Restriction map for
pFN21AE2703
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BfrI BglI BlnI BsaAI
BsaBI BseAI BseRI BsiEI BsiWI
BsmBI BsmFI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoT22I FseI FspI HaeII HhaI
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PvuI RcaI RsrII SacI
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 307 GTCTAC GT^[AC][GT]AC
AccII 203 CGCG CG^CG
AcyI 1438 GGCGTC G[GA]^CG[CT]C
AflIII 2477 ACGTGT A^C[GA][CT]GT
ApaI 451 GGGCCC GGGCC^C
AseI 1759 ATTAAT AT^TAAT
AsnI 1759 ATTAAT AT^TAAT
AspHI 2076 GAGCAC G[AT]GC[AT]^C
AvaI 1412 CCCGAG C^[CT]CG[GA]G
BalI 2312 TGGCCA TGG^CCA
BanII 451 GGGCCC G[GA]GC[CT]^C
BciVI 1584 GGATAC GTATCC(6/5)
BclI 1493 TGATCA T^GATCA
BglII 684 AGATCT A^GATCT
BlpI 858 GCTTAGC GC^T[ACGT]AGC
BpmI 623 CTCCAG CTGGAG(16/14)
Bpu1102I 858 GCTTAGC GC^T[ACGT]AGC
BsaHI 1438 GGCGTC G[GA]^CG[CT]C
BsaWI 1432 ACCGGA [AT]^CCGG[AT]
BsgI 1832 GTGCAG GTGCAG(16/14)
BsiHKAI 2076 GAGCAC G[AT]GC[AT]^C
BsoBI 1412 CCCGAG C^[CT]CG[GA]G
BsrBI 2318 CCGCTC CCGCTC(-3/-3)
BssSI 1557 CACGAG CACGAG(-5/-1)
BstUI 203 CGCG CG^CG
BstXI 138 CCATAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 684 AGATCT [GA]^GATC[CT]
CelII 858 GCTTAGC GC^T[ACGT]AGC
DraII 451 GGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 2312 TGGCCA [CT]^GGCC[GA]
EcoNI 455 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 451 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1174 GATATC GAT^ATC
FbaI 1493 TGATCA T^GATCA
Hin1I 1438 GGCGTC G[GA]^CG[CT]C
MflI 684 AGATCT [GA]^GATC[CT]
MluNI 2312 TGGCCA TGG^CCA
MscI 2312 TGGCCA TGG^CCA
MspA1I 973 CAGCTG C[AC]G^C[GT]G
MvnI 203 CGCG CG^CG
NcoI 773 CCATGG C^CATGG
NspI 1395 GCATGC [GA]CATG^[CT]
PshBI 1759 ATTAAT AT^TAAT
PvuII 973 CAGCTG CAG^CTG
SapI 831 GAAGAGC GCTCTTC(1/4)
ScaI 2450 AGTACT AGT^ACT
SexAI 542 ACCAGGT A^CC[AT]GGT
SpeI 171 ACTAGT A^CTAGT
SphI 1395 GCATGC GCATG^C
Tsp45I 1682 GTGAC ^GT[GC]AC
XhoII 684 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AclI 1030 AACGTT AA^CGTT
1039 AACGTT
AlwI 557 GGATC GGATC(4/5)
2267 GGATC
AlwNI 278 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
1680 CAGTGACTG
AocI 85 CCTAAGG CC^T[ACGT]AGG
459 CCTGAGG
Asp700I 1728 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2462 GAATGGATTC
AvaII 148 GGACC G^G[AT]CC
1068 GGTCC
BcnI 554 CCCGG CC^[GC]GG
1575 CCGGG
BmyI 451 GGGCCC G[AGT]GC[ACT]^C
2076 GAGCAC
BsmI 158 GCATTC GAATGC(1/-1)
1222 GCATTC
Bsp1286I 451 GGGCCC G[AGT]GC[ACT]^C
2076 GAGCAC
Bsu36I 85 CCTAAGG CC^T[ACGT]AGG
459 CCTGAGG
DsaI 773 CCATGG C^C[GA][CT]GG
1010 CCGTGG
Eco81I 85 CCTAAGG CC^T[ACGT]AGG
459 CCTGAGG
EcoRI 719 GAATTC G^AATTC
1158 GAATTC
EcoT14I 665 CCAAGG C^C[AT][AT]GG
773 CCATGG
FauI 1924 CCCGC CCCGC(4/6)
41 GCGGG
MslI 1295 CATACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2548 CATGTATATG
NciI 554 CCCGG CC^[GC]GG
1575 CCGGG
NdeI 248 CATATG CA^TATG
1952 CATATG
Psp1406I 1030 AACGTT AA^CGTT
1039 AACGTT
PstI 1723 CTGCAG CTGCA^G
2260 CTGCAG
StyI 665 CCAAGG C^C[AT][AT]GG
773 CCATGG
XbaI 1959 TCTAGA T^CTAGA
2322 TCTAGA
XcmI 797 CCAAAGAGGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2504 CCATGAAAAATATGG
XmnI 1728 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2462 GAATGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbsI BbvI BfaI BpuAI BsaI
BsaJI BsiYI BslI BsmAI BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I