Restriction map for
pFN21AE2323
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# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AhdI Alw44I AocI ApaI ApaLI
AscI Asp718I AspEI AviII AvrII
BbeI BbrPI BcgI BglI BlnI
BseAI BsiWI BsmBI BspEI BspLU11I
BssHII BstBI BstEII BstPI Bsu36I
CpoI DraIII Eam1105I Eco81I EcoNI
EcoO65I FseI FspI HpaI KasI
KpnI MluI MroI NaeI NarI
NgoMIV NruI NspV PaeR7I PmaCI
PmeI PmlI PshAI Psp1406I RsrII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI Sse8387I StuI
SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 16 CGCG CG^CG
AcyI 3810 GACGCC G[GA]^CG[CT]C
AgeI 3149 ACCGGT A^CCGGT
Aor51HI 315 AGCGCT AGC^GCT
AspI 788 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 92 CCCGAG C^[CT]CG[GA]G
BamHI 923 GGATCC G^GATCC
BanI 3861 GGTGCC G^G[CT][GA]CC
BbvCI 1111 GCTGAGG CCTCAGC(-5/-2)
BclI 1248 TGATCA T^GATCA
BcnI 4287 CCCGG CC^[GC]GG
BglII 1872 AGATCT A^GATCT
BlpI 4751 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4751 GCTCAGC GC^T[ACGT]AGC
BsaAI 3644 TACGTA [CT]AC^GT[GA]
BsaHI 3810 GACGCC G[GA]^CG[CT]C
BsaI 2815 GGTCTC GGTCTC(1/5)
BsoBI 92 CCCGAG C^[CT]CG[GA]G
BspDI 646 ATCGAT AT^CGAT
BsrBI 21 CCGCTC CCGCTC(-3/-3)
BsrDI 561 CATTGC GCAATG(2/0)
BsrFI 3149 ACCGGT [GA]^CCGG[CT]
BssSI 3710 CACGAG CACGAG(-5/-1)
Bst1107I 4826 GTATAC GTA^TAC
BstAPI 556 GCATGCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 16 CGCG CG^CG
BstZ17I 4826 GTATAC GTA^TAC
CelII 4751 GCTCAGC GC^T[ACGT]AGC
CfoI 316 GCGC GCG^C
Cfr10I 3149 ACCGGT [GA]^CCGG[CT]
ClaI 646 ATCGAT AT^CGAT
DraI 1425 TTTAAA TTT^AAA
Eco47III 315 AGCGCT AGC^GCT
FauI 78 CCCGC CCCGC(4/6)
FbaI 1248 TGATCA T^GATCA
HaeII 315 AGCGCT [GA]GCGC^[CT]
HhaI 316 GCGC GCG^C
Hin1I 3810 GACGCC G[GA]^CG[CT]C
HinP1I 316 GCGC G^CGC
KspI 15 CCGCGG CCGC^GG
MvnI 16 CGCG CG^CG
NciI 4287 CCCGG CC^[GC]GG
NcoI 4232 CCATGG C^CATGG
NheI 4756 GCTAGC G^CTAGC
NotI 17 GCGGCCGC GC^GGCCGC
PacI 1585 TTAATTAA TTAAT^TAA
PflFI 788 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3149 ACCGGT A^CCGGT
PvuI 8 CGATCG CGAT^CG
SacII 15 CCGCGG CCGC^GG
SapI 3893 GAAGAGC GCTCTTC(1/4)
SnaBI 3644 TACGTA TAC^GTA
SphI 556 GCATGC GCATG^C
SspI 2698 AATATT AAT^ATT
Tth111I 788 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AlwNI 2168 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
5066 CAGAGGCTG
BciVI 1566 GGATAC GTATCC(6/5)
4926 GTATCC
BsaBI 1525 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3418 GATTTTAATC
BsaWI 2561 ACCGGA [AT]^CCGG[AT]
3149 ACCGGT
BseRI 67 GAGGAG GAGGAG(10/8)
1030 GAGGAG
BsgI 4986 CTGCAC GTGCAG(16/14)
1409 GTGCAG
BsmI 468 GAATGC GAATGC(1/-1)
4019 GAATGC
Bsp1407I 3456 TGTACA T^GTACA
5037 TGTACA
BspHI 4139 TCATGA T^CATGA
4893 TCATGA
BsrGI 3456 TGTACA T^GTACA
5037 TGTACA
BstXI 848 CCAGTAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2004 CCACCCTCATGG
DsaI 15 CCGCGG C^C[GA][CT]GG
4232 CCATGG
EagI 12 CGGCCG C^GGCCG
18 CGGCCG
EarI 1846 GAAGAG CTCTTC(1/4)
3893 GAAGAG
EclXI 12 CGGCCG C^GGCCG
18 CGGCCG
Eco52I 12 CGGCCG C^GGCCG
18 CGGCCG
EcoRI 2233 GAATTC G^AATTC
4479 GAATTC
HgaI 3810 GACGC GACGC(5/10)
2616 GCGTC
HindIII 193 AAGCTT A^AGCTT
4046 AAGCTT
Ksp632I 1846 GAAGAG CTCTTC(1/4)
3893 GAAGAG
MamI 1525 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3418 GATTTTAATC
MfeI 1502 CAATTG C^AATTG
2479 CAATTG
MunI 1502 CAATTG C^AATTG
2479 CAATTG
NspI 556 GCATGC [GA]CATG^[CT]
2329 GCATGT
PpuMI 1455 AGGACCT [GA]G^G[AT]CC[CT]
2896 GGGACCT
PstI 2165 CTGCAG CTGCA^G
2181 CTGCAG
PvuII 803 CAGCTG CAG^CTG
1109 CAGCTG
RcaI 4139 TCATGA T^CATGA
4893 TCATGA
SacI 3627 GAGCTC GAGCT^C
3696 GAGCTC
SpeI 2655 ACTAGT A^CTAGT
3169 ACTAGT
SspBI 3456 TGTACA T^GTACA
5037 TGTACA
XbaI 3091 TCTAGA T^CTAGA
3970 TCTAGA
XcmI 1766 CCAACCTGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4648 CCATATTCTGATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI ApoI AseI AsnI
Asp700I AspHI AvaII BalI BanII
BbsI BbvI BfaI BfrI BmyI
BpmI BpuAI BsaJI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DrdI EaeI EcoO109I EcoRII
EcoRV EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NdeI NdeII
NlaIII NlaIV NsiI PflMI PleI
PshBI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI