(Circular) (Six-base) MAPSORT of: pFN21AA0029.seq Check: 3980 from: 1 to: 7618 pFN21AA0029 7618 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5428 278 Size: 53 83 186 936 3892 2468 Fragments arranged by size: 3892 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4236 4961 1937 Size: 2299 725 4594 Fragments arranged by size: 4594 2299 725 Acc65I G'GTAC_C Cuts at: 4939 4939 Size: 7618 AclI AA'CG_TT Cuts at: 1563 2629 5745 6118 1563 Size: 1066 3116 373 3063 Fragments arranged by size: 3116 3063 1066 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2865 3001 3329 3789 3963 4847 5681 7065 Size: 1233 136 328 460 174 884 834 1384 Cuts at: 7065 7333 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1233 884 834 460 328 268 174 136 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7421 AflIII A'CryG_T Cuts at: 1203 6517 7494 1203 Size: 5314 977 1327 Fragments arranged by size: 5314 1327 977 AgeI A'CCGG_T Cuts at: 6449 6449 Size: 7618 AhdI GACnn_n'nnGTC Cuts at: 4382 4684 6347 4382 Size: 302 1663 5653 Fragments arranged by size: 5653 1663 302 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7618 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7618 AlwNI CAG_nnn'CTG Cuts at: 4429 5361 6933 4429 Size: 932 1572 5114 Fragments arranged by size: 5114 1572 932 ApaLI G'TGCA_C Cuts at: 5675 6831 5675 Size: 1156 6462 ApoI r'AATT_y Cuts at: 1493 2181 2336 2403 2444 2582 3849 4223 4927 Size: 688 155 67 41 138 1267 374 704 Cuts at: 4927 5153 5305 7414 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1267 704 688 374 226 155 152 138 67 41 AseI AT'TA_AT Cuts at: 160 1032 6172 160 Size: 872 5140 1606 Fragments arranged by size: 5140 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7618 AvaI C'yCGr_G Cuts at: 1870 1943 4388 4943 1870 Size: 73 2445 555 4545 Fragments arranged by size: 4545 2445 555 73 AvrII C'CTAG_G Cuts at: 3250 3250 Size: 7618 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3578 3611 3578 Size: 33 7585 BamHI G'GATC_C Cuts at: 4948 5315 4948 Size: 367 7251 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2850 4655 4939 6394 618 Size: 325 217 627 1063 1805 284 1455 1842 Fragments arranged by size: 1842 1805 1455 1063 627 325 284 217 BanII G_rGCy'C Cuts at: 1391 4937 1391 Size: 3546 4072 BbeI G_GCGC'C Cuts at: 2854 4659 2854 Size: 1805 5813 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2622 928 Size: 971 723 5924 Fragments arranged by size: 5924 971 723 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5809 5843 1903 Size: 34 3872 34 3678 Fragments arranged by size: 3872 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4419 5510 6720 4419 Size: 1091 1210 5317 Fragments arranged by size: 5317 1210 1091 BclI T'GATC_A Cuts at: 1548 2521 2575 4195 1548 Size: 973 54 1620 4971 Fragments arranged by size: 4971 1620 973 54 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6229 136 Size: 107 122 71 631 605 4557 1525 Fragments arranged by size: 4557 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2326 2888 7613 2326 Size: 562 4725 2331 Fragments arranged by size: 4725 2331 562 BlpI GC'TnA_GC Cuts at: 4308 5035 4308 Size: 727 6891 Bme1580I G_kGCm'C Cuts at: 1790 4156 5679 6835 1790 Size: 2366 1523 1156 2573 Fragments arranged by size: 2573 2366 1523 1156 BmgBI CAC'GTC Cuts at: 1318 4697 1318 Size: 3379 4239 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3596 4678 4752 Size: 416 53 83 399 24 2179 1082 74 Cuts at: 4752 5349 6307 442 Size: 597 958 1753 Fragments arranged by size: 2179 1753 1082 958 597 416 399 83 74 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7618 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2242 3297 2242 Size: 1055 6563 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2971 