Restriction map for
pFN21AE4063
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BclI BfrI BglII BlnI
BsaAI BsaBI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstXI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
Eam1105I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MseI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 771 GACGTC GACGT^C
AlwNI 317 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 268 CCTCAGG CC^T[ACGT]AGG
Aor51HI 986 AGCGCT AGC^GCT
BalI 262 TGGCCA TGG^CCA
BanI 957 GGTGCC G^G[CT][GA]CC
BbsI 580 GTCTTC GAAGAC(2/6)
BciVI 447 GGATAC GTATCC(6/5)
BlpI 750 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 750 GCTGAGC GC^T[ACGT]AGC
BpuAI 580 GTCTTC GAAGAC(2/6)
BseRI 724 GAGGAG GAGGAG(10/8)
BspMI 903 ACCTGC ACCTGC(4/8)
BssSI 795 CACGAG CACGAG(-5/-1)
BstAPI 316 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 164 GGTTACC G^GT[ACGT]ACC
BstPI 164 GGTTACC G^GT[ACGT]ACC
BstYI 827 AGATCC [GA]^GATC[CT]
Bsu36I 268 CCTCAGG CC^T[ACGT]AGG
CelII 750 GCTGAGC GC^T[ACGT]AGC
EagI 776 CGGCCG C^GGCCG
EclXI 776 CGGCCG C^GGCCG
Eco47III 986 AGCGCT AGC^GCT
Eco52I 776 CGGCCG C^GGCCG
Eco81I 268 CCTCAGG CC^T[ACGT]AGG
EcoO65I 164 GGTTACC G^GT[ACGT]ACC
EcoT14I 299 CCTTGG C^C[AT][AT]GG
HaeII 986 AGCGCT [GA]GCGC^[CT]
MaeII 772 ACGT A^CGT
MflI 827 AGATCC [GA]^GATC[CT]
MluNI 262 TGGCCA TGG^CCA
MscI 262 TGGCCA TGG^CCA
PshAI 489 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 802 GAGCTC GAGCT^C
SapI 804 GCTCTTC GCTCTTC(1/4)
SexAI 953 ACCTGGT A^CC[AT]GGT
SfcI 245 CTGTAG C^T[GA][CT]AG
SmaI 707 CCCGGG CCC^GGG
StuI 272 AGGCCT AGG^CCT
StyI 299 CCTTGG C^C[AT][AT]GG
XhoII 827 AGATCC [GA]^GATC[CT]
XmaCI 707 CCCGGG C^CCGGG
XmaI 707 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 828 GATCC GGATC(4/5)
874 GATCC
AvaI 707 CCCGGG C^[CT]CG[GA]G
886 CCCGAG
BcgI 888 CGAGGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
758 GCAAGCACATCG
BsaI 622 GAGACC GGTCTC(1/5)
406 GGTCTC
BsaWI 155 ACCGGA [AT]^CCGG[AT]
432 ACCGGA
BsgI 784 CTGCAC GTGCAG(16/14)
905 CTGCAC
BsiEI 594 CGACCG CG[GA][CT]^CG
776 CGGCCG
BsmBI 459 GAGACG CGTCTC(1/5)
769 GAGACG
BsoBI 707 CCCGGG C^[CT]CG[GA]G
886 CCCGAG
BsrI 260 ACTGG ACTGG(1/-1)
720 ACTGG
DpnII 828 GATC ^GATC
874 GATC
EarI 805 CTCTTC CTCTTC(1/4)
194 GAAGAG
HinfI 295 GACTC G^A[ACGT]TC
489 GACTC
HphI 590 TCACC GGTGA(8/7)
923 TCACC
Ksp632I 805 CTCTTC CTCTTC(1/4)
194 GAAGAG
MboI 828 GATC ^GATC
874 GATC
NdeII 828 GATC ^GATC
874 GATC
PleI 295 GACTC GAGTC(4/5)
489 GACTC
Sau3AI 828 GATC ^GATC
874 GATC
TaqI 767 TCGA T^CGA
809 TCGA
TthHB8I 767 TCGA T^CGA
809 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AspHI AvaII BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrBI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
ItaI MaeI MaeIII MboII MnlI
MslI MspI MvaI MvnI MwoI
NciI NlaIII NlaIV Sau96I ScrFI
SfaNI TseI Tsp45I TspRI