Restriction map for
pFN21AE2620
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# NO CUT ENZYMES ( 114 enzymes) #
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Acc65I AccI AclI AcsI AflII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbvCI BciVI BfaI BfrI
BlnI BsaBI BsiWI BsmBI BsmI
BspDI BspHI BspLU11I BsrBI BsrDI
BssSI Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI DrdI Eam1105I
EarI Eco47III Eco81I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
HincII HindII HindIII HpaI KasI
KpnI Ksp632I MaeI MamI MfeI
MluI MluNI MscI MseI MunI
NarI NcoI NdeI NheI NruI
NsiI NspV PacI PflFI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SnaBI SpeI Sse8387I
SspI StyI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 622 GACGTC GACGT^C
AccIII 1265 TCCGGA T^CCGGA
AflIII 203 ACGTGT A^C[GA][CT]GT
BanI 1420 GGCACC G^G[CT][GA]CC
BbrPI 475 CACGTG CAC^GTG
BbsI 766 GAAGAC GAAGAC(2/6)
BcgI 1295 GCAGCGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1589 AGATCT A^GATCT
BpuAI 766 GAAGAC GAAGAC(2/6)
BsaAI 475 CACGTG [CT]AC^GT[GA]
BsaWI 1265 TCCGGA [AT]^CCGG[AT]
BseAI 1265 TCCGGA T^CCGGA
BspEI 1265 TCCGGA T^CCGGA
BsrI 1457 ACTGG ACTGG(1/-1)
BssHII 793 GCGCGC G^CGCGC
BstAPI 738 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 723 GGTCACC G^GT[ACGT]ACC
BstPI 723 GGTCACC G^GT[ACGT]ACC
BstXI 1424 CCAAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1589 AGATCT [GA]^GATC[CT]
DraIII 457 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 723 GGTCACC G^GT[ACGT]ACC
MflI 1589 AGATCT [GA]^GATC[CT]
MroI 1265 TCCGGA T^CCGGA
NotI 629 GCGGCCGC GC^GGCCGC
NspI 1036 GCATGC [GA]CATG^[CT]
PaeR7I 1375 CTCGAG C^TCGAG
PflMI 116 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 475 CACGTG CAC^GTG
PmlI 475 CACGTG CAC^GTG
PstI 1141 CTGCAG CTGCA^G
SgrAI 1350 CGCCGGCG C[GA]^CCGG[CT]G
SphI 1036 GCATGC GCATG^C
TaqI 1376 TCGA T^CGA
TthHB8I 1376 TCGA T^CGA
Van91I 116 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 116 CCAACCTGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1375 CTCGAG C^TCGAG
XhoII 1589 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
ApaI 921 GGGCCC GGGCC^C
1095 GGGCCC
AvaII 165 GGACC G^G[AT]CC
840 GGTCC
BclI 686 TGATCA T^GATCA
699 TGATCA
BsaI 514 GAGACC GGTCTC(1/5)
1024 GAGACC
BsmAI 514 GAGAC GTCTC(1/5)
1024 GAGAC
Bsp1407I 563 TGTACA T^GTACA
779 TGTACA
BsrGI 563 TGTACA T^GTACA
779 TGTACA
EagI 630 CGGCCG C^GGCCG
887 CGGCCG
EclXI 630 CGGCCG C^GGCCG
887 CGGCCG
Eco52I 630 CGGCCG C^GGCCG
887 CGGCCG
FbaI 686 TGATCA T^GATCA
699 TGATCA
HaeII 1278 GGCGCT [GA]GCGC^[CT]
1371 GGCGCT
HinfI 666 GAGTC G^A[ACGT]TC
1406 GACTC
KspI 1014 CCGCGG CCGC^GG
1056 CCGCGG
MboII 766 GAAGA GAAGA(8/7)
1587 GAAGA
MslI 198 CATCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1323 CACGGAGGTG
PleI 1406 GACTC GAGTC(4/5)
666 GAGTC
SacII 1014 CCGCGG CCGC^GG
1056 CCGCGG
SfcI 1128 CTACAG C^T[GA][CT]AG
1141 CTGCAG
SmaI 87 CCCGGG CCC^GGG
501 CCCGGG
SspBI 563 TGTACA T^GTACA
779 TGTACA
XmaCI 87 CCCGGG C^CCGGG
501 CCCGGG
XmaI 87 CCCGGG C^CCGGG
501 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI BanII BbvI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrFI BssKI BstF5I
BstNI BstUI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI MaeII MaeIII MboI MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StuI TseI
Tsp45I TspRI