Restriction map for
pFN21AB8502
##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AviII BamHI BbrPI BbvCI
BcgI BclI BfrI BsaAI BsaBI
BsaWI BseAI BspDI BspEI BspLU11I
BsrDI Bst1107I BstZ17I ClaI CpoI
DraI Eam1105I Eco47III EcoT22I FbaI
FspI HincII HindII HpaI KpnI
MamI MfeI MluI MroI MseI
MunI NdeI NotI NruI NsiI
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SalI
SfiI SgrAI SnaBI SpeI SphI
SspI SwaI Tru9I XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 831 GACGTC GACGT^C
AlwI 1122 GATCC GGATC(4/5)
Asp700I 1794 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2200 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2233 CCTAGG C^CTAGG
BbsI 424 GTCTTC GAAGAC(2/6)
BciVI 1048 GTATCC GTATCC(6/5)
BlnI 2233 CCTAGG C^CTAGG
BpuAI 424 GTCTTC GAAGAC(2/6)
BsaI 451 GGTCTC GGTCTC(1/5)
BsiWI 266 CGTACG C^GTACG
BsmBI 429 CGTCTC CGTCTC(1/5)
BsmI 1195 GAATGC GAATGC(1/-1)
Bsp1407I 467 TGTACA T^GTACA
BspHI 2264 TCATGA T^CATGA
BsrGI 467 TGTACA T^GTACA
BssHII 660 GCGCGC G^CGCGC
BstBI 1415 TTCGAA TT^CGAA
BstEII 288 GGTGACC G^GT[ACGT]ACC
BstPI 288 GGTGACC G^GT[ACGT]ACC
DrdI 127 GACCCGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 288 GGTGACC G^GT[ACGT]ACC
EcoRV 2008 GATATC GAT^ATC
FseI 855 GGCCGGCC GGCCGG^CC
HindIII 1411 AAGCTT A^AGCTT
NcoI 1551 CCATGG C^CATGG
NheI 1221 GCTAGC G^CTAGC
NspI 2162 ACATGC [GA]CATG^[CT]
NspV 1415 TTCGAA TT^CGAA
PaeR7I 548 CTCGAG C^TCGAG
PflFI 2200 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1893 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 2264 TCATGA T^CATGA
ScaI 2333 AGTACT AGT^ACT
SexAI 1497 ACCAGGT A^CC[AT]GGT
SfuI 1415 TTCGAA TT^CGAA
SspBI 467 TGTACA T^GTACA
Tth111I 2200 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1893 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 548 CTCGAG C^TCGAG
XmnI 1794 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 177 GAATTC [GA]^AATT[CT]
2361 GAATTC
ApoI 177 GAATTC [GA]^AATT[CT]
2361 GAATTC
BalI 348 TGGCCA TGG^CCA
1976 TGGCCA
BglI 1205 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2501 GCCCTCTTGGC
BglII 2144 AGATCT A^GATCT
2428 AGATCT
BseRI 2213 GAGGAG GAGGAG(10/8)
2398 GAGGAG
BssSI 2003 CACGAG CACGAG(-5/-1)
2284 CACGAG
BstAPI 1142 GCAGTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1362 GCAGGGCCTGC
DraIII 251 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
1496 CACCAGGTG
EcoNI 713 CCTACCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1674 CCTCATGGAGG
EcoRI 177 GAATTC G^AATTC
2361 GAATTC
MluNI 348 TGGCCA TGG^CCA
1976 TGGCCA
MscI 348 TGGCCA TGG^CCA
1976 TGGCCA
MslI 471 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1239 CACCTGCATG
PshAI 496 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1603 GACTCCAGTC
SapI 559 GAAGAGC GCTCTTC(1/4)
1074 GCTCTTC
SbfI 1368 CCTGCAGG CCTGCA^GG
1614 CCTGCAGG
Sse8387I 1368 CCTGCAGG CCTGCA^GG
1614 CCTGCAGG
StuI 746 AGGCCT AGG^CCT
2078 AGGCCT
TaqI 549 TCGA T^CGA
1416 TCGA
TthHB8I 549 TCGA T^CGA
1416 TCGA
XcmI 1458 CCAGGTGGGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1551 CCATGGTGCCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AocI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaHI BsaJI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StyI TfiI TseI
Tsp45I Tsp509I TspRI XhoII XmaCI
XmaI