Restriction map for
pFN21AE1619
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# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BbeI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BpmI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstXI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII HphI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MspI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
TaqI Tru9I Tsp509I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AlwI 183 GGATC GGATC(4/5)
AlwNI 106 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 143 GGGCCC GGGCC^C
AspHI 121 GAGCTC G[AT]GC[AT]^C
AvaII 231 GGACC G^G[AT]CC
BanI 294 GGTGCC G^G[CT][GA]CC
BbrPI 318 CACGTG CAC^GTG
BbsI 156 GAAGAC GAAGAC(2/6)
BfaI 220 CTAG C^TAG
BlpI 18 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 18 GCTGAGC GC^T[ACGT]AGC
BpuAI 156 GAAGAC GAAGAC(2/6)
BsaAI 318 CACGTG [CT]AC^GT[GA]
BsiHKAI 121 GAGCTC G[AT]GC[AT]^C
BsmFI 285 GGGAC GGGAC(10/14)
BsrBI 243 CCGCTC CCGCTC(-3/-3)
BstAPI 40 GCACTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 183 GGATCT [GA]^GATC[CT]
CelII 18 GCTGAGC GC^T[ACGT]AGC
DpnII 184 GATC ^GATC
DsaI 80 CCATGG C^C[GA][CT]GG
EarI 178 GAAGAG CTCTTC(1/4)
Ksp632I 178 GAAGAG CTCTTC(1/4)
MaeI 220 CTAG C^TAG
MaeIII 305 GTGAC ^GT[ACGT]AC
MboI 184 GATC ^GATC
MflI 183 GGATCT [GA]^GATC[CT]
MspA1I 11 CAGCGG C[AC]G^C[GT]G
NcoI 80 CCATGG C^CATGG
NdeII 184 GATC ^GATC
PleI 274 GAGTC GAGTC(4/5)
PmaCI 318 CACGTG CAC^GTG
PmlI 318 CACGTG CAC^GTG
SacI 121 GAGCTC GAGCT^C
Sau3AI 184 GATC ^GATC
SbfI 225 CCTGCAGG CCTGCA^GG
SfaNI 89 GCATC GCATC(5/9)
Sse8387I 225 CCTGCAGG CCTGCA^GG
TfiI 378 GAATC G^A[AT]TC
Tsp45I 305 GTGAC ^GT[GC]AC
XhoII 183 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccII 53 CGCG CG^CG
235 CGCG
AfaI 217 GTAC GT^AC
368 GTAC
BseRI 246 CTCCTC GAGGAG(10/8)
56 GAGGAG
BspMI 224 ACCTGC ACCTGC(4/8)
291 GCAGGT
BstUI 53 CGCG CG^CG
235 CGCG
DdeI 19 CTGAG C^T[ACGT]AG
200 CTCAG
DraII 107 AGGCCCT [GA]G^G[ACGT]CC[CT]
143 GGGCCCC
EcoO109I 107 AGGCCCT [GA]G^G[ACGT]CC[CT]
143 GGGCCCC
EcoT14I 80 CCATGG C^C[AT][AT]GG
206 CCTTGG
FauI 242 CCCGC CCCGC(4/6)
141 GCGGG
HinfI 274 GAGTC G^A[ACGT]TC
378 GAATC
MaeII 252 ACGT A^CGT
319 ACGT
MvnI 53 CGCG CG^CG
235 CGCG
RsaI 217 GTAC GT^AC
368 GTAC
StyI 80 CCATGG C^C[AT][AT]GG
206 CCTTGG
TspRI 41 CACTG CA[GC]TG[ACGT][ACGT]^
149 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstNI Cac8I Cfr13I EcoRII Fnu4HI
HaeIII ItaI MboII MnlI MvaI
MwoI NlaIII NlaIV PstI Sau96I
ScrFI SfcI StuI TseI