Restriction map for
pFN21AE4382
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# NO CUT ENZYMES ( 145 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AvaI AvaII
AviII AvrII BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI EagI
EarI EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRI EcoRV FseI FspI
HapII HincII HindII HpaI HpaII
KasI KpnI Ksp632I KspI MamI
MfeI MluI MroI MspI MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AatII 820 GACGTC GACGT^C
AciI 984 GCGG CCGC(-3/-1)
AcsI 1155 GAATTT [GA]^AATT[CT]
AhdI 214 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1023 CCTGAGG CC^T[ACGT]AGG
ApoI 1155 GAATTT [GA]^AATT[CT]
AspEI 214 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 921 GTGCTC G[AT]GC[AT]^C
AspI 382 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 156 TGGCCA TGG^CCA
BamHI 411 GGATCC G^GATCC
BclI 201 TGATCA T^GATCA
BfaI 241 CTAG C^TAG
BlpI 192 GCTCAGC GC^T[ACGT]AGC
BmyI 921 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 192 GCTCAGC GC^T[ACGT]AGC
BsaJI 260 CCATGG C^C[ACGT][ACGT]GG
BseRI 1026 GAGGAG GAGGAG(10/8)
BsgI 788 GTGCAG GTGCAG(16/14)
BsiHKAI 921 GTGCTC G[AT]GC[AT]^C
BsiYI 1194 CCTTTTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1194 CCTTTTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 578 GGGAC GGGAC(10/14)
Bsp1286I 921 GTGCTC G[AGT]GC[ACT]^C
BstXI 344 CCAAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 411 GGATCC [GA]^GATC[CT]
Bsu36I 1023 CCTGAGG CC^T[ACGT]AGG
CelII 192 GCTCAGC GC^T[ACGT]AGC
DsaI 260 CCATGG C^C[GA][CT]GG
EaeI 156 TGGCCA [CT]^GGCC[GA]
Eam1105I 214 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1023 CCTGAGG CC^T[ACGT]AGG
EcoNI 1194 CCTTTTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 260 CCATGG C^C[AT][AT]GG
EcoT22I 252 ATGCAT ATGCA^T
FauI 984 GCGGG CCCGC(4/6)
FbaI 201 TGATCA T^GATCA
HindIII 989 AAGCTT A^AGCTT
MaeI 241 CTAG C^TAG
MflI 411 GGATCC [GA]^GATC[CT]
MluNI 156 TGGCCA TGG^CCA
MscI 156 TGGCCA TGG^CCA
MspA1I 518 CAGCTG C[AC]G^C[GT]G
NcoI 260 CCATGG C^CATGG
NdeI 266 CATATG CA^TATG
NlaIV 411 GGATCC GG[ACGT]^[ACGT]CC
NsiI 252 ATGCAT ATGCA^T
PflFI 382 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 474 CTGCAG CTGCA^G
PvuII 518 CAGCTG CAG^CTG
SfcI 474 CTGCAG C^T[GA][CT]AG
SspI 316 AATATT AAT^ATT
StyI 260 CCATGG C^C[AT][AT]GG
TaqI 165 TCGA T^CGA
Tth111I 382 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 165 TCGA T^CGA
XcmI 837 CCAGCAGACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 411 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcyI 820 GACGTC G[GA]^CG[CT]C
997 GACGCC
AlwI 412 GATCC GGATC(4/5)
411 GGATC
BpmI 874 CTCCAG CTGGAG(16/14)
1136 CTGGAG
BsaHI 820 GACGTC G[GA]^CG[CT]C
997 GACGCC
BsaI 495 GGTCTC GGTCTC(1/5)
1017 GGTCTC
BsrDI 123 CATTGC GCAATG(2/0)
559 GCAATG
CfoI 228 GCGC GCG^C
595 GCGC
HaeII 227 AGCGCC [GA]GCGC^[CT]
594 GGCGCT
HgaI 997 GACGC GACGC(5/10)
803 GCGTC
HhaI 228 GCGC GCG^C
595 GCGC
Hin1I 820 GACGTC G[GA]^CG[CT]C
997 GACGCC
HinP1I 228 GCGC G^CGC
595 GCGC
MaeIII 222 GTCAC ^GT[ACGT]AC
1048 GTGAC
MseI 184 TTAA T^TAA
1150 TTAA
NspI 188 ACATGC [GA]CATG^[CT]
858 ACATGC
PleI 151 GAGTC GAGTC(4/5)
931 GAGTC
TfiI 485 GATTC G^A[AT]TC
1065 GATTC
Tru9I 184 TTAA T^TAA
1150 TTAA
Tsp45I 222 GTCAC ^GT[GC]AC
1048 GTGAC
Tsp509I 540 AATT ^AATT
1156 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AfaI AluI BbsI BbvI BpuAI
BsmAI BsrI BssKI BstF5I BstNI
Cac8I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeII MboI MboII MnlI MslI
MvaI MwoI NdeII NlaIII RsaI
Sau3AI ScrFI SfaNI TseI TspRI