Restriction map for
pFN21AE4362
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BglI BglII BlpI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BsiHKAI BsiWI BsmBI
BsmFI BsoBI BspEI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I CpoI DraI DraIII
DrdI DsaI Eam1105I Eco47III Eco81I
EcoNI EcoRI EcoT22I FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MfeI MluI MroI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AciI 866 CCGC CCGC(-3/-1)
AvrII 615 CCTAGG C^CTAGG
BalI 201 TGGCCA TGG^CCA
BanI 4 GGCACC G^G[CT][GA]CC
BbsI 388 GAAGAC GAAGAC(2/6)
BclI 372 TGATCA T^GATCA
BlnI 615 CCTAGG C^CTAGG
BmyI 3 GGGCAC G[AGT]GC[ACT]^C
BpuAI 388 GAAGAC GAAGAC(2/6)
BsaBI 478 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 934 GAGGAG GAGGAG(10/8)
BsgI 821 GTGCAG GTGCAG(16/14)
BsiEI 948 CGGCCG CG[GA][CT]^CG
BsmAI 749 GAGAC GTCTC(1/5)
BsmI 113 GCATTC GAATGC(1/-1)
Bsp1286I 3 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 859 TGTACA T^GTACA
BspDI 160 ATCGAT AT^CGAT
BspHI 102 TCATGA T^CATGA
BsrDI 123 CATTGC GCAATG(2/0)
BsrGI 859 TGTACA T^GTACA
BstXI 432 CCATTTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 829 GGATCT [GA]^GATC[CT]
ClaI 160 ATCGAT AT^CGAT
DraII 176 AGGGCCT [GA]G^G[ACGT]CC[CT]
EagI 948 CGGCCG C^GGCCG
EarI 73 GAAGAG CTCTTC(1/4)
EclXI 948 CGGCCG C^GGCCG
Eco52I 948 CGGCCG C^GGCCG
EcoO109I 176 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 478 GATATC GAT^ATC
FbaI 372 TGATCA T^GATCA
Ksp632I 73 GAAGAG CTCTTC(1/4)
MaeII 251 ACGT A^CGT
MamI 478 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 829 GGATCT [GA]^GATC[CT]
MluNI 201 TGGCCA TGG^CCA
MscI 201 TGGCCA TGG^CCA
MseI 23 TTAA T^TAA
PflMI 38 CCATCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 318 GAGTC GAGTC(4/5)
PshAI 246 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 212 CTGCAG CTGCA^G
RcaI 102 TCATGA T^CATGA
SexAI 258 ACCAGGT A^CC[AT]GGT
SfcI 212 CTGCAG C^T[GA][CT]AG
SspBI 859 TGTACA T^GTACA
Tru9I 23 TTAA T^TAA
Van91I 38 CCATCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 791 CCAAGGGAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 829 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 11 enzymes) #
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AlwI 829 GGATC GGATC(4/5)
877 GGATC
BstEII 582 GGTCACC G^GT[ACGT]ACC
1053 GGTGACC
BstPI 582 GGTCACC G^GT[ACGT]ACC
1053 GGTGACC
Cfr13I 177 GGGCC G^G[ACGT]CC
235 GGGCC
DdeI 316 CTGAG C^T[ACGT]AG
458 CTCAG
EaeI 201 TGGCCA [CT]^GGCC[GA]
948 CGGCCG
EcoO65I 582 GGTCACC G^GT[ACGT]ACC
1053 GGTGACC
MboII 73 GAAGA GAAGA(8/7)
388 GAAGA
Sau96I 177 GGGCC G^G[ACGT]CC
235 GGGCC
TaqI 161 TCGA T^CGA
595 TCGA
TthHB8I 161 TCGA T^CGA
595 TCGA
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BfaI BsaJI BsiYI BslI BspMI
BsrI BssKI BstF5I BstNI Cac8I
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeIII MboI MnlI MslI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI ScrFI SfaNI StyI TfiI
TseI Tsp45I Tsp509I TspRI