Restriction map for
pFN21AE2775
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I ApaLI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BalI BbvCI BcgI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BsrBI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraIII EarI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MamI MfeI MluI MluNI MroI
MscI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 1279 CGCG CG^CG
AflIII 524 ACATGT A^C[GA][CT]GT
AhdI 1446 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1690 CCTCAGG CC^T[ACGT]AGG
Aor51HI 27 AGCGCT AGC^GCT
ApaI 1548 GGGCCC GGGCC^C
AspEI 1446 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1161 GGATCC G^GATCC
BbeI 436 GGCGCC GGCGC^C
BbrPI 774 CACGTG CAC^GTG
BbsI 958 GAAGAC GAAGAC(2/6)
BciVI 1736 GTATCC GTATCC(6/5)
BfaI 1267 CTAG C^TAG
BglI 928 GCCTGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 958 GAAGAC GAAGAC(2/6)
BsiEI 17 CGGCCG CG[GA][CT]^CG
BsmI 1600 GAATGC GAATGC(1/-1)
BspLU11I 524 ACATGT A^CATGT
BsrDI 270 CATTGC GCAATG(2/0)
BstUI 1279 CGCG CG^CG
BstYI 1161 GGATCC [GA]^GATC[CT]
Bsu36I 1690 CCTCAGG CC^T[ACGT]AGG
DraI 1033 TTTAAA TTT^AAA
DrdI 739 GACTGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 669 CCGTGG C^C[GA][CT]GG
EagI 17 CGGCCG C^GGCCG
Eam1105I 1446 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 17 CGGCCG C^GGCCG
Eco47III 27 AGCGCT AGC^GCT
Eco52I 17 CGGCCG C^GGCCG
Eco81I 1690 CCTCAGG CC^T[ACGT]AGG
EcoRI 293 GAATTC G^AATTC
FauI 492 GCGGG CCCGC(4/6)
KasI 436 GGCGCC G^GCGCC
MaeI 1267 CTAG C^TAG
MflI 1161 GGATCC [GA]^GATC[CT]
MvnI 1279 CGCG CG^CG
NaeI 1837 GCCGGC GCC^GGC
NarI 436 GGCGCC GG^CGCC
NgoMIV 1837 GCCGGC G^CCGGC
NspI 524 ACATGT [GA]CATG^[CT]
PflMI 246 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 774 CACGTG CAC^GTG
PmlI 774 CACGTG CAC^GTG
PpuMI 1444 GGGACCT [GA]G^G[AT]CC[CT]
SfoI 436 GGCGCC GGC^GCC
SmaI 728 CCCGGG CCC^GGG
SmlI 1357 CTCAAG C^T[CT][GA]AG
SspI 1063 AATATT AAT^ATT
TaqI 529 TCGA T^CGA
TfiI 1218 GATTC G^A[AT]TC
TthHB8I 529 TCGA T^CGA
Van91I 246 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1161 GGATCC [GA]^GATC[CT]
XmaCI 728 CCCGGG C^CCGGG
XmaI 728 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AspHI 654 GTGCTC G[AT]GC[AT]^C
1527 GTGCTC
BanI 436 GGCGCC G^G[CT][GA]CC
862 GGTGCC
BsaAI 774 CACGTG [CT]AC^GT[GA]
819 CACGTA
BsaWI 388 ACCGGA [AT]^CCGG[AT]
1450 TCCGGT
BsiHKAI 654 GTGCTC G[AT]GC[AT]^C
1527 GTGCTC
BsmAI 764 GAGAC GTCTC(1/5)
1570 GTCTC
BsmBI 1569 CGTCTC CGTCTC(1/5)
764 GAGACG
BsrFI 1116 GCCGGT [GA]^CCGG[CT]
1837 GCCGGC
BssSI 99 CACGAG CACGAG(-5/-1)
915 CTCGTG
BstAPI 1595 GCAGGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1664 GCATACAGTGC
Cfr10I 1116 GCCGGT [GA]^CCGG[CT]
1837 GCCGGC
EaeI 17 CGGCCG [CT]^GGCC[GA]
1839 CGGCCA
EcoNI 1620 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1686 CCTGCCTCAGG
MslI 525 CATGTCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
867 CACCTGCGTG
MspA1I 555 CAGCTG C[AC]G^C[GT]G
1491 CAGCTG
PstI 310 CTGCAG CTGCA^G
710 CTGCAG
PvuII 555 CAGCTG CAG^CTG
1491 CAGCTG
SfaNI 376 GATGC GCATC(5/9)
482 GCATC
SfcI 310 CTGCAG C^T[GA][CT]AG
710 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspI AvaI
AvaII BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflFI PleI RsaI Sau3AI Sau96I
ScrFI StyI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I XcmI