3491 3549 3565 6278 1239 Size: 1732 520 58 16 2713 2579 Fragments arranged by size: 2713 2579 1732 520 58 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2624 3819 3828 5743 6608 6906 2624 Size: 1195 9 1915 865 298 3336 Fragments arranged by size: 3336 1915 1195 865 298 9 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2748 3261 6281 882 Size: 617 1249 513 3020 2219 Fragments arranged by size: 3020 2219 1249 617 513 BsaAI yAC'GTr Cuts at: 493 1204 2438 5409 493 Size: 711 1234 2971 2702 Fragments arranged by size: 2971 2702 1234 711 BsaBI GATnn'nnATC Cuts at: 7612 7612 Size: 7618 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2851 4656 5425 5807 Size: 53 83 186 936 1318 1805 769 382 Cuts at: 5807 6442 275 Size: 635 1451 Fragments arranged by size: 1805 1451 1318 936 769 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4665 6051 6449 6723 6870 7281 4665 Size: 1386 398 274 147 411 5002 Fragments arranged by size: 5002 1386 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3563 3593 4548 4578 4754 Size: 30 165 30 2160 30 955 30 176 Cuts at: 4754 4784 1178 Size: 30 4012 Fragments arranged by size: 4012 2160 955 176 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3390 3414 3417 3420 1186 Size: 2204 24 3 3 5384 Fragments arranged by size: 5384 2204 24 3 3 BseYI C'CCAG_C Cuts at: 2841 4050 6821 7317 2841 Size: 1209 2771 496 3142 Fragments arranged by size: 3142 2771 1209 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4439 4797 1163 Size: 573 2703 358 3984 Fragments arranged by size: 3984 2703 573 358 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5068 5829 6857 1540 Size: 275 186 3067 761 1028 2301 Fragments arranged by size: 3067 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3507 5679 5764 6835 3507 Size: 2172 85 1071 4290 Fragments arranged by size: 4290 2172 1071 85 BsmI GAATG_Cn' Cuts at: 3930 4825 5129 5222 3930 Size: 895 304 93 6326 Fragments arranged by size: 6326 895 304 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3507 4156 4937 5679 5764 6835 1391 Size: 399 1717 649 781 742 85 1071 2174 Fragments arranged by size: 2174 1717 1071 781 742 649 399 85 BspEI T'CCGG_A Cuts at: 7281 7281 Size: 7618 BspHI T'CATG_A Cuts at: 3372 5507 7237 3372 Size: 2135 1730 3753 Fragments arranged by size: 3753 2135 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3001 3661 3999 4973 844 Size: 2157 660 338 974 3489 Fragments arranged by size: 3489 2157 974 660 338 BsrBI CCG'CTC Cuts at: 1394 1823 5505 1394 Size: 429 3682 3507 Fragments arranged by size: 3682 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6113 6287 65 Size: 1183 4865 174 1396 Fragments arranged by size: 4865 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6262 6449 1700 Size: 110 4452 187 2869 Fragments arranged by size: 4452 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4073 96 Size: 3977 3641 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7618 BssSI C'ACGA_G Cuts at: 5678 6690 5678 Size: 1012 6606 BstAPI GCAn_nnn'nTGC Cuts at: 794 4820 794 Size: 4026 3592 BstBI TT'CG_AA Cuts at: 2980 6422 2980 Size: 3442 4176 BstEII G'GTnAC_C Cuts at: 1184 3986 1184 Size: 2802 4816 BstXI CCAn_nnnn'nTGG Cuts at: 2949 3173 4765 2949 Size: 224 1592 5802 Fragments arranged by size: 5802 1592 224 BstYI r'GATC_y Cuts at: 1972 2187 2326 2888 3161 3905 4662 4948 5315 Size: 215 139 562 273 744 757 286 367 Cuts at: 5315 5700 5717 7169 7255 7267 7613 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 757 744 562 385 367 346 286 273 215 139 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7066 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6879 Fragments arranged by size: 6879 683 56 BtsI GCAGTG_nn' Cuts at: 3641 3827 4175 4237 4427 4726 4731 5134 5928 Size: 186 348 62 190 299 5 403 794 Cuts at: 5928 5948 3641 Size: 20 5311 Fragments arranged by size: 5311 794 403 348 299 190 186 62 20 5 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7618 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4528 4563 529 Size: 35 3964 35 3584 Fragments arranged by size: 3964 3584 35 35 DraI TTT'AAA Cuts at: 4648 4922 4648 Size: 274 7344 DraIII CAC_nnn'GTG Cuts at: 4100 4100 Size: 7618 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4244 6625 809 Size: 507 2928 2381 1802 Fragments arranged by size: 2928 2381 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3751 4420 5065 Size: 54 1425 50 275 18 1921 669 645 Cuts at: 5065 5954 7583 8 Size: 889 1629 43 Fragments arranged by size: 1921 1629 1425 889 669 645 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5065 1537 Size: 275 3253 4090 Fragments arranged by size: 4090 3253 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2211 2730 2982 3982 5548 1353 Size: 858 519 252 1000 1566 3423 Fragments arranged by size: 3423 1566 1000 858 519 252 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6205 6579 6725 387 Size: 1064 138 4616 374 146 1280 Fragments arranged by size: 4616 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2865 2971 3001 3329 3491 3549 3565 Size: 393 1233 106 30 328 162 58 16 Cuts at: 3565 3789 3963 4847 5681 6278 7065 7333 1239 Size: 224 174 884 834 597 787 268 1524 Fragments arranged by size: 1524 1233 884 834 787 597 393 328 268 224 174 162 106 58 30 16 EcoO109I rG'GnC_Cy Cuts at: 4678 4678 Size: 7618 EcoRI G'AATT_C Cuts at: 3849 4927 5305 3849 Size: 1078 378 6162 Fragments arranged by size: 6162 1078 378 EcoRV GAT'ATC Cuts at: 3470 3470 Size: 7618 FspI TGC'GCA Cuts at: 3657 6124 3657 Size: 2467 5151 HaeII r_GCGC'y Cuts at: 1678 2854 4659 6765 1678 Size: 1176 1805 2106 2531 Fragments arranged by size: 2531 2106 1805 1176 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3655 3687 3777 3809 4368 Size: 32 135 32 2083 32 90 32 559 Cuts at: 4368 4400 4670 4702 4754 4786 6259 6291 6333 Size: 32 270 32 52 32 1473 32 42 Cuts at: 6333 6365 1373 Size: 32 2626 Fragments arranged by size: 2626 2083 1473 559 270 135 90 52 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2196 3959 4849 4962 5208 669 Size: 988 281 258 1763 890 113 246 3079 Fragments arranged by size: 3079 1763 988 890 281 258 246 113 HindIII A'AGCT_T Cuts at: 748 4628 748 Size: 3880 3738 HpaI GTT'AAC Cuts at: 2196 4849 5208 2196 Size: 2653 359 4606 Fragments arranged by size: 4606 2653 359 KasI G'GCGC_C Cuts at: 2850 4655 2850 Size: 1805 5813 KpnI G_GTAC'C Cuts at: 4943 4943 Size: 7618 MfeI C'AATT_G Cuts at: 5217 5217 Size: 7618 MluI A'CGCG_T Cuts at: 7494 7494 Size: 7618 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3694 6732 6916 1305 Size: 2389 3038 184 2007 Fragments arranged by size: 3038 2389 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3753 4422 10 Size: 54 1425 343 1921 669 3206 Fragments arranged by size: 3206 1921 1425 669 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 5576 5935 6094 518 Size: 648 843 3567 359 159 2042 Fragments arranged by size: 3567 2042 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4079 4312 4811 5034 5711 6859 7104 1606 Size: 2473 233 499 223 677 1148 245 2120 Fragments arranged by size: 2473 2120 1148 677 499 245 233 223 NarI GG'CG_CC Cuts at: 2851 4656 2851 Size: 1805 5813 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7066 NdeI CA'TA_TG Cuts at: 387 387 Size: 7618 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7618 NotI GC'GGCC_GC Cuts at: 5065 5065 Size: 7618 NspI r_CATG'y Cuts at: 1132 1229 4976 6521 1132 Size: 97 3747 1545 2229 Fragments arranged by size: 3747 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2367 1348 Size: 1019 6599 PciI A'CATG_T Cuts at: 6517 6517 Size: 7618 PflMI CCAn_nnn'nTGG Cuts at: 1108 4100 4418 1108 Size: 2992 318 4308 Fragments arranged by size: 4308 2992 318 PfoI T'CCnGG_A Cuts at: 4000 4674 4000 Size: 674 6944 PmeI GTTT'AAAC Cuts at: 4922 4922 Size: 7618 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5687 5719 7200 7232 5687 Size: 32 1481 32 6073 Fragments arranged by size: 6073 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4678 4678 Size: 7618 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7618 PsiI TTA'TAA Cuts at: 5188 5188 Size: 7618 PstI C_TGCA'G Cuts at: 830 4434 4970 830 Size: 3604 536 3478 Fragments arranged by size: 3604 3478 536 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7618 PvuII CAG'CTG Cuts at: 4079 4312 4811 4079 Size: 233 499 6886 Fragments arranged by size: 6886 499 233 SalI G'TCGA_C Cuts at: 1936 4960 1936 Size: 3024 4594 SanDI GG'GwC_CC Cuts at: 4678 4678 Size: 7618 SapI GCTCTTCn'nnn_ Cuts at: 3982 3982 Size: 7618 SbfI CC_TGCA'GG Cuts at: 4970 4970 Size: 7618 ScaI AGT'ACT Cuts at: 2933 5866 7578 2933 Size: 2933 1712 2973 Fragments arranged by size: 2973 2933 1712 SexAI A'CCwGG_T Cuts at: 2676 3952 4096 2676 Size: 1276 144 6198 Fragments arranged by size: 6198 1276 144 SfcI C'TryA_G Cuts at: 826 1045 2718 3937 4430 4966 6101 6782 6973 Size: 219 1673 1219 493 536 1135 681 191 Cuts at: 6973 826 Size: 1471 Fragments arranged by size: 1673 1471 1219 1135 681 536 493 219 191 SfoI GGC'GCC Cuts at: 2852 4657 2852 Size: 1805 5813 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7618 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7618 SmaI CCC'GGG Cuts at: 1872 4390 4945 1872 Size: 2518 555 4545 Fragments arranged by size: 4545 2518 555 SmlI C'TyrA_G Cuts at: 820 1017 1943 2639 3834 3843 5722 6623 6885 Size: 197 926 696 1195 9 1879 901 262 Cuts at: 6885 820 Size: 1553 Fragments arranged by size: 1879 1553 1195 926 901 696 262 197 9 SnaBI TAC'GTA Cuts at: 493 5409 493 Size: 4916 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7618 SphI G_CATG'C Cuts at: 1132 4976 1132 Size: 3844 3774 SspI AAT'ATT Cuts at: 5 52 2763 4187 4243 5542 5 Size: 47 2711 1424 56 1299 2081 Fragments arranged by size: 2711 2081 1424 1299 56 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7618 StyI C'CwwG_G Cuts at: 513 1065 3250 3703 4546 5057 513 Size: 552 2185 453 843 511 3074 Fragments arranged by size: 3074 2185 843 552 511 453 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2772 5645 5662 5815 6000 6445 325 Size: 1255 1192 2873 17 153 185 445 1498 Fragments arranged by size: 2873 1498 1255 1192 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2931 4015 4073 Size: 274 80 33 51 1443 954 1084 58 Cuts at: 4073 4434 5864 7576 96 Size: 361 1430 1712 138 Fragments arranged by size: 1712 1443 1430 1084 954 361 274 138 80 58 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3563 3595 7578 7610 3563 Size: 32 3983 32 3571 Fragments arranged by size: 3983 3571 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7618 XbaI T'CTAG_A Cuts at: 4373 4954 4373 Size: 581 7037 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3710 4076 3710 Size: 366 7252 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7618 XmaI C'CCGG_G Cuts at: 1870 4388 4943 1870 Size: 2518 555 4545 Fragments arranged by size: 4545 2518 555 XmnI GAAnn'nnTTC Cuts at: 2619 2764 4033 5747 2619 Size: 145 1269 1714 4490 Fragments arranged by size: 4490 1714 1269 145 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5426 276 Size: 53 83 186 936 3892 2468 Fragments arranged by size: 3892 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SfiI SrfI SwaI