Restriction map for
pFN21AE2097
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspI AvaI AviII AvrII BalI
BanI BbeI BbrPI BcgI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoT14I EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI MaeII MamI MluI
MluNI MroI MscI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StyI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 655 CGCG CG^CG
AflIII 1361 ACATGT A^C[GA][CT]GT
AhdI 199 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1080 GTGCAC G^TGCAC
ApaLI 1080 GTGCAC G^TGCAC
AspEI 199 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1070 GGATCC G^GATCC
BanII 1765 GAGCCC G[GA]GC[CT]^C
BcnI 2120 CCCGG CC^[GC]GG
BglII 505 AGATCT A^GATCT
BsmAI 614 GAGAC GTCTC(1/5)
BspDI 2350 ATCGAT AT^CGAT
BspHI 1119 TCATGA T^CATGA
BspLU11I 1361 ACATGT A^CATGT
BsrDI 886 GCAATG GCAATG(2/0)
BstAPI 1150 GCATATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 655 CGCG CG^CG
ClaI 2350 ATCGAT AT^CGAT
DsaI 654 CCGCGG C^C[GA][CT]GG
EaeI 657 CGGCCA [CT]^GGCC[GA]
Eam1105I 199 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1800 GAATTC G^AATTC
EcoRV 2077 GATATC GAT^ATC
FauI 1498 CCCGC CCCGC(4/6)
HapII 2121 CCGG C^CGG
HindIII 280 AAGCTT A^AGCTT
HpaII 2121 CCGG C^CGG
KspI 654 CCGCGG CCGC^GG
MfeI 773 CAATTG C^AATTG
MspI 2121 CCGG C^CGG
MunI 773 CAATTG C^AATTG
MvnI 655 CGCG CG^CG
NciI 2120 CCCGG CC^[GC]GG
NspI 1361 ACATGT [GA]CATG^[CT]
PshAI 1825 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1119 TCATGA T^CATGA
SacII 654 CCGCGG CCGC^GG
SexAI 435 ACCAGGT A^CC[AT]GGT
SmlI 971 CTCAAG C^T[CT][GA]AG
StuI 899 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 1643 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1654 CAGAAACTG
Asp700I 712 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1656 GAAACTGTTC
BbvCI 525 GCTGAGG CCTCAGC(-5/-2)
1671 GCTGAGG
BciVI 2177 GGATAC GTATCC(6/5)
246 GTATCC
BpmI 501 CTGGAG CTGGAG(16/14)
1827 CTGGAG
BseRI 611 GAGGAG GAGGAG(10/8)
1999 GAGGAG
BsmI 418 GAATGC GAATGC(1/-1)
747 GAATGC
BstYI 505 AGATCT [GA]^GATC[CT]
1070 GGATCC
Cac8I 291 GCAGGC GC[ACGT]^[ACGT]GC
518 GCAAGC
EcoNI 725 CCTATGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1050 CCTGGGGAAGG
MflI 505 AGATCT [GA]^GATC[CT]
1070 GGATCC
PflMI 1037 CCACAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2185 CCAACACTTGG
PpuMI 1046 GGGACCT [GA]G^G[AT]CC[CT]
1552 AGGACCC
PstI 289 CTGCAG CTGCA^G
1232 CTGCAG
PvuII 286 CAGCTG CAG^CTG
523 CAGCTG
SapI 8 GCTCTTC GCTCTTC(1/4)
295 GCTCTTC
ScaI 382 AGTACT AGT^ACT
1958 AGTACT
SspI 157 AATATT AAT^ATT
918 AATATT
TaqI 1539 TCGA T^CGA
2351 TCGA
TthHB8I 1539 TCGA T^CGA
2351 TCGA
Van91I 1037 CCACAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2185 CCAACACTTGG
XcmI 74 CCAAGACATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1200 CCATGAAGCTGTTGG
XhoII 505 AGATCT [GA]^GATC[CT]
1070 GGATCC
XmnI 712 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1656 GAAACTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbsI BbvI
BfaI BmyI BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NdeI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI