Restriction map for
pFN21AE2154
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI BamHI BbrPI BbsI
BcgI BciVI BclI BfrI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsgI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstXI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
EcoT22I FbaI FseI HindIII HinfI
HpaI KpnI KspI MaeII MamI
MfeI MluI MseI MslI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfcI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tth111I
Van91I XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 980 CGCG CG^CG
AccIII 527 TCCGGA T^CCGGA
AcyI 268 GGCGCC G[GA]^CG[CT]C
AfaI 676 GTAC GT^AC
Alw44I 211 GTGCAC G^TGCAC
AocI 621 CCTGAGG CC^T[ACGT]AGG
ApaLI 211 GTGCAC G^TGCAC
AspHI 211 GTGCAC G[AT]GC[AT]^C
AviII 569 TGCGCA TGC^GCA
AvrII 846 CCTAGG C^CTAGG
BbeI 268 GGCGCC GGCGC^C
BglII 687 AGATCT A^GATCT
BlnI 846 CCTAGG C^CTAGG
BsaHI 268 GGCGCC G[GA]^CG[CT]C
BsaWI 527 TCCGGA [AT]^CCGG[AT]
BseAI 527 TCCGGA T^CCGGA
BsiHKAI 211 GTGCAC G[AT]GC[AT]^C
BsmI 41 GCATTC GAATGC(1/-1)
BspEI 527 TCCGGA T^CCGGA
BsrBI 138 CCGCTC CCGCTC(-3/-3)
BsrDI 302 GCAATG GCAATG(2/0)
BsrFI 757 GCCGGT [GA]^CCGG[CT]
BssSI 471 CTCGTG CACGAG(-5/-1)
BstAPI 754 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 105 GGTCACC G^GT[ACGT]ACC
BstPI 105 GGTCACC G^GT[ACGT]ACC
BstUI 980 CGCG CG^CG
Bsu36I 621 CCTGAGG CC^T[ACGT]AGG
Cfr10I 757 GCCGGT [GA]^CCGG[CT]
Eco81I 621 CCTGAGG CC^T[ACGT]AGG
EcoO65I 105 GGTCACC G^GT[ACGT]ACC
FauI 870 GCGGG CCCGC(4/6)
FspI 569 TGCGCA TGC^GCA
HaeII 268 GGCGCC [GA]GCGC^[CT]
HgaI 1014 GACGC GACGC(5/10)
Hin1I 268 GGCGCC G[GA]^CG[CT]C
HincII 167 GTCAAC GT[CT]^[GA]AC
HindII 167 GTCAAC GT[CT]^[GA]AC
KasI 268 GGCGCC G^GCGCC
MroI 527 TCCGGA T^CCGGA
MvnI 980 CGCG CG^CG
NarI 268 GGCGCC GG^CGCC
NcoI 147 CCATGG C^CATGG
PpuMI 458 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 676 GTAC GT^AC
ScaI 675 AGTACT AGT^ACT
SfoI 268 GGCGCC GGC^GCC
SmaI 541 CCCGGG CCC^GGG
StuI 410 AGGCCT AGG^CCT
TaqI 881 TCGA T^CGA
TthHB8I 881 TCGA T^CGA
XmaCI 541 CCCGGG C^CCGGG
XmaI 541 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 226 GAATTC [GA]^AATT[CT]
328 GAATTC
AlwI 363 GGATC GGATC(4/5)
902 GGATC
AlwNI 769 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
913 CAGAACCTG
ApoI 226 GAATTC [GA]^AATT[CT]
328 GAATTC
BanI 268 GGCGCC G^G[CT][GA]CC
760 GGTGCC
BanII 114 GAGCCC G[GA]GC[CT]^C
832 GGGCTC
BsaI 601 GAGACC GGTCTC(1/5)
468 GGTCTC
BseRI 259 CTCCTC GAGGAG(10/8)
691 CTCCTC
BsmAI 601 GAGAC GTCTC(1/5)
469 GTCTC
BsmFI 863 GGGAC GGGAC(10/14)
1035 GTCCC
BstF5I 842 CATCC GGATG(2/0)
873 GGATG
DraII 458 AGGTCCT [GA]G^G[ACGT]CC[CT]
770 AGGGCCT
DsaI 147 CCATGG C^C[GA][CT]GG
221 CCGTGG
EarI 403 CTCTTC CTCTTC(1/4)
721 CTCTTC
EcoNI 258 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
402 CCTCTTCCAGG
EcoO109I 458 AGGTCCT [GA]G^G[ACGT]CC[CT]
770 AGGGCCT
EcoRI 226 GAATTC G^AATTC
328 GAATTC
FokI 842 CATCC GGATG(9/13)
873 GGATG
HphI 107 TCACC GGTGA(8/7)
560 TCACC
Ksp632I 403 CTCTTC CTCTTC(1/4)
721 CTCTTC
MaeIII 106 GTCAC ^GT[ACGT]AC
908 GTGAC
NspI 788 ACATGC [GA]CATG^[CT]
866 ACATGC
SfaNI 501 GCATC GCATC(5/9)
841 GCATC
Tsp45I 106 GTCAC ^GT[GC]AC
908 GTGAC
XbaI 58 TCTAGA T^CTAGA
654 TCTAGA
XcmI 299 CCAGCAATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
803 CCAATAACTCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AluI AvaI AvaII BalI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaJI BsiYI BslI
BsoBI Bsp1286I BspMI BsrI BssKI
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoRII EcoT14I
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MaeI MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PvuII Sau3AI Sau96I
ScrFI StyI TseI Tsp509I TspRI
XhoII