Restriction map for
pFN21AE1980
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AlwNI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AviII
AvrII BanI BbeI BbrPI BcgI
BciVI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoT22I FseI FspI
HgaI Hin1I HpaI KasI KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I SspBI StuI
SwaI TaqI TthHB8I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AflII 892 CTTAAG C^TTAAG
AflIII 628 ACACGT A^C[GA][CT]GT
ApaI 1426 GGGCCC GGGCC^C
AspI 826 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 998 CTCGGG C^[CT]CG[GA]G
BalI 48 TGGCCA TGG^CCA
BamHI 600 GGATCC G^GATCC
BbvCI 397 GCTGAGG CCTCAGC(-5/-2)
BclI 1217 TGATCA T^GATCA
BfaI 283 CTAG C^TAG
BfrI 892 CTTAAG C^TTAAG
BsaAI 973 TACGTA [CT]AC^GT[GA]
BsaBI 595 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1055 GAGACC GGTCTC(1/5)
BseRI 1384 GAGGAG GAGGAG(10/8)
BsoBI 998 CTCGGG C^[CT]CG[GA]G
BspHI 119 TCATGA T^CATGA
BsrDI 641 GCAATG GCAATG(2/0)
CfoI 1551 GCGC GCG^C
DraI 622 TTTAAA TTT^AAA
DraIII 52 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 48 TGGCCA [CT]^GGCC[GA]
EcoNI 864 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1217 TGATCA T^GATCA
HaeII 1550 GGCGCT [GA]GCGC^[CT]
HhaI 1551 GCGC GCG^C
HinP1I 1551 GCGC G^CGC
HincII 1186 GTTGAC GT[CT]^[GA]AC
HindII 1186 GTTGAC GT[CT]^[GA]AC
MaeI 283 CTAG C^TAG
MamI 595 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 48 TGGCCA TGG^CCA
MscI 48 TGGCCA TGG^CCA
MslI 1365 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 674 CATATG CA^TATG
NheI 282 GCTAGC G^CTAGC
NspI 1319 GCATGT [GA]CATG^[CT]
PflFI 826 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 51 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 445 GAGTC GAGTC(4/5)
PpuMI 1194 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1196 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 416 CTGCAG CTGCA^G
RcaI 119 TCATGA T^CATGA
SfaNI 177 GATGC GCATC(5/9)
SmlI 892 CTTAAG C^T[CT][GA]AG
SnaBI 973 TACGTA TAC^GTA
SspI 949 AATATT AAT^ATT
TfiI 1270 GAATC G^A[AT]TC
Tth111I 826 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 51 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 721 GTAGAC GT^[AC][GT]AC
1203 GTCTAC
AcsI 451 AAATTC [GA]^AATT[CT]
619 GAATTT
ApoI 451 AAATTC [GA]^AATT[CT]
619 GAATTT
BanII 1174 GAGCCC G[GA]GC[CT]^C
1426 GGGCCC
BcnI 297 CCGGG CC^[GC]GG
700 CCGGG
BmyI 1174 GAGCCC G[AGT]GC[ACT]^C
1426 GGGCCC
BsmI 653 GAATGC GAATGC(1/-1)
1507 GCATTC
Bsp1286I 1174 GAGCCC G[AGT]GC[ACT]^C
1426 GGGCCC
BsrI 1512 CCAGT ACTGG(1/-1)
1590 CCAGT
BstXI 29 CCAACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
449 CCAAATTCTTGG
BstYI 600 GGATCC [GA]^GATC[CT]
851 AGATCC
DsaI 794 CCATGG C^C[GA][CT]GG
1178 CCATGG
EcoRV 751 GATATC GAT^ATC
1019 GATATC
FauI 1011 GCGGG CCCGC(4/6)
1107 GCGGG
HindIII 889 AAGCTT A^AGCTT
1490 AAGCTT
HinfI 445 GAGTC G^A[ACGT]TC
1270 GAATC
MflI 600 GGATCC [GA]^GATC[CT]
851 AGATCC
NciI 297 CCGGG CC^[GC]GG
700 CCGGG
NcoI 794 CCATGG C^CATGG
1178 CCATGG
SfcI 375 CTATAG C^T[GA][CT]AG
416 CTGCAG
XhoII 600 GGATCC [GA]^GATC[CT]
851 AGATCC
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI AocI
AvaII BbsI BbvI BpuAI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
BsrBI BssKI BstF5I BstNI Bsu36I
Cac8I Cfr13I DdeI DpnII DraII
EarI Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PvuII RsaI Sau3AI Sau96I
ScrFI StyI Tru9I TseI Tsp45I
Tsp509I TspRI