Restriction map for
pFN21AE3221
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AocI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BclI BfrI BglII
BlnI BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI HphI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MseI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcsI 1108 AAATTC [GA]^AATT[CT]
AflIII 374 ACGTGT A^C[GA][CT]GT
AlwNI 466 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 452 AGCGCT AGC^GCT
ApaI 29 GGGCCC GGGCC^C
ApoI 1108 AAATTC [GA]^AATT[CT]
Asp700I 231 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 18 GGCGCC GGCGC^C
BciVI 330 GGATAC GTATCC(6/5)
BfaI 169 CTAG C^TAG
BsaAI 373 TACGTG [CT]AC^GT[GA]
BsaI 1252 GAGACC GGTCTC(1/5)
BsaWI 1442 ACCGGA [AT]^CCGG[AT]
BsiEI 1440 CGACCG CG[GA][CT]^CG
BsrDI 798 CATTGC GCAATG(2/0)
BssHII 1306 GCGCGC G^CGCGC
BstAPI 276 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 907 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 775 GACACCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 452 AGCGCT AGC^GCT
HgaI 1230 GACGC GACGC(5/10)
KasI 18 GGCGCC G^GCGCC
MaeI 169 CTAG C^TAG
MaeII 374 ACGT A^CGT
MslI 1266 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 18 GGCGCC GG^CGCC
PflMI 676 CCAGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1246 GACTC GAGTC(4/5)
SapI 915 GCTCTTC GCTCTTC(1/4)
ScaI 320 AGTACT AGT^ACT
SfiI 1049 GGCCCCGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 18 GGCGCC GGC^GCC
Van91I 676 CCAGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1160 CCACTCGACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 231 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 18 GGCGCC G[GA]^CG[CT]C
1230 GACGCC
AlwI 258 GATCC GGATC(4/5)
468 GATCC
BanI 18 GGCGCC G^G[CT][GA]CC
584 GGCACC
BbsI 1002 GAAGAC GAAGAC(2/6)
1339 GAAGAC
BglI 235 GCCTTCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1050 GCCCCGCAGGC
BlpI 480 GCTCAGC GC^T[ACGT]AGC
637 GCTGAGC
Bpu1102I 480 GCTCAGC GC^T[ACGT]AGC
637 GCTGAGC
BpuAI 1002 GAAGAC GAAGAC(2/6)
1339 GAAGAC
BsaHI 18 GGCGCC G[GA]^CG[CT]C
1230 GACGCC
BsrBI 43 GAGCGG CCGCTC(-3/-3)
49 GAGCGG
BstXI 762 CCAGACCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1007 CCAAGGCCATGG
BstYI 257 AGATCC [GA]^GATC[CT]
467 AGATCC
CelII 480 GCTCAGC GC^T[ACGT]AGC
637 GCTGAGC
EaeI 692 TGGCCG [CT]^GGCC[GA]
1454 TGGCCG
EarI 901 CTCTTC CTCTTC(1/4)
916 CTCTTC
EcoNI 344 CCTGCGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1428 CCTCCACGAGG
Hin1I 18 GGCGCC G[GA]^CG[CT]C
1230 GACGCC
Ksp632I 901 CTCTTC CTCTTC(1/4)
916 CTCTTC
MaeIII 784 GTCAC ^GT[ACGT]AC
824 GTGAC
MflI 257 AGATCC [GA]^GATC[CT]
467 AGATCC
NaeI 15 GCCGGC GCC^GGC
878 GCCGGC
NcoI 173 CCATGG C^CATGG
1013 CCATGG
NgoMIV 15 GCCGGC G^CCGGC
878 GCCGGC
NspI 143 ACATGC [GA]CATG^[CT]
833 ACATGC
PstI 498 CTGCAG CTGCA^G
1066 CTGCAG
PvuII 277 CAGCTG CAG^CTG
1357 CAGCTG
SacI 211 GAGCTC GAGCT^C
1408 GAGCTC
SfaNI 1272 GATGC GCATC(5/9)
1314 GCATC
SfcI 498 CTGCAG C^T[GA][CT]AG
1066 CTGCAG
SmlI 391 CTCAAG C^T[CT][GA]AG
1480 CTCAAG
StuI 233 AGGCCT AGG^CCT
341 AGGCCT
TfiI 590 GAATC G^A[AT]TC
1133 GAATC
Tsp45I 784 GTCAC ^GT[GC]AC
824 GTGAC
Tsp509I 398 AATT ^AATT
1109 AATT
XhoII 257 AGATCC [GA]^GATC[CT]
467 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
ApaLI AspHI AvaI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PpuMI RsaI Sau3AI Sau96I
ScrFI SmaI StyI TaqI TseI
TspRI TthHB8I XmaCI XmaI