Restriction map for
pFN21AE2776
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BsrBI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraIII Eam1105I EarI Eco81I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TfiI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
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AflIII 524 ACATGT A^C[GA][CT]GT
Aor51HI 27 AGCGCT AGC^GCT
AspHI 654 GTGCTC G[AT]GC[AT]^C
AvaII 180 GGTCC G^G[AT]CC
BamHI 1161 GGATCC G^GATCC
BanII 68 GGGCTC G[GA]GC[CT]^C
BbeI 436 GGCGCC GGCGC^C
BbrPI 774 CACGTG CAC^GTG
BbsI 958 GAAGAC GAAGAC(2/6)
BglI 928 GCCTGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 958 GAAGAC GAAGAC(2/6)
BsaWI 388 ACCGGA [AT]^CCGG[AT]
BsiEI 17 CGGCCG CG[GA][CT]^CG
BsiHKAI 654 GTGCTC G[AT]GC[AT]^C
BsmAI 764 GAGAC GTCTC(1/5)
BsmBI 764 GAGACG CGTCTC(1/5)
BspLU11I 524 ACATGT A^CATGT
BsrDI 270 CATTGC GCAATG(2/0)
BsrFI 1116 GCCGGT [GA]^CCGG[CT]
BstYI 1161 GGATCC [GA]^GATC[CT]
Cfr10I 1116 GCCGGT [GA]^CCGG[CT]
DraI 1033 TTTAAA TTT^AAA
DrdI 739 GACTGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 669 CCGTGG C^C[GA][CT]GG
EaeI 17 CGGCCG [CT]^GGCC[GA]
EagI 17 CGGCCG C^GGCCG
EclXI 17 CGGCCG C^GGCCG
Eco47III 27 AGCGCT AGC^GCT
Eco52I 17 CGGCCG C^GGCCG
EcoRI 293 GAATTC G^AATTC
FauI 492 GCGGG CCCGC(4/6)
KasI 436 GGCGCC G^GCGCC
MflI 1161 GGATCC [GA]^GATC[CT]
MspA1I 555 CAGCTG C[AC]G^C[GT]G
NarI 436 GGCGCC GG^CGCC
NspI 524 ACATGT [GA]CATG^[CT]
PflMI 246 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 774 CACGTG CAC^GTG
PmlI 774 CACGTG CAC^GTG
PvuII 555 CAGCTG CAG^CTG
SfoI 436 GGCGCC GGC^GCC
SmaI 728 CCCGGG CCC^GGG
SspI 1063 AATATT AAT^ATT
TaqI 529 TCGA T^CGA
TthHB8I 529 TCGA T^CGA
Van91I 246 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1161 GGATCC [GA]^GATC[CT]
XmaCI 728 CCCGGG C^CCGGG
XmaI 728 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 436 GGCGCC G[GA]^CG[CT]C
964 GGCGTC
AfaI 333 GTAC GT^AC
822 GTAC
AspI 496 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
961 GACGGCGTC
AvaI 728 CCCGGG C^[CT]CG[GA]G
841 CCCGAG
BanI 436 GGCGCC G^G[CT][GA]CC
862 GGTGCC
BpmI 151 CTCCAG CTGGAG(16/14)
73 CTGGAG
BsaAI 774 CACGTG [CT]AC^GT[GA]
819 CACGTA
BsaHI 436 GGCGCC G[GA]^CG[CT]C
964 GGCGTC
BsoBI 728 CCCGGG C^[CT]CG[GA]G
841 CCCGAG
BssSI 99 CACGAG CACGAG(-5/-1)
915 CTCGTG
Cac8I 42 GCTGGC GC[ACGT]^[ACGT]GC
712 GCAGGC
DraII 723 AGGCCCC [GA]G^G[ACGT]CC[CT]
990 AGGGCCT
EcoO109I 723 AGGCCCC [GA]G^G[ACGT]CC[CT]
990 AGGGCCT
EcoT14I 93 CCAAGG C^C[AT][AT]GG
183 CCTTGG
HgaI 920 GCGTC GACGC(5/10)
965 GCGTC
Hin1I 436 GGCGCC G[GA]^CG[CT]C
964 GGCGTC
MslI 525 CATGTCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
867 CACCTGCGTG
PflFI 496 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
961 GACGGCGTC
PstI 310 CTGCAG CTGCA^G
710 CTGCAG
RsaI 333 GTAC GT^AC
822 GTAC
SfaNI 376 GATGC GCATC(5/9)
482 GCATC
SfcI 310 CTGCAG C^T[GA][CT]AG
710 CTGCAG
StyI 93 CCAAGG C^C[AT][AT]GG
183 CCTTGG
Tth111I 496 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
961 GACGGCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI BbvI BcnI BmyI BsaJI
BseRI BsgI BsiYI BslI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI CfoI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI