Restriction map for
pFN21AE4307
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI EarI Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AhdI 145 GACCGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 744 GATCC GGATC(4/5)
AspEI 145 GACCGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 802 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 858 CCCGGG C^[CT]CG[GA]G
BanI 111 GGTGCC G^G[CT][GA]CC
BbsI 478 GAAGAC GAAGAC(2/6)
BpuAI 478 GAAGAC GAAGAC(2/6)
BsiEI 865 CGGCCG CG[GA][CT]^CG
BsmI 751 GCATTC GAATGC(1/-1)
BsoBI 858 CCCGGG C^[CT]CG[GA]G
BsrFI 922 GCCGGC [GA]^CCGG[CT]
BstF5I 5 GGATG GGATG(2/0)
BstXI 115 CCAGTCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 743 AGATCC [GA]^GATC[CT]
Cfr10I 922 GCCGGC [GA]^CCGG[CT]
DpnII 744 GATC ^GATC
DraIII 262 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 865 CGGCCG C^GGCCG
Eam1105I 145 GACCGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 865 CGGCCG C^GGCCG
Eco52I 865 CGGCCG C^GGCCG
FokI 5 GGATG GGATG(9/13)
HaeII 15 GGCGCT [GA]GCGC^[CT]
HgaI 203 GACGC GACGC(5/10)
KspI 32 CCGCGG CCGC^GG
MaeII 238 ACGT A^CGT
MboI 744 GATC ^GATC
MflI 743 AGATCC [GA]^GATC[CT]
NaeI 922 GCCGGC GCC^GGC
NcoI 407 CCATGG C^CATGG
NdeII 744 GATC ^GATC
NgoMIV 922 GCCGGC G^CCGGC
PflFI 802 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 540 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 470 AGGACCC [GA]G^G[AT]CC[CT]
PstI 817 CTGCAG CTGCA^G
PvuII 823 CAGCTG CAG^CTG
SacII 32 CCGCGG CCGC^GG
Sau3AI 744 GATC ^GATC
SfcI 817 CTGCAG C^T[GA][CT]AG
SmaI 858 CCCGGG CCC^GGG
Tsp509I 486 AATT ^AATT
Tth111I 802 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 540 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 176 CCAAGGCAAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 743 AGATCC [GA]^GATC[CT]
XmaCI 858 CCCGGG C^CCGGG
XmaI 858 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AccII 33 CGCG CG^CG
38 CGCG
AfaI 424 GTAC GT^AC
768 GTAC
BpmI 280 CTGGAG CTGGAG(16/14)
302 CTGGAG
BseRI 305 GAGGAG GAGGAG(10/8)
631 GAGGAG
BsgI 346 CTGCAC GTGCAG(16/14)
53 GTGCAG
BstUI 33 CGCG CG^CG
38 CGCG
DsaI 32 CCGCGG C^C[GA][CT]GG
407 CCATGG
HinfI 58 GAGTC G^A[ACGT]TC
88 GAGTC
MaeIII 60 GTCAC ^GT[ACGT]AC
169 GTCAC
MvnI 33 CGCG CG^CG
38 CGCG
PleI 58 GAGTC GAGTC(4/5)
88 GAGTC
RsaI 424 GTAC GT^AC
768 GTAC
SfaNI 6 GATGC GCATC(5/9)
414 GATGC
TaqI 389 TCGA T^CGA
448 TCGA
Tsp45I 60 GTCAC ^GT[GC]AC
169 GTCAC
TthHB8I 389 TCGA T^CGA
448 TCGA
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AlwNI ApaI AspHI
AvaII BanII BbvI BcnI BmyI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BspMI BsrI BssKI BstNI
Cac8I CfoI Cfr13I DdeI DraII
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MboII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV Sau96I ScrFI StyI
TseI TspRI