Restriction map for
pFN21AE2711
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AclI AflII AflIII AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AvrII BamHI BbrPI BbvCI BclI
BfrI BglII BlnI BsgI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI Eam1105I Eco47III
Eco81I EcoRV EcoT22I FbaI FseI
HindIII HpaI KspI MfeI MluI
MunI NaeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SbfI ScaI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspBI
Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 63 enzymes) #
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Acc65I 2536 GGTACC G^GTACC
AccI 1354 GTCGAC GT^[AC][GT]AC
AccII 514 CGCG CG^CG
AccIII 1363 TCCGGA T^CCGGA
AcyI 2020 GGCGCC G[GA]^CG[CT]C
AgeI 1661 ACCGGT A^CCGGT
Asp700I 2703 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2536 GGTACC G^GTACC
AspI 1974 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1432 TGCGCA TGC^GCA
BbeI 2020 GGCGCC GGCGC^C
BcgI 2186 GCATTCGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 509 GTATCC GTATCC(6/5)
BglI 1141 GCCATGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1653 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1653 GCTGAGC GC^T[ACGT]AGC
BsaAI 2175 TACGTG [CT]AC^GT[GA]
BsaBI 201 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2020 GGCGCC G[GA]^CG[CT]C
BseAI 1363 TCCGGA T^CCGGA
BsmI 2186 GCATTC GAATGC(1/-1)
BspEI 1363 TCCGGA T^CCGGA
BspMI 1938 ACCTGC ACCTGC(4/8)
BsrFI 1661 ACCGGT [GA]^CCGG[CT]
BstAPI 1465 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 514 CGCG CG^CG
CelII 1653 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1661 ACCGGT [GA]^CCGG[CT]
DraI 718 TTTAAA TTT^AAA
DraIII 521 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1707 CGGCCG C^GGCCG
EclXI 1707 CGGCCG C^GGCCG
Eco52I 1707 CGGCCG C^GGCCG
EcoNI 84 CCTTACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1432 TGCGCA TGC^GCA
HaeII 2020 GGCGCC [GA]GCGC^[CT]
HgaI 2489 GCGTC GACGC(5/10)
Hin1I 2020 GGCGCC G[GA]^CG[CT]C
KasI 2020 GGCGCC G^GCGCC
KpnI 2536 GGTACC GGTAC^C
MamI 201 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1363 TCCGGA T^CCGGA
MvnI 514 CGCG CG^CG
NarI 2020 GGCGCC GG^CGCC
NdeI 168 CATATG CA^TATG
PflFI 1974 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1661 ACCGGT A^CCGGT
PpuMI 1585 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 2484 GACAAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1267 GAGCTC GAGCT^C
SalI 1354 GTCGAC G^TCGAC
SapI 951 GAAGAGC GCTCTTC(1/4)
SexAI 522 ACCAGGT A^CC[AT]GGT
SfoI 2020 GGCGCC GGC^GCC
SgrAI 1660 CACCGGTG C[GA]^CCGG[CT]G
SmaI 2576 CCCGGG CCC^GGG
SspI 76 AATATT AAT^ATT
StuI 2718 AGGCCT AGG^CCT
SwaI 717 ATTTAAAT ATTT^AAAT
Tth111I 1974 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2576 CCCGGG C^CCGGG
XmaI 2576 CCCGGG C^CCGGG
XmnI 2703 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwI 2526 GATCC GGATC(4/5)
2640 GGATC
AspHI 1097 GTGCTC G[AT]GC[AT]^C
1267 GAGCTC
BalI 468 TGGCCA TGG^CCA
782 TGGCCA
BanI 2020 GGCGCC G^G[CT][GA]CC
2536 GGTACC
BfaI 659 CTAG C^TAG
823 CTAG
BpmI 242 CTCCAG CTGGAG(16/14)
2521 CTGGAG
BsaI 259 GAGACC GGTCTC(1/5)
2580 GGTCTC
BsaWI 1363 TCCGGA [AT]^CCGG[AT]
1661 ACCGGT
BsiEI 1707 CGGCCG CG[GA][CT]^CG
2046 CGACCG
BsiHKAI 1097 GTGCTC G[AT]GC[AT]^C
1267 GAGCTC
BsmBI 1012 CGTCTC CGTCTC(1/5)
2692 GAGACG
BsrBI 239 CCGCTC CCGCTC(-3/-3)
1320 CCGCTC
BssSI 1375 CACGAG CACGAG(-5/-1)
1270 CTCGTG
BstXI 1169 CCAACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1289 CCAACCCTGTGG
BstYI 2525 AGATCC [GA]^GATC[CT]
2640 GGATCT
DrdI 900 GACTGCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1753 GACATGGGTGTC
EarI 34 CTCTTC CTCTTC(1/4)
951 GAAGAG
EcoRI 1006 GAATTC G^AATTC
1342 GAATTC
Ksp632I 34 CTCTTC CTCTTC(1/4)
951 GAAGAG
MaeI 659 CTAG C^TAG
823 CTAG
MaeII 2176 ACGT A^CGT
2246 ACGT
MflI 2525 AGATCC [GA]^GATC[CT]
2640 GGATCT
MluNI 468 TGGCCA TGG^CCA
782 TGGCCA
MscI 468 TGGCCA TGG^CCA
782 TGGCCA
NcoI 1175 CCATGG C^CATGG
1326 CCATGG
SmlI 973 CTTGAG C^T[CT][GA]AG
1581 CTCAAG
XhoII 2525 AGATCC [GA]^GATC[CT]
2640 GGATCT
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaI AvaII BanII BbsI BbvI
BcnI BmyI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstEII BstF5I
BstNI BstPI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI