Restriction map for
pFN21AE2748
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# NO CUT ENZYMES ( 70 enzymes) #
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AatII Acc65I AccII AgeI AhdI
AocI Aor51HI AscI Asp718I AspEI
AvrII BbeI BbrPI BcgI BciVI
BcnI BglI BlnI BsiWI BspDI
BsrFI BssHII BstEII BstPI BstUI
Bsu36I Cfr10I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I FseI KasI KpnI KspI
MluI MvnI NaeI NarI NciI
NgoMIV NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PvuI
RsrII SacI SacII SalI SbfI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 4340 TCCGGA T^CCGGA
AclI 3050 AACGTT AA^CGTT
AcyI 5763 GGCGTC G[GA]^CG[CT]C
Alw44I 6239 GTGCAC G^TGCAC
ApaLI 6239 GTGCAC G^TGCAC
AspI 2460 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3330 CTCGGG C^[CT]CG[GA]G
AviII 7169 TGCGCA TGC^GCA
BanI 6636 GGTGCC G^G[CT][GA]CC
BsaHI 5763 GGCGTC G[GA]^CG[CT]C
BsaI 5894 GAGACC GGTCTC(1/5)
BseAI 4340 TCCGGA T^CCGGA
BsiEI 787 CGACCG CG[GA][CT]^CG
BsmBI 3574 GAGACG CGTCTC(1/5)
BsoBI 3330 CTCGGG C^[CT]CG[GA]G
BspEI 4340 TCCGGA T^CCGGA
BsrBI 6175 GAGCGG CCGCTC(-3/-3)
Bst1107I 7025 GTATAC GTA^TAC
BstBI 1285 TTCGAA TT^CGAA
BstZ17I 7025 GTATAC GTA^TAC
DraIII 5270 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3782 GACAAAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 5732 GAATTC G^AATTC
EcoRV 111 GATATC GAT^ATC
FauI 6177 GCGGG CCCGC(4/6)
FspI 7169 TGCGCA TGC^GCA
HaeII 246 GGCGCT [GA]GCGC^[CT]
HgaI 5764 GCGTC GACGC(5/10)
Hin1I 5763 GGCGTC G[GA]^CG[CT]C
HincII 2751 GTTAAC GT[CT]^[GA]AC
HindII 2751 GTTAAC GT[CT]^[GA]AC
HpaI 2751 GTTAAC GTT^AAC
MroI 4340 TCCGGA T^CCGGA
NheI 1648 GCTAGC G^CTAGC
NspV 1285 TTCGAA TT^CGAA
PflFI 2460 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 5535 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3050 AACGTT AA^CGTT
SfuI 1285 TTCGAA TT^CGAA
SspI 6199 AATATT AAT^ATT
StuI 5119 AGGCCT AGG^CCT
Tth111I 2460 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2640 CCAGTTGGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 34 enzymes) #
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AflII 2520 CTTAAG C^TTAAG
4747 CTTAAG
AflIII 4101 ACATGT A^C[GA][CT]GT
4903 ACATGT
ApaI 5445 GGGCCC GGGCC^C
6805 GGGCCC
AseI 4693 ATTAAT AT^TAAT
5395 ATTAAT
AsnI 4693 ATTAAT AT^TAAT
5395 ATTAAT
BalI 2471 TGGCCA TGG^CCA
4268 TGGCCA
BamHI 5208 GGATCC G^GATCC
6348 GGATCC
BanII 5445 GGGCCC G[GA]GC[CT]^C
6805 GGGCCC
BbvCI 2659 CCTCAGC CCTCAGC(-5/-2)
2293 GCTGAGG
BclI 797 TGATCA T^GATCA
5300 TGATCA
BfrI 2520 CTTAAG C^TTAAG
4747 CTTAAG
BglII 303 AGATCT A^GATCT
2823 AGATCT
BsaAI 600 CACGTA [CT]AC^GT[GA]
7051 TACGTG
BspLU11I 4101 ACATGT A^CATGT
4903 ACATGT
BspMI 6060 ACCTGC ACCTGC(4/8)
4642 GCAGGT
BssSI 2972 CACGAG CACGAG(-5/-1)
6452 CTCGTG
BstXI 5744 CCAATTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6005 CCAAAATAATGG
CfoI 247 GCGC GCG^C
7170 GCGC
DsaI 2474 CCATGG C^C[GA][CT]GG
3182 CCATGG
EaeI 2471 TGGCCA [CT]^GGCC[GA]
4268 TGGCCA
FbaI 797 TGATCA T^GATCA
5300 TGATCA
HhaI 247 GCGC GCG^C
7170 GCGC
HinP1I 247 GCGC G^CGC
7170 GCGC
HindIII 628 AAGCTT A^AGCTT
6329 AAGCTT
MluNI 2471 TGGCCA TGG^CCA
4268 TGGCCA
MscI 2471 TGGCCA TGG^CCA
4268 TGGCCA
NcoI 2474 CCATGG C^CATGG
3182 CCATGG
NdeI 257 CATATG CA^TATG
5296 CATATG
PpuMI 906 AGGACCC [GA]G^G[AT]CC[CT]
1098 AGGACCT
PshBI 4693 ATTAAT AT^TAAT
5395 ATTAAT
SapI 10 GAAGAGC GCTCTTC(1/4)
2256 GCTCTTC
ScaI 479 AGTACT AGT^ACT
694 AGTACT
SpeI 2436 ACTAGT A^CTAGT
4216 ACTAGT
SphI 4887 GCATGC GCATG^C
5699 GCATGC
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# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BbsI BbvI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaBI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspHI BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CelII Cfr13I DdeI
DpnII DraI DraII EarI EcoNI
EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MfeI
MflI MnlI MseI MslI MspA1I
MspI MunI MvaI MwoI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XhoII
XmnI