Restriction map for
pFN21AE2632
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# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BbeI BbrPI BbvCI BglI BglII
BlnI BsaAI BsaBI BseAI BspDI
BspEI BssHII Bst1107I BstBI BstZ17I
ClaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRV FseI FspI
KasI KspI MamI MluI MroI
NarI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI PvuI SacI SacII
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AclI 2445 AACGTT AA^CGTT
AcyI 4432 GGCGTC G[GA]^CG[CT]C
AflII 783 CTTAAG C^TTAAG
AflIII 3985 ACATGT A^C[GA][CT]GT
AocI 4048 CCTGAGG CC^T[ACGT]AGG
AspHI 5022 GAGCAC G[AT]GC[AT]^C
BamHI 1081 GGATCC G^GATCC
BcgI 4559 GCAAGGAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 783 CTTAAG C^TTAAG
BlpI 3022 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3022 GCTGAGC GC^T[ACGT]AGC
BsaHI 4432 GGCGTC G[GA]^CG[CT]C
BsiEI 1030 CGACCG CG[GA][CT]^CG
BsiHKAI 5022 GAGCAC G[AT]GC[AT]^C
BsiWI 4830 CGTACG C^GTACG
BspHI 981 TCATGA T^CATGA
BspLU11I 3985 ACATGT A^CATGT
BsrBI 3025 GAGCGG CCGCTC(-3/-3)
BssSI 1352 CACGAG CACGAG(-5/-1)
BstEII 1109 GGTCACC G^GT[ACGT]ACC
BstPI 1109 GGTCACC G^GT[ACGT]ACC
Bsu36I 4048 CCTGAGG CC^T[ACGT]AGG
CelII 3022 GCTGAGC GC^T[ACGT]AGC
CpoI 504 CGGACCG CG^G[AT]CCG
DrdI 4426 GACTGTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4048 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1109 GGTCACC G^GT[ACGT]ACC
EcoRI 2254 GAATTC G^AATTC
EcoT22I 1091 ATGCAT ATGCA^T
Hin1I 4432 GGCGTC G[GA]^CG[CT]C
NaeI 3152 GCCGGC GCC^GGC
NgoMIV 3152 GCCGGC G^CCGGC
NheI 3043 GCTAGC G^CTAGC
NsiI 1091 ATGCAT ATGCA^T
Psp1406I 2445 AACGTT AA^CGTT
RcaI 981 TCATGA T^CATGA
RsrII 504 CGGACCG CG^G[AT]CCG
SalI 4435 GTCGAC G^TCGAC
SphI 5198 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 1444 GGTACC G^GTACC
2153 GGTACC
AccI 4435 GTCGAC GT^[AC][GT]AC
4987 GTAGAC
AccII 472 CGCG CG^CG
3760 CGCG
Asp700I 1089 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3310 GAAAATATTC
Asp718I 1444 GGTACC G^GTACC
2153 GGTACC
AvaI 1686 CCCGAG C^[CT]CG[GA]G
5353 CCCGAG
BalI 3742 TGGCCA TGG^CCA
4821 TGGCCA
BciVI 2521 GGATAC GTATCC(6/5)
558 GTATCC
BcnI 3144 CCCGG CC^[GC]GG
6151 CCCGG
BsaWI 1501 TCCGGT [AT]^CCGG[AT]
3799 ACCGGA
BsoBI 1686 CCCGAG C^[CT]CG[GA]G
5353 CCCGAG
Bsp1407I 3818 TGTACA T^GTACA
6140 TGTACA
BsrFI 2150 GCCGGT [GA]^CCGG[CT]
3152 GCCGGC
BsrGI 3818 TGTACA T^GTACA
6140 TGTACA
BstUI 472 CGCG CG^CG
3760 CGCG
BstXI 1755 CCACTTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5333 CCATGCAGCTGG
BstYI 1081 GGATCC [GA]^GATC[CT]
4140 AGATCC
Cfr10I 2150 GCCGGT [GA]^CCGG[CT]
3152 GCCGGC
DraI 1840 TTTAAA TTT^AAA
3805 TTTAAA
DraIII 1352 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4013 CACCAGGTG
HpaI 1279 GTTAAC GTT^AAC
5466 GTTAAC
KpnI 1444 GGTACC GGTAC^C
2153 GGTACC
MfeI 341 CAATTG C^AATTG
1847 CAATTG
MflI 1081 GGATCC [GA]^GATC[CT]
4140 AGATCC
MluNI 3742 TGGCCA TGG^CCA
4821 TGGCCA
MscI 3742 TGGCCA TGG^CCA
4821 TGGCCA
MunI 341 CAATTG C^AATTG
1847 CAATTG
MvnI 472 CGCG CG^CG
3760 CGCG
NciI 3144 CCCGG CC^[GC]GG
6151 CCCGG
NdeI 886 CATATG CA^TATG
6095 CATATG
PflMI 117 CCATGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5289 CCAGCAGCTGG
PshAI 1131 GACTAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4735 GACCAAAGTC
SspBI 3818 TGTACA T^GTACA
6140 TGTACA
Van91I 117 CCATGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5289 CCAGCAGCTGG
XbaI 283 TCTAGA T^CTAGA
2641 TCTAGA
XhoII 1081 GGATCC [GA]^GATC[CT]
4140 AGATCC
XmnI 1089 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3310 GAAAATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BanI BanII
BbsI BbvI BclI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmBI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScaI ScrFI SexAI SfaNI SfcI
SmlI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI