Restriction map for
pFN21AE5738
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BanII BbeI BcgI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaHI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspLU11I BspMI BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoRI EcoRV
FauI FbaI FseI FspI HaeII
Hin1I HindIII HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PflFI PflMI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I Tth111I Van91I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AflIII 758 ACGTGT A^C[GA][CT]GT
AgeI 719 ACCGGT A^CCGGT
Asp700I 61 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1618 GAGCAC G[AT]GC[AT]^C
AvaI 572 CTCGAG C^[CT]CG[GA]G
AvaII 199 GGTCC G^G[AT]CC
BbsI 222 GAAGAC GAAGAC(2/6)
BciVI 1568 GTATCC GTATCC(6/5)
BcnI 1595 CCGGG CC^[GC]GG
BfaI 1027 CTAG C^TAG
BglII 1779 AGATCT A^GATCT
BmyI 1618 GAGCAC G[AGT]GC[ACT]^C
BpuAI 222 GAAGAC GAAGAC(2/6)
BsaBI 1563 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1943 GGTCTC GGTCTC(1/5)
BsaWI 719 ACCGGT [AT]^CCGG[AT]
BsgI 641 GTGCAG GTGCAG(16/14)
BsiHKAI 1618 GAGCAC G[AT]GC[AT]^C
BsmAI 1944 GTCTC GTCTC(1/5)
BsmFI 1457 GTCCC GGGAC(10/14)
BsoBI 572 CTCGAG C^[CT]CG[GA]G
Bsp1286I 1618 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 896 TGTACA T^GTACA
BspHI 432 TCATGA T^CATGA
BsrFI 719 ACCGGT [GA]^CCGG[CT]
BsrGI 896 TGTACA T^GTACA
BstEII 394 GGTGACC G^GT[ACGT]ACC
BstPI 394 GGTGACC G^GT[ACGT]ACC
CfoI 752 GCGC GCG^C
Cfr10I 719 ACCGGT [GA]^CCGG[CT]
Cfr13I 199 GGTCC G^G[ACGT]CC
DraI 1900 TTTAAA TTT^AAA
EcoNI 1034 CCTTATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 394 GGTGACC G^GT[ACGT]ACC
EcoT14I 1469 CCATGG C^C[AT][AT]GG
HgaI 277 GACGC GACGC(5/10)
HhaI 752 GCGC GCG^C
HinP1I 752 GCGC G^CGC
HincII 1657 GTTGAC GT[CT]^[GA]AC
HindII 1657 GTTGAC GT[CT]^[GA]AC
MaeI 1027 CTAG C^TAG
MamI 1563 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 1595 CCGGG CC^[GC]GG
NcoI 1469 CCATGG C^CATGG
NdeI 802 CATATG CA^TATG
NlaIV 1667 GGTTCC GG[ACGT]^[ACGT]CC
PaeR7I 572 CTCGAG C^TCGAG
PinAI 719 ACCGGT A^CCGGT
RcaI 432 TCATGA T^CATGA
SapI 266 GAAGAGC GCTCTTC(1/4)
Sau96I 199 GGTCC G^G[ACGT]CC
SmlI 572 CTCGAG C^T[CT][GA]AG
SspBI 896 TGTACA T^GTACA
StyI 1469 CCATGG C^C[AT][AT]GG
SwaI 1899 ATTTAAAT ATTT^AAAT
XhoI 572 CTCGAG C^TCGAG
XmnI 61 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BbrPI 757 CACGTG CAC^GTG
1621 CACGTG
BbvCI 1368 CCTCAGC CCTCAGC(-5/-2)
1056 GCTGAGG
BsaAI 757 CACGTG [CT]AC^GT[GA]
1621 CACGTG
BsmI 1552 GAATGC GAATGC(1/-1)
422 GCATTC
BstYI 466 GGATCT [GA]^GATC[CT]
1779 AGATCT
DsaI 767 CCACGG C^C[GA][CT]GG
1469 CCATGG
EcoT22I 1063 ATGCAT ATGCA^T
1217 ATGCAT
HapII 720 CCGG C^CGG
1595 CCGG
HpaII 720 CCGG C^CGG
1595 CCGG
MflI 466 GGATCT [GA]^GATC[CT]
1779 AGATCT
MspI 720 CCGG C^CGG
1595 CCGG
NsiI 1063 ATGCAT ATGCA^T
1217 ATGCAT
PmaCI 757 CACGTG CAC^GTG
1621 CACGTG
PmlI 757 CACGTG CAC^GTG
1621 CACGTG
SphI 409 GCATGC GCATG^C
1818 GCATGC
SspI 600 AATATT AAT^ATT
649 AATATT
StuI 1912 AGGCCT AGG^CCT
1949 AGGCCT
XcmI 300 CCAGGTGCATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2064 CCACGTTCCTCATGG
XhoII 466 GGATCT [GA]^GATC[CT]
1779 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BpmI BsaJI BseRI
BsiYI BslI BsrDI BsrI BssKI
BstF5I BstNI Cac8I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NspI PleI PvuII RsaI
Sau3AI ScrFI SfaNI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I