG_C Cuts at: 6242 6738 6242 Size: 496 6543 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3912 1163 Size: 573 2176 4290 Fragments arranged by size: 4290 2176 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4489 5250 6278 1540 Size: 275 186 2488 761 1028 2301 Fragments arranged by size: 2488 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2671 3317 3710 5100 5185 6256 2671 Size: 646 393 1390 85 1071 3454 Fragments arranged by size: 3454 1390 1071 646 393 85 BsmI GAATG_Cn' Cuts at: 4550 4643 4550 Size: 93 6946 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2671 3317 3710 3962 4062 4358 5100 Size: 399 881 646 393 252 100 296 742 Cuts at: 5100 5185 6256 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 881 742 646 399 393 296 252 100 85 BspEI T'CCGG_A Cuts at: 3317 6702 3317 Size: 3385 3654 BspHI T'CATG_A Cuts at: 2181 4928 6658 2181 Size: 2747 1730 2562 Fragments arranged by size: 2747 2562 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4060 4394 844 Size: 3216 334 3489 Fragments arranged by size: 3489 3216 334 BsrBI CCG'CTC Cuts at: 1394 1823 4926 1394 Size: 429 3103 3507 Fragments arranged by size: 3507 3103 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2142 2845 3241 4034 5534 5708 65 Size: 1183 894 703 396 793 1500 174 1396 Fragments arranged by size: 1500 1396 1183 894 793 703 396 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5683 5870 1700 Size: 110 3873 187 2869 Fragments arranged by size: 3873 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7039 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7039 BssSI C'ACGA_G Cuts at: 5099 6111 5099 Size: 1012 6027 BstAPI GCAn_nnn'nTGC Cuts at: 794 4160 794 Size: 3366 3673 BstBI TT'CG_AA Cuts at: 2385 5843 2385 Size: 3458 3581 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7039 BstYI r'GATC_y Cuts at: 1972 2350 2659 3265 4369 4736 5121 5138 6590 Size: 378 309 606 1104 367 385 17 1452 Cuts at: 6590 6676 6688 7034 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1104 606 385 378 367 346 309 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2942 2942 Size: 7039 BtgI C'CryG_G Cuts at: 513 1065 3269 3738 513 Size: 552 2204 469 3814 Fragments arranged by size: 3814 2204 552 469 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6300 Fragments arranged by size: 6300 683 56 BtsI GCAGTG_nn' Cuts at: 4555 5349 5369 4555 Size: 794 20 6225 Fragments arranged by size: 6225 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7039 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3507 3542 529 Size: 35 2943 35 4026 Fragments arranged by size: 4026 2943 35 35 DraI TTT'AAA Cuts at: 3396 4343 3396 Size: 947 6092 DraIII CAC_nnn'GTG Cuts at: 3303 3303 Size: 7039 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6046 809 Size: 507 4730 1802 Fragments arranged by size: 4730 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4486 5375 7004 Size: 54 1425 50 275 18 2656 889 1629 Cuts at: 7004 8 Size: 43 Fragments arranged by size: 2656 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4486 1537 Size: 275 2674 4090 Fragments arranged by size: 4090 2674 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3715 4197 4969 1353 Size: 2362 482 772 3423 Fragments arranged by size: 3423 2362 772 482 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5626 6000 6146 387 Size: 1064 138 4037 374 146 1280 Fragments arranged by size: 4037 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3708 3708 Size: 7039 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2038 2057 2315 2513 2740 3011 3194 Size: 393 406 19 258 198 227 271 183 Cuts at: 3194 3627 3789 3812 5102 5699 6486 6754 1239 Size: 433 162 23 1290 597 787 268 1524 Fragments arranged by size: 1524 1290 787 597 433 406 393 271 268 258 227 198 183 162 23 19 EcoRI G'AATT_C Cuts at: 3321 3696 4348 4726 3321 Size: 375 652 378 5634 Fragments arranged by size: 5634 652 378 375 EcoRV GAT'ATC Cuts at: 2819 2819 Size: 7039 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2369 3744 2369 Size: 1375 5664 FspI TGC'GCA Cuts at: 5545 5545 Size: 7039 HaeII r_GCGC'y Cuts at: 1678 2866 6186 1678 Size: 1188 3320 2531 Fragments arranged by size: 3320 2531 1188 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2550 2582 3119 3151 5680 Size: 32 135 32 978 32 537 32 2529 Cuts at: 5680 5712 5754 5786 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2529 978 537 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4006 4383 4629 669 Size: 988 281 2068 377 246 3079 Fragments arranged by size: 3079 2068 988 377 281 246 HindIII A'AGCT_T Cuts at: 748 2114 3398 748 Size: 1366 1284 4389 Fragments arranged by size: 4389 1366 1284 HpaI GTT'AAC Cuts at: 4629 4629 Size: 7039 KpnI G_GTAC'C Cuts at: 4364 4364 Size: 7039 MfeI C'AATT_G Cuts at: 4638 4638 Size: 7039 MluI A'CGCG_T Cuts at: 6915 6915 Size: 7039 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2527 2655 2945 6153 6337 1305 Size: 1222 128 290 3208 184 2007 Fragments arranged by size: 3208 2007 1222 290 184 128 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5217 Fragments arranged by size: 5217 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2807 4145 4997 5356 5515 518 Size: 648 843 798 1338 852 359 159 2042 Fragments arranged by size: 2042 1338 852 843 798 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3214 3243 4455 5132 6280 6525 1606 Size: 1608 29 1212 677 1148 245 2120 Fragments arranged by size: 2120 1608 1212 1148 677 245 29 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6487 NdeI CA'TA_TG Cuts at: 387 3847 387 Size: 3460 3579 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7039 NotI GC'GGCC_GC Cuts at: 4486 4486 Size: 7039 NspI r_CATG'y Cuts at: 1132 1229 3025 4109 4397 5942 1132 Size: 97 1796 1084 288 1545 2229 Fragments arranged by size: 2229 1796 1545 1084 288 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7039 PciI A'CATG_T Cuts at: 5938 5938 Size: 7039 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7039 PfoI T'CCnGG_A Cuts at: 3820 3820 Size: 7039 PmeI GTTT'AAAC Cuts at: 4343 4343 Size: 7039 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5108 5140 6621 6653 5108 Size: 32 1481 32 5494 Fragments arranged by size: 5494 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7039 PsiI TTA'TAA Cuts at: 3428 4609 3428 Size: 1181 5858 PstI C_TGCA'G Cuts at: 830 4391 830 Size: 3561 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7039 PvuII CAG'CTG Cuts at: 3243 3243 Size: 7039 SacI G_AGCT'C Cuts at: 3710 3710 Size: 7039 SalI G'TCGA_C Cuts at: 1936 4381 1936 Size: 2445 4594 SbfI CC_TGCA'GG Cuts at: 4391 4391 Size: 7039 ScaI AGT'ACT Cuts at: 5287 6999 5287 Size: 1712 5327 SfcI C'TryA_G Cuts at: 826 1045 3986 4387 5522 6203 6394 826 Size: 219 2941 401 1135 681 191 1471 Fragments arranged by size: 2941 1471 1135 681 401 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7039 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7039 SmaI CCC'GGG Cuts at: 1872 4366 1872 Size: 2494 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5143 6044 6306 820 Size: 197 926 3200 901 262 1553 Fragments arranged by size: 3200 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4830 493 Size: 4337 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7039 SphI G_CATG'C Cuts at: 1132 3025 4397 1132 Size: 1893 1372 3774 Fragments arranged by size: 3774 1893 1372 SspI AAT'ATT Cuts at: 5 52 3551 4963 5 Size: 47 3499 1412 2081 Fragments arranged by size: 3499 2081 1412 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7039 StyI C'CwwG_G Cuts at: 513 1065 3334 4021 4478 513 Size: 552 2269 687 457 3074 Fragments arranged by size: 3074 2269 687 552 457 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5066 5083 5236 5421 5866 325 Size: 1255 3486 17 153 185 445 1498 Fragments arranged by size: 3486 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2434 5285 6997 Size: 274 80 33 51 1443 457 2851 1712 Cuts at: 6997 96 Size: 138 Fragments arranged by size: 2851 1712 1443 457 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6999 7031 6999 Size: 32 7007 Tth111I GACn'n_nGTC Cuts at: 1316 3650 1316 Size: 2334 4705 XbaI T'CTAG_A Cuts at: 4375 4375 Size: 7039 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3227 3286 3227 Size: 59 6980 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7039 XmaI C'CCGG_G Cuts at: 1870 4364 1870 Size: 2494 4545 XmnI GAAnn'nnTTC Cuts at: 2381 5168 2381 Size: 2787 4252 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4847 276 Size: 53 83 186 936 3313 2468 Fragments arranged by size: 3313 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI