Restriction map for
pFN21AE3449
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# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AscI AseI AsnI Asp718I AviII
AvrII BbrPI BciVI BclI BfaI
BfrI BlnI BsaBI BsmI BspDI
BssHII Bst1107I BstZ17I ClaI CpoI
DraI EcoRI FbaI FseI FspI
HpaI KpnI MaeI MamI MfeI
MunI NdeI NheI NruI PacI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I RsrII SbfI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Van91I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 3777 TCCGGA T^CCGGA
BamHI 2497 GGATCC G^GATCC
BanI 3382 GGCGCC G^G[CT][GA]CC
BbeI 3382 GGCGCC GGCGC^C
BbvCI 3264 CCTCAGC CCTCAGC(-5/-2)
BsaAI 1804 TACGTG [CT]AC^GT[GA]
BsaI 1404 GGTCTC GGTCTC(1/5)
BsaWI 3777 TCCGGA [AT]^CCGG[AT]
BseAI 3777 TCCGGA T^CCGGA
BsiWI 3848 CGTACG C^GTACG
BspEI 3777 TCCGGA T^CCGGA
BsrDI 3600 CATTGC GCAATG(2/0)
BssSI 654 CACGAG CACGAG(-5/-1)
BstBI 806 TTCGAA TT^CGAA
BstEII 1216 GGTGACC G^GT[ACGT]ACC
BstPI 1216 GGTGACC G^GT[ACGT]ACC
DraIII 3715 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1216 GGTGACC G^GT[ACGT]ACC
EcoRV 733 GATATC GAT^ATC
EcoT22I 197 ATGCAT ATGCA^T
HincII 2641 GTCGAC GT[CT]^[GA]AC
HindII 2641 GTCGAC GT[CT]^[GA]AC
HindIII 271 AAGCTT A^AGCTT
KasI 3382 GGCGCC G^GCGCC
KspI 1592 CCGCGG CCGC^GG
MluI 2066 ACGCGT A^CGCGT
MroI 3777 TCCGGA T^CCGGA
NarI 3382 GGCGCC GG^CGCC
NcoI 2161 CCATGG C^CATGG
NotI 949 GCGGCCGC GC^GGCCGC
NsiI 197 ATGCAT ATGCA^T
NspV 806 TTCGAA TT^CGAA
PshAI 2359 GACGCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 716 CGATCG CGAT^CG
SacII 1592 CCGCGG CCGC^GG
SalI 2641 GTCGAC G^TCGAC
SapI 1152 GCTCTTC GCTCTTC(1/4)
ScaI 824 AGTACT AGT^ACT
SexAI 3767 ACCTGGT A^CC[AT]GGT
SfiI 2697 GGCCTGCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3382 GGCGCC GGC^GCC
SfuI 806 TTCGAA TT^CGAA
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AhdI 2230 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3889 GACGCCTGGTC
AocI 2752 CCTGAGG CC^T[ACGT]AGG
3397 CCTCAGG
Asp700I 447 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1986 GAAGATGTTC
AspEI 2230 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3889 GACGCCTGGTC
AspI 2635 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3222 GACCAAGTC
BalI 1645 TGGCCA TGG^CCA
1823 TGGCCA
BcgI 3828 CGACAAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3357 GCAGACAAGTCG
BglII 449 AGATCT A^GATCT
3543 AGATCT
BlpI 2730 GCTCAGC GC^T[ACGT]AGC
2787 GCTCAGC
Bpu1102I 2730 GCTCAGC GC^T[ACGT]AGC
2787 GCTCAGC
Bsp1407I 1454 TGTACA T^GTACA
1607 TGTACA
BspHI 263 TCATGA T^CATGA
1742 TCATGA
BspLU11I 1610 ACATGT A^CATGT
1673 ACATGT
BsrGI 1454 TGTACA T^GTACA
1607 TGTACA
BstXI 1104 CCAGCCGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1636 CCAGGCATCTGG
Bsu36I 2752 CCTGAGG CC^T[ACGT]AGG
3397 CCTCAGG
CelII 2730 GCTCAGC GC^T[ACGT]AGC
2787 GCTCAGC
DrdI 3072 GACAGCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3126 GACTGCAGCGTC
Eam1105I 2230 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3889 GACGCCTGGTC
Eco81I 2752 CCTGAGG CC^T[ACGT]AGG
3397 CCTCAGG
EcoNI 636 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2454 CCTGATGGAGG
MluNI 1645 TGGCCA TGG^CCA
1823 TGGCCA
MscI 1645 TGGCCA TGG^CCA
1823 TGGCCA
NaeI 1958 GCCGGC GCC^GGC
3192 GCCGGC
NgoMIV 1958 GCCGGC G^CCGGC
3192 GCCGGC
PaeR7I 416 CTCGAG C^TCGAG
2172 CTCGAG
PflFI 2635 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3222 GACCAAGTC
RcaI 263 TCATGA T^CATGA
1742 TCATGA
SacI 370 GAGCTC GAGCT^C
988 GAGCTC
SspBI 1454 TGTACA T^GTACA
1607 TGTACA
Tth111I 2635 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3222 GACCAAGTC
XhoI 416 CTCGAG C^TCGAG
2172 CTCGAG
XmnI 447 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1986 GAAGATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI Aor51HI ApaI ApaLI ApoI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco47III Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII