Restriction map for
pFN21AE2737
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BbeI BbrPI BbsI
BcgI BciVI BclI BcnI BfrI
BlnI BpuAI BsaAI BsaBI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DrdI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HincII
HindII HpaI KasI KpnI MamI
MfeI MluI MroI MseI MslI
MunI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp45I Tsp509I Van91I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 361 GACGTC GACGT^C
AccI 817 GTCTAC GT^[AC][GT]AC
AspI 811 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 182 TGGCCA TGG^CCA
BamHI 104 GGATCC G^GATCC
BanI 391 GGCACC G^G[CT][GA]CC
BbvCI 21 CCTCAGC CCTCAGC(-5/-2)
BfaI 577 CTAG C^TAG
BglI 254 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 164 AGATCT A^GATCT
BlpI 600 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 600 GCTCAGC GC^T[ACGT]AGC
BsaI 406 GAGACC GGTCTC(1/5)
BsiEI 413 CGGCCG CG[GA][CT]^CG
BsmAI 406 GAGAC GTCTC(1/5)
BspHI 776 TCATGA T^CATGA
BsrFI 824 ACCGGC [GA]^CCGG[CT]
BsrI 479 ACTGG ACTGG(1/-1)
BssHII 689 GCGCGC G^CGCGC
BstAPI 522 GCACCGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 173 CCAAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 600 GCTCAGC GC^T[ACGT]AGC
Cfr10I 824 ACCGGC [GA]^CCGG[CT]
DraIII 523 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 413 CGGCCG C^GGCCG
EclXI 413 CGGCCG C^GGCCG
Eco52I 413 CGGCCG C^GGCCG
EcoNI 576 CCTAGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 203 CCATGG C^C[AT][AT]GG
HapII 825 CCGG C^CGG
HindIII 351 AAGCTT A^AGCTT
HpaII 825 CCGG C^CGG
KspI 410 CCGCGG CCGC^GG
MaeI 577 CTAG C^TAG
MaeIII 506 GTAAC ^GT[ACGT]AC
MluNI 182 TGGCCA TGG^CCA
MscI 182 TGGCCA TGG^CCA
MspI 825 CCGG C^CGG
NcoI 203 CCATGG C^CATGG
PaeR7I 763 CTCGAG C^TCGAG
PflFI 811 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 565 GACTC GAGTC(4/5)
RcaI 776 TCATGA T^CATGA
SacII 410 CCGCGG CCGC^GG
ScaI 35 AGTACT AGT^ACT
StyI 203 CCATGG C^C[AT][AT]GG
TspRI 636 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 811 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 763 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 361 GACGTC G[GA]^CG[CT]C
751 GGCGTC
AfaI 36 GTAC GT^AC
387 GTAC
AlwI 105 GATCC GGATC(4/5)
104 GGATC
ApaI 233 GGGCCC GGGCC^C
335 GGGCCC
AspHI 244 GAGCTC G[AT]GC[AT]^C
766 GAGCTC
BpmI 700 CTCCAG CTGGAG(16/14)
343 CTGGAG
BsaHI 361 GACGTC G[GA]^CG[CT]C
751 GGCGTC
BseRI 403 GAGGAG GAGGAG(10/8)
418 GAGGAG
BsiHKAI 244 GAGCTC G[AT]GC[AT]^C
766 GAGCTC
BsiYI 326 CCACTCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
576 CCTAGATAAGG
BslI 326 CCACTCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
576 CCTAGATAAGG
BstYI 104 GGATCC [GA]^GATC[CT]
164 AGATCT
DsaI 203 CCATGG C^C[GA][CT]GG
410 CCGCGG
EaeI 182 TGGCCA [CT]^GGCC[GA]
413 CGGCCG
EarI 43 CTCTTC CTCTTC(1/4)
58 CTCTTC
HgaI 780 GACGC GACGC(5/10)
752 GCGTC
Hin1I 361 GACGTC G[GA]^CG[CT]C
751 GGCGTC
Ksp632I 43 CTCTTC CTCTTC(1/4)
58 CTCTTC
MaeII 362 ACGT A^CGT
815 ACGT
MflI 104 GGATCC [GA]^GATC[CT]
164 AGATCT
NlaIII 204 CATG CATG^
777 CATG
PstI 160 CTGCAG CTGCA^G
659 CTGCAG
PvuII 340 CAGCTG CAG^CTG
445 CAGCTG
RsaI 36 GTAC GT^AC
387 GTAC
SacI 244 GAGCTC GAGCT^C
766 GAGCTC
SfcI 160 CTGCAG C^T[GA][CT]AG
659 CTGCAG
SmlI 238 CTCAAG C^T[CT][GA]AG
763 CTCGAG
TfiI 194 GAATC G^A[AT]TC
314 GAATC
XhoII 104 GGATCC [GA]^GATC[CT]
164 AGATCT
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BanII BbvI BmyI BsaJI BsgI
BsmFI BsoBI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI MboI
MboII MnlI MspA1I MvaI MvnI
MwoI NdeII NlaIV Sau3AI Sau96I
ScrFI SfaNI TaqI TseI TthHB8